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caleblareau / mgatk

mgatk: mitochondrial genome analysis toolkit
http://caleblareau.github.io/mgatk
MIT License
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Failed to open file "CRA_test1_mgatk/temp/temp_bam/barcodes.8.temp0.bam" : No such file or directory #30

Closed bio-ruxandra-tesloianu closed 3 years ago

bio-ruxandra-tesloianu commented 3 years ago

Hi,

I am running mgatk tenx as advised in the documentation:

mgatk tenx -i $folder_bam/possorted_bam.bam -n CRA_test1 -o CRA_test1_mgatk -c 12 -bt CB -b $folder_bam/filtered_peak_bc_matrix/barcodes.tsv

I get the following error:

Error in checkGrep(grep(".A.txt", files)) : Improper folder specification; file missing / extra file present. See documentation Calls: importMito -> checkGrep Execution halted (myenv)jovyan@jupyter-bio-2druxandra-2dtesloianu:~$ [E::hts_open_format] Failed to open file "CRA_test1_mgatk/temp/temp_bam/barcodes.8.temp0.bam" : No such file or directory [Sat Nov 7 14:38:19 2020] Error in rule process_one_slice: jobid: 0 output: CRA_test1_mgatk/qc/depth/barcodes.8.depth.txt, CRA_test1_mgatk/temp/sparse_matrices/barcodes.8.A.txt, CRA_test1_mgatk/temp/sparse_matrices/barcodes.8.C.txt, CRA_test1_mgatk/temp/sparse_matrices/barcodes.8.G.txt, CRA_test1_mgatk/temp/sparse_matrices/barcodes.8.T.txt, CRA_test1_mgatk/temp/sparse_matrices/barcodes.8.coverage.txt

RuleException: SamtoolsError in line 93 of /home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx: 'samtools returned with error 1: stdout=, stderr=samtools sort: can\'t open "CRA_test1_mgatk/temp/temp_bam/barcodes.8.temp0.bam": No such file or directory\n' File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/snakemake/executors/init.py", line 2252, in run_wrapper File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx", line 93, in rule_process_one_slice File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/pysam/utils.py", line 75, in call File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/snakemake/executors/init.py", line 560, in _callback File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/concurrent/futures/thread.py", line 57, in run File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/snakemake/executors/init.py", line 546, in cached_or_run File "/home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/snakemake/executors/init__.py", line 2264, in run_wrapper Exiting because a job execution failed. Look above for error message [E::hts_open_format] Failed to open file "CRA_test1_mgatk/temp/temp_bam/barcodes.4.temp0.bam" : No such file or directory

When I do ls -lR on the CRA_test1_mgatk directory, it seems like it completely lacks the temp directory

(myenv)jovyan@jupyter-bio-2druxandra-2dtesloianu:~/CRA_test1_mgatk$ ls -lR .: total 12 drwxr-sr-x 2 jovyan users 4096 Nov 7 13:05 final drwxr-sr-x 4 jovyan users 4096 Nov 7 13:11 logs drwxr-sr-x 3 jovyan users 4096 Nov 7 14:38 qc

./final: total 120 -rw-r--r-- 1 jovyan users 121446 Nov 7 14:31 chrM_refAllele.txt

./logs: total 16 -rw-r--r-- 1 jovyan users 1392 Nov 7 14:37 base.mgatk.log -rw-r--r-- 1 jovyan users 602 Nov 7 14:37 CRA_test1.parameters.txt -rw-r--r-- 1 jovyan users 0 Nov 7 14:37 CRA_test1.snakemake_tenx.log drwxr-sr-x 2 jovyan users 4096 Nov 7 14:26 filterlogs drwxr-sr-x 2 jovyan users 4096 Nov 7 13:11 rmdupslogs

./logs/filterlogs: total 96 -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.10.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.11.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.12.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:25 barcodes.13.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.14.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.15.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.16.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.17.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:25 barcodes.18.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:25 barcodes.19.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.1.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:25 barcodes.20.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.21.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.22.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.23.filter.log -rw-r--r-- 1 jovyan users 24 Nov 7 14:26 barcodes.24.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.2.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.3.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.4.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.5.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.6.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.7.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.8.filter.log -rw-r--r-- 1 jovyan users 26 Nov 7 14:38 barcodes.9.filter.log

