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cancerit / alleleCount

Support code for NGS copy number algorithms. Takes a file of locations and a [cr|b]am file and generates a count of coverage of each allele [ACGT] at that location (given any filter settings)
http://cancerit.github.io/alleleCount/
GNU Affero General Public License v3.0
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Unpaired data - No alleleCount when reads available #72

Closed ShixiangWang closed 7 months ago

ShixiangWang commented 2 years ago

Hi developers,

Thanks for developing this tool. I have questions when I use this tool. I found I cannot count any allele in a bam file, even with -m 0 -q 0. However, I found the bam file has nothing wrong. Below is an example.

Why this happen? Any suggestions?

Best,

Shixiang

$ alleleCounter -l test.loc -b ~/data/gdc/wes/TCGA-AA-3869-10A-01W-0995-10_hg19_Illumina_gdc_realn.bam -o test.txt -m 0 -q 0

Reading locis
Done reading locis

$ cat test.txt 
#CHR    POS Count_A Count_C Count_G Count_T Good_depth
chr3    58125556    0   0   0   0   0
chr3    58125557    0   0   0   0   0
chr3    58125558    0   0   0   0   0
chr3    58125559    0   0   0   0   0
chr3    58125560    0   0   0   0   0

$ samtools view -b ~/data/gdc/wes/TCGA-AA-3869-10A-01W-0995-10_hg19_Illumina_gdc_realn.bam chr3:58125556-58125560 | samtools view -
[W::hts_idx_load3] The index file is older than the data file: /public/home/zhaoqi/data/gdc/wes/TCGA-AA-3869-10A-01W-0995-10_hg19_Illumina_gdc_realn.bai
HWI-ST115_0148:1:1:2828:131031#0    0   chr3    58125457    60  100M    *   0   0   TTTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGC    46659B=BA?B@DB?@?>B>@?DB?@EA<ACC@7ABA?C>AAAAABC@CAB@AC?@??@@AC@@DBCCCC@D?DC@A>?=>=ABCBA@=B?<>=@@;<;8    BD:Z:JJAKPPLCMLJKHLKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMNJJLMKAKJMLOOOLCMLLNN   MD:Z:100    RG:Z:0.1    BI:Z:NNHOQROHPPNOMQPQQPOOPQOPQPOQNOPNOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJKQQPIPORQRRQNGNPPQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:66:8947:69599#0    1024    chr3    58125457    60  100M    *   0   0   TTTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGC    46659@=AA>B@DB>@@>B?@?DB??D@A@CBA8@B?>C>@@B?@BB@CAC?@B@A?@A@?@<<C@BBBBBE@DBCB>@><>ABBB?A?B??DBA@9=:8    BD:Z:IIAJPOLBLMJKIMKLMKKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLJJNLLKLKJKMKMJAAAJKHIIAKLMMJJKLJAKJMMOPOKCLLLNN   MD:Z:100    RG:Z:0  BI:Z:MMGOQRPGPONNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLOGQPQPKKQQOHPNQQRRPNGNOPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:61:8962:119547#0   16  chr3    58125457    60  100M    *   0   0   TTTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGA    ><<:>CCC?CA?B@CB?C?C@@BF?AD@AA<A8BCE<A?EACEBCCAABADABCA>CACABB@?BCCCCA@>?CCB>CAA@>ABCD@>BD@>A@?77844    BD:Z:BKLMLBMMKLJNLLLMLLLNNMLMMMJMMJJMKMLMNMKJMLKJ@JMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKMNNLBMOOMLJJ   MD:Z:99C0   RG:Z:0.1    BI:Z:HOPOMGOQQOORQQQQQQQOQQQPQPOQQPOQOQQPRQPOQQPOHNQPOQPONPPPONOOOPQQPHHHOQLMOGNPOOJJNPOHNMPPQPQOHPQRQPNN   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:5:15563:60243#0    0   chr3    58125458    60  100M    *   0   0   TTCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCG    3533=?@A>A?DB?@?>B>@?DB?=D@A@CBA7@A??C>@@B?@BB@CAC?AB@@?@A@@CB@DBBCBBBDADBBC>@>>>ABBB?@????CBB@<=:92    BD:Z:IIJLPLDLMKKIMLLMKKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIJNLMJLKJKMKMJAAAJKHIIAKLMMJJKLJAKIMMOOOKCMMLNNK   MD:Z:100    RG:Z:0  BI:Z:MMOORPIPONNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNHPPQPKKQQOHPNQQQRQOGPMPPPM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:22:21121:84028#0   0   chr3    58125459    60  100M    *   0   0   TCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGT    3329>??=B?DB??@?B>@?EA@?C>A@CC@7>BA?C>@AB?ACC>C?D@AC@A??A?@BB@DBCCCC@D@BBCB?A>>>A@CC@A?BA?B5@:.@=92;    BD:Z:JJMMMENMKKIMKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIJLMKAKJMLNNOKCNMMNNLM   MD:Z:100    RG:Z:0.1    BI:Z:NNOQPIQPNOMQPQQQPOPQOPPPPPNPOMOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPQPIPORQRRQOIPONQQNQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:24:18332:73108#0   1024    chr3    58125459    60  100M    *   0   0   TCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGT    4548>>@=A@DB>@@>B>@?DB??D@A@CBA7@B??C>@@B?@CB@CAC?BBAA?@A@ACB@EBCCCCBE@ECCB@B>>>BBCCA@?C@@ECCB>A<92;    BD:Z:JJMMMENMKKIMKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIJLMKAKJMLNNOKCNMMNNLM   MD:Z:100    RG:Z:0.1    BI:Z:NNOQPIQPNOMQPQQQPOPQOPPPPPNPOMOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPQPIPORQRRQOIPONQQNQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:67:17292:45690#0   1024    chr3    58125459    60  100M    *   0   0   TCCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGT    332:<??=>=EB?A@>????EB?@CAAADC@7?B??C>???AACA@C@D?AAAA??A?ACB@EBCCCC?C@CABB?????<:BB@A?@@?DCCA>@>92;    BD:Z:JJMMMENMKKIMKMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIJLMKAKJMLNNOKCNMMNNLM   MD:Z:100    RG:Z:0.1    BI:Z:NNOQPIQPNOMQPQQQPOPQOPPPPPNPOMOOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPQPIPORQRRQOIPONQQNQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:22:13156:141937#0  0   chr3    58125460    60  100M    *   0   0   CCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTT    3388=><A?DA>>?>A>?>DA@?D>A@CC@7ABA>C?@ABAAAC@A@C>AB@@??B?ACB@DBC@CC@E@DCAB???5<@BCC@A?BA?B@BB?B>;0<=    BD:Z:JJMJEONKLIMLMMLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIILMKAKJMLNNNKCMNMONLMK   MD:Z:100    RG:Z:0.1    BI:Z:NNQNJQQNOMQPQQQPPPQOPPPOQNOPMNOQPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPPIPORQRRQOIQPOOQNQN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:1:15839:10992#0    1024    chr3    58125460    60  100M    *   0   0   CCAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAGGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTT    4488;><A?DB>@@>B>@?DB??D@@@CBA7@B??C>@@B?@BB@CAC?@@>@>@B@@CB@DABBBCADAECBC>A>>>ABBB?@>B@?DBBB>A=:29;    BD:Z:IILIENOKLJMLMNKKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLKLKLMKLJJKMKMJAAAJKHIIAKLMMJJKLJAKILLOONJCMNNONKLJ   MD:Z:50A49  RG:Z:0  BI:Z:MMPNIRQNNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPOOOPPOOONPPPPNGGGOPLLNGPPPPKKQQOHPNQQQQPPIQOPNPMOM   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:44:12431:82847#0   0   chr3    58125461    60  100M    *   0   0   CAAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTT    4756;:@?CB>@@>B>@?DB??D@@@CBA7AB??C>@@B?@BB@CAC?@B@@>@@@ABB@DBBBBBBD@DBCC?@?>>ABBB?@>B?@DBBB>B>=29<=    BD:Z:IIIAOOMLJNLMNLKKLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKMKLKIKMKMJAAAJKHIIAKLMMJJKLJAKILLNONJBMNNPOKLJA   MD:Z:100    RG:Z:0  BI:Z:MMNGRQPNLPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOQOKKQQOHPNQQQQPOIRPPPNMOMG   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:66:3322:117270#0   0   chr3    58125462    60  100M    *   0   0   AAATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTC    36667>>CA?@?>B>@>DB??D@@@CBA7ABA?C>@@B?@CC@CAC?AC@A?@A@BCB@DBCCCC@DADBCC??>>>ABCCAA?B@@BCCB?BA?6==>=    BD:Z:JJBLOMMJNLNNLKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLKAKJMLNNNJBMMMPOMMKAK   MD:Z:100    RG:Z:0.1    BI:Z:NNHPROPMQPQQQPPQROPPPOPNPOMONPPQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHPORQRRQOIQQQQPLQNHO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:43:4574:152452#0   0   chr3    58125463    60  100M    *   0   0   TATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT    4252;<BA>@?>B>@>DB??A@A@CB@7@BA?C>@@B?@BB@CAC?@C@A?@AAACC@DACCCB@EAECCC?A=>>ABDC@@?B@@FCCB?BA?7?>=<>    BD:Z:JJLLMNKNMNNMKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJAKJMLNNNJBLMMOPMNKAKK   MD:Z:0A99   RG:Z:0.1    BI:Z:NNPPPPNQPQQQPPQRPPPPOPNOPMNOPOQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHOORQRRQOIQQQRQMONHOO   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:64:16055:12934#0   1024    chr3    58125463    60  100M    *   0   0   TATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT    4262;<BA=@@>B>@?DB??E@@@CBA7AB??C>@@B?@CB@CAC?AB@@??A?ABB@DBCCCC@EADCCC?A???@CCC@@?C@@ECCC?C@?7?>><?    BD:Z:JJLLMNKNMNNMKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJAKJMLNNNJBLMMOPMNKAKK   MD:Z:0A99   RG:Z:0.1    BI:Z:NNPPPPNQPQQQPPQRPPPPOPNOPMNOPOQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHOORQRRQOIQQQRQMONHOO   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:64:5368:84525#0    1024    chr3    58125463    60  100M    *   0   0   AATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT    4754=<B@>?@>A>@>DBA?E@B@CCA7AB??C??AB@ACC@C@CAAC?A?@@?ACB?CBCCCC@D@DCBB???=?ABCC@A?BA?DBBB?B@=7@?=;>    BD:Z:JJLLMNKNMNNMKKMNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJAKJMLNNNJBLMMOPMNKAKK   MD:Z:100    RG:Z:0.1    BI:Z:NNPPPPNQPQQQPPQRPPPPOPNOPMNOPOQQOPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHOORQRRQOIQQQRQMONHOO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:3676:113118#0   1024    chr3    58125463    60  100M    *   0   0   AATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCT    3554<;@?=>=>=:=9>679A:=9=76'8.?<6;<;7.37A:A??AAA?@>?:@BA=>B@@@8=?D@ABB==<9:>AA=B;??;?=ABA>>@??4>=:99    BD:Z:IIKLNNLNMNNLLLLNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJLKJKLKMJAAAJKHIIAKLMMJJKLJAKILLNNMJBLMNPPMMJAJJ   MD:Z:100    RG:Z:0  BI:Z:MMPOPPNPOPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOKJQQOHPNQQQQPOHQQRQPMMMGON   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:22:7204:119714#0   0   chr3    58125464    60  100M    *   0   0   ATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTG    46269@@>?@>B>@?BB??D@@@CBA8@A??C?@@B@@BB@@AC?AB@@>?A??BB>C?B@BB@C@EBBC?A??>ABBB?@>B??CBCB?B??7?@=<<:    BD:Z:IILJOLPMNOLLLMNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKKJKMJMJAAAJKHIIAKLMMJJKLJAKILLNNMIBLMMPPMNKAJJM   MD:Z:100    RG:Z:0  BI:Z:MMONPNROPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJKPQOHPNQQQQPOHQPRRQMOKGONP   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:27:5413:149006#0   1024    chr3    58125464    60  100M    *   0   0   TTAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTG    4526:@@>??>B???BA?:D@@?CBA8AB@?C=?@B?@BB@CAC?BB?@>@A@ABB@DB@BB@@C@DBBB?@?>>ABBB?@>AA?CBBB?B??7@@=:<8    BD:Z:IIJJOLPMNOLLLMNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKKJKMJMJAAAJKHIIAKLMMJJKLJAKILLNNMIBLMMPPMNKAJJM   MD:Z:0A99   RG:Z:0  BI:Z:MMPNPNROPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJKPQOHPNQQQQPOHQPRRQMOKGONP   NM:i:1  AS:i:99 XS:i:20
HWI-ST142_0240:1:3:16511:17168#0    1024    chr3    58125464    60  100M    *   0   0   ATAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAACTAGGGGGTTTGTATGCACATATGCGGTTCTG    20396418;<=A>>=C?@>C>@>@@?79A>6@;=:>@>?>>?>A>BB>?9:2>??68B7?BBB?D%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:IILJOLPMNOLLLMNLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKKJKMJMJAAAJKHIIKLLMMJJJLJAKILLNNMHJNMMPPMNMJJJM   MD:Z:68A6A10A7T5    RG:Z:0  BI:Z:MMONPNROPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNNPPPOJKJPNHPNQQQQPMMQPRRQMMMMONP   NM:i:4  AS:i:80 XS:i:20
