broadinstitute / lincs-profiling-complementarity

Analyzing and comparing signal found in different profiling technologies
BSD 3-Clause "New" or "Revised" License
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[Response to Review] Edge well filter QC percent strong #65

Closed gwaybio closed 2 years ago

gwaybio commented 2 years ago

A reviewer asked us to perform some form of quality control to filter profiles prior to analysis.

This is not a typical step in the image-based profiling pipeline, but it is important to compare metrics if we perform some QC. It does not make sense to filter profiles based on cell count (see #63), but it does make sense to filter profiles in edge wells given our well position effect observations.

In this PR, I calculate percent strong after filtering edge wells in both assays. I add two rows and two bars to the following supplementary figure:

supfigure4