Closed tomkinsc closed 5 years ago
In WDL:
minimum_base_quality
25
max_reads_in_ram_per_tile
max_records_in_ram
In py tool defaults for picard:
In WDL:
minimum_base_quality
for barcode matching to25
except for NovaSeqmax_reads_in_ram_per_tile
andmax_records_in_ram
for NextSeq to account for larger tile size (avoid running out of open file handles)In py tool defaults for picard:
minimum_base_quality
from 10 to 20 for more stringent barcode Q score threshold (while still allowing two levels of NovaSeq quality)