HWI-ST142_0240:1:26:3068:140930#0   0   chr3    58125465    60  100M    *   0   0   TAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGT    4264>><?>>A?@?DB??D@@>CBA7@A??C?@@B@@BB@CAC?@B@@>@A@@BB@DBBBBBBD@DCBB?@>>?BBBB@@>B??CCBB?B??7@A@<=:;    BD:Z:IIJKMPONOMLLMOLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJJKMKLJAAAJKHIIAKLMMJJKLJAKILLNNMIALMMOPMNLBJJMI   MD:Z:100    RG:Z:0  BI:Z:MMNNNRQPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJQPOHPNQQQQPOHQPQRRNOMEONPM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:65:3969:160251#0   1024    chr3    58125465    60  100M    *   0   0   TAACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGT    4264=><??>B>@=D??>C@@@CBA7@>??C>@@B?@BB@CAC?AB@?>@A?@BA@D@BBBB@D@DBBB?A???AABB?@>B@?DBBC>B?>7@A@<<:;    BD:Z:IIJKMPONOMLLMOLLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJJKMKLJAAAJKHIIAKLMMJJKLJAKILLNNMIALMMOPMNLBJJMI   MD:Z:100    RG:Z:0  BI:Z:MMNNNRQPQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJQPOHPNQQQQPOHQPQRRNOMEONPM   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:21:18981:131709#0  0   chr3    58125466    60  100M    *   0   0   AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT    3649<<?>>B>?>DB?@E>AADAA7AB@?C?@ABAACCAC@D?AA@@??A?BCB@EBC@CC@B@DCBB??>>=@BCD@A?BA?ECBB?B@?5ACC?@<<;    BD:Z:JJKIPOPONLLNNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JIMLNNNJBLLLNOMNMBLKNJK   MD:Z:100    RG:Z:0.1    BI:Z:NNOMSQRQQPPQRPQQQOPNOOMNOPOQPNPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPRRQOIQQQRRORNGMOPNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:22:5666:176436#0   1024    chr3    58125466    60  100M    *   0   0   AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT    373;<;<>=A<><A??<B?>@BA@7?>>>A;?AA@ACC@A@C@;A@A?@@=@???DBB?B@>B?BCC@?>=>><@>9:88@A?DB@B??@>6=@@>?:<;    BD:Z:JJKIPOPONLLNNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JIMLNNNJBLLLNOMNMBLKNJK   MD:Z:100    RG:Z:0.1    BI:Z:NNOMSQRQQPPQRPQQQOPNOOMNOPOQPNPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPRRQOIQQQRRORNGMOPNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:5:17906:70621#0    1024    chr3    58125466    60  100M    *   0   0   AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT    373:<;=>>@>?>EB@>C>@@CC@7ABA?C?@ABAACC@C@C@BC?A?@=;=>?;DBCBCC@D@ACCB????>ABCCA@?B@?DCC@?BA?7@CC??<;=    BD:Z:JJKIPOPONLLNNMLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JIMLNNNJBLLLNOMNMBLKNJK   MD:Z:100    RG:Z:0.1    BI:Z:NNOMSQRQQPPQRPQQQOPNOOMNOPOQPNPQOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPRRQOIQQQRRORNGMOPNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:62:3161:55853#0    1024    chr3    58125466    60  100M    *   0   0   AACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTT    4648;:>?=B>@?DB??D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@@@BBA@DBBBBBAD@DCBB>A>>?AABB?@?B??CABB?B=?8@BA?>:;=    BD:Z:IIKIQOPOMMLMOMLMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIKMKMIAAAJKHIIAKLMMJJKLJAKILLNNMIAKMMOOMNLCKJMIJ   MD:Z:100    RG:Z:0  BI:Z:MMNLRQRQPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPQNHPNQQQQPOHQPQQROPMGMNPMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:66:7395:110588#0   0   chr3    58125467    60  100M    *   0   0   ACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTT    44867<>=A>@?DB??D@@@CBA7@B??C>@AB?@BB@CAC?@B@@?@@@ABB@DBBBBBADADBBC>@?>?BBBC?@>B;?DBBB>C@?7@BB@A:;<=    BD:Z:IIIMPPQMMMMOMMMMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJMKMJ@AAJKHIIAKLMMJJKLJAKILLNNMIAKLMOOLNLCLKMIJA   MD:Z:100    RG:Z:0  BI:Z:MMLPQRSPPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPOGPNQQQQPOHQPQQQOQNGOLPMMG   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:63:9558:92531#0    16  chr3    58125467    60  100M    *   0   0   ACATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTT    ;:<9A?>B>B??CD??CA@@B@7@CD@@?DBBDBBB@A@@DABC@>CACABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@AA><=4654    BD:Z:INLLMNNNNNOONMNMJMNJJMKMMMOMLJMMKJAJNLKNKJHLLLJIIIIKLMJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKMMMKLBKNQMKAII   MD:Z:100    RG:Z:0  BI:Z:MPPPNOOQRPQQQPQQOQRPOQOQPPQPPNPPPOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNQRNNGMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:45:1957:27774#0    0   chr3    58125468    60  100M    *   0   0   TATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG    3255:<<<=:3>>@8@>>?BC:4A;:4:.:@AAACC>>?AABC?=;?A?ABB?DBCCCB@D@E@CB?>?9>AB@CAA?@A?BCCB?B?0/??B<@@=;<;    BD:Z:JJLLQQOMMNONLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKNNNJBLLLNNLNLBMLOJKAK   MD:Z:0C99   RG:Z:0.1    BI:Z:NNPPSRRPPQRPQQQPQNOOMNNPPPPOOPOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQQOIQQQRROROIONNNNHO   NM:i:1  AS:i:99 XS:i:20
HWI-ST115_0148:1:4:6609:9121#0  1024    chr3    58125468    60  100M    *   0   0   CATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG    48629<<A>@?DB??E@@@CBA7@B??C>A@B?@BC@CAC?AB@A?@A@ACB@EBCCCC@EAECCC?A??>@BCC@@?C@@ECCC?C@@8ACCAC>==><    BD:Z:JJMLQQOMMNONLMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKNNNJBLLLNNLNLBMLOJKAK   MD:Z:100    RG:Z:0.1    BI:Z:NNQPSRRPPQRPQQQPQNOOMNNPPPPOOPOPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQQOIQQQRROROIONNNNHO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:28:16780:10277#0   1024    chr3    58125468    60  100M    *   0   0   CAGAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG    48449<<A=@?DB??D@A@CBA7@B??C>@@B?@BB@CAC?@B@@?@A@ACB?DBBBBB@D@EABB?@>??AACB@@>B??BBAB?B@?7@BBAB=;<=:    BD:Z:IINKPQOMMNOMMNMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLKMJA@AJKHIIAKLMMJJKLJAKILLNNMIAKLLOOLMLCLLNIJAK   MD:Z:2T97   RG:Z:0  BI:Z:MMPOQSRPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNHONQQQQPOHQPQQQNQOHONNMMGO   NM:i:1  AS:i:97 XS:i:20
HWI-ST142_0240:1:8:13705:180053#0   1024    chr3    58125468    60  100M    *   0   0   CATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTG    47629<<A=@?DB??D@@@CBA7@B??C>@@B?@BB@BAC?@B@@??B@AB@@DABBBB?D@DBBB?@>??BACB@@>B??DBBB>B@?7@BB@B=;=<:    BD:Z:IIMLQQOMMNOMMNMLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLKMJA@AJKHIIAKLMMJJKLJAKILLNNMIAKLLOOLMLCLLNIJAK   MD:Z:100    RG:Z:0  BI:Z:MMPORSRPOPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNHONQQQQPOHQPQQQNQOHONNMMGO   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:45:17861:199730#0  0   chr3    58125469    60  100M    *   0   0   ATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGT    46249:@>??DA??DA@@CBA8@B??C?A@B?@CC@CAC?@B@@>ABA@CB@EBCCCCBEAECCB?@>>?BBCC@A?C@@ECCB?B@@8ACDAD???=:<    BD:Z:JJLNQPNMOONMMMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMNNJBLLLNNLMLBLMOKKAKJ   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSRQPQRPQQQPQOOOMNNPOQPNPPNPQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPOIQQQRROROIPOOLNHON   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:26:8574:182738#0   1024    chr3    58125469    60  100M    *   0   0   ATAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGT    2438;:;<<<@A@:C===???6=A@;?<>=A?BCA=A@CABA@A=>?>@B>;:>@B@B>D>B@A@?@???:;@@@?>BA?>AC?>BA?7>?;??><9:57    BD:Z:IILMROOMNPMMNNLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJMJAA@JKHIIAKLMMJJKLJAKILLNNMIAKLLNOLMKCLLOJJAKI   MD:Z:100    RG:Z:0  BI:Z:MMOPSRROPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGPMQQQQPOHQPQQQNPOIPNPKMGOM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:46:21305:107554#0  1024    chr3    58125469    60  100M    *   0   0   ATANTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGT    222%9:;<<<DB?>D@@@CB@8AB??C?@@B?ABB>CAC?AA@@?@A@ABB@DBBBBB@D?CBBB??>>?BABB@A>B@>7ABB?B=@7@B@@B@>==27    BD:Z:IILIMLOMNPMMNNLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJMJAA@JKHIIAKLMMJJKLJAKILLNNMIAKLLNOLMKCLLOJJAKI   MD:Z:3G96   RG:Z:0  BI:Z:MMOMOOROPQOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGPMQQQQPOHQPQQQNPOIPNPKMGOM   NM:i:1  AS:i:98 XS:i:20
HWI-ST115_0148:1:42:17429:61202#0   0   chr3    58125470    60  100M    *   0   0   TAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT    42447><?>DB??D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@A?ACB@EBCCCCBDAECCC?A??>ABCCAA?C@@ECCB?C@@8ACCBD?@A>:<>    BD:Z:JJNNPONOPNMNMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMNJBLLLNNLMKBLLPKLAKJK   MD:Z:100    RG:Z:0.1    BI:Z:NNQQSQQQRPQQQPQOPOMNNPOPQNOQNOQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNIQQQRROROIPPPMLHONN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:64:7391:109414#0   1024    chr3    58125470    60  100M    *   0   0   TAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT    42447><?>DB??D@@@CBA7AB??C>@AB?@BC@CAC?AB@A>@A@ACB@EBCCCCBD@DCCC?@>>?@BCC@A?CA?ECCC?CA@7ACC@C?@A>:;>    BD:Z:JJNNPONOPNMNMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMNJBLLLNNLMKBLLPKLAKJK   MD:Z:100    RG:Z:0.1    BI:Z:NNQQSQQQRPQQQPQOPOMNNPOPQNOQNOQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNIQQQRROROIPPPMLHONN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:66:14210:184691#0  1024    chr3    58125470    60  100M    *   0   0   TAGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT    32469><@>BA?@EA??BC@8:BA>C=?ABAACC@@@C@?A??>??>AA=@BAAACC@A@C@@B9@??=@BBC@B0=?;07/;4>A?7@BC=C@@??:<%    BD:Z:JJNNPONOPNMNMMNJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMNJBLLLNNLMKBLLPKLAKJK   MD:Z:100    RG:Z:0.1    BI:Z:NNQQSQQQRPQQQPQOPOMNNPOPQNOQNOQQOHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNIQQQRROROIPPPMLHONN   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:62:5505:107378#0   16  chr3    58125471    60  100M    *   0   0   AGTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTG    >;9=<B??BD??DA@@BA7ABD?@>CBCEBCBAAA@CBBC@>CACABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@C?@>=4684    BD:Z:LLLLMOPPONONKMNJJMKMMMOMLJMMKJAJNLKNKJILLLIJJJJKLMJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJKAJKOJLDLMKIII   MD:Z:100    RG:Z:0  BI:Z:POOPNNPQRQQQOQRPOQOQQPRQPOPPPOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNIPNNNMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:64:11481:16674#0   0   chr3    58125472    60  100M    *   0   0   GTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGT    44267<=CA??D@@@CBA7@B??C>@@B?@BB@CAC?BB@?>?A??A@>BBBBBBAD@DBBB??>>?<BCB@@?B@?CBBB?B??7@CBAC?@BA=;=:9    BD:Z:IILLPPRNNONMNKLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@AAJKGIIAKLMMJJKLJAKILLNNMIAKLLNNKLKBKKOKLCLIJKI   MD:Z:100    RG:Z:0  BI:Z:MMPPQRSOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPQPQPOHQPQQQNPNHPPRNMGMMMOM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:68:15440:147801#0  1024    chr3    58125472    60  100M    *   0   0   GTATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGT    44267<=CA??D@@@CBA7@@??C>@@B?@BB@CAC?@B@?>@B@ACB@DBBBBBAD@BBBB?@>>?BACB@A?B@?DBBB>B??7@@BAB?@BA=;;:9    BD:Z:IILLPPRNNONMNKLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@AAJKGIIAKLMMJJKLJAKILLNNMIAKLLNNKLKBKKOKLCLIJKI   MD:Z:100    RG:Z:0  BI:Z:MMPPQRSOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPQPQPOHQPQQQNPNHPPRNMGMMMOM   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:2:7421:116295#0    0   chr3    58125473    60  100M    *   0   0   TATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTT    4262;;BA>?D>@@DBA8@BA?D?AABAAC>@DAC?AA??=<>;A?>?B@CBCCBDAECCC?@??>A@CC@@>@@?DBB@?B@@6ACC@C?ACCA@>:<=    BD:Z:JJLLQRPNONNOJLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIALLLNNLMKAKKOKLCLKKKJK   MD:Z:100    RG:Z:0.1    BI:Z:NNPPSSQQQQPQOPPNONPOPPNOQNOQPNHOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPQRROROIPPQOOHNLNONN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:63:4580:108191#0   0   chr3    58125474    60  100M    *   0   0   ATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT    46258@@>>D@@@CCA7@B??C>AAA?@CB@DAC?@B@@>AAAACB@EBACBCBEAECCC?@>?>@CCC@A?BA?DCCC?C@@7ACC@C@ACC?@A;;>?    BD:Z:JJLNRQOOONOKLKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKLLNNLMKAKKNKLBMKLKJKA   MD:Z:100    RG:Z:0.1    BI:Z:NNPQTQRQQPQOPPNOOPOPPNOPOOPQNGOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPRROROIPPQOOIOMLONNH   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:6:18428:11442#0    1024    chr3    58125474    60  100M    *   0   0   ATAGCATGCAGACTTCGACGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT    46248@@>>D@@@CBA7@@7?C?@@B?@BB@CAC?@B?@>?A?BCB@DBCCBCBEAECCC?A???ABCC@@?C@@FCCC?C@A8@CCBD@ACC?@A;;=?    BD:Z:JJLNRQOOONOKLKIJKMLKMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKLLNNLMKAKKNKLBMKLKJKA   MD:Z:18T81  RG:Z:0.1    BI:Z:NNPQTQRQQPQOPPNOOPONONOPOOPQNGOPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPRROROIPPQOOIOMLONNH   NM:i:1  AS:i:95 XS:i:20
HWI-ST142_0240:1:1:7175:9732#0  1024    chr3    58125474    60  100M    *   0   0   ATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT    46248@@>>D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@A@=C?@B?BBBBAD@DBBB>@?>>ACBB@@?B@?EBBB?B??7@BBBC?@BB??@:;==    BD:Z:IILMSPPOONNKMLHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@JKHIHAKLMMJJKLJAKILLNNMIAKLLNNKLJAKKNJLCMKKKIJA   MD:Z:100    RG:Z:0  BI:Z:MMOPSQRPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPQOOHQPQQQNPNHPOQOOHOMKOMMG   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:4:12180:29132#0    16  chr3    58125474    60  100M    *   0   0   ATAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTT    89>:=AD??C@??B@8ABD?@?CACD@BB@@@@D@BCA?CAC@BB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>@D?7@BB@@D@BAC>>=4664    BD:Z:LLMNOOOPOKNOKJMKMMMOMLJMMKJAJNLKNKJILLLJJJIJLMNJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IKNIKBJJNLMKAII   MD:Z:100    RG:Z:0  BI:Z:PPNPNPOQRPQRPOQOQQPRQQOQPQOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLPPNNGMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:63:15535:184865#0  0   chr3    58125475    60  100M    *   0   0   TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTT    3031?>><@>99@A<64A?=C>?8BA>CC?C@?@AA?A:>9=?==:BAC==><B45?>?7==<<5/96>7=B@=B@,A?B@?6ACB@B@AC=@A?>>%%%    BD:Z:JJNOQPPOOOKMKIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKLNNLMKAKKNJLBLLLLJKAA   MD:Z:100    RG:Z:0.1    BI:Z:NNQRRRRQPQOPPNOOQOPPNOPNPPPOGNPNOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQROROIPPQOOIPNMMNNHH   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:43:2406:80244#0    1024    chr3    58125475    60  92M8S   *   0   0   TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATACGTTTCTGTTTAGATCGAA    4243=>=>C@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@@@ABBADBBBBBAB@DBBB?@>>>ABCB?A?B@@CCCB?@&=5??@>C@<BB>@@?:+-9    BD:Z:IIMOQPQONOKMLIIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IKHII@KLMMJJKLJAKILLNNMIAKLLLLKLJAJKNJKCLOMMMKMJ   MD:Z:80G11  RG:Z:0  BI:Z:MMPQQRRPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPPNHQPQPPOPNHPOQNOIQPONPNPN   NM:i:1  AS:i:87 XS:i:20
