Hi Michael,
Is there a cutoff when deciding a sample has a good quality score. Usually what would be the approximate quality score (range) we should get for a high quality sample. And when use multiple resolution values (-r) when running the tool, with fixed size bins in interaction matrices (i.e 50kb) how does it effect the quality score. This is what the documentations says,
"A simple quality or replicate score can be determined with a single resolution and coverage value, although testing across multiple resolutions and be used to determine sample resolution limits while multiple coverages can be used to model quality as a function of sequencing depth and determine the maximum sample quality score". Does it mean, we can use this quality score to determine whether we can go for a lower resolution (bin size) when creating the interaction matrices? This is what I understood from the paper. (Maximum usable resolution)
Thank You
Hi Michael, Is there a cutoff when deciding a sample has a good quality score. Usually what would be the approximate quality score (range) we should get for a high quality sample. And when use multiple resolution values (-r) when running the tool, with fixed size bins in interaction matrices (i.e 50kb) how does it effect the quality score. This is what the documentations says, "A simple quality or replicate score can be determined with a single resolution and coverage value, although testing across multiple resolutions and be used to determine sample resolution limits while multiple coverages can be used to model quality as a function of sequencing depth and determine the maximum sample quality score". Does it mean, we can use this quality score to determine whether we can go for a lower resolution (bin size) when creating the interaction matrices? This is what I understood from the paper. (Maximum usable resolution) Thank You