While working on CPJUMP-Stain2, @shntnu and I observed that the proportion of compounds with a strong signal (percent strong metric) was similar if the analysis was performed with individual channels or across all channels. We wanted to find out if this behavior was seen in other datasets as well.
I chose one of the platemaps (H-BIOA-002-1) from BBBC022 and computed the channel-wise correlation values. Based on the results below, it looks like BBBC022 also behaves similarly.
BBBC022 channel-wise percent strong comparison
Click to expand!
BBBBC022 all channels percent strong
Click to expand!
Performing this experiment with a larger dataset, such as LINCS, may help answer whether the above plots are technical artifacts or if this behavior is consistent across datasets.
While working on CPJUMP-Stain2, @shntnu and I observed that the proportion of compounds with a strong signal (percent strong metric) was similar if the analysis was performed with individual channels or across all channels. We wanted to find out if this behavior was seen in other datasets as well.
I chose one of the platemaps (H-BIOA-002-1) from BBBC022 and computed the channel-wise correlation values. Based on the results below, it looks like BBBC022 also behaves similarly.
BBBC022 channel-wise percent strong comparison
Click to expand!
BBBBC022 all channels percent strong
Click to expand!
Performing this experiment with a larger dataset, such as LINCS, may help answer whether the above plots are technical artifacts or if this behavior is consistent across datasets.