HWI-ST142_0240:1:62:18232:60158#0   1024    chr3    58125475    60  100M    *   0   0   TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTT    3235=>:;@=>=@@?78A>>A>>A@@@B@@B@B@@B??=@:*=<=>B=@<BB>B@CBCB<?>?>A@BB8@>@A=BCBA?A=>7@BB@B@@BB@@8=;;49    BD:Z:IIMOQPQONOKMLIIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IKHII@KLMMJJKLJAKILLNNMIAKLLNNKLJAJKNJKCMKLLIJAA   MD:Z:100    RG:Z:0  BI:Z:MMPQQRRPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPPNHQPQQQNPNHPOQNOIPMMMMMGG   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:8:18333:60211#0    1024    chr3    58125475    60  100M    *   0   0   TAGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTT    4233=<=>C?@@CB?8@A??A?@AB?@BB@B@C?AB@??@A@@B@@DBBBBB@B@@AA@?@>>>AAB@@@?A@<CBBB?B=?8@@B@B@=BB?@A=;<;;    BD:Z:IIMOQPQONOKMLIIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IKHII@KLMMJJKLJAKILLNNMIAKLLNNKLJAJKNJKCMKLLIJAA   MD:Z:100    RG:Z:0  BI:Z:MMPQQRRPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPPNHQPQQQNPNHPOQNOIPMMMMMGG   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:5:18054:24089#0    0   chr3    58125476    60  100M    *   0   0   TGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTG    332:=;<B;@?BB@3@A@?A?@ABAACC>C@CAAC@A?@@@ABB@D@CCCC@E@CCBB>?????BCCA@?B@.5BCA?B@?7@CC@CAACC@@C@??=>;    BD:Z:JJNNPQPOPKMLIJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKNNLMKAKKNJKBLKMLKKAAK   MD:Z:0A99   RG:Z:0.1    BI:Z:NNQPSRRPQOPPNOOQPPPNOPNOQPNHNONOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQOROIPPQOOIPONNLNHHO   NM:i:1  AS:i:99 XS:i:20
HWI-ST142_0240:1:1:6358:13961#0 16  chr3    58125476    60  100M    *   0   0   AGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTG    <9:=B>?CA@@BA7ABD?@>CABDBBCA@A@DACC@>DABABBA?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD?AB<;6684    BD:Z:MNNMNPOLOOKKNKMMMOMLJMMKJAJNLKNKJILLLJJJJJKMNKA@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMIKAIJLJLNEAIII   MD:Z:100    RG:Z:0  BI:Z:NPPPMPPORSPOQOQQPRQQOQQQOHMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNNPIGNMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:22:14034:114749#0  0   chr3    58125477    60  100M    *   0   0   GCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGA    4386;=B?@@BBA7@B??C>@AB?@BB@CAC?ACA@??A??AA?BACCACBDADCCC??>?>@?CCAA?BA=CCCC?BA@7ABCBC@ABC@?CB?B?=:<    BD:Z:JJNMQQPPLMLJJKMLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMNLMKAKKNJKALKLMKLAAKN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSRQQOPPNOOQPQPNOPNOPQNGOOMOPQOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNROIPPQOOIPOOOMLHHOQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:27:14656:55962#0   1024    chr3    58125477    60  100M    *   0   0   GCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGA    3167;;B??>AB<6@@?<A>>>>=@AA>@@@?9A==;>?>9<99<B@BBB@B?BA<=>?;0>@?5:83<1>7<87@>9?>2<@?@CA?A<@A@@@-<559    BD:Z:IIMMRQPOLNLIJKLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGIIAKKMMJJKLJAKILLNNMIAKLLNNKLJAJJMJKBLKLMKKAAKM   MD:Z:100    RG:Z:0  BI:Z:MMOPRRQPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONHPPQQQNPNHPOQNNHQONOMKGGOP   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:43:9839:192700#0   1024    chr3    58125477    60  8S92M   *   0   0   GCTGTGTGGCATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTCT    43739;>>=?DB??D@@@CBA7@B??C>@@B?@BB@CAC?@B@@>@A@@BB@DBBBBBAD@DCBB?A>>?BBCC?A>B??DBBB>B?@7@B@AB>%%%%%    BD:Z:IIMMMLLJNNMMNNLMJLKHIJLKMMLKLJKMLI@JKIILLIIMKLJKJIJLJMJAA@JKHIIAKLMMJJKLJAKILLNNMIAKLLNOLMKCLLOJJAJJ   MD:Z:90G1   RG:Z:0  BI:Z:MMNPOOOMOOOPPPOPOPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGPMQQQQPOHQPQQQNPOIPNPKMGON   NM:i:1  AS:i:90 XS:i:20
HWI-ST142_0240:1:25:16127:188276#0  0   chr3    58125478    60  100M    *   0   0   TATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG    42638@>??CBA7@B??C>@@B?@BB@CAC?AB@@?@B@ABA@DBBBBBAD@DBBB?@>>>A@BB?A?A@@CBCB?B@?8=BBAC?@CB?@B?@A@=9;9    BD:Z:IILNRPQLNMIJKMKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHIAKLLMJJKLJAKILLNNMIAKLLNNKLJAJJMIKBLJLMKLBAKML   MD:Z:0C99   RG:Z:0  BI:Z:MMPPRQROPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGQOQQQNPNHPOQNNHPOOPMMEGOPO   NM:i:1  AS:i:99 XS:i:20
HWI-ST142_0240:1:5:13896:154389#0   1024    chr3    58125478    60  100M    *   0   0   CATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG    48638@>??CBA7@B??C>@@B?@BB@CAB?@B@@?@A@@B@@DABBBB@D@DBBB>@>>>ABBB@@>B??CBCB?B??8@BBAC?@BB?@B?@A@=:;;    BD:Z:IIMNRPQLNMIJKMKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHIAKLLMJJKLJAKILLNNMIAKLLNNKLJAJJMIKBLJLMKLBAKML   MD:Z:100    RG:Z:0  BI:Z:MMPPRQROPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGQOQQQNPNHPOQNNHPOOPMMEGOPO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:64:15528:95249#0   1024    chr3    58125478    60  100M    *   0   0   TATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGGTTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG    42638@>??CBA7@B??C>@@B?@BB@CAC?@B@@?@@@@BB@DBBBBBAD@DBBB>@>>>>@BB?@?B@@DBCB?B?>8=BBAC?@CB?@B?@A@=9;9    BD:Z:IILNRPQLNMIJKMKMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHIAKLLMJJJLJAKILLNNMIAKLLNNKLJAJJMIKBLJLMKLBAKML   MD:Z:0C60A38    RG:Z:0  BI:Z:MMPPRQROPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJJOMGOMPPPPONGQOQQQNPNHPOQNNHPOOPMMEGOPO   NM:i:2  AS:i:94 XS:i:19
HWI-ST115_0148:1:3:18398:22371#0    16  chr3    58125478    60  100M    *   0   0   CATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG    ;==;>A>A?C46@BC???C@BD@DCA?B@E@CC@<D@CADBA@BCA@A?@@ACCB?C???@BCCD@<BE=ABCB>AE?5@CC@?E?CCEABC@A@=:463    BD:Z:NMNNOLPOLLNLNMNONLKNMKJ@JMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIIKJJLDEMLKJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPQNNQRQPRPRRQSRQPQQPOHNQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQNGOOOQOONNGNNQMOHNMONMOIIPPNNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:48:12854:139828#0  1040    chr3    58125478    60  100M    *   0   0   CATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAG    8<?:=BA@@B@7BBD?@>CABDABC@ABAEBBCA>CABABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD>7@BB@@D@BBD@BC?@>;8574    BD:Z:NMMNNLOPLKNLNMMOMLJMMKJAJNLKNKJILLLJJJJJLMMKAAAILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJAIIKJJLDDMKJII   MD:Z:100    RG:Z:0  BI:Z:PPOPNNPRQPQOQQPRQQOQQQPHMROOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNIIPPNMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:41:13159:188033#0  0   chr3    58125479    60  100M    *   0   0   ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGC    4633=<>?BBA7@B??C>@@B?@BB@CAC?@B@@>@@@@BB@DBBBBBAD@DBBB>@>>>>BBB?@?B?@CBAB?C??7@@BAC?@BC?@B?@BA@:;;8    BD:Z:IINNQQNNMJJKMLMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHHAKLMLJJKLJAKILLNNMIAKLLNNKLJAJJMIJBLJKMKLCBKMLO   MD:Z:100    RG:Z:0  BI:Z:MMPPQRQPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPPPQQNPNHPOQNNHPNOQNMGEOPOQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:6:10123:75568#0    1024    chr3    58125479    60  100M    *   0   0   ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGC    4533=<>?BBA7@B??C>@@B?@BA@B@C?@B@@>?A?ABA@DABBBBAD@DBBB?@>>>@BCB?@?@?@CCB@?C??7?BBAC?@BC?@B@@BA@:;:8    BD:Z:IINNQQNNMJJKMLMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHHAKLMLJJKLJAKILLNNMIAKLLNNKLJAJJMIJBLJKMKLCBKMLO   MD:Z:100    RG:Z:0  BI:Z:MMPPQRQPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPPPQQNPNHPOQNNHPNOQNMGEOPOQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:7:13079:161590#0   1024    chr3    58125479    60  100M    *   0   0   ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGTGTTTCTGTTTGTTGTTTTGAGC    4433=<>?BBA7@B??C>?@B?@BB@CAC?@B@@?@A@ABB@DBBBBB@D@CBBB>@>>>@BCB?@?B?@DCBC?A@1@@9B@CA@BC?@B?@BA@:::8    BD:Z:IINNQQNNMJJKMLMMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHHAKLMLJJKLJAKILLNNMIAKLLNIIIJAJJMIJBLJKMKLCBKMLO   MD:Z:77C22  RG:Z:0  BI:Z:MMPPQRQPPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPPPQNNNNHPOQNNHPNOQNMGEOPOQ   NM:i:1  AS:i:95 XS:i:20
HWI-ST142_0240:1:41:10866:49403#0   16  chr3    58125479    60  100M    *   0   0   ATGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGA    =?:;A@@@BA7@CD?@>CABDABB@@A@EBCB@?BACBBBA?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD?A@>=5754    BD:Z:MMNMKOPLLNLNNMOMLJMMKJAJNLKNKJILLLJJJJJLMNJAAAJLHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@IIKIJLBDLOJJII   MD:Z:99C0   RG:Z:0  BI:Z:POPPLPQPPROQQPRQQOQQQPHNQPOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGIPRNNMM   NM:i:1  AS:i:99 XS:i:20
HWI-ST115_0148:1:5:2026:25837#0 0   chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA    33389=>BBA7AB?@C>@@B?ABBACAD?@BA@>@A?ACB@DBCCBC@E@DBAC?@=>?@BCAAA?CA?DCCB?BAA9ACC@D@ACCAAC?ACCA?>:7=    BD:Z:JJNNRNOMKKLNLMMMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLKAKKNJKAKJKLKLCBLNLON   MD:Z:100    RG:Z:0.1    BI:Z:NNQPSPQPNOOQPQQOPPNOPPNGOOMOOPOPQQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHPPQOOIPOOPOOHGMQPRP   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:68:7477:156944#0   1024    chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA    43299<>BA?7@B?>@><=A?AB?@B>B?>A>A?@A>@?>>B?@@BB>D=BB@@=?>>=ABB@?A>BA?AB6@>@@>7@B=@A@@BB@@A@@BBA>=:.=    BD:Z:IINMRNPMJKKMLNMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@KLMMIJKLJAKILLNNMIAKLLNNKLJAJJMIJALJKLKLCCLMLOM   MD:Z:100    RG:Z:0  BI:Z:MMPORQRPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOQPQNPNHPOQNNHPNNQONGGMPOQO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:7:16006:51811#0    1024    chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGCTTCTGTTTGTTGTTTTGAGCA    43389<>BAA7@B??C>@@B?@BB@CAC?AB@@>@A@@BB@DBBBBBAD@DBBB>@>>>ABBC?@>C??CBCB?B@?8?BBAC?@BB?@A?@BBA=<:8=    BD:Z:IINMRNPMJKKMLNMLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@KLMMIJKLJAKILLNNMIAKLLNNKMMIJJMIJALJKLKLCCLMLOM   MD:Z:78T21  RG:Z:0  BI:Z:MMPORQRPNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOQPQNPOOPOQNNHPNNQONGGMPOQO   NM:i:1  AS:i:95 XS:i:20
HWI-ST115_0148:1:5:5308:32801#0 16  chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA    >::>@@@BA8BCE@A?CBBE?DCABA@E@CDA?D?D@CBA@BCCCCA@A@BCB>C@?@ABBCD@>BD??BBB>BD?7@BB@@D@BBD@BD@AA@@:7334    BD:Z:NNNKOOMLOMNMNONLKNMLK@JMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJJIKCCLONNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQPOOPPPRPRRQSRQPRRPOHNQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHNOPPOPNNGNNPLOHNMONMOHHOQPOQNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:22:2585:58573#0    1040    chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA    :::>??@BA7ABE?@>CABD@BB@@AADBCD@>DACACB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@AA@@:7334    BD:Z:MNMJNPLLOLNNNOMLJMMKJAJNLKNKJILLLJJJJJLMNK@AAJMHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HIKIILBBLNNMNII   MD:Z:100    RG:Z:0  BI:Z:OPPNNQOORPQQPRQQOQQQPHNROPPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGPRPNPMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:44:11750:170821#0  1040    chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA    =::=>?@B@7@BE?@>CABDABBAAA?E@CD@?DABACAA?BBBBB@?@?BBB>C@>>ABBBD@>BD??BBB>BD?7?BB@@D@ABD@BC?AA@@:7324    BD:Z:MNMJNPLLOLNNNOMLJMMKJAJNLKNKJILLLJJJJJLMNK@AAJMHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HIKIILBBLNNMNII   MD:Z:100    RG:Z:0  BI:Z:OPPNNQOORPQQPRQQOQQQPHNROPPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGPRPNPMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:46:11710:142327#0  1040    chr3    58125480    60  100M    *   0   0   TGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCA    ?98>??@B@8ABE?@>CABD@BB@@@AEBBD@>DACACB@?BBBBB@?@?BBB>C@@@ABBBD@?BD??BBB>BD?7@BB@@D@BBD@BC@AA@@:7334    BD:Z:MNMJNPLLOLNNNOMLJMMKJAJNLKNKJILLLJJJJJLMNK@AAJMHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HIKIILBBLNNMNII   MD:Z:100    RG:Z:0  BI:Z:OPPNNQOORPQQPRQQOQQQPHNROPPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGPRPNPMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:64:19711:152416#0  16  chr3    58125480    60  10S90M  *   0   0   TTCCGATCTTTGCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTT    7961<1?>BBB>@B@@?BA6@BD>@?B@BD>@B=?@@D@BC@?C@C@@@@@BBBBABA8?BB>?A>>>@>BBB=>>D??BBB??D=6>AA@>B>>;8354    BD:Z:JKLKNNMLCJNONJMNJJMKMMMOMLJMMKJAJNLKNKJILLKJJJJIKLMJ@@@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLKKAJLOJNDMIKII   MD:Z:90 RG:Z:0  BI:Z:NPMNNPOPIPPQQOQRPOQOQQPRPQNPPPOGMQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLPIPLNMM   NM:i:0  AS:i:90 XS:i:20
