bzhanglab / WebGestaltR

R package for WebGestalt
https://bzhanglab.github.io/WebGestaltR/
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`ERROR: No IDs are mapped` when Ensembl gene ids are provided as inputs #14

Closed integratebio closed 10 months ago

integratebio commented 2 years ago

Hello, I am using the WebGestaltRs 0.4.4 version on R 4.1.2. I got the ERROR: No IDs are mapped error when I provided Ensembl gene ids using interestGeneType="ensembl_gene_id" and referenceGeneType="ensembl_gene_id" arguments to the example code from the vignette i.e.

geneFile <- system.file("extdata", "interestingGenes.txt", package="WebGestaltR")
refFile <- system.file("extdata", "referenceGenes.txt", package="WebGestaltR")
outputDirectory <- getwd()
enrichResult <- WebGestaltR(enrichMethod="ORA", organism="hsapiens",
enrichDatabase="pathway_KEGG", interestGeneFile=geneFile,
interestGeneType="ensembl_gene_id", referenceGeneFile=refFile,
referenceGeneType="ensembl_gene_id", isOutput=TRUE,
outputDirectory=outputDirectory, projectName=NULL)

The full error message was as follows:

Loading the functional categories...
Loading the ID list...                                                                                             
ERROR: No IDs are mapped. Please check your input.Error in idMappingGene(organism = organism, dataType = dataType, inputGeneFile = inputGeneFile,  : 
  ERROR: No IDs are mapped. Please check your input.

For your reference, here are the input files I used: interestingGenes.txt referenceGenes.txt (protein coding genes) They are indeed Ensembl IDs. So I am not sure why I am getting this error.

Could you please let me know you advice on how I should be providing the Ensembl gene ids as input?

PS: I should note that interestingly, if I provide the same inputs i.e. Ensembl IDs with interestGeneType="genesymbol" and referenceGeneType="genesymbol" arguments, I don't get the error. But I am not sure if should trust the outputs of the enrichment analysis I got by this way. I would like if I could explicitly "tell" WebGestaltR that I am providing Ensembl IDs. I hope there is a way to do that.

yxngl commented 2 years ago

Hi, geneFile <- system.file("extdata", "interestingGenes.txt", package="WebGestaltR") get example data from the package. The files are in gene symbols. You should just use the file path to your input files.

On Sat, May 28, 2022 at 4:38 PM integratebio @.***> wrote:

Hello, I am using the WebGestaltRs 0.4.4 version on R 4.1.2. I got the ERROR: No IDs are mapped error when I provided Ensembl gene ids using interestGeneType="ensembl_gene_id" and referenceGeneType="ensembl_gene_id" arguments to the example code from the vignette i.e.

geneFile <- system.file("extdata", "interestingGenes.txt", package="WebGestaltR") refFile <- system.file("extdata", "referenceGenes.txt", package="WebGestaltR") outputDirectory <- getwd() enrichResult <- WebGestaltR(enrichMethod="ORA", organism="hsapiens", enrichDatabase="pathway_KEGG", interestGeneFile=geneFile, interestGeneType="ensembl_gene_id", referenceGeneFile=refFile, referenceGeneType="ensembl_gene_id", isOutput=TRUE, outputDirectory=outputDirectory, projectName=NULL)

The full error message was as follows:

Loading the functional categories... Loading the ID list... ERROR: No IDs are mapped. Please check your input.Error in idMappingGene(organism = organism, dataType = dataType, inputGeneFile = inputGeneFile, : ERROR: No IDs are mapped. Please check your input.

For your reference, here are the input files I used: interestingGenes.txt https://github.com/bzhanglab/WebGestaltR/files/8791891/interestingGenes.txt referenceGenes.txt https://github.com/bzhanglab/WebGestaltR/files/8791890/referenceGenes.txt (protein coding genes) They are indeed Ensembl IDs. So I am not sure why I am getting this error.

Could you please let me know you advice on how I should be providing the Ensembl gene ids as input?

PS: I should note that interestingly, if I provide the same inputs i.e. Ensembl IDs with interestGeneType="genesymbol" and referenceGeneType="genesymbol" arguments, I don't get the error. But I am not sure if should trust the outputs of the enrichment analysis I got by this way. I would like if I could explicitly "tell" WebGestaltR that I am providing Ensembl IDs. I hope there is a way to do that.

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