broadinstitute / pooled-cell-painting-profiling-recipe

:woman_cook: Recipe repository for image-based profiling of Pooled Cell Painting experiments
BSD 3-Clause "New" or "Revised" License
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Add optional extra barcode preprocessing #93

Open ErinWeisbart opened 9 months ago

ErinWeisbart commented 9 months ago

Currently, the barcodes called, quality of barcodes called, and sgRNA assigned to barcode are all output by CellProfiler with no option to modify in-recipe. It would be helpful to be able to perform troubleshooting to optimize barcode calling in recipe by enabling and additional barcode processing step that reads barcodes called but overwrites quality assignment and sgRNA assignment to allow for:

ErinWeisbart commented 9 months ago

This is complemented by option to drop individual barcodes https://github.com/broadinstitute/pooled-cell-painting-profiling-template/commit/ba4e4ed582c2934de221f520ab282c9555b9722c