HWI-ST142_0240:1:44:16206:105260#0  0   chr3    58125481    60  100M    *   0   0   GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG    3275;<?@>5><<2=>>@@?@BA?B@B>AB8;=<???A@>B=@@@B@D@DBB@???>>@?AB@@?@?=DABB?B@?7@BB=B@@?BA?>>9?2:=?;885    BD:Z:IIMNOPOJKLMLNNLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JLMMJIKLJAKILLNNMIAKLLNNKLJAJJMIJAKJKLJLCCMNLOMN   MD:Z:100    RG:Z:0  BI:Z:MMOPQRRNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPQPNPNHPOQNNHPNNPOOHGONOQOP   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:65:13196:26849#0   1024    chr3    58125481    60  100M    *   0   0   GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG    43849<AA@7@B??C>@@B?@BA@CAC?@B@@>@@?ABA@DBBBBBAD@DBBB>@>>>@BBB@@>B@?DBCC?C??7@BBAC?@BB?AB?@BBB>?;8>6    BD:Z:IIMNOPOJKLMLNNLKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JLMMJIKLJAKILLNNMIAKLLNNKLJAJJMIJAKJKLJLCCMNLOMN   MD:Z:100    RG:Z:0  BI:Z:MMOPQRRNONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPQPNPNHPOQNNHPNNPOOHGONOQOP   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:27:14769:95346#0   16  chr3    58125481    60  100M    *   0   0   GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG    :;=<?>3A7@BD???C@AD@CDABA@EAADA?B@D@?B@ABCBCBA?@@BCB?D??=ABCCE@?BD?@:CB?BD>5@ACAAEABCE@CEABBBC>>4463    BD:Z:NNKNOLMOMOMNONLKNMLKAJMKJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIIKBCKNNQNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPOQOOORPRRQSRQPRRQOHNQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOOOQOOONGNNPLNHNMONMOHHNQOQQPNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:67:19258:137763#0  1040    chr3    58125481    60  100M    *   0   0   GCAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAG    89;;>?BA7@BD???C@BD>BB@@@?EBBBA>BBCABB@?BBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@AD@BAAB=<3374    BD:Z:NMJMOLLOMNNNPMLJMMKJAJNLKNKJILLLJJJJJLMNKA@AJMIHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHKIIKBBJNMQNMII   MD:Z:100    RG:Z:0  BI:Z:PPNPOONQPRQPRQQOQQQPHNRPOQPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNRPPPPMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:8:18119:188547#0   0   chr3    58125482    60  100M    *   0   0   TAGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATCTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGG    42449?@?6@A??C>@@B?@BB@AAC?@B@@?@A@ABB@DBBBBBAD@DBBB>@>>>:A@A@@?A@?CBBB?A@>8@BB@C?@BB?@B?>BBB@?:9<85    BD:Z:IIMKQOLKLNLNNMKLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKMMJJJLMJMILLNNMIAKLLNNKLJAJJMIJAKIKLJKCCMOMOMNM   MD:Z:0C58T40    RG:Z:0  BI:Z:MMPORRPONPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPPNPMPPPPONGPOPPQMPNHPOQNNHPNNPNOIHOPMQOPO   NM:i:2  AS:i:94 XS:i:19
HWI-ST115_0148:1:24:6939:189286#0   0   chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444<>?6?B??C>@@B?@BB@CAC?@B@@?AAAACB@EBBBBBAD@DCBC>A???ACCC@A?C@@DCCC?C@?8ACCBD@ACC@@C@ACCC@A@?A;9<    BD:Z:JJKMOMMMOMNNMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JKNJKAKJKKJKBBLPMPNONM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPROPOQPQQOPQOPPPNGNOMOOPOOPQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPOOIPOOPOOIIPQOPPQOP   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:28:5159:97290#0    1024    chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444>>?6@B??D?@AA?@BCAAAC?AAA@>?@>ACB@DBCCBCAE@EBCC?A??>AACB@A?BA>DCCB?BA@7@DCBB@ACC@AB@@CCC@=@>A:8<    BD:Z:JJKMOMMMOMNNMKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JKNJKAKJKKJKBBLPMPNONM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPROPOQPQQOPQOPPPNGNOMOOPOOPQQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPOOIPOOPOOIIPQOPPQOP   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:23:11033:120892#0  1024    chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444<>?6?B??C>@@B?@BB@CAC?@B@@?@A@ABB@DBBBBBAD@DBBB>@>=>A@BB?A?B??DBBC?B@@7ABBAC?@BB?AB?@BBB?A>=?:9;    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML   MD:Z:100    RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:23:5024:179493#0   1024    chr3    58125483    60  98M2S   *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGAG    3246<>=5?@><B?<@B>@BA@C?B?@B@?>?A>?B5=B@>;<>;;>:<664+;6?BBBB?9>??>B6A@?A@?7@B@@B;@BBA@B@@9BB:<>=?734    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNKL   MD:Z:98 RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:98 XS:i:20
HWI-ST142_0240:1:2:14618:76577#0    1024    chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444<>?6?B??C>@@B?@BB@CAC?@B@@?@A@@BA@DBBBBBAD@DBBB>@>>>@BBB?@?B??DBBC?C@@7ABBAC?@BB?@B@@BBB@@?=?;97    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML   MD:Z:100    RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:46:19190:95037#0   1024    chr3    58125483    60  98M2S   *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGAG    4444<>?6?B??C>@@B?@BB@CAC?@B@@??A??A@>B@BBBBAD@DBBB?@>>>ABBB?@?B??CBAB?C@@7ABBAC?@BB?@B@@BBB?A?=?;;6    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNKL   MD:Z:98 RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:98 XS:i:20
HWI-ST142_0240:1:47:11226:2414#0    1024    chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444<>?6?B??C>@@B?@BB@CAC?@B@@?@@@@BA@CABBBBAD@DBBB>@>>>ABBB?@?B??CBBB?C@@7ABBAC?@BB?@B@@BBB?B?=?;99    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML   MD:Z:100    RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:6:10870:168832#0   1024    chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444<>?6?A??C>@@B?@BB@CAC?@B@@??A?<BA@DBBBBB@D@DBBB>@>>>@BBB?@?B??BBBB?C@?7ABBAC?@BB?@B@@BBB?@?=?;89    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML   MD:Z:100    RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:8:3362:36600#0 1024    chr3    58125483    60  100M    *   0   0   AGACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGT    4444<>?6?A??C??@A?@BA@C@C?AB@>??B?BBA@CABBBBAD@DBBB>@>>>@@BBA@>:@>CAA@?B@?7ABBAB=A@B?AB@?BBB?A?=?777    BD:Z:IIKMPLMLNMNNMLLJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLMJJKKJAKILLNNMIAKLLNNKLJAJJMIJAKIJLJKBCMONPMNML   MD:Z:100    RG:Z:0  BI:Z:MMOPRPQNPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPNONHPOQNNHPNNPNNIIPPOOOPOO   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:45:20043:38425#0   0   chr3    58125484    60  100M    *   0   0   GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC    4447;=5?A??C>@@B?@BB@@AC?@B@A>?A@ACB@EBCBBBAD@CBBB?@???ABCC@@?CA@DCCB?BA>7ACCBD@@DC@AC@ACCC@@A?D;9<;    BD:Z:JJMLMNNONNNNKMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJNJKAKJKKJKABLONPOONMN   MD:Z:100    RG:Z:0.1    BI:Z:NNPPPPPQPQQOPQOPQPNGNOMNPPNPPPQOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNOIPOOPOOIIPRPQNQOPQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:41:16470:171070#0  1024    chr3    58125484    60  100M    *   0   0   GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC    4437;<6??>>C>@@A?@AB@A@A@@B@???A@BBA@DABBBB@C@DBBB>?>>>;ABB@@?A@>CB;A?BA?7@BB@C?=B@?@@?@>BB?A?>@;7:9    BD:Z:IIMLMMNNMONMLMJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLJJKLIAKILLNNMIAKLLNNKLJAJJMIJAKIJKJKBBMONQNNMLM   MD:Z:100    RG:Z:0  BI:Z:MMPPPQPPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMPMHPOQNNHPNNPNNHIQQOQMPOOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:47:19503:63851#0   1024    chr3    58125484    60  100M    *   0   0   GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC    4447;=5?A??C>@@B?@BB@CAC?@B@?>=@>9@@?A?@BBBAD?DBB@>@>>>ABBB?@?B??CCB@?A@?7@BB@B@7@???@??>>B?B@>B;8;9    BD:Z:IIMLMMNNMONMLMJKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLJJKLIAKILLNNMIAKLLNNKLJAJJMIJAKIJKJKBBMONQNNMLM   MD:Z:100    RG:Z:0  BI:Z:MMPPPQPPPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMPMHPOQNNHPNNPNNHIQQOQMPOOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:22:18100:32416#0   16  chr3    58125484    60  100M    *   0   0   GACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTC    =;;>?7@BE@@?DBCFA>DAAABF@CDA?D@DBCCA@CCCCCA@@@CCB?C@@@ABBBD@?BD??BBB>BD@7@BB@@D@BBD@BD@BBBD@B=;<4444    BD:Z:NNKKOMPNOPNLKNMLKAKNLJNKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJABJLLOPQOMMJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPOONQRQSRQPRRQPIOQPOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQOOONGONPLNGMLONMOHHNPNORQROPNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:41:13824:159325#0  0   chr3    58125485    60  100M    *   0   0   ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT    4476:3>A@?C?@AB?@CB@C@C?ABA@>@A?ACB@DBCCBBAE@DBCB?A>?>ABCCAA?BA@DCCB=BA?7ACDBC@@DC@AC@ACCC@@A?D?;;;@    BD:Z:JJLJNOPNONNLMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMJKAKJKKJKAALOMQOPNMNK   MD:Z:100    RG:Z:0.1    BI:Z:NNPNQPRPQQOPQOPQQNGNOMNOQNOQPPOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNIPOOPOOIIPRQROOOPQO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:67:14854:93529#0   1024    chr3    58125485    60  100M    *   0   0   ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT    4476:3>A>@C>@@B?ABB@CAC?@C@@?@A?ACBAEBCCBC@D@DBCB>A>?>ACCC@A?B?@EBCC?C@?8ACDBE@@CC@AC@ACBC@AA?E>:;;=    BD:Z:JJLJNOPNONNLMJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMJKAKJKKJKAALOMQOPNMNK   MD:Z:100    RG:Z:0.1    BI:Z:NNPNQPRPQQOPQOPQQNGNOMNOQNOQPPOPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNIPOOPOOIIPRQROOOPQO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:7:1891:111214#0    1024    chr3    58125485    60  100M    *   0   0   ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT    4476:3>A>?C>@@B?@BB@CAC?@B@@>@A@@BB@DBBBBB@D@DBBB>@>>>BBBB?@?B@?DBBA?C??8@BB@C???B?@B?@CBB?@??C<9;<>    BD:Z:IILINNPMOOMLMKKMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIJKLJ@KILLNNMIAKLLNNKLJAJJMIJAKIJKIKBBLONQOOMLMJ   MD:Z:100    RG:Z:0  BI:Z:MMPNQPRPPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMONGPOQNNHPNNPNNHHQRPQONOOPN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:1985:183505#0   16  chr3    58125485    60  100M    *   0   0   ACTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCT    9:<<5?BD>@?C@BD@BB@>@@D@BB@?B@B@BB@@@BBAB@?@?BBB?C@@>@BABD@?BD??BBA=AD?8@BB@@D@ABD>BD@BBBC?B>=?94334    BD:Z:NJJMLOOOQNMKNMKJAJNLKNKJILLLJJJJJLMNKAAAJMHIKAJKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJAAIKKNOPOPMKII   MD:Z:100    RG:Z:0  BI:Z:QONPLPPPSRQOQQQPHNRPPQQPNQOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPRRQPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:67:14903:30979#0   0   chr3    58125486    60  100M    *   0   0   CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC    47650<@>>C>@@B?@BB@CAC?@B@@>@@AACB@DBBBCBAE@DCAB>>?>>ABCCAA?BA@ECCB?BA@8ACCBC@@CC@AC@ACCC@@@?E@<=:>:    BD:Z:JJJKOQOOONLNJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIKAKJKKJKAAKOMPPPOMNKK   MD:Z:100    RG:Z:0.1    BI:Z:NNNOQRQQQOPQOPQQOGNOMNOPOOPQPNPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHPOOPOOIIPRQSPPMPQON   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:8:17184:151826#0   1024    chr3    58125486    60  100M    *   0   0   CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTA    37651=?>>A>=@B@@BC@C>CA>C>>>=A?ABB>@A?>BC@E@<CCA=?>==@BCCA@?B?:>A<B=BA=7A@A;:=99C@@@@@BCCA3=>@?;=9<8    BD:Z:JJJKOQOOONLNJLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIKAKJKKJKAAKOMPPPOMNKL   MD:Z:99C0   RG:Z:0.1    BI:Z:NNNOQRQQQOPQOPQQOGNOMNOPOOPQPNPOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHPOOPOOIIPRQSPPMPQOP   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:24:10894:139937#0  1024    chr3    58125486    60  100M    *   0   0   CTTCGATTCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC    47550=@A@C>@@=?@BB@CAC?@@@@=@@@ABB@DBBBBBAD@DBBB?@>>>@BBB?@?B?@BBAB<A@>7@BB@C??BB?@B?@BCB?@>?D?<;7;<    BD:Z:IIIJOPOKKKLMKLMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIKLJAJILLNNMIAKLLNNKLJAJJMIJAKIJKIJBBLNNQOPNLMJJ   MD:Z:7G92   RG:Z:0  BI:Z:MMNOPRRNONPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMHOOQNNHPNNPNNHHPRQROPMOPNN   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:4:3218:151835#0    1024    chr3    58125486    60  100M    *   0   0   CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC    46543<@>>B>@@<?@BB@B@C?@B@>?@@<8A>=CABBBB?D@DBBB?@>>>AABB@@?A??DBB@?BA?8A@@@C@AB@?@B??BBB?>??B>;<9:7    BD:Z:IIIJOPOOONLMKLMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIKLJAJILLNNMIAKLLNNKLJAJJMIJAKIJKIJBBLNNQOPNLMJJ   MD:Z:100    RG:Z:0  BI:Z:MMNOPRRPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMHOOQNNHPNNPNNHHPRQROPMOPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:7:15227:67080#0    1024    chr3    58125486    60  100M    *   0   0   CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC    47650<@>>C>@@B?@BB@CAC?@B@@>@@?@BA@DB@BBBAD@DBBB>@>>>ABBB?@>B?@DBBC?B@?8@CBBB?@BB?@B?@BCB?A@?D?<;<7<    BD:Z:IIIJOPOOONLMKLMLI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIKLJAJILLNNMIAKLLNNKLJAJJMIJAKIJKIJBBLNNQOPNLMJJ   MD:Z:100    RG:Z:0  BI:Z:MMNOPRRPPNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMHOOQNNHPNNPNNHHPRQROPMOPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:62:18957:42396#0   16  chr3    58125486    60  100M    *   0   0   CTTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTC    :;;3=BD@B?DBBE@DC?BA?FBCD@?DBD9CCA@BCCCCA@AACCC?CA@?ABCBFA>BD@?BBB>BE?7@BB@@D@BBD@BD@BBBDAC>>A<94444    BD:Z:KKNLONPPOMKNMLKAKNLKOKJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAILKNOONPPKLJJ   MD:Z:100    RG:Z:0.1    BI:Z:POQOOPPSRQPRRQPIORQOQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPNNHNNQLNGMLNMMOHHNPNOQPQPROPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:27:20341:130547#0  0   chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGTGCAGGTGTCC    063-<<>=7>>?A@@B?>C@B@?C?=<=><ACB?AAA>>>=C>>ABB???59<AAC@A;B@??CCB<1??39<=<CBACCAAB@A%%%%%%%%%%%%%%%    BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKJJOOQONIJNM   MD:Z:89A6C3 RG:Z:0.1    BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPONRQQNNMNQO   NM:i:2  AS:i:91 XS:i:20
