gwaybio
commented
4 years ago - Data from merged batches 5, 6, and 7 looking good so far
- Batch correction not necessary
- There might be some minor well effects
- We discussed randomizing platemaps to the extent possible
- Morphology signature is interesting, and worth having a biologist dig a bit deeper into
- Still some concern over confluence issues
- While it doesn't seem to impact profiles, it is impacting explanatory morphology features that are typically only important in high confluence settings
- There might be something additionally interesting about the way the clones were selected
- We talked about how they were all selected from the same cluster of resistant clones
- Can you check me on this @mekelley? It is likely I didn't truly appreciate this point and I think it is important. (if possible, please add this to a new issue similar to #45 )
- We know which cluster each sample belongs to which will help with:
- Sequence specific clusters to determine molecular resistance mechanism
- Use cluster info in future covariate models to observe which features are important for cluster of origin
- The Rockefeller team is actively onboarding new clones and a new proteasome inhibitor
- They will present at next check-in
- We also briefly discussed presentations at next CSHL
- It will be analysis heavy and @AnneCarpenter will represent (I don't remember if we've discussed this elsewhere before!)