Closed chrismahony closed 2 years ago
I am running the following:
mgatk tenx -i /mypath//atac_possorted_bam.bam \ -n Pilot1 -o Pitlo1_mgatk -c 12 \ -bt CB -b /mypath/outs/barcodes.tsv
and I get the following output:
Found file of barcodes to be parsed: /mypath/outs/barcodes.tsv Mon Jan 10 11:34:57 GMT 2022: User specified mitochondrial genome does NOT match .bam file; correctly specify reference genome or .fasta file
Any ideas? Many thanks in advance Chris
OK I solved this by adding the --mito-genome mm10 flag, it appears to have worked
I am running the following:
mgatk tenx -i /mypath//atac_possorted_bam.bam \ -n Pilot1 -o Pitlo1_mgatk -c 12 \ -bt CB -b /mypath/outs/barcodes.tsv
and I get the following output:
Found file of barcodes to be parsed: /mypath/outs/barcodes.tsv Mon Jan 10 11:34:57 GMT 2022: User specified mitochondrial genome does NOT match .bam file; correctly specify reference genome or .fasta file
Any ideas? Many thanks in advance Chris