HWI-ST115_0148:1:45:19946:11303#0   1024    chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    364,;?=<B?>=A@ABB?C@CAAC@>:>=?4@=8??B@=@AC@ACAA=??>?@ACC@A?B@>BBC@?BA?7A@B@C@ACCA@CAACD<@A=<B@<?;=;8    BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKNMPOQONNKKM   MD:Z:100    RG:Z:0.1    BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPRQSQQNNQONO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:47:9971:161282#0   1024    chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    363,;>=?>=>@A@?BB=@@D?>C>?68::9>?67?A?A?@E@>CAB>=+52>;@CA@?B?<C?+B=@4=8@CB@C?ACC@AC?>CBC@A@5C3>?:<97    BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKNMPOQONNKKM   MD:Z:100    RG:Z:0.1    BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPRQSQQNNQONO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:6:6832:181239#0    1024    chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    365+;>=>C?A@AAABB@CAC?AB@A??A?>BA?CBCCCCADADCBB>@>>>ABCD@A?CA@ECCC?B@?7ACC@E@ACC@AD?ACDC@4@>D=<?<<;7    BD:Z:JJKLQPPOOLNKLMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJAKJKKJKAAKNMPOQONNKKM   MD:Z:100    RG:Z:0.1    BI:Z:NNOOSQRQOPQOPQQOHNOMNOPNPPPQNOOOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHOOOPOOIIPRQSQQNNQONO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:22:11619:171791#0  16  chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAGACTGTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    >=2=AD?A?DABEBD>@?D*EBCCB?DBC@CBA@BCCCCA@A@CCC?D@A@ABBCD@>BD??BBB>BD@7@BB@@D@CBD@CD@BBBDAC?>A><94424    BD:Z:KNLNNOQOMLNMLKKNNOIILJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKKNNOMOPNLLJJ   MD:Z:15A3C80    RG:Z:0.1    BI:Z:OQOQOORRQPRRQOPRRRNMPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPONGONPMNGMLNMLOHHNPNOQPPPQQPPNN   NM:i:2  AS:i:90 XS:i:20
HWI-ST115_0148:1:63:20138:120245#0  1040    chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    <<2;AD???DABEBCD@?BBEBCDA?A@DBCCA@CCCCCA@A@CCC?D@A@ABBBE@?BD??BBB>BD?7@BB@@D@BBE@BD@BBBDAC?>B><94424    BD:Z:KNLNNOQOMLNMLKAKNLKOLJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKKNNOMOPNLLJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQOQOORRQPRRQPIORQPQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPONGONPMNGMLNMLOHHNPNOQPPPQQPPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:6:10123:95999#0    1040    chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    >=1<@D???C>@C>=B=?A@E@CC@?C@A@=4AABBCCCAAA@CBB?C>?=>B??B@<BD9@CCB?BE?5@CCA@A?;>>>AB=@CCC?B>9@<<<45-3    BD:Z:KNLNNOQOMLNMLKAKNLKOLJIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKKNNOMOPNLLJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQOQOORRQPRRQPIORQPQPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPONGONPMNGMLNMLOHHNPNOQPPPQQPPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:1:14398:125110#0   1040    chr3    58125487    60  100M    *   0   0   TTCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCC    ;;2;@C?@>C@AA@BB@@@@DABC@?DBCBCB@@BBBBBA@@?BBB>B@@@@BBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC?>B><94314    BD:Z:JMKMNOQONKNNLJAJNLKNKJILLLJJJJJLMNKAAAJMIIJAJLLKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@IKJMONMOPOLKII   MD:Z:100    RG:Z:0  BI:Z:NPNPNOQQRPQQQPHNRPPQQPOQOQNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPQRQOPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:68:17268:187738#0  0   chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAGCTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    45+4;<=C>@@B?@C<>C@C?AB@@??A??CB@DBBBBCAD@DCCB?A?>>>BCC@A?@A@ECCB?B@@7ACB@?@ACC@ACA@CCB@@??C@8@??;:=    BD:Z:JJLNPQPOMNKMMMJJNMJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@KJKKJKAAKNLPOPPNOKKMM   MD:Z:15A84  RG:Z:0.1    BI:Z:NNOQRRROPQOPQQOPRNMNOPNOQPPOONOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONOPOOIIPRQSQROOOONOQ   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:68:4020:121175#0   1024    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    45+4;;=B=@@B?@BB@CAC?@BA@?@A@ACB@EBBBBBAD@DBBB>@>>?ACCC@@?B@@ECCC?BA@8@CC@C@ACC@@C@@DCC@A?>F??@@?;;>    BD:Z:JJLNPQPOMNKMMMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@KJKKJKAAKNLPOPPNOKKMM   MD:Z:100    RG:Z:0.1    BI:Z:NNOQRRROPQOPQQOHOOMNOPNOQPPOONOPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONOPOOIIPRQSQROOOONOQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:23:11356:171933#0  1024    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    45+4;;=B=@@B?@BB@CAC?@B@@?@@>@BB@DBBBBBAD@DBBB>@>>>ABBB?@>B??DBBB?C??8ABBBB@@BB?@B?@BBB@A@?D@>??><:?    BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL   MD:Z:100    RG:Z:0  BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:28:7496:9381#0 1024    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    32+9<;9?<=@>@=@<:@:?==@5>:696><83@6@A>@<A?BCAB5<>>=@9@@A??=><1<@@?B>=6>4@>9??@;?;@?AB:@:.>>B?%=9=439    BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL   MD:Z:100    RG:Z:0  BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:46:17107:126330#0  1024    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    45+4;;=B=@@B?@BB@CAC?@B@@?@@@?BA@DBBBBBAD@DBBB>@>>>ABBB?@>B??DBBB>C??8@BB@B@@BB?@B?@BBB?A@?D@>??><;?    BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL   MD:Z:100    RG:Z:0  BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:48:18179:38080#0   1024    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    45+4;;=B=@@B?@BB@C@C?@B@@?@A@>BA@CABBBBAD@DBBB>@>>>ABBB?@>B??DCBB>C??8ACCBB@@BB?=B?@BBB@@@?D?>?>><8?    BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL   MD:Z:100    RG:Z:0  BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:8:11819:138804#0   1024    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    45+4;;=B=@@B?@BB@CAC?@B@@>?A?<@?>A>BBBBAD@DBBB>@>>>ABBB?@>A??CCBB>C??8@BC@B@@BB?@B?@BBB@A@?D???<>:8:    BD:Z:IIKMPQQNMNKLNMI@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKJAKIKLNNMIAKLLNNKLJAJJMIJAKIJKIJAALNMPOPONNJJLL   MD:Z:100    RG:Z:0  BI:Z:MMNPRRRNPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGOOPNNHPNNPNNHHPQPSQQOONNNOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:6:5048:176068#0    16  chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    ;2;>D@??D@BEADCAA@BFBCDB?DBCACCA@BCCCCA@A?CCB?DA@AABCBE@>BD@?BBB>BD?7@BB@@D@CBD@BD@BBBDAC??B?><94234    BD:Z:NLNMOPPMLOMLKAKNLKOLKIKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJNNNMNONOLMJJ   MD:Z:100    RG:Z:0.1    BI:Z:QOQQNQQQPRRQPIORQPRPONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOGNOPLOGMLNMLNHHNPNOQPPOQPRPONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:26:9631:21633#0    1040    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    82<=B>@>C@BDBBBA@B?AABC@?CBCBCC@?CBBBB@?@?BBB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBADAC??B?><94234    BD:Z:MKMMNQONLNNLKAJNLKNKJILLLJJJJJLMNKAAAJMIIK@JLLLIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HKJMNNMMPNPKMII   MD:Z:100    RG:Z:0  BI:Z:PNPPMQPQPRQQPHNRPPQQPOQPPONNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPORQQPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:2:15091:184337#0   1040    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    71:=B>?>C@BD@BB@@@@A@BC@?C@C@B@A@ABBBAA?@?BBB?C>@>@ABBD@?BD??BBB?BD?7@BBA@D>ABD>BD?BBBC@C>>B<><94233    BD:Z:MKMMNQONLNNLKAJNLKNKJILLLJJJJJLMNKAAAJMIIK@JLLLIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HKJMNNMMPNPKMII   MD:Z:100    RG:Z:0  BI:Z:PNPPMQPQPRQQPHNRPPQQPOQPPONNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPORQQPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:48:9330:156263#0   1040    chr3    58125488    60  100M    *   0   0   TCGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCA    :2<=B>@>CABDBBB@@@@AABC@?CBD@CC@?CBBBC@?@?BBB>C@@@ABBBD@>BD??BBB>BD?8@BBA@D@BBD@BD@BBBCAC??B?><94234    BD:Z:MKMMNQONLNNLKAJNLKNKJILLLJJJJJLMNKAAAJMIIK@JLLLIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HKJMNNMMPNPKMII   MD:Z:100    RG:Z:0  BI:Z:PNPPMQPQPRQQPHNRPPQQPOQPPONNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPORQQPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:27:7074:100817#0   0   chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    4+468;A=?@B?@BB?CAC?@C@@>@A@ACBAEBCBBBAD@DCCB>@>>>ABCC@A?BA@ECCB?CA@7ACCBD@ACC@@C@@CCC@A@@D=?@AA=8?=    BD:Z:JJNMQQPMOKMNMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JJKKJKAAKNLOOPOOOLKMMM   MD:Z:100    RG:Z:0.1    BI:Z:NNQPSRPPQOPQQOHOPMNOPNOPQPNPNNPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNPOOIIPRQSQRPPPMNOQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:28:19225:82436#0   1024    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    3+469=B=?@A?@CC?DAD?AC?A?>>=8@??CAACCCAD@CCAB?A>>>?BCCA@?BA?DBBB?B?>7ACC@C@ACC@@B@ACBC@A?>B=<@<@<8=<    BD:Z:JJNMQQPMOKMNMJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JJKKJKAAKNLOOPOOOLKMMM   MD:Z:100    RG:Z:0.1    BI:Z:NNQPSRPPQOPQQOHOPMNOPNOPQPNPNNPQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNPOOIIPRQSQRPPPMNOQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:22:14768:183117#0  1024    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    2*46::?<=@A?@AA?A?A@@B?>??A=AB>>A@BBBB@C@BB?B??>???@B@@@?@@>C=C@=BA?7@A>?B@@B@@A6@@B@B@A??D?>>??:7;8    BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIJKIJAAKNMPNPONOKJLLM   MD:Z:100    RG:Z:0  BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNNPNNHHPQPRQRPOPLNOPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:3:14548:68998#0    1024    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    4+468;A=?@B?@BB@CAC?@B@?>>@?@BA@CABBBBAD@DBBB>@>>>ABBB?@>B@?DBCB?B@?7@BBBB?ABB?@B?@BBB?A@?D@>@@?<;?=    BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIJKIJAAKNMPNPONOKJLLM   MD:Z:100    RG:Z:0  BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNNPNNHHPQPRQRPOPLNOPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:43:4125:134378#0   1024    chr3    58125489    60  97M3S   *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTCGTTTTGAGCAGGTCTCACT    3+56<;?==?A?@AA@C@C?@A?=??@=>AB@DBBBB@@B@BC?B?>=>=>;@8?>?BA?BBB?>BA>8@@B@AA<@@@@&4??=:0:48D@>@??:%%%    BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIMKLJAAKNMPNPONOKJKHL   MD:Z:80T16  RG:Z:0  BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNQOPNHHPQPRQRPOPLNPLP   NM:i:1  AS:i:92 XS:i:20
HWI-ST142_0240:1:68:5688:125012#0   1024    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    3*57;:?==@>?@BB@?AA?@A>@????ABA>CABBBBAD@D@B?=@>>>ABBB@??B@?BAA@>BA>7A@B@B@@BB@A??@B:B@A??D@>@@?<;;=    BD:Z:IIMLRQPMNLLNMJ@JKIILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKIAKILKNNMIAKLLNNKLJAJJMIJAKIJKIJAAKNMPNPONOKJLLM   MD:Z:100    RG:Z:0  BI:Z:MMPPRRPPPOPPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONQMNHPNNPNNHHPQPRQRPOPLNOPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:42:18843:165751#0  16  chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    1<<A>=>DBCD@CCAAA?EBCDA?D@D@CBA@BCBCCA@A@CCC?DAA?@BBBD@?BE??BBB?BD?7@BB@@D@BBD@BE@BBBDAC??C??><92364    BD:Z:LNMNPONLONLKAKNLKOLKJKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMNNLNNMOOMNJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQQPPPPPRRQPIORQPRQONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHNNQLNHMLNMLNGHNPNOQPPOPPQROQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:47:9508:92109#0    1040    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    %;=A<??C@AD@CCB?A?E@CC@?C@C@CBA@BCCCCAAA@CCB?C???@BCCE@?BD??CCB?BE?7@BCA@E@CCD@?@>BBBD@B>@B>>=<;4353    BD:Z:LNMNPONLONLKAKNLKOLKJKLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMNNLNNMOOMNJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQQPPPPPRRQPIORQPRQONPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHNNQLNHMLNMLNGHNPNOQPPOPPQROQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:28:16767:127812#0  1040    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    .17><9=C@@868=7><@D7BB??B@B=A@A@?=7@A@?3@@@B?9==>@AAAB@>@B=@@@B>AD(6;@BA?B;@?B?AB@ABAD?@>?B<?><=3343    BD:Z:KMMMPONLONLKBJNLKNKJILLLJJJJJLMNKAAAJMIIKAILLLJIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJJMNMMMNNOOMNII   MD:Z:100    RG:Z:0  BI:Z:NPPOOPPORRQPHNRPPQQPOQPQNONONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPQQROPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:46:9810:69065#0    1040    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    1<<?=?>CAB>@CBA@B@DABC@>DAC?CCA?BBBBBA?@?BBB>C@@@ABBBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C??><92364    BD:Z:KMMMPONLONLKBJNLKNKJILLLJJJJJLMNKAAAJMIIKAILLLJIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJJMNMMMNNOOMNII   MD:Z:100    RG:Z:0  BI:Z:NPPOOPPORRQPHNRPPQQPOQPQNONONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPQQROPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:65:11459:169889#0  1040    chr3    58125489    60  100M    *   0   0   CGATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCAT    .9=>=>>CABD@BB?@A@D>BC@>B@C@BBA@BCBBB@?@?BBB?C@@>ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??B??><92364    BD:Z:KMMMPONLONLKBJNLKNKJILLLJJJJJLMNKAAAJMIIKAILLLJIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJJMNMMMNNOOMNII   MD:Z:100    RG:Z:0  BI:Z:NPPOOPPORRQPHNRPPQQPOQPQNONONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPQQROPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:21:7603:28973#0    0   chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTCTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    35569?<=?A@@BC@B?BAAC??>?<=7<<@DBC@CC>C@D?AB??><>?AACA>?B@?D@>B=BA?7ACC@C@@BCAA@AACCCAA>?D@>A@C>;==<    BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJJJIJKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN   MD:Z:34T65  RG:Z:0.1    BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOOONOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:25:10397:24281#0   1024    chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    44638?<??B?@BB@CAC?@C@A>@A?ACB@EBCCBBAD@DBBB>@>>>ACBC@A?C@@DCCB?C@@8@CC@C@ACC@@C@ACCC@A@@EA?AAC@<@=;    BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:46:19268:182386#0  1024    chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    44638?<??B?@BB@CAC?@B@A?@A?ACBAEBCCBBAD@DCBB>@>>>ABCC@@?B@?ECCB?C@@7ACCBD@@CC@AC@ACCC@A@@DA>@AA@<>=;    BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:63:4260:118500#0   1024    chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    4453:?<??B?@BB@CAC?AC?A??A??CB@DBCCBBAD@ECBB>A>>>ABCC?A?@@?CCCB?B@@7ACCAC@ADDAAC@ACCC@A?@D?>@@A=;>=<    BD:Z:JJMNQQNOLMNNJAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIKKJKAAKNLONPONPLLMMMN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSPQQOPQQOHOPNNOPNOPPQNOONOQPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNOOOIIPRQSQRPQQNLOQQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:21:17428:130689#0  16  chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    :=?;=>CBCEACC@AAAEBCCA?EBC@CCA@BCCCCA@AACCB?DAAAACBBD@?AD??BBB>BD?7@CB@@D@BCD?CD@BBBDAC??C@??><73684    BD:Z:NMNOOMMONMKAKNLKOLKJLLKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMNLMNLNOPNMJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPROOORRQPIORQPRQPNPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHONPMNGNLNMLNGGNPNOQPPOPOQQQQQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:24:13055:69026#0   1040    chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    88>9>>A@BD?B@A>@@D@<B=6B>B>6@A@ABBBBA@@@BB@?A>>=>BCCD@>BB?@BBB?AB=7<BBA@C>BBC>BB@AAAB?<<@A==><>:8363    BD:Z:MMMONNLOOLKBKNLKNKJILLLJJJJJLMNKAAAJMIIKAJKLLJJKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJIMNMLMNLONQNMII   MD:Z:100    RG:Z:0  BI:Z:PPOQNPNQRRPHNRPPQQPOQPQONOONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOQRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:44:19183:198872#0  1040    chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    0485::=<?96;@<??;B>7??9=>>=@>A@;AAC@@?9=@2@??=<=><A9B@?@C>?>>7:?@?7<AB@@D>AB@<AC<AAAC?A>??=>?9=87473    BD:Z:MMMONNLOOLKBKNLKNKJILLLJJJJJLMNKAAAJMIIKAJKLLJJKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJIMNMLMNLONQNMII   MD:Z:100    RG:Z:0  BI:Z:PPOQNPNQRRPHNRPPQQPOQPQONOONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOQRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:5:16073:147208#0   1040    chr3    58125490    60  100M    *   0   0   GATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATG    79<:=>C@?C9?B<@>@CABC@?CBB@@AA@?BBCBB@>@BBB?C@>@ABBBD@>BD?@BBB>BC?7@BB@@D@BBB?BD@BBBCAC??A@??>=83574    BD:Z:MMMONNLOOLKBKNLKNKJILLLJJJJJLMNKAAAJMIIKAJKLLJJKLJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJIMNMLMNLONQNMII   MD:Z:100    RG:Z:0  BI:Z:PPOQNPNQRRPHNRPPQQPOQPQONOONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOQRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:3:12278:12956#0    0   chr3    58125491    60  100M    *   0   0   ATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGT    4623<:??A?@BB@CAC?AB@@?@@?<CB@EBCCCBADAEBCB>@>>>ABCD?@8B?@ECBB?C@@8ACCBDAACDA@D?ACCC@BB@EA>ABDA?A=;;    BD:Z:JJNNQOPLNNNKAJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJKJKAAKNLONOONOMLNMMNJ   MD:Z:100    RG:Z:0.1    BI:Z:NNQQQQROPQQOHOPNOOPNOPPPOONOOPPQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONOIIPRQSQRPQROMMQQQN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:43:2048:193931#0   16  chr3    58125491    60  100M    *   0   0   ATGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAGATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGA    >?:<=DABF@CCA?A?EBDD@?BBEA@CAABBCCCA@A@CCC?DA??@C?CDA>BD??C@B?BD?7@CB@@E@BCD@BE@BBBDAC??C@@@?>;86854    BD:Z:MNONMLPNMLAKNLKOLKJLMKJJJJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMMJMKKLMMKJAJJNHJAIHJIHJAAIKJMMMLMMLMNPQMLJJ   MD:Z:59A39T0    RG:Z:0.1    BI:Z:QPRQNNQRQPIORQPRQPOPPPONOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQPOQPPQPPOOHOOPLOGMMNMLNGGMPNOQPPOPOPQPSQPNN   NM:i:2  AS:i:94 XS:i:20
HWI-ST115_0148:1:41:8424:161682#0   0   chr3    58125492    60  100M    *   0   0   TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA    3328:;=@?@CCAD@D@@CAA<?A?9@?;ABBCCC;D@CCCB?@>>=@BCC@A?B@@BCCB<BA?7ACCACAAC@?@B>ACDDA@?>D??@@CAAB>9;:    BD:Z:JJNNOQMNONKBJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJJKAAKNLONONNOLMNNMNJM   MD:Z:100    RG:Z:0.1    BI:Z:NNQORRPPQQOHOPNOPPNOPPPNPNNPPOQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNIIPRQSQRPQRPNNOQQNQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:68:19429:200488#0  1024    chr3    58125492    60  100M    *   0   0   TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA    42377<>A>ABB@CAC?AC@A??A??BA?DBCCCCADAEBCB?@>>>ABBC@??=@?BC>B?BA?7ACB?CAACB@AC@ACDD@A@?>?>A@B@?A>:<:    BD:Z:JJNNOQMNONKBJKJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJJKAAKNLONONNOLMNNMNJM   MD:Z:100    RG:Z:0.1    BI:Z:NNQORRPPQQOHOPNOPPNOPPPNPNNPPOQOHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNIIPRQSQRPQRPNNOQQNQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:42:11358:14661#0   16  chr3    58125492    60  15S85M  *   0   0   GTGCTCTTCCGATCTTGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGA    ;?:;>?BA>7ABA@BD?@>C@BD@BB@?B?EABD@>CBC@BB@?BBBBB@?@?BBB?C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@AC>>;6854    BD:Z:HMOLLLLMNLNMLJIMOMLJMMKJAJNLKNKJILLLJJJJJLLNKAA@ILHHJ@IKKKIIKLJ@HHKKLLMJ@IKKLLLJJ@IJMHKAIILJJNDEIKII   MD:Z:85 RG:Z:0  BI:Z:LOQOMONQOPQQPPOPRQQOQQQPGNQOOPPONPOPNNNONPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLPIINPMM   NM:i:0  AS:i:85 XS:i:19
HWI-ST142_0240:1:47:7919:158863#0   16  chr3    58125492    60  100M    *   0   0   TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA    ?9::A@BD@BB@A@@D@BC@?CACBBCA@BCBBC@?@@BBB>C@@@A?BBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@??<;;8424    BD:Z:MOMLKOOMLBKOMKNKJILLLJJJJJLMNKAAAJMIIKAJLLKJJLMJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMMLLMLMLPQQILII   MD:Z:100    RG:Z:0  BI:Z:OQPPLPPQQINRPPQQPOQPQOOOOOPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPQRRLOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:16954:66540#0   1040    chr3    58125492    60  100M    *   0   0   TGCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTA    :899?=@B>?>6?A?D@BB>>B>=>A9AA?=>,B?8675.A?A>==>7?@D@?@D48416;>D46@BB@@D>BBD@BD<B@?A=?=?A;?@?@<;;8324    BD:Z:MOMLKOOMLBKOMKNKJILLLJJJJJLMNKAAAJMIIKAJLLKJJLMJ@HHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMMLLMLMLPQQILII   MD:Z:100    RG:Z:0  BI:Z:OQPPLPPQQINRPPQQPOQPQOOOOOPPPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPQRRLOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:5:9086:50476#0 0   chr3    58125493    60  100M    *   0   0   GCTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAG    43729<@>?AB@CAC?@B@@>@@@AA@?CABBBB@C@DB?B>@>>>BABB?@>B??BBCB?B@?7ABB@B@ABC?@B@AA@B?A@?DA>>>C@>B@::9;    BD:Z:IIMLQNOONKAKLJILLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKLNNMHAKLLNNKLJAJJMIJAKIJKIJAAKMLOMONMNLLNNNNILM   MD:Z:100    RG:Z:0  BI:Z:MMNPRQRPPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGPMNPNNHHPQPRPQPPRPOOPNPMPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:41:14541:111245#0  0   chr3    58125494    60  100M    *   0   0   CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT    47259>=?AB@CAC?@B@@???@>BB=CABBBBAD@DCBB?@>>>ABBBAA>C@?ECCB?BA?8ACCBC@ACCAAC@ACCC@A@@EA?ABDBACB><:;;    BD:Z:JJLNNPPOLBKLJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJAAKNLONONMNLLNONOJMNN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQQQRQOHOPNOPQOOPPPNONOOPPPNHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHPRQSQRPQRPOPQPONQQQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:43:1724:2639#0 1024    chr3    58125494    60  100M    *   0   0   CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT    3627<><?>B9B;C@=B?@>14<<597>9:8<=5?:AA:?:<<=899C-?>/?;5@6A?<B??7?24;<>;.@0::=?@?C@B??D??@@C>>7B%%%%%    BD:Z:JJLNNPPOLBKLJJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJAAKNLONONMNLLNONOJMNN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQQQRQOHOPNOPQOOPPPNONOOPPPNHHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHPRQSQRPQRPOPQPONQQQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:26:7434:174379#0   1024    chr3    58125494    60  100M    *   0   0   CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT    47249>=?AB@CAC?@B@@>>@>5A?>BAABBBAD@DBBB>@>>>ABBB?@>B??DBAB>B?@7@BCAC@@BC@@B?@BBB?@??D@?@@CA@DA=;9;9    BD:Z:IILMOOQNKBKLJJLLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKNNMI@KLLNNKLJAJJMIJAKIJKIJAAKMLOMNNMNKLNNOOILMN   MD:Z:100    RG:Z:0  BI:Z:MMPPQRRPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGONMPNNHHPQPRPQPPQPPPPPNMPPQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:44:6770:103256#0   1024    chr3    58125494    60  100M    *   0   0   CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT    4624;>=?@A?A<A?@B@???@??BB@DBBBBB@D@DBBB?@>>>AABB?@?A??CABA?B@?7@B?>C?@BB?@B??BB@?A@?D@>@@C@@DA=;9:7    BD:Z:IILMOOQNKBKLJJLLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKNNMI@KLLNNKLJAJJMIJAKIJKIJAAKMLOMNNMNKLNNOOILMN   MD:Z:100    RG:Z:0  BI:Z:MMPPQRRPNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGONMPNNHHPQPRPQPPQPPPPPNMPPQ   NM:i:0  AS:i:100    XS:i:20
HWI-ST142_0240:1:43:4359:117904#0   16  chr3    58125494    60  100M    *   0   0   CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGA    6(;:>C@BB@@A@C@BC@?=AC@BB@@CBCBB@@@?BCB>C@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@<>@@92754    BD:Z:MLJMNMLCLOMLOKJILLLJJJJJLMNKAAAJMIIKAJLLLJILMKAHHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKLMKLLNOPLPLJII   MD:Z:99T0   RG:Z:0  BI:Z:PPNPNPOHOSPPQQPOQPQOOOPOPQPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPRNQPNMM   NM:i:1  AS:i:99 XS:i:20
HWI-ST142_0240:1:48:9513:187557#0   1040    chr3    58125494    60  100M    *   0   0   CTAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGT    99=;@B@BB?@@@C@BC@>C@CABCA@BBBCB@@@?BCB>B@@@ABBBD@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@=>@@92744    BD:Z:MLJMNMLCLOMLOKJILLLJJJJJLMNKAAAJMIIKAJLLLJILMKAHHKKLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKLMKLLNOPLPLLII   MD:Z:100    RG:Z:0  BI:Z:PPNPNPOHOSPPQQPOQPQOOOPOPQPPGGGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPRNQPPMM   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:44:1737:13754#0    0   chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCGGGTCTCCATGTAGTG    3244<;>AA@DAC?@B>?>AA>ACBAEBCCBBAE@DBCB>@>>>ABBB?A?B?@DBBB?BA@8ACCBD@ACC@A@A@CCC@AB>8>>@6D@?DB>>;9<;    BD:Z:JJNKPQPLCKLKJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@AKNLOLMJMNKLNNOOKMNNI   MD:Z:84A15  RG:Z:0.1    BI:Z:NNQORRROHOPNOPQOPPPPNONNPPOQNGHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHORQSOPKQRPOPRQPLQQQM   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:44:17666:60002#0   1024    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    4245;;>AA@CAC?@BA?>@A??CB@EBCCBBAD@DBCB>@>>>@BBB?@?B@@DCCC>C@?8ACCBC@@CC?AC@ACCC@AB@FA?A@D@ADBA?;;<<    BD:Z:JJNKPQPLCKLKJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@AKNLONONMNKLNNOOKMNNI   MD:Z:100    RG:Z:0.1    BI:Z:NNQORRROHOPNOPQOPPPPNONNPPOQNGHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHORQSQRPQRPOPRQPLQQQM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:67:11815:169115#0  1024    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    3226<<>A<?B?B@AB>>?@>9;>9=C@>>@C@;?DCCB>?<<<A??<=>?B@?C@AA<B@?6A@B@CAA;@A>=?>A?<@@??D@=@@C@>EB@%%%%%    BD:Z:JJNKPQPLCKLKJMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@AKNLONONMNKLNNOOKMNNI   MD:Z:100    RG:Z:0.1    BI:Z:NNQORRROHOPNOPQOPPPPNONNPPOQNGHHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHORQSQRPQRPOPRQPLQQQM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:45:19953:130237#0  1024    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    4244;;>AA@CAC?@B@@>@@>=A@@DBBBBBAD@DBBB>@>>>BBBB?@>B??DCBB?B??8@BBBC?@CC@AB@@BBB?A@?D@>AAAA@DB>>9:;:    BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI   MD:Z:100    RG:Z:0  BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:63:9832:33189#0    1024    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    4244;;>AA@CAC?