./logs/rmdupslogs: total 0

./qc: total 4 drwxr-sr-x 2 jovyan users 4096 Nov 7 13:11 quality

./qc/quality: total 0

caleblareau commented 3 years ago

hey sorry @bio-ruxandra-tesloianu somehow I missed seeing this-- did this get resolved somehow?

bio-ruxandra-tesloianu commented 3 years ago

hey sorry @bio-ruxandra-tesloianu somehow I missed seeing this-- did this get resolved somehow?

no :(

caleblareau commented 3 years ago

Could you cat the *logs/filterlogs* files?

we recently made some tweaks to tenx mode-- could you try running with version 0.6.0 (latest on Pypi) and let me know if anything gets better?

pk43429 commented 3 years ago

Hi @caleblareau,

I got the same error while running with version 0.6.1. Here is the output for cat the *logs/filterlogs*:

Kept 715654
Removed 5450
Kept 670028
Removed 5264
Kept 681359
Removed 5555
Kept 790015
Removed 6727
Kept 662046
Removed 6125
Kept 773335
Removed 6794
Kept 659653
Removed 5663
Kept 697615
Removed 6022
Kept 729339
Removed 6202
Kept 666933
Removed 5954
Kept 646504
Removed 5592
Kept 686152
caleblareau commented 3 years ago

And you're still getting an error associated with 

SamtoolsError in line 93 of /home/jovyan/my-conda-envs/myenv/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx:
'samtools returned with error 1: stdout=, stderr=samtools sort: can't open

?

It looks like the filtering is working as we would expect but the resulting bam file isn't being output... Can you try the same execution with the bcall mode and see if anything changes?

cnk113 commented 3 years ago

Hmm, so I also am running into this after installing mgatk (0.6.1) on a new system.

Error in rule process_one_slice:                                                                                                                                       
jobid: 0                                                                                                                                                                     
output: mgatk_out/qc/depth/barcodes.29.depth.txt, mgatk_out/temp/sparse_matrices/barcodes.29.A.txt, mgatk_out/temp/sparse_matrices/barcodes.29.C.txt, mgatk_out/temp/sparse_matrices/barcodes.29.G.txt, mgatk_out/temp/sparse_matrices/barcodes.29.T.txt, mgatk_out/temp/sparse_matrices/barcodes.29.coverage.txt                                                                                                                                                                                                                                                                                                                     
RuleException:                                                                                                                                                                                                   
OSError in line 113 of /home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx:                                                                                              
No such file or directory: 'mgatk_out/temp/ready_bam/barcodes.29.qc.bam'                                                                                                                                           
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/snakemake/executors/__init__.py", line 2352, in run_wrapper                                                                                   
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx", line 113, in __rule_process_one_slice                                                                        
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/pysam/utils.py", line 61, in __call__                                                                                                         
File "pysam/libcutils.pyx", line 293, in pysam.libcutils._pysam_dispatch                                                                                                                                      
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/snakemake/executors/__init__.py", line 569, in _callback                                                                                      
File "/home/chang/miniconda3/envs/venv/lib/python3.7/concurrent/futures/thread.py", line 57, in run                                                                                                              
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/snakemake/executors/__init__.py", line 555, in cached_or_run                                                                                  
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/snakemake/executors/__init__.py", line 2364, in run_wrapper

Run command

 mgatk tenx -i possorted_bam.bam -b filtered_peak_bc_matrix/barcodes.tsv -bt CB -o mgatk_out -c 48 --snake-stdout --keep-temp-files

I'm running bcall right now, but it's definitely taking much longer than usual.

cnk113 commented 3 years ago

Ok here is the bcall error:

Traceback (most recent call last):                                                                                                                                                                                 
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/mgatk/bin/python/oneSample.py", line 82, in <module>                                                                                            pysam.index(outputbam)                                                                                                                                                                                         
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/pysam/utils.py", line 61, in __call__                                                                                                      
save_stdout=kwargs.get("save_stdout", None))                                                                                                                                                                   
File "pysam/libcutils.pyx", line 293, in pysam.libcutils._pysam_dispatch                                                                                                                                       
OSError: No such file or directory: 'mgatk_out/temp/ready_bam/ACGCCACGTTGTGGTT-1.qc.bam'
MissingOutputException in line 21 of /home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/mgatk/bin/snake
/Snakefile.Scatter:                                                                             
Job Missing files after 5 seconds:                                                                                                                                                                               
mgatk_out/temp/ready_bam/ACGCCACGTTGTGGTT-1.qc.bam                                                                                                                                                               
mgatk_out/temp/ready_bam/ACGCCACGTTGTGGTT-1.qc.bam.bai
mgatk_out/qc/depth/ACGCCACGTTGTGGTT-1.depth.txt                                                                                                                                                                  
mgatk_out/temp/sparse_matrices/ACGCCACGTTGTGGTT-1.A.txt
mgatk_out/temp/sparse_matrices/ACGCCACGTTGTGGTT-1.C.txt                                                                                                                                                          
mgatk_out/temp/sparse_matrices/ACGCCACGTTGTGGTT-1.G.txt                                                                                                                                                          
mgatk_out/temp/sparse_matrices/ACGCCACGTTGTGGTT-1.T.txt                                                                                                                                                          
mgatk_out/temp/sparse_matrices/ACGCCACGTTGTGGTT-1.coverage.txt                                                                                                                                                   
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.                                                                                            
Job id: 0 completed successfully, but some output files are missing. 0                                                                                                                                            
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/snakemake/executors/__init__.py", line 584, in handle_job_success                                                                             
File "/home/chang/miniconda3/envs/venv/lib/python3.7/site-packages/snakemake/executors/__init__.py", line 259, in handle_job_success                                                                           
Exiting because a job execution failed. Look above for error message

Seems like there was similar issue earlier, but I don't think it's the same in regards to the duplicate marking step. I have Java 1.8 installed.

caleblareau commented 3 years ago

So bcall will take much longer in general than tenx mode. If you want to trouble shoot, just supply a shorter list of barcodes (e.g. head filtered_peak_bc_matrix/barcodes.tsv > short.tsv; then supply -b short.tsv

Anywho, I'm not sure that I see a consistent error here compared to what it previously was. You verified java, which is great. Can you send ls -lR of the mgatk output folder?

cnk113 commented 3 years ago

Log.txt Here's the output dir.

caleblareau commented 3 years ago

just to verify @cnk113 did the pipeline work on the test data? It looks liek the per-sample processing just stopped randomly at some point in the cell processing, which is extremely unusual. I don't think that I've seen this behavior before...

cnk113 commented 3 years ago

Oops, that was my old one (I also forcefully killed it once it started failing), here's the one with a few bcs (this I let it run until it fizzled out). Log.txt

bio-ruxandra-tesloianu commented 3 years ago

I am quite late here but my error was solved by doing snake stdout at the end of the command

cnk113 commented 3 years ago

I ran it with snake stdout. I've previously solved an earlier issue like that. This is new.

cnk113 commented 3 years ago

So I've been trying to debug this, and tried this on multiple different Ubuntu versions and python version/env. From what I understand, everything is fine until filterClipBam which ends up not printing the bam file (I can see it run in htop but almost instantaneously fails). This seems to be an os.system() failure or probably one of the parameters being the issue?

cnk113 commented 3 years ago

Ok I found the issue:

Exception in thread "main" picard.PicardException: This program requires input that are either coordinate or query sorted (according to the header, or at least ASSUME_SORT_ORDER and the content.) 
Found ASSUME_SORT_ORDER=null and header sortorder=unsorted                                                                                                                                                                            
at picard.sam.markduplicates.MarkDuplicates.doWork(MarkDuplicates.java:294)                                                                                                                                      
at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:295)                                                                                                                                   
at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:103)                                                                                                                                     
at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:113)

Pretty much it seems the header showed unsorted even though it was sorted through pysam sort. I just added ASSUME_SORTED=true to your picard command and it works.

However I now get a different error towards the end (almost there!)

