fa8sanger
commented
1 day ago Thank you! We are aware of that problem, and there’s a branch (hotfix/3.5.8) fixing it. And if you are brave to try untested versions, we also have this forked repo with some recent fixes (https://github.com/fa8sanger/NanoSeq/tree/develop)
Regarding your creative solution, that should work perfectly, no problem at all.
On 22 Nov 2024, at 09:08, toshwatatosh @.***> wrote:
We are now working on Nanoseq in a monkey. Human and mouse chromosomes usually start with N. But, in this monkey, some chromosomes start with ATGC. When sequence reads are mapped to the end of such chromosomes, errors happen at the dsa step. To avoid this problem, we remove reads at the ends of the chromosome. Could this removal affect the final mutation burden rate or other results?
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We are now working on Nanoseq in a monkey. Human and mouse chromosomes usually start with N. But, in this monkey, some chromosomes start with ATGC. When sequence reads are mapped to the end of such chromosomes, errors happen at the dsa step. To avoid this problem, we remove reads at the ends of the chromosome. Could this removal affect the final mutation burden rate or other results?