Currently, the barcodes called, quality of barcodes called, and sgRNA assigned to barcode are all output by CellProfiler with no option to modify in-recipe.
It would be helpful to be able to perform troubleshooting to optimize barcode calling in recipe by enabling and additional barcode processing step that reads barcodes called but overwrites quality assignment and sgRNA assignment to allow for:
ignoring cycle n from all reads (e.g. we know Cycle 4 had a reagent mistake. Don't use Cycle 4 when determining call quality)
use N cycles (e.g. we gathered and read all 12 cycles but we know the last two are the worst performing and want to consider call quality only from cycle 1-10)
Currently, the barcodes called, quality of barcodes called, and sgRNA assigned to barcode are all output by CellProfiler with no option to modify in-recipe. It would be helpful to be able to perform troubleshooting to optimize barcode calling in recipe by enabling and additional barcode processing step that reads barcodes called but overwrites quality assignment and sgRNA assignment to allow for: