Something went wrong with running MaxBin2

[zl945]

input: metawrap binning -o INITIAL_BINNING4 -t 8 -m 200 -a ASSEMBLY/final_assembly.fasta --maxbin2 cleandata/Sz_1.fastq cleandata/Sz_2.fastq

metaWRAP v=1.0.2 installed by conda

error：

metawrap binning -o INITIAL_BINNING4 -t 8 -m 200 -a ASSEMBLY/final_assembly.fasta --maxbin2 cleandata/Sz_1.fastq cleandata/Sz_2.fastq

------------------------------------------------------------------------------------------------------------------------
-----                                  1 forward and 1 reverse read files detected                                 -----
------------------------------------------------------------------------------------------------------------------------

########################################################################################################################
#####                                     ALIGNING READS TO MAKE COVERAGE FILES                                    #####
########################################################################################################################

------------------------------------------------------------------------------------------------------------------------
-----                          making copy of assembly file ASSEMBLY/final_assembly.fasta                          -----
------------------------------------------------------------------------------------------------------------------------

------------------------------------------------------------------------------------------------------------------------
-----                                            Indexing assembly file                                            -----
------------------------------------------------------------------------------------------------------------------------

[bwa_index] Pack FASTA... 1.92 sec
[bwa_index] Construct BWT for the packed sequence...
[BWTIncCreate] textLength=419247880, availableWord=41499380
[BWTIncConstructFromPacked] 10 iterations done. 68401368 characters processed.
[BWTIncConstructFromPacked] 20 iterations done. 126419640 characters processed.
[BWTIncConstructFromPacked] 30 iterations done. 177982392 characters processed.
[BWTIncConstructFromPacked] 40 iterations done. 223807512 characters processed.
[BWTIncConstructFromPacked] 50 iterations done. 264532952 characters processed.
[BWTIncConstructFromPacked] 60 iterations done. 300725816 characters processed.
[BWTIncConstructFromPacked] 70 iterations done. 332890072 characters processed.
[BWTIncConstructFromPacked] 80 iterations done. 361473736 characters processed.
[BWTIncConstructFromPacked] 90 iterations done. 386874952 characters processed.
[BWTIncConstructFromPacked] 100 iterations done. 409447576 characters processed.
[bwa_index] 137.69 seconds elapse.
[bwa_index] Update BWT... 1.39 sec
[bwa_index] Pack forward-only FASTA... 1.07 sec
[bwa_index] Construct SA from BWT and Occ... 61.40 sec
[bwt_gen] Finished constructing BWT in 105 iterations.
[main] Version: 0.7.15-r1140
[main] CMD: bwa index INITIAL_BINNING4/work_files/assembly.fa
[main] Real time: 206.294 sec; CPU: 203.485 sec

------------------------------------------------------------------------------------------------------------------------
-----             Aligning cleandata/Sz_1.fastq and cleandata/Sz_2.fastq back to assembly, sorting the             -----
-----                            alignment, and gathering statistics on insert lengths                             -----
------------------------------------------------------------------------------------------------------------------------

[M::bwa_idx_load_from_disk] read 0 ALT contigs
[M::process] read 530908 sequences (80000272 bp)...
[M::process] read 530862 sequences (80000245 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 162584, 45, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.52, 90.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (70, 316, 2476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7288)
[M::mem_pestat] mean and std.dev: (1310.74, 2110.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9754)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530908 reads in 92.564 CPU sec, 12.244 real sec
[M::process] read 530910 sequences (80000232 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161153, 50, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 666)
[M::mem_pestat] mean and std.dev: (367.46, 90.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 470, 2143)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6105)
[M::mem_pestat] mean and std.dev: (986.76, 1297.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8086)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530862 reads in 96.214 CPU sec, 11.905 real sec
[M::process] read 530846 sequences (80000227 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161812, 45, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 666)
[M::mem_pestat] mean and std.dev: (367.76, 90.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 422, 1338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800)
[M::mem_pestat] mean and std.dev: (430.39, 483.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5031)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530910 reads in 95.915 CPU sec, 12.133 real sec
[M::process] read 530896 sequences (80000155 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161774, 47, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.30, 90.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 450, 1182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166)
[M::mem_pestat] mean and std.dev: (615.07, 698.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4158)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530846 reads in 98.658 CPU sec, 12.047 real sec
[M::process] read 530910 sequences (80000094 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161327, 53, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.45, 90.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 394, 1718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4704)
[M::mem_pestat] mean and std.dev: (528.47, 634.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6197)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530896 reads in 95.201 CPU sec, 11.759 real sec
[M::process] read 530894 sequences (80000051 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161684, 33, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 663)
[M::mem_pestat] mean and std.dev: (367.28, 90.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 1078, 5409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15645)
[M::mem_pestat] mean and std.dev: (2372.70, 2572.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20763)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530910 reads in 97.327 CPU sec, 12.023 real sec
[M::process] read 530960 sequences (80000130 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161966, 49, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (367.51, 90.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 554, 2087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5933)
[M::mem_pestat] mean and std.dev: (1004.15, 1284.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7856)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530894 reads in 94.405 CPU sec, 11.598 real sec
[M::process] read 530832 sequences (80000297 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161969, 43, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (367.49, 90.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 563, 3219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9117)
[M::mem_pestat] mean and std.dev: (1964.40, 2330.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12066)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530960 reads in 94.986 CPU sec, 11.751 real sec
[M::process] read 530798 sequences (80000125 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161556, 58, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (367.97, 90.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 571, 1980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5460)
[M::mem_pestat] mean and std.dev: (816.49, 1109.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7200)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530832 reads in 94.598 CPU sec, 11.699 real sec
[M::process] read 530892 sequences (80000108 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161590, 48, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.52, 90.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 538, 1642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4596)
[M::mem_pestat] mean and std.dev: (941.36, 1146.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6073)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530798 reads in 94.374 CPU sec, 11.642 real sec
[M::process] read 530846 sequences (80000222 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161078, 54, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.35, 90.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 309, 1014)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714)
[M::mem_pestat] mean and std.dev: (593.40, 739.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3564)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530892 reads in 94.710 CPU sec, 11.720 real sec
[M::process] read 530876 sequences (80000301 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161575, 49, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.55, 90.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 419, 1635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455)
[M::mem_pestat] mean and std.dev: (691.59, 873.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5865)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530846 reads in 94.750 CPU sec, 11.714 real sec
[M::process] read 530882 sequences (80000205 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 161923, 60, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 666)
[M::mem_pestat] mean and std.dev: (367.88, 90.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 451, 2120)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5964)
[M::mem_pestat] mean and std.dev: (1118.50, 1487.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7886)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530876 reads in 94.468 CPU sec, 11.652 real sec
[M::process] read 530870 sequences (80000005 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 161794, 67, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 663)
[M::mem_pestat] mean and std.dev: (367.27, 90.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 363, 1854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5190)
[M::mem_pestat] mean and std.dev: (862.27, 1182.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6858)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530882 reads in 95.665 CPU sec, 11.789 real sec
[M::process] read 530930 sequences (80000168 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161619, 33, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 363, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664)
[M::mem_pestat] mean and std.dev: (368.21, 90.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 1118, 3647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10553)
[M::mem_pestat] mean and std.dev: (1979.39, 2214.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14006)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530870 reads in 95.009 CPU sec, 11.732 real sec
[M::process] read 530760 sequences (80000278 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 162248, 43, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.18, 90.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (394, 548, 1743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4441)
[M::mem_pestat] mean and std.dev: (598.91, 665.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5790)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (106, 173, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (226.00, 132.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 530930 reads in 94.800 CPU sec, 11.673 real sec
[M::process] read 530820 sequences (80000137 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 161494, 46, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.30, 90.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 691, 1487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4005)
[M::mem_pestat] mean and std.dev: (775.40, 760.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530760 reads in 93.131 CPU sec, 11.532 real sec
[M::process] read 530868 sequences (80000171 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161689, 36, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.40, 90.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 570, 834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244)
[M::mem_pestat] mean and std.dev: (424.69, 394.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2949)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530820 reads in 93.632 CPU sec, 11.783 real sec
[M::process] read 530852 sequences (80000020 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161813, 55, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.30, 90.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 501, 1341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3627)
[M::mem_pestat] mean and std.dev: (680.90, 755.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4770)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530868 reads in 94.042 CPU sec, 11.649 real sec
[M::process] read 530866 sequences (80000177 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161568, 53, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.93, 90.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (450, 790, 2558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6774)
[M::mem_pestat] mean and std.dev: (1482.31, 1722.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8882)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530852 reads in 96.682 CPU sec, 11.963 real sec
[M::process] read 530850 sequences (80000200 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162007, 50, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.09, 90.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 559, 2145)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6127)
[M::mem_pestat] mean and std.dev: (1016.83, 1295.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8118)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530866 reads in 95.196 CPU sec, 11.780 real sec
[M::process] read 530864 sequences (80000218 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161949, 50, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.67, 90.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 519, 1803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4955)
[M::mem_pestat] mean and std.dev: (680.33, 754.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6531)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530850 reads in 96.729 CPU sec, 11.927 real sec
[M::process] read 531090 sequences (80000098 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 161964, 45, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.63, 91.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 593, 2316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6770)
[M::mem_pestat] mean and std.dev: (1243.25, 1590.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8997)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530864 reads in 94.548 CPU sec, 11.702 real sec
[M::process] read 530796 sequences (80000024 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161294, 44, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 666)
[M::mem_pestat] mean and std.dev: (367.64, 90.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 542, 2117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5989)
[M::mem_pestat] mean and std.dev: (1102.17, 1515.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7925)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531090 reads in 95.284 CPU sec, 11.796 real sec
[M::process] read 530806 sequences (80000283 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162276, 54, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670)
[M::mem_pestat] mean and std.dev: (369.19, 91.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 567, 2134)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6134)
[M::mem_pestat] mean and std.dev: (973.23, 1296.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8134)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530796 reads in 94.516 CPU sec, 11.846 real sec
[M::process] read 530856 sequences (80000235 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 162264, 47, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.99, 90.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 495, 1976)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5532)
[M::mem_pestat] mean and std.dev: (969.95, 1124.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7310)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530806 reads in 95.461 CPU sec, 11.775 real sec
[M::process] read 530822 sequences (80000080 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161852, 49, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.87, 90.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 547, 2408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6828)
[M::mem_pestat] mean and std.dev: (1221.64, 1569.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9038)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530856 reads in 94.227 CPU sec, 11.668 real sec
[M::process] read 530810 sequences (80000119 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161523, 63, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670)
[M::mem_pestat] mean and std.dev: (369.53, 90.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 473, 2171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6243)
[M::mem_pestat] mean and std.dev: (1061.34, 1403.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8279)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530822 reads in 94.437 CPU sec, 11.676 real sec
[M::process] read 530840 sequences (80000123 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 161448, 40, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.40, 90.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 593, 1784)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4910)
[M::mem_pestat] mean and std.dev: (790.69, 914.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6473)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530810 reads in 96.237 CPU sec, 11.866 real sec
[M::process] read 530910 sequences (80000232 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 162063, 55, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.71, 90.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 494, 2400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6870)
[M::mem_pestat] mean and std.dev: (1074.90, 1472.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9105)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530840 reads in 94.446 CPU sec, 11.694 real sec
[M::process] read 530896 sequences (80000184 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161880, 60, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.51, 90.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 471, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670)
[M::mem_pestat] mean and std.dev: (373.28, 274.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2171)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530910 reads in 94.949 CPU sec, 11.694 real sec
[M::process] read 530824 sequences (80000102 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161599, 49, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 666)
[M::mem_pestat] mean and std.dev: (367.76, 90.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 485, 1398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3806)
[M::mem_pestat] mean and std.dev: (733.44, 772.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5010)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530896 reads in 94.000 CPU sec, 11.644 real sec
[M::process] read 530882 sequences (80000124 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161639, 58, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670)
[M::mem_pestat] mean and std.dev: (369.03, 90.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 650, 2165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6021)
[M::mem_pestat] mean and std.dev: (942.55, 1049.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7949)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530824 reads in 94.441 CPU sec, 11.655 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 161381, 64, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.87, 90.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 309, 1214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3426)
[M::mem_pestat] mean and std.dev: (580.46, 696.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4532)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530882 reads in 105.997 CPU sec, 13.182 real sec
[M::process] read 530830 sequences (80000037 bp)...
[M::process] read 530832 sequences (80000202 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161543, 53, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670)
[M::mem_pestat] mean and std.dev: (369.22, 90.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 517, 2595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7411)
[M::mem_pestat] mean and std.dev: (1142.23, 1372.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9819)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530830 reads in 93.196 CPU sec, 11.616 real sec
[M::process] read 530906 sequences (80000101 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161516, 55, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (308, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668)
[M::mem_pestat] mean and std.dev: (369.25, 90.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 402, 984)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434)
[M::mem_pestat] mean and std.dev: (488.63, 438.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3159)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530832 reads in 96.008 CPU sec, 11.857 real sec
[M::process] read 530854 sequences (80000169 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161126, 48, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.77, 90.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 319, 717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907)
[M::mem_pestat] mean and std.dev: (370.26, 374.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2502)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530906 reads in 96.453 CPU sec, 11.925 real sec
[M::process] read 530920 sequences (80000109 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 162137, 43, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.45, 90.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 689, 1816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5108)
[M::mem_pestat] mean and std.dev: (909.76, 1186.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6754)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530854 reads in 95.303 CPU sec, 11.794 real sec
[M::process] read 530968 sequences (80000143 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161444, 57, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.26, 90.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 469, 1618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4498)
[M::mem_pestat] mean and std.dev: (640.65, 806.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5938)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530920 reads in 94.605 CPU sec, 11.699 real sec
[M::process] read 530862 sequences (80000186 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161473, 57, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.84, 90.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 489, 2237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6423)
[M::mem_pestat] mean and std.dev: (593.62, 760.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8516)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530968 reads in 95.205 CPU sec, 11.777 real sec
[M::process] read 530996 sequences (80000245 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 162366, 46, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667)
[M::mem_pestat] mean and std.dev: (368.46, 90.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 423, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1374)
[M::mem_pestat] mean and std.dev: (328.48, 227.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1779)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530862 reads in 94.503 CPU sec, 11.702 real sec
[M::process] read 530962 sequences (80000294 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162325, 44, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670)
[M::mem_pestat] mean and std.dev: (369.49, 90.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 549, 960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498)
[M::mem_pestat] mean and std.dev: (393.80, 296.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3267)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530996 reads in 94.152 CPU sec, 11.649 real sec
[M::process] read 530914 sequences (80000216 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161567, 53, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 669)
[M::mem_pestat] mean and std.dev: (368.41, 90.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 425, 2032)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5842)
[M::mem_pestat] mean and std.dev: (950.00, 1163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7747)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530962 reads in 94.202 CPU sec, 11.619 real sec
[M::process] read 530916 sequences (80000166 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161815, 60, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (308, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668)
[M::mem_pestat] mean and std.dev: (369.50, 90.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (284, 618, 2029)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5519)
[M::mem_pestat] mean and std.dev: (1057.91, 1138.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7264)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530914 reads in 94.689 CPU sec, 11.719 real sec
[M::process] read 530948 sequences (80000020 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161743, 54, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 364, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670)
[M::mem_pestat] mean and std.dev: (369.18, 90.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 600, 1921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5509)
[M::mem_pestat] mean and std.dev: (1105.37, 1295.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7303)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530916 reads in 94.053 CPU sec, 11.624 real sec
[M::process] read 531118 sequences (80000166 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 161801, 55, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (306, 363, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 666)
[M::mem_pestat] mean and std.dev: (368.14, 90.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 409, 2362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6878)
[M::mem_pestat] mean and std.dev: (1231.60, 1519.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9136)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 530948 reads in 93.918 CPU sec, 11.606 real sec
[M::process] read 531376 sequences (80000093 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 161334, 46, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.99, 90.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 888, 2963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8203)
[M::mem_pestat] mean and std.dev: (1843.28, 2162.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10823)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531118 reads in 94.666 CPU sec, 11.701 real sec
[M::process] read 531308 sequences (80000099 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162743, 50, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 662)
[M::mem_pestat] mean and std.dev: (366.20, 90.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 460, 1946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5614)
[M::mem_pestat] mean and std.dev: (832.89, 1054.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7448)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531376 reads in 94.907 CPU sec, 11.737 real sec
[M::process] read 531196 sequences (80000294 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162515, 51, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 360, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 661)
[M::mem_pestat] mean and std.dev: (365.53, 90.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 505, 1410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3852)
[M::mem_pestat] mean and std.dev: (630.48, 870.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5073)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531308 reads in 92.747 CPU sec, 11.470 real sec
[M::process] read 531196 sequences (80000118 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 162039, 60, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.88, 90.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 510, 1645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4539)
[M::mem_pestat] mean and std.dev: (781.79, 969.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5986)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531196 reads in 92.884 CPU sec, 11.507 real sec
[M::process] read 531130 sequences (80000070 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162168, 46, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.83, 90.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 580, 2029)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5715)
[M::mem_pestat] mean and std.dev: (999.05, 1393.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7558)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531196 reads in 93.578 CPU sec, 11.576 real sec
[M::process] read 531152 sequences (80000083 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161852, 48, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 360, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 661)
[M::mem_pestat] mean and std.dev: (365.55, 89.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 418, 2480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7142)
[M::mem_pestat] mean and std.dev: (1187.50, 1493.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9473)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531130 reads in 95.564 CPU sec, 11.826 real sec
[M::process] read 531136 sequences (80000281 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162674, 52, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (366.05, 90.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 411, 1672)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4542)
[M::mem_pestat] mean and std.dev: (714.33, 762.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5977)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531152 reads in 93.811 CPU sec, 11.615 real sec
[M::process] read 531342 sequences (80000230 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 162176, 50, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.93, 90.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 421, 1162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3090)
[M::mem_pestat] mean and std.dev: (493.80, 565.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4054)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531136 reads in 94.720 CPU sec, 11.729 real sec
[M::process] read 531370 sequences (80000205 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162096, 40, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 360, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 661)
[M::mem_pestat] mean and std.dev: (365.41, 90.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (81, 428, 1621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4701)
[M::mem_pestat] mean and std.dev: (803.84, 1190.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6241)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531342 reads in 94.864 CPU sec, 11.757 real sec
[M::process] read 531292 sequences (80000054 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162886, 58, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.72, 90.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 532, 2413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6841)
[M::mem_pestat] mean and std.dev: (1274.95, 1590.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9055)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531370 reads in 93.711 CPU sec, 11.583 real sec
[M::process] read 531198 sequences (80000061 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 162424, 50, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.44, 90.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 485, 754)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (425.05, 338.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2401)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531292 reads in 95.464 CPU sec, 11.763 real sec
[M::process] read 531238 sequences (80000181 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 162156, 62, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667)
[M::mem_pestat] mean and std.dev: (366.19, 90.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 685, 1398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3660)
[M::mem_pestat] mean and std.dev: (808.24, 836.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4791)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531198 reads in 96.190 CPU sec, 11.902 real sec
[M::process] read 531244 sequences (80000254 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 162141, 66, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667)
[M::mem_pestat] mean and std.dev: (366.33, 90.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (74, 297, 793)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2231)
[M::mem_pestat] mean and std.dev: (426.98, 542.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2950)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531238 reads in 95.785 CPU sec, 11.855 real sec
[M::process] read 531158 sequences (80000268 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161184, 52, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667)
[M::mem_pestat] mean and std.dev: (366.49, 90.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 756, 2197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6235)
[M::mem_pestat] mean and std.dev: (1290.88, 1472.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8254)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531244 reads in 94.703 CPU sec, 11.718 real sec
[M::process] read 531180 sequences (80000271 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161821, 57, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667)
[M::mem_pestat] mean and std.dev: (366.30, 90.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 315, 710)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742)
[M::mem_pestat] mean and std.dev: (322.87, 249.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2258)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531158 reads in 96.769 CPU sec, 11.949 real sec
[M::process] read 531264 sequences (80000061 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161855, 57, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.54, 90.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 637, 1313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685)
[M::mem_pestat] mean and std.dev: (822.70, 948.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4871)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531180 reads in 94.501 CPU sec, 11.666 real sec
[M::process] read 531212 sequences (80000043 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 162173, 50, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.31, 90.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 419, 870)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2214)
[M::mem_pestat] mean and std.dev: (470.14, 499.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2886)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531264 reads in 94.419 CPU sec, 11.677 real sec
[M::process] read 531256 sequences (80000229 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 161857, 44, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.49, 90.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 508, 1864)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5052)
[M::mem_pestat] mean and std.dev: (827.46, 942.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6646)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531212 reads in 94.952 CPU sec, 11.740 real sec
[M::process] read 531072 sequences (80000072 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162037, 51, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.84, 90.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 415, 1854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5324)
[M::mem_pestat] mean and std.dev: (526.50, 840.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7059)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531256 reads in 95.099 CPU sec, 11.773 real sec
[M::process] read 531074 sequences (80000283 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 161711, 54, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.78, 90.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 559, 1851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5199)
[M::mem_pestat] mean and std.dev: (867.25, 994.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6873)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531072 reads in 95.064 CPU sec, 11.735 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 162664, 41, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.38, 90.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (56, 234, 762)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2174)
[M::mem_pestat] mean and std.dev: (419.55, 549.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2880)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531074 reads in 104.333 CPU sec, 13.021 real sec
[M::process] read 531124 sequences (80000079 bp)...
[M::process] read 531034 sequences (80000169 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 161858, 46, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.86, 90.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 616, 1288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3468)
[M::mem_pestat] mean and std.dev: (585.10, 558.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4558)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531124 reads in 96.273 CPU sec, 11.894 real sec
[M::process] read 531334 sequences (80000136 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 162059, 53, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667)
[M::mem_pestat] mean and std.dev: (366.28, 90.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 603, 2399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6851)
[M::mem_pestat] mean and std.dev: (1310.40, 1618.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9077)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531034 reads in 94.660 CPU sec, 11.717 real sec
[M::process] read 531220 sequences (80000223 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 161677, 53, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (366.32, 90.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 654, 1794)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4950)
[M::mem_pestat] mean and std.dev: (919.90, 1062.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6528)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531334 reads in 94.959 CPU sec, 11.769 real sec
[M::process] read 531274 sequences (80000009 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161832, 45, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.50, 90.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (303, 631, 2994)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8376)
[M::mem_pestat] mean and std.dev: (2106.93, 2837.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13455)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531220 reads in 97.092 CPU sec, 12.018 real sec
[M::process] read 531172 sequences (80000253 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 162567, 43, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.59, 90.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 485, 2450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7022)
[M::mem_pestat] mean and std.dev: (1183.00, 1481.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9308)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531274 reads in 94.043 CPU sec, 11.655 real sec
[M::process] read 531098 sequences (80000297 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161335, 48, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.66, 90.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 361, 2140)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6166)
[M::mem_pestat] mean and std.dev: (857.50, 1371.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8179)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531172 reads in 94.955 CPU sec, 11.712 real sec
[M::process] read 531090 sequences (80000004 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162003, 44, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.66, 90.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (270, 584, 4287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12321)
[M::mem_pestat] mean and std.dev: (2016.73, 2503.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16338)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531098 reads in 95.079 CPU sec, 11.770 real sec
[M::process] read 531122 sequences (80000025 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161994, 39, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (366.98, 90.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 540, 1959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5327)
[M::mem_pestat] mean and std.dev: (694.94, 746.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7011)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531090 reads in 94.814 CPU sec, 11.723 real sec
[M::process] read 531092 sequences (80000282 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161301, 47, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.65, 90.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 422, 764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1894)
[M::mem_pestat] mean and std.dev: (364.87, 284.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2459)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531122 reads in 97.471 CPU sec, 12.077 real sec
[M::process] read 531312 sequences (80000117 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 161897, 44, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.83, 90.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 615, 1907)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5291)
[M::mem_pestat] mean and std.dev: (1059.36, 1232.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6983)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531092 reads in 94.512 CPU sec, 11.679 real sec
[M::process] read 531244 sequences (80000176 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 161548, 60, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667)
[M::mem_pestat] mean and std.dev: (366.43, 90.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 340, 1160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034)
[M::mem_pestat] mean and std.dev: (518.48, 505.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3971)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531312 reads in 95.824 CPU sec, 11.855 real sec
[M::process] read 531258 sequences (80000099 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 161654, 60, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (367.47, 90.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 437, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282)
[M::mem_pestat] mean and std.dev: (382.53, 350.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3000)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531244 reads in 96.148 CPU sec, 11.883 real sec
[M::process] read 531082 sequences (80000010 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161868, 50, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (367.24, 90.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 535, 1414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734)
[M::mem_pestat] mean and std.dev: (710.72, 804.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4894)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531258 reads in 96.840 CPU sec, 12.003 real sec
[M::process] read 531106 sequences (80000136 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 161633, 53, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (367.15, 90.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 437, 1751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4845)
[M::mem_pestat] mean and std.dev: (891.92, 1145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6392)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531082 reads in 96.401 CPU sec, 11.941 real sec
[M::process] read 531096 sequences (80000183 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 162340, 57, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (367.12, 90.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 393, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660)
[M::mem_pestat] mean and std.dev: (405.57, 282.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2136)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531106 reads in 94.385 CPU sec, 11.655 real sec
[M::process] read 531544 sequences (80000277 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161569, 61, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.77, 90.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 372, 1988)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5750)
[M::mem_pestat] mean and std.dev: (546.47, 772.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7631)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531096 reads in 94.035 CPU sec, 11.636 real sec
[M::process] read 531190 sequences (80000034 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 161897, 55, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 668)
[M::mem_pestat] mean and std.dev: (367.17, 90.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 405, 1246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3456)
[M::mem_pestat] mean and std.dev: (570.51, 589.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4561)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531544 reads in 95.824 CPU sec, 11.860 real sec
[M::process] read 531306 sequences (80000140 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 162394, 56, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.98, 90.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 447, 1346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710)
[M::mem_pestat] mean and std.dev: (600.43, 740.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4892)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531190 reads in 95.827 CPU sec, 11.866 real sec
[M::process] read 531214 sequences (80000173 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 162119, 56, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.99, 90.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 422, 1458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4134)
[M::mem_pestat] mean and std.dev: (654.66, 873.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5472)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531306 reads in 98.838 CPU sec, 12.201 real sec
[M::process] read 531198 sequences (80000131 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162023, 58, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.69, 90.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 464, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886)
[M::mem_pestat] mean and std.dev: (415.44, 268.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2433)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531214 reads in 95.497 CPU sec, 11.820 real sec
[M::process] read 531010 sequences (80000089 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 162131, 46, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 662)
[M::mem_pestat] mean and std.dev: (366.06, 90.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 556, 2602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7346)
[M::mem_pestat] mean and std.dev: (1355.93, 1793.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9718)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531198 reads in 94.707 CPU sec, 11.731 real sec
[M::process] read 531030 sequences (80000056 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 162277, 35, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (367.20, 90.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 709, 2963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8025)
[M::mem_pestat] mean and std.dev: (1255.88, 1400.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10556)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531010 reads in 97.916 CPU sec, 12.097 real sec
[M::process] read 531092 sequences (80000164 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161745, 42, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.89, 90.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 517, 890)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2246)
[M::mem_pestat] mean and std.dev: (487.71, 426.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2924)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531030 reads in 95.193 CPU sec, 11.734 real sec
[M::process] read 488278 sequences (73561766 bp)...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 161842, 45, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 362, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 665)
[M::mem_pestat] mean and std.dev: (366.76, 90.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (328, 617, 2295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6229)
[M::mem_pestat] mean and std.dev: (1086.45, 1514.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8196)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 531092 reads in 97.603 CPU sec, 12.066 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 148873, 39, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (304, 361, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 664)
[M::mem_pestat] mean and std.dev: (365.95, 90.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 515, 2226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6142)
[M::mem_pestat] mean and std.dev: (1241.14, 1599.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8100)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 488278 reads in 87.904 CPU sec, 10.955 real sec
[main] Version: 0.7.15-r1140
[main] CMD: bwa mem -t 8 INITIAL_BINNING4/work_files/assembly.fa cleandata/Sz_1.fastq cleandata/Sz_2.fastq
[main] Real time: 1138.527 sec; CPU: 8768.372 sec
[bam_sort_core] merging from 16 files and 8 in-memory blocks...

########################################################################################################################
#####                                                RUNNING MAXBIN2                                               #####
########################################################################################################################

------------------------------------------------------------------------------------------------------------------------
-----                                          making contig depth file...                                         -----
------------------------------------------------------------------------------------------------------------------------

Output depth matrix to INITIAL_BINNING4/work_files/mb2_master_depth.txt
Calculating intra contig depth variance
Output matrix to INITIAL_BINNING4/work_files/mb2_master_depth.txt
Opening 1 bams
Consolidating headers
Processing bam files
Thread 0 finished: Sz.bam with 49201131 reads and 33120119 readsWellMapped
Creating depth matrix file: INITIAL_BINNING4/work_files/mb2_master_depth.txt
Closing most bam files
Closing last bam file
Finished

------------------------------------------------------------------------------------------------------------------------
-----                    split master contig depth file into individual files for maxbin2 input                    -----
------------------------------------------------------------------------------------------------------------------------

processing Sz.bam depth file...
Can't locate LWP/Simple.pm in @INC (@INC contains: /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /home/Lixiefei/miniconda2/envs/metawrap/bin/run_MaxBin.pl line 4.
BEGIN failed--compilation aborted at /home/Lixiefei/miniconda2/envs/metawrap/bin/run_MaxBin.pl line 4.

------------------------------------------------------------------------------------------------------------------------
-----             looks like our default perl libraries are not the conda ones. Manually setting perl5             -----
-----                                              library directory                                               -----
------------------------------------------------------------------------------------------------------------------------

metawrap path: /home/Lixiefei/miniconda2/envs/metawrap/bin/metawrap
Will use perl5 libraries located in /home/Lixiefei/miniconda2/envs/metawrap/lib/perl5/site_perl/5.22.0 - hopefully they are there...

------------------------------------------------------------------------------------------------------------------------
-----                                       Starting binning with MaxBin2...                                       -----
------------------------------------------------------------------------------------------------------------------------

Can't locate LWP/Simple.pm in @INC (@INC contains: /home/Lixiefei/miniconda2/envs/metawrap/lib/perl5/site_perl/5.22.0/x86_64-linux-thread-multi /home/Lixiefei/miniconda2/envs/metawrap/lib/perl5/site_perl/5.22.0 /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /home/Lixiefei/miniconda2/envs/metawrap/bin/run_MaxBin.pl line 4.
BEGIN failed--compilation aborted at /home/Lixiefei/miniconda2/envs/metawrap/bin/run_MaxBin.pl line 4.

************************************************************************************************************************
*****                              Something went wrong with running MaxBin2. Exiting.                             *****
************************************************************************************************************************

real    26m35.083s
user    212m51.633s
sys 4m36.910s

Result file:

![1551063178 1](https://user-images.githubusercontent.com/47781232/53311366-92a0ea00-38eb-11e9-8560-9dc551d7a5a1.jpg)
![1551063161 1](https://user-images.githubusercontent.com/47781232/53311370-992f6180-38eb-11e9-93ad-bfea7c7c69ca.jpg)

[ursky]

MaxBin2 requires a few Perl modules to be installed, which should be handled by conda. It looks like the Perl libraries in your system are imported by default, however. Metawrap tried to explicitly set where to looks for these libraries - /home/Lixiefei/miniconda2/envs/metawrap/lib/perl5/site_perl/5.22.0. Can you check if the conda Perl libraries are in this path or somewhere else?

[zl945]

Thanks, i changed the path of the conda Perl libraries. Now ,it works.

[ursky]

Out of curiosity, what did you change them to?

[zl945]

I am so sorry to reply to you now. Follow you suggestion, i checked the Perl modules and found the path /home/Lixiefei/miniconda2/envs/metawrap/lib/perl5/site_perl/5.22.0. Then i I linked this path to the conda manually.

[ursky]

But how did you link them?

[morgvevans]

I'm now having the same issue- but am not sure how to fix it. I found the path /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/perl5/site_perl/5.22.0 but what do i do now?? here's a copy of the error code below...

processing ALL_READS.bam depth file...
Can't locate HTTP/Status.pm in @INC (you may need to install the HTTP::Status module) (@INC contains: /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2/x86_64-linux-thread-multi /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2 /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/5.26.2/x86_64-linux-thread-multi /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/5.26.2 .) at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2/LWP/Simple.pm line 14.
BEGIN failed--compilation aborted at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2/LWP/Simple.pm line 14.
Compilation failed in require at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/bin/run_MaxBin.pl line 4.
BEGIN failed--compilation aborted at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/bin/run_MaxBin.pl line 4.

------------------------------------------------------------------------------------------------------------------------
-----             looks like our default perl libraries are not the conda ones. Manually setting perl5             -----
-----                                              library directory                                               -----
------------------------------------------------------------------------------------------------------------------------

metawrap path: /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/bin/metawrap
Will use perl5 libraries located in /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/perl5/site_perl/5.22.0 - hopefully they are there...

------------------------------------------------------------------------------------------------------------------------
-----                                       Starting binning with MaxBin2...                                       -----
------------------------------------------------------------------------------------------------------------------------

Can't locate HTTP/Status.pm in @INC (you may need to install the HTTP::Status module) (@INC contains: /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/perl5/site_perl/5.22.0/x86_64-linux-thread-multi /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/perl5/site_perl/5.22.0 /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2/x86_64-linux-thread-multi /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2 /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/5.26.2/x86_64-linux-thread-multi /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/5.26.2 .) at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2/LWP/Simple.pm line 14.
BEGIN failed--compilation aborted at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/site_perl/5.26.2/LWP/Simple.pm line 14.
Compilation failed in require at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/bin/run_MaxBin.pl line 4.
BEGIN failed--compilation aborted at /users/PAS1331/osu7930/miniconda3/envs/metawrap-env/bin/run_MaxBin.pl line 4.

************************************************************************************************************************
*****                              Something went wrong with running MaxBin2. Exiting.    

[ursky]

Try linking like this: export PERL5LIB=/users/PAS1331/osu7930/miniconda3/envs/metawrap-env/lib/perl5/site_perl/5.22.0 before trying to run maxbin again (you dont need to run metawrap to test it btw). Let me know if it works.

[morgvevans]

I tried including that in the bash script before the command but didn't see any difference in the output :/ I will skip using maxbin and just use metabat and concoct for now, I don't really need maxbin, unless this error will affect other processes down the line?

Thank you for your prompt assistance! Really grateful for this program.

[ursky]

No, it should only affect the binning and annotation modules.

[zhouyunyan]

Now I am having the same issue: Something went wrong with running MaxBin2. Exiting. I tried this: export PERL5LIB=/home/Pipeline/miniconda3/envs/metawrap-env/lib/perl5/site_perl/5.22.0, but it doesn't work.

[ursky]

I don't necessarily have specific advice because Perl libraries have odd properties on different systems. However, I would advise to solve the problem (MaxBin2 not working) rather than the symptom (metaWRAP failing). Look around and try to get the command run_MaxBin.pl -h to run on your system. The export PERL5LIB advice is how I was able to do it on my system.

[zhouyunyan]

I tried run_MaxBin.pl -h, The output is as follows:

$run_MaxBin.pl -h
readline() on closed filehandle FILE at /home/Pipeline/Software_NEW/miniconda3/envs/metawrap-env/bin/run_MaxBin.pl line 1336.
MaxBin 2.2.4
Unrecognized token [-h]
MaxBin - a metagenomics binning software.
Usage:
  run_MaxBin.pl
    -contig (contig file)
    -out (output file)

   (Input reads and abundance information)
    [-reads (reads file) -reads2 (readsfile) -reads3 (readsfile) -reads4 ... ]
    [-abund (abundance file) -abund2 (abundfile) -abund3 (abundfile) -abund4 ... ]

   (You can also input lists consisting of reads and abundance files)
    [-reads_list (list of reads files)]
    [-abund_list (list of abundance files)]

   (Other parameters)
    [-min_contig_length (minimum contig length. Default 1000)]
    [-max_iteration (maximum Expectation-Maximization algorithm iteration number. Default 50)]
    [-thread (thread num; default 1)]
    [-prob_threshold (probability threshold for EM final classification. Default 0.9)]
    [-plotmarker]
    [-markerset (marker gene sets, 107 (default) or 40.  See README for more information.)]

  (for debug purpose)
    [-version] [-v] (print version number)
    [-verbose]
    [-preserve_intermediate]

  Please specify either -reads or -abund information.
  You can input multiple reads and/or abundance files at the same time.
  Please read README file for more details.

[ursky]

Thats odd. What version of metawrap are you using, and how did you install it?

[zhouyunyan]

Version1.1.1, I installed it by conda.

[ursky]

That's probably your problem. Update!

[zhouyunyan]

is update metaWRAP to latest version?