Method used to make the pseudoreplicates

cancerbits / DElegate

Wrapper and helper functions to use bulk RNA-seq differential expression methods with single-cell data

GNU General Public License v3.0

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Method used to make the pseudoreplicates #3

Open LettAdam opened 2 months ago

LettAdam commented 2 months ago

Hello,

I don't know if I missed it in the article or in the vignette but I'm wondering exactly how the pseudo replicates are made, in particular, when using a seurat object.

Thank you for your work.

ChristophH commented 2 months ago

Hi, If no replicates are specified in the input, the cells in every group (cluster) are randomly split into sub-clusters (pseudo-replicates, by default 3 per group). The counts (from the "RNA" slot) are then summed per pseudo-replicate.