@B@@>@>>9??=BAAABB@D@DBBB>@>>>ABBB?@>B??DBBA>B??7@BB@C?@BB@@B@@BBB?A@?D@>A@CA@DB>>9;:9    BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI   MD:Z:100    RG:Z:0  BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:67:3073:131364#0   1024    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    4234;<>AA@CAC?@B@@>@?=:?8?BABBBBAD@DBBB>@=>>BBBB?@>B??BACB>B??7ABBBB?@BB?@B@@BBB;@@?D@>A@C@@D@>>::;9    BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI   MD:Z:100    RG:Z:0  BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:6:19159:38857#0    1024    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    4244;;>AA@CAC?@B@@>@@@@BBADBBBBBAD@DBCB>@>>>@BBB?@>B?<DBBB>B??7@BBBC?ABB?AB?@BBB=@@?D@>AAAA@DB>=9;;:    BD:Z:IIMKPQPKBLLJJMLIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMNMIAJLLNNKLJAJJMIJAKIJKIJAAKMLOMNMMNKKNNOPJLMNI   MD:Z:100    RG:Z:0  BI:Z:MMPORRRNGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMNONNHHPQPRPQPPQOPQQPPKPPQM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:21:4682:56360#0    16  chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTA    9><>CABCAAA?EBCDA?EBDBCCAABCCBCA@@ACCC?DAAA@CCCE@?BD??CBB?BD?7@BB@@D@BBD@BD@BCBDAC??C@@@@@>>AB<77424    BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLMJJ   MD:Z:99G0   RG:Z:0.1    BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPPNN   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:27:11042:76831#0   1040    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    :>9>DACC>@@@E@CC@.C@D@@CA@BB@BBAA@@CC@?CA>?@BCCE@?BD??BCB?BE?7@BB@@E@BBD@>D@BBBD@C?>CAA@>@>@@B<97484    BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLIJJ   MD:Z:100    RG:Z:0.1    BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPMNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:64:15606:175538#0  1040    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTG    :?;>D@CB@AA?EBCCA?EBDBCCA@CCCCCA@@@CCC?DAA?BCCCE@?BD??BBB>BD?7@BB@@E@BBD@BD@BBBDAC??C@@@@@>>AB<77484    BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLIJJ   MD:Z:100    RG:Z:0.1    BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPMNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:65:18444:50475#0   1040    chr3    58125495    60  100M    *   0   0   TAGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTA    :=<>DABCA@A?EBCDA?DBD@CBBABCBCCA@@ACCC?DAAABCCCE@?BD??BBB>BD?7@BB@@D@CBD@@D@BBBDAC??C@@@@@>>AB<77424    BD:Z:LKNMMLCLOMKOLKJLMLKKKJJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJLLMONKPOLMJJ   MD:Z:99G0   RG:Z:0.1    BI:Z:POQQNNHORQPRQPOQQQPNOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHMLOMLOGGMOMNPPPOPOPPOQQNQRPPNN   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:24:8906:58142#0    0   chr3    58125496    60  100M    *   0   0   AGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG    34468<@A?CAC?AB@@>@A?AAA>EBCCCCAD@EBBB>?>>>@BCCAA>B??ECCB?CA>8ACC@CAACCAAB@@CCC@A??D@?ABCB?EC?A=<;;8    BD:Z:JJKMQQMCLLKKMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@KNLONONMNKKNNNPKNNNIM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPSRPHOPNOPQOPQPPNONNOQOPOGGHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQQSQRPQRPOPRRQMOQQMO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:7:12620:40059#0    1024    chr3    58125496    60  100M    *   0   0   TGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGA    43468<@A?CAC?@B@@>@@?ACB@EBCCCBAD@DBBB?@>>>ABBB?@?B?@ECCC?C@@8@CCBD@@CC@AC@@CCC@A@>E@>ABDBAEC?@>;;<<    BD:Z:JJNMQQMCLLKKMMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@KNLONONMNKKNNNPKNNNIN   MD:Z:0A98G0 RG:Z:0.1    BI:Z:NNQPSRPHOPNOPQOPQPPNONNOQOPOGGHOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQQSQRPQRPOPRRQMOQQMQ   NM:i:2  AS:i:98 XS:i:19
HWI-ST142_0240:1:42:11406:175267#0  1024    chr3    58125496    60  100M    *   0   0   TGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG    43468<@A?CAC?@A@@>@@>=A>=DBBBBBAD@DBBB>@>>>A?BB?@>B??DBBB>B?>7@BBAD?@CC@AC?ABBB@>@?D@>@BCA@CB??<;;:5    BD:Z:IIMLRPMBLMJJMMIIMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMMIAKKLNNKLJAJJMIJAKIJKIJAAKMLOMNMLNKKMNOPKMMNIM   MD:Z:0A99   RG:Z:0  BI:Z:MMPPRRPGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMPMNHHPQPRPQPPQOOQRQPMNPQMO   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:64:3826:31604#0    1024    chr3    58125496    60  100M    *   0   0   AGATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGCGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG    4336:<?A>A?C?@@@=?@A=:@<=CAA?AB?D@DBB@?@=(3;=@@@?>A??DBCB?B@?8@@A?CA?@B@AB@@BBB?>@?D?>@@1<=B@>?<:::5    BD:Z:IIKLRPMBLMJJMMIIMKLJKJIJLJLI@@@IJGHH@JKLLLJLKI@JHKKMMMIAKKLNNKLJAJJMIJAKIJKIJAAKMLOMNMLNKKMNOPKMMNIM   MD:Z:41G58  RG:Z:0  BI:Z:MMOPRRPGNPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOOMPPNGOMPPPPONGPOPPPMOMGONPMMGOMMPMNHHPQPRPQPPQOOQRQPMNPQMO   NM:i:1  AS:i:95 XS:i:20
HWI-ST115_0148:1:24:12153:162085#0  0   chr3    58125497    60  100M    *   0   0   GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGCTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA    3556=?@>??@??C?=;:<;2>95=;=@=:?C@DCCB?@=?>=BB?@=:=A?DCCB?BA?7>=C@C@?AC@@@@>=?B>?9=B?<B5B<=?@@@>>=;%%    BD:Z:JJMNQNDLMKKNMJJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMMMIJIJJIJ@@JNLONONMNKKMNNOLNONIML   MD:Z:67T32  RG:Z:0.1    BI:Z:NNPQSPIOPNOPQOPQQPNONNOPPPNHGGOPMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPQONONNONNHHOQPSQRPQRPOPRRRNPOQMOP   NM:i:1  AS:i:95 XS:i:20
HWI-ST115_0148:1:24:7479:25230#0    16  chr3    58125497    60  100M    *   0   0   GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA    :<@;AC3?B?D@CC@@CBC@B@?@=A3@A@B;@CBB?A<=AABCCE@?CE?@ACB?BD=8>CCA?C@CBDACB@CCCB@@??B>?<?A=@=B><?::233    BD:Z:NMLKBLPMLPLKJLMLKKKKKLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKMNMJNNOLMMJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPOFMQQPRQPOQQQPOPOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMNMMNGHMOMNPOOOPOPPOQQMPQQOQONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:27:11695:17911#0   1040    chr3    58125497    60  100M    *   0   0   GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA    :=@=BB??A@EBCDA@D@C@CCA@BCCCCA@AACCC?CAAABCCCEA?BE??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BC?<?98454    BD:Z:NMLKBLPMLPLKJLMLKKKKKLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKMNMJNNOLMMJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPOFMQQPRQPOQQQPOPOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMNMMNGHMOMNPOOOPOPPOQQMPQQOQONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:46:6962:183922#0   1040    chr3    58125497    60  100M    *   0   0   GATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGA    ;=@<BCAAAAE@CDA?DBC@CBA@BCCCCA@@ACCB?DAAA@BCCDA?BE??BBB>BD?7@BB@@E@BBD@BD@BBBDAC??C@@@@@>?BC?<?98454    BD:Z:NMLKBLPMLPLKJLMLKKKKKLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKMNMJNNOLMMJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPOFMQQPRQPOQQQPOPOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMNMMNGHMOMNPOOOPOPPOQQMPQQOQONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:48:17649:64283#0   0   chr3    58125498    60  100M    *   0   0   ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG    3536<>=?<A=@A6?<;>:>;76:1737+3C<BCC>552<;7>>@@;>?=>BA<@?=@?4@?@?B@<9>?::59:@B@A?7D?;@@A>>BA@@?>><7%%    BD:Z:IINMNDNMKKMMJJMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLHAKLLMNKLJAJJMIJAKIJKIJAAKMLOMNMLMJKMMNPKNOOIMKL   MD:Z:100    RG:Z:0  BI:Z:MMPPPIPPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMNGHPQPRPQPPQOOPQRRNPPOMOPO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:65:18859:157756#0  1024    chr3    58125498    60  100M    *   0   0   ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG    3539<=<A>A=AB5====6=<<9<;??AA;C?CBBB?@=<?=B?B@@??A?DB@B<?6>52;;5=AABB@;6><@??=@?<D@>A@@:>?AA>=?%%%%%    BD:Z:IINMNDNMKKMMJJMKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLHAKLLMNKLJAJJMIJAKIJKIJAAKMLOMNMLMJKMMNPKNOOIMKL   MD:Z:100    RG:Z:0  BI:Z:MMPPPIPPNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMNGHPQPRPQPPQOOPQRRNPPOMOPO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:3:13191:78850#0    16  chr3    58125498    60  100M    *   0   0   ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG    >@;?B?@@@DBCCA?CBEBCCA@CC@CCAAA@BCA?D??ABCCCDA?CD@?CCB?BD?7@CCAAC@BBD@AD@BBBDAC??CA@@@A=?BD?=A<=4574    BD:Z:MLKALONLPMKJLMLKKKKKMMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLNMINMNLPMKJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPOHLPPPRQPOQQQPOPPOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMOMLOGGNOMNPOONPOPPOQQMPPQNSONNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:7:9375:185965#0    1040    chr3    58125498    60  100M    *   0   0   ATGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAG    =?<=@@@@@D@BC@?C@C@BB@?BCBCCA@@?BBB>CA@>ABBBD@>BD?@BBB=BD?7@BBA@D@BBD@BD@BBBDAC??C@@@@@>?BD?>A<>4574    BD:Z:MKJAKPNMPLKJMLLJJJJJLMNKAAAJMIIKAJLLLJJLMK@IILLLLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKKMNMJMMOKPLJII   MD:Z:100    RG:Z:0  BI:Z:PPOGKQOPRRPOQPQOOOPOQQQQGHGNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPRNRONMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:22:8056:180239#0   0   chr3    58125499    60  100M    *   0   0   TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG    4346;<A@B?@BAA>A@AACB@DBCBBBAD@DBCB?@>>>ABBCA@?B??ECCB?C@@8ACCBD@@CC@AC@@CCC@A@@EA?A@CBAEC?@?@A@:<:9    BD:Z:JJNKENNLLNNKJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKONONMNKKMMMOKNPOJMLLN   MD:Z:100    RG:Z:0.1    BI:Z:NNQOJPQNOPQOPQQQOONNOPOPOGGHNOMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPRPQRPOPRRRORQPKOPPO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:26:13604:44997#0   1024    chr3    58125499    60  100M    *   0   0   TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG    4346=>A@B??CA@?>@>?CA?BABBBBAD@DBCB?A>>>BACD?A?B@@DACC?CA@8ACCBC@ABC?AC?ACCC@AB@FA>ABDBAECAA???>;<:8    BD:Z:JJNKENNLLNNKJMLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKONONMNKKMMMOKNPOJMLLN   MD:Z:100    RG:Z:0.1    BI:Z:NNQOJPQNOPQOPQQQOONNOPOPOGGHNOMMNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPRPQRPOPRRRORQPKOPPO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:25:6242:39043#0    1024    chr3    58125499    60  100M    *   0   0   TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG    4346;<A@B?@B@@>>@>:AA@CAABBBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBBC?@CB?AC@ACBC?@@?D@>@ABA>DB?@?@?=9;;9    BD:Z:IIMJENOKKNMJJNKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@KLLNMKLJAJJMIJAKIJKIJAAKMLOMNMLMJJMMNOKNOPJMKLM   MD:Z:100    RG:Z:0  BI:Z:MMPNIPRNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMHGPQPRPQPPQOOPQQROQPQKOPOO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:6:7030:191659#0    1024    chr3    58125499    60  100M    *   0   0   TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG    2347<;>>:?@A>;;<?=6??;A<>AAB@C=CBBB?>>>=A@BB@@?BA?CBCB>>@>6=@@>A?@B@@AB@AAB@?@??A?>@@B@>CB@@>@?=::85    BD:Z:IIMJENOKKNMJJNKLJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@KLLNMKLJAJJMIJAKIJKIJAAKMLOMNMLMJJMMNOKNOPJMKLM   MD:Z:100    RG:Z:0  BI:Z:MMPNIPRNNPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMHGPQPRPQPPQOOPQQROQPQKOPOO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:61:19340:26239#0   16  chr3    58125499    60  3S97M   *   0   0   ATCTGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGG    ;;9?;;84;<>C@BC@?E?E?AA@@ACA<C@??<;@<?C>><@BCAC<?BE?AC?B>BE=5>CC??C>ACD@<C@CAAE@B>?B>?A?==?AB=;>4613    BD:Z:MKOLLBMOMLOLKJLMLKKKKJLMMKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKLMNNJOOOIMJJ   MD:Z:97 RG:Z:0.1    BI:Z:POQPMGNRQPRQPOQQQPOOOOPQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNLNNLNHGMOMNPOPOPOPPOQQMQQRMQNN   NM:i:0  AS:i:97 XS:i:20
