search

calquezar / OpenCASA

GNU General Public License v3.0
26 stars 10 forks source link

About NAN. I had tried many times, but still get NAN! #12

Open kimjinou opened 2 months ago

kimjinou commented 2 months ago

I am conducting tests on the vitality of the swimming spores of kelp. I meet a few questions. Firts, unsupported compressed. I use Virtualdub save my videos to uncompressed avi, problem sloved. Second, The NAN ! I tried to change the parameteres, but it didn't work. Can someboby help me? Here is the videos parameteres: Under 4X ； 30882064；30fps; 0.8microns per pixel(not sure ,because it was derivation by 19201080's 2.089 microns per pixel). 屏幕截图 2024-09-09 114152 Here is the video:https://drive.google.com/file/d/1MNkb1uZ-UYKzcmGTt_MajRfjaAhAyoYX/view?usp=sharing

Here is a processed videos ：（it cloud used in casa but i found it may not right):https://drive.google.com/file/d/1ZdWhqyNAUA-mK8-5Hz2TfycCF7SpiN4D/view?usp=sharing

Hope you could give me some advices. Sincere thanks！

kimjinou commented 2 months ago

New thing to add! I tried again with a 10X videos. I found fellow this parameters could get the results. But I think it might be wrong， because these tracks looks like not belong to sperm. And many sperm didn't get tracks. 屏幕截图 2024-09-09 142136

屏幕截图 2024-09-09 141920 Here is introduction about what am i doing. We want to combine the motility and viability(how many kelp's sperms can move) to test the diffrent kelp fertility to guide the proudct. So we really want to know how to use Opencasa well. Sincere thanks!

                    [ Here is the 10x videos:  https://drive.google.com/file/d/1j7G4XfrwR-o0o-FWqpLNB5MIkuYTwBVq/view?usp=drive_link

here is another do therload videos: https://drive.google.com/file/d/17Oo59OD-hi_aiAGHJkMHfNpCpnW_Slxz/view?usp=sharing