Traceback (most recent call last):                                                                                                                                                                                 
File "/media/chang/HDD-6/chang/mg/atac/Adult_DSP_MG_multi_atac_cr/outs/mgatk/mgatk/bin/python/variant_calling.py", line 88, in <module> base_coverage_dict = load_mgatk_output(MGATK_OUT_DIR)
File "/media/chang/HDD-6/chang/mg/atac/Adult_DSP_MG_multi_atac_cr/outs/mgatk/mgatk/bin/python/variant_calling.py", line 30, in load_mgatk_output fwd_base_df[missing_pos] = 0  # fill in missing positions                                                                                                                                                    
File "/home/chang/miniconda3/envs/venv3.6/lib/python3.6/site-packages/pandas/core/frame.py", line 2935, in __setitem__                                                                                             self._setitem_array(key, value)                                                                                                                                                                               
File "/home/chang/miniconda3/envs/venv3.6/lib/python3.6/site-packages/pandas/core/frame.py", line 2966, in _setitem_array                                                                                          key, axis=1, raise_missing=False
File "/home/chang/miniconda3/envs/venv3.6/lib/python3.6/site-packages/pandas/core/indexing.py", line 1553, in _get_listlike_indexer keyarr, indexer, o._get_axis_number(axis), raise_missing=raise_missing                                                                                                                                         
File "/home/chang/miniconda3/envs/venv3.6/lib/python3.6/site-packages/pandas/core/indexing.py", line 1640, in _validate_read_indexer raise KeyError(f"None of [{key}] are in the [{axis_name}]")                                                                                                                                                  
KeyError: "None of [Int64Index([    1,     3,     4,     6,     8,     9,    10,    11,    12,\n               14,\n            ...\n            16551, 16555, 16558, 16560, 16562, 16563, 16565, 16566, 16568,\n            16569],\n           dtype='int64', length=11090)] are in the [columns]"                                                                                                                              
MissingOutputException in line 163 of /media/chang/HDD-6/chang/mg/atac/Adult_DSP_MG_multi_atac_cr/outs/mgatk/mgatk/bin/snake/Snakefile.tenx:                                                                    
Job Missing files after 5 seconds:                                                                                                                                                                               
mgatk_out/final/mgatk.variant_stats.tsv.gz                                                                                                                                                                       
mgatk_out/final/mgatk.cell_heteroplasmic_df.tsv.gz                                                                                                                                                               
mgatk_out/final/mgatk.vmr_strand_plot.png                                                                                                                                                                        
This might be due to filesystem latency. If that is the case, consider to increase the wait time with --latency-wait.                                                                                            
Job id: 0 completed successfully, but some output files are missing. 0                                                                                                                                             
File "/home/chang/miniconda3/envs/venv3.6/lib/python3.6/site-packages/snakemake/executors/__init__.py", line 589, in handle_job_success                                                                          
File "/home/chang/miniconda3/envs/venv3.6/lib/python3.6/site-packages/snakemake/executors/__init__.py", line 259, in handle_job_success

2021-05-28T214711.286892.snakemake.log

cnk113 commented 3 years ago

Ok so the latest error seems to be sample specific, I'm assuming no variant was confidently detected or there wasn't enough MT coverage.

rpolicastro commented 3 years ago

I just wanted to add that I get the same error with failing to find the bam file.

caleblareau commented 3 years ago

Implemented this fix in v0.6.3-- hopefully this solves things, but please comment on thread if not!

mvinyard commented 2 years ago

Hi @caleblareau - thanks for making this great tool. I am encountering the same issue using v0.6.4 where the files in mgatk_out/temp/ seem to be called after they are deleted. I read through this thread but the implemented solution does not seem to carry over for me - please excuse me if I am missing a key detail.

Using:

I've included all of the outputs of mgatk below - I am happy to supply any other potentially useful information.

Command: 

mgatk tenx --input outs/possorted_bam.bam -bt CB -b outs/filtered_peak_bc_matrix/mini.tsv --mito-genome hg38 --ncores=14

Error message

```BASH [Thu Dec 23 21:22:31 2021] Error in rule process_one_slice: jobid: 0 output: mgatk_out/qc/depth/barcodes.8.depth.txt, mgatk_out/temp/sparse_matrices/barcodes.8.A.txt, mgatk_out/temp/sparse_matrices/barcodes.8.C.txt, mgatk_out/temp/sparse_matrices/barcodes.8.G.txt, mgatk_out/temp/sparse_matrices/barcodes.8.T.txt, mgatk_out/temp/sparse_matrices/barcodes.8.coverage.txt RuleException: OSError in line 113 of /home/mvinyard/.anaconda3/envs/mgatk/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx: No such file or directory: 'mgatk_out/temp/ready_bam/barcodes.8.qc.bam' File "/home/mvinyard/.anaconda3/envs/mgatk/lib/python3.7/site-packages/mgatk/bin/snake/Snakefile.tenx", line 113, in __rule_process_one_slice File "/home/mvinyard/.anaconda3/envs/mgatk/lib/python3.7/site-packages/pysam/utils.py", line 61, in __call__ File "pysam/libcutils.pyx", line 323, in pysam.libcutils._pysam_dispatch File "/home/mvinyard/.anaconda3/envs/mgatk/lib/python3.7/concurrent/futures/thread.py", line 57, in run Exiting because a job execution failed. Look above for error message Shutting down, this might take some time. Exiting because a job execution failed. Look above for error message Complete log: /home/mvinyard/data/test_sample/.snakemake/log/2021-12-23T212219.494102.snakemake.log ```

find ./mgatk_out/ produces the following file structure:

``` mgatk_out mgatk_out/final mgatk_out/final/chrM_refAllele.txt mgatk_out/qc mgatk_out/qc/quality mgatk_out/logs mgatk_out/logs/mgatk.parameters.txt mgatk_out/logs/rmdupslogs mgatk_out/logs/base.mgatk.log mgatk_out/logs/mgatk.snakemake_tenx.log mgatk_out/logs/filterlogs mgatk_out/logs/filterlogs/barcodes.5.filter.log mgatk_out/logs/filterlogs/barcodes.7.filter.log mgatk_out/logs/filterlogs/barcodes.6.filter.log mgatk_out/logs/filterlogs/barcodes.3.filter.log mgatk_out/logs/filterlogs/barcodes.4.filter.log mgatk_out/logs/filterlogs/barcodes.2.filter.log mgatk_out/logs/filterlogs/barcodes.10.filter.log mgatk_out/logs/filterlogs/barcodes.8.filter.log mgatk_out/logs/filterlogs/barcodes.1.filter.log mgatk_out/logs/filterlogs/barcodes.9.filter.log ```

cat base.mgatk.log produces:

``` Thu Dec 23 21:22:17 UTC 2021: Starting analysis with mgatk Thu Dec 23 21:22:17 UTC 2021: Processing samples with 14 threads Thu Dec 23 21:22:17 UTC 2021: mgatk successfully processed the supplied .bam files Thu Dec 23 21:22:26 UTC 2021: Successfully created final output files Thu Dec 23 21:22:26 UTC 2021: Intermediate files successfully removed. ```

*` cat filterlogs/`** produces:

``` Kept 35386 Removed 25 Kept 23104 Removed 11 Kept 51542 Removed 19 Kept 42683 Removed 39 Kept 86645 Removed 37 Kept 18473 Removed 11 Kept 211905 Removed 136 Kept 66058 Removed 38 Kept 90319 Removed 53 Kept 33776 Removed 31 ```

cat mgatk.parameters.txt produces:

``` input_directory: 'outs/possorted_bam.bam' output_directory: 'mgatk_out' script_dir: '/home/mvinyard/.anaconda3/envs/mgatk/lib/python3.7/site-packages/mgatk' fasta_file: '/home/mvinyard/.anaconda3/envs/mgatk/lib/python3.7/site-packages/mgatk/bin/anno/fasta/hg38.fasta' mito_chr: 'chrM' mito_length: '16569' name: 'mgatk' base_qual: '0' remove_duplicates: 'True' handle_overlap: 'False' low_coverage_threshold: '10' barcode_tag: 'CB' umi_barcode: 'MU' alignment_quality: '0' emit_base_qualities: 'False' proper_paired: 'False' NHmax: '1' NMmax: '4' max_javamem: '8000m' ```

cat mgatk.snakemake_tenx.log produces nothing.

Probably unnecessary but just in case, cat mgatk_out/final/chrM_refAllele.txt | head -10 produces:

``` 1 G 2 A 3 T 4 C 5 A 6 C 7 A 8 G 9 G 10 T ```

I think that's everything - I will keep trouble-shooting in the meantime and provide an update if I find a solution. Thanks in advance for your time and any potential help!

caleblareau commented 2 years ago

Can you try with --snake-stdout ?

mvinyard commented 2 years ago

Can you try with --snake-stdout ?

Ah this seems to clear up the issue.

I saw it mentioned above

I ran it with snake stdout. I've previously solved an earlier issue like that. This is new.

but thought this would be a separate case. I'm not sure why the snakemake workflow requires that but it's enough for me. I am currently testing the outputs in downstream workflows. Thanks for your help, Caleb!

caleblareau commented 2 years ago

I have no idea either but glad it's working now :)