HWI-ST142_0240:1:22:5430:189914#0   16  chr3    58125499    60  100M    *   0   0   TGAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGG    ?<===?A@D@BCA>CABABB@?BBCBC@@@@BBC>C@A@ABBBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@=B>@95744    BD:Z:KJAJONMPMKJMMLJJJJJLMNKAAAJMIIKAJLLLJJLMKAHILLMLMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJMNMIMMMKOPJLII   MD:Z:100    RG:Z:0  BI:Z:POGMOOOQRQOQPQOOOPOQQQQHGHNPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPNRQNPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:25:7867:50214#0    16  chr3    58125500    60  100M    *   0   0   GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT    ;>>=@A?DBCCA?E@DBCCA@CCCCCA@A@CBC?DAA?BBCCEA?CE??CBB>BD?7@BB@@D@CBD@BD@BBBDAC??C@@@@@>?BD@>B?B<:7444    BD:Z:KAKNMLQMLKLMLKKKKKMNNKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLIMLMJOPNLMJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHNQNNQQPOQQQPOPPPQQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMNGHMONNPOONONPPOQQMPPPMRPPPONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:6:13768:9775#0 1040    chr3    58125500    60  100M    *   0   0   GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT    ;><>@A@EBCDA?CBE?CCA@CCCCCA@AACCB?DA?A@CCCEA?CE@?CBB?BD?7@CB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>B?B<:7444    BD:Z:KAKNMLQMLKLMLKKKKKMNNKAAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLIMLMJOPNLMJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHNQNNQQPOQQQPOPPPQQQPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMNGHMONNPOONONPPOQQMPPPMRPPPONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:24:7977:30681#0    1040    chr3    58125500    60  100M    *   0   0   GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT    79:<<?<@>;=@?B@B@BA>@ACAB7A@@@896><>>>@ABBC@?BD>??AB?BD>7@BB?9A8=@D?AB?BBBA@?>@B>;@?@>?<A<><<A::7123    BD:Z:JAJNMMPMLJMMMJJJJJLMNKAAAJMIIKAJLLLJJLMKAIHLLMMMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLNMILMMIOONLLII   MD:Z:100    RG:Z:0  BI:Z:OGMQMOPQQPQPQOOOPOQQQQHHGOPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLRQPPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:67:7724:174315#0   1040    chr3    58125500    60  100M    *   0   0   GAAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGT    6:::<@@D@BC@?C@B@BB>?@CBBAB@@@BBA?A>>>@AABD@?AC?@BBA>AA=5@BBA>C?BBD@BC@ABBD@A??A>@@?@=?AD?>B?A::8134    BD:Z:JAJNMMPMLJMMMJJJJJLMNKAAAJMIIKAJLLLJJLMKAIHLLMMMJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLNMILMMIOONLLII   MD:Z:100    RG:Z:0  BI:Z:OGMQMOPQQPQPQOOOPOQQQQHHGOPMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLRQPPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:64:14210:42818#0   0   chr3    58125501    60  100M    *   0   0   AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG    4664<=A>?B@@>@@>?BA@DBCCCB@D@ECBB>@>>>ABBBA@>B??BBBB?BA?8@ACBC?ACCAAC@ABC@@A@?D?>@@C@?EB?A??A?><<%%%    BD:Z:JJBKONMOOKKNLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMONMNKKMMMNJNOOKNMLNMI   MD:Z:100    RG:Z:0.1    BI:Z:NNHOROPPQOPQQQOPONOPOPNGHGNPLLNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOQRPOPRRRORRRMNNPOPM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:68:20373:148127#0  1024    chr3    58125501    60  100M    *   0   0   AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG    4664<=A>?B@@>@@>>BA@DBCCCB@D@DCCB?A>>>ABBC?@?B??BCCB?B@?8@CC@D?A<BAAA@AC-C@>?>??>A@C@>=BA@?B@?<@<78;    BD:Z:JJBKONMOOKKNLMJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMONMNKKMMMNJNOOKNMLNMI   MD:Z:100    RG:Z:0.1    BI:Z:NNHOROPPQOPQQQOPONOPOPNGHGNPLLNHPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOQRPOPRRRORRRMNNPOPM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:45:11826:198924#0  1024    chr3    58125501    60  100M    *   0   0   AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG    4664<=A>?B@@>@@??BA?DBBBBBAD@DBBB>@>>>@BBB@A?B??DBBB>B@?7@BBAC@@BB@@B@@BAC@A@?D@>@ACA@DA?@?@A@>?:8*8    BD:Z:IIAKPMMNNKJNLMJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKLNNKKJAJJMIJAKIJKIJAAKMLOMNMLMJJLLNOJMOPKOLLMLI   MD:Z:100    RG:Z:0  BI:Z:MMGNRPPPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGPPPRPQPPQOOPQQQNRRRMONOOOM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:45:5304:83561#0    1024    chr3    58125501    60  100M    *   0   0   AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG    4664<=A>?B@@>@@@ABB@DBBBBBAA@DBBB?@>>>ABBB?@>B??CBBB?B@?7@BB@B@@BB@AB@ABBB@A@?D@>@ACA@DC?@>@@?=?;8*9    BD:Z:IIAKPMMNNKJNLMJKJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKLNNKKJAJJMIJAKIJKIJAAKMLOMNMLMJJLLNOJMOPKOLLMLI   MD:Z:100    RG:Z:0  BI:Z:MMGNRPPPPNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGPPPRPQPPQOOPQQQNRRRMONOOOM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:1:7700:156580#0    16  chr3    58125501    60  100M    *   0   0   AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG    ;<9;??DABC@>D@C@BBA?BBBBC@?@@CCB>D@@@B@BBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C?C>=<4484    BD:Z:AJNLLPMLKMMMKJJJJLMNKAAAJMIIKAJLLLJJLMKAIIKLMMNJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMMILLMIMOMPLHII   MD:Z:100    RG:Z:0  BI:Z:GMQOMPPPPRPQOOOPOQQQQHHHNQMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPQPROLMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:22:11100:183122#0  1040    chr3    58125501    60  100M    *   0   0   AAACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTG    5.:;??D@BA>=C>C>B?@5@BBBB>;8>4B@?B>8>@?@CD@=BB:?<<A?AC=7@BBA?C=BBA@BA?BABC?A>?A==6=A<@<C>>A>B;<<3373    BD:Z:AJNLLPMLKMMMKJJJJLMNKAAAJMIIKAJLLLJJLMKAIIKLMMNJ@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMMILLMIMOMPLHII   MD:Z:100    RG:Z:0  BI:Z:GMQOMPPPPRPQOOOPOQQQQHHHNQMMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPQPROLMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:43:19528:142803#0  16  chr3    58125502    60  100M    *   0   0   AACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGA    =<;=@DACCA?D@D@CBA@BCCCCA@A@CCC?DAA?@CCCEA?CD??CBC>BD?7@BB@@D@BBD@BD@CBBEAC??C@@A@@>?BE@>C@C???94854    BD:Z:KNLKPMMKMNLKKKKKMNNLBAAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLLLINNMOPILJJ   MD:Z:100    RG:Z:0.1    BI:Z:NQPOOOOOQQQPOPPPQRRPHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHGMPMNQOONONOOOQQMPPPMQOOQQMPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:21:20040:121958#0  1040    chr3    58125502    60  100M    *   0   0   AACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGA    869==CA@C@>CBB@@BA?BBBBBA@@?BBC>C@@@ACBBD@?BD??B@B>BD?8@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@C???94854    BD:Z:JNLKOMLKNMMKKJJJLMNKAAAJMIIKAJLLLJJLMKAIILKMMNK@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLILLLIMMMOPHKII   MD:Z:100    RG:Z:0  BI:Z:MQOONPOORQQOOOPOQQQQHHHOPNMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPOPRQLPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:8:13725:143226#0   1040    chr3    58125502    60  100M    *   0   0   AACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGA    %%%%%%%%A=50>C@BB@@9BBBBA@A@BBA?>@>?>BBBD@?BD??BBB?BD?8@BBA@C@ABD@AD@BBBD@@=?C@@A@@>?BC;?B?C?>?94734    BD:Z:JNLKOMLKNMMKKJJJLMNKAAAJMIIKAJLLLJJLMKAIILKMMNK@IKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLILLLIMMMOPHKII   MD:Z:100    RG:Z:0  BI:Z:MQOONPOORQQOOOPOQQQQHHHOPNMOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPOPRQLPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:46:2641:21330#0    0   chr3    58125503    60  100M    *   0   0   ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC    4475<;?A?@>?@@?BA@DBCBCBAD@ECCB>A>>>ABBB?@>B??DCCB?B@@7ACC@C@ACC?ACAABCC@B?=E@?A@D@AEC@@?AA@?@?%%%%%    BD:Z:JJLKNQPLLNMNJKJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMMNKKMMMNJMNOJNNMOMINM   MD:Z:100    RG:Z:0.1    BI:Z:NNPNQQROPQQQOPOOPPOPNGGGOOLMMGPPQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQPOPRRRORRRNPPOMPMQP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:25:12209:32246#0   1024    chr3    58125503    60  100M    *   0   0   ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC    4365<;>A?>=?@@?A>=@=ABBB@D@CBBB??>7>?ABB???A??=BBB?B??8ABB@B@@BB@@B@ABBB?B??D@>@>C@@DBA??@@?>A?<2:56    BD:Z:IILJNPPKKOLMKLJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMNKLJ@JJMIJAKIJKIJAAKMLOMNMLMJJLLMNJMNOKOMNNLIMM   MD:Z:100    RG:Z:0  BI:Z:MMPNPRRNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPPQPQPPQOOPQQQNQQSOPPOMOMPP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:48:11161:156653#0  1024    chr3    58125503    60  100M    *   0   0   ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC    4475<;>A??>?@?>BB?DBBBBB@D@DBBB?@>>>ABBB?@>B??CBBB>B??7@BBAC?@CB?@C?@CBC@A@?D@>@ACA@CB?@=@@?>@?<<986    BD:Z:IILJNPPKKOLMKLJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMNKLJ@JJMIJAKIJKIJAAKMLOMNMLMJJLLMNJMNOKOMNNLIMM   MD:Z:100    RG:Z:0  BI:Z:MMPNPRRNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPPQPQPPQOOPQQQNQQSOPPOMOMPP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:5:13378:186738#0   1024    chr3    58125503    60  100M    *   0   0   TCTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC    3256<:9?==<<@:=@;=@????8=98>?6<9<=<2,364347/==A7@@?A?>7==?=??<2;=948?@BB@A>:@><82A?<@BA?=%%%%%%%%%%%    BD:Z:IIJJNPPKKOLMKLJIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMNKLJ@JJMIJAKIJKIJAAKMLOMNMLMJJLLMNJMNOKOMNNLIMM   MD:Z:0A99   RG:Z:0  BI:Z:MMNNPRRNOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPPQPQPPQOOPQQQNQQSOPPOMOMPP   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:25:4411:84732#0    16  chr3    58125503    60  100M    *   0   0   ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC    <;;=DACCA?DBCBCCA@BCCCDA@B@CCB?DAAABCCCEA?CE@@CCB?BD?7@BB@@D@BCE@CD@CBBDAC??C@@@@@>?BD@>C@D?@A<99544    BD:Z:NLKOMLLMNMKKKKKMNNLBBAJMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKLIMNLNPLLNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPOQNNNQQQPOPPPQRRQHHHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHMONNPPONONOONQQMPPPMQONQPOPQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:48:8985:65471#0    1040    chr3    58125503    60  100M    *   0   0   ACTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAA    %%97A:@94>74?.-?A6@-B@7A?A8B6B?C>>>??@BB=>4>::@@?>?@93*<A=3B8ACC<AB@BBBA=@89?:=@0(,8-@5;6<@=>?9::353    BD:Z:NLKNLLKNNMKKKJJLMNKAAAJMIIKAJLLLJJLMKAIILLLMNKAIKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHLLLHMMKOOLKJII   MD:Z:99C0   RG:Z:0  BI:Z:QOOPNONQQROOOPOQQQQHHHOQMNOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONRQNPOMM   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:5:9908:10539#0 1040    chr3    58125503    60  100M    *   0   0   ACTCTGAAGTAGAGAATCGTTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGAC    7;;;@@BC@>CAA@BB@7@@ABB@??@BBA?B@>?@BABD@>BD?@B@B?BD?8@BBA@D@BBD@BD@BBBAAB??A@@@@@>?BD@>C@C?@A<99344    BD:Z:NLKNLLKNNMKKKJJLMKKAAAJMIIKAJLLLJJLMKAIILLLMNKAIKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHLLLHMMKOOLKMII   MD:Z:18A81  RG:Z:0  BI:Z:QOOPNONQQROOOPOQQOOHHHOQMNOGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONRQNPPMM   NM:i:1  AS:i:95 XS:i:20
HWI-ST142_0240:1:48:10959:184359#0  0   chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    3538:<?=>=<>97@A;AAAAA>,C;;A=;><===?@2@:=7?>>7<??9B?>5ABB@@??;@?@:>@@@/8A6<B==@?0>=B@@??=<@;@@;>;;%%    BD:Z:IIJJQPMKOMMKLKIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMKLJAIJMIJAKIJKIJAAKMLOMNMLMJJLLMNIMNOJOMNOMIMMI   MD:Z:100    RG:Z:0  BI:Z:MMNNRRPOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOROQPPQOOPQQQNQQROQQOOMMPPL   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:68:13457:188269#0  1024    chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAATTATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACA    3638:=?=?==<<<>./3=166*%85266.3466/?;:4:41161BB/@6?5,6=9@<A;@%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:IIJJQPMKOMMKLKIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLHJIIKMMKLJAIJMIJAKIJKIJAAKMLOMNMLMJJLLMNIMNOJOMNOKJKMI   MD:Z:47A47T4    RG:Z:0  BI:Z:MMNNRRPOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONPNPPPPMOMGONPMMGOMMOMMGGOPOROQPPQOOPQQQNQQROQQOOHJPPL   NM:i:2  AS:i:90 XS:i:20
HWI-ST142_0240:1:7:15934:30090#0    1024    chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    47578<@??>?@@=BB@DBBBBBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BB@C?@@B?@B@@BCC@A@?D@>@@CA@DB?@??@??A@=>;;7:    BD:Z:IIJJQPMKOMMKLKIJLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMKLJAIJMIJAKIJKIJAAKMLOMNMLMJJLLMNIMNOJOMNOMIMMI   MD:Z:100    RG:Z:0  BI:Z:MMNNRRPOQPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOROQPPQOOPQQQNQQROQQOOMMPPL   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:45:11810:80190#0   16  chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    ;:;??AC@>CAA@BB@?BBBCB@?@?BBB?D@@AABBBD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@>>?BD@>C@D@@B><=5434    BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII   MD:Z:100    RG:Z:0  BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:4:11059:91320#0    1040    chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    9;:??AC??CAB@BB@?BBBBB@?@?BCC?D@>A@BBCD@>BD??BBB>BD?8@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@@B><=5434    BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII   MD:Z:100    RG:Z:0  BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:66:2424:23337#0    1040    chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    ;::??AA@?C@C@BBB?BBBBB@???CCC?D@>AABBBD@>BD??BBA>BD?7@BB@@D@BBD@BD@BBBD@C?@C@@@@@=?BD@>C@D@@B><=5234    BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII   MD:Z:100    RG:Z:0  BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:6:16257:60848#0    1040    chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    9:8??AA@?C@C@BBA?BBBABA@@?BB@?B>>4@=BCD@?BD??BBB?BD?8@BB@@D?CBD@BC@BBBD@<??B@@@?=>?AD@?C@C@>B><=5434    BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII   MD:Z:100    RG:Z:0  BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:7:7721:186507#0    1040    chr3    58125504    60  100M    *   0   0   CTCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACA    ;::??AC@>CACBBB@?BBBBB@?A?CCC?D@>A@BBCD@>BD??BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@A>?BD@>C@D@@B><=5434    BD:Z:LKNKKKNNNKKKKJLMNKAAAJMIIKAJLLLJJLMKAIILLