Figure 4. Cell Painting morphology features are more redundant than L1000 gene expression features. (a) Feature heatmaps for 480 Cell Painting features and 974 L1000 features derived from feature selected consensus signatures. (b) Transcriptional and morphological activity scores across dose for all common compounds indicates how frequently and consistently perturbations disrupted either L1000 or Cell Painting features. (c) The percentage of variance explained for the top 30 principal components derived from a Principal Component Analysis (PCA) in Cell Painting or L1000 readouts. (d) Comparing activity scores for highly reproducible compound perturbations reveals that most compounds induce morphological changes to a higher degree than gene expression changes. (e) Overrepresentation analysis (ORA) of the genes associated with each compound independently reveals certain gene ontology (GO) gene sets associated with compounds that induce high morphological changes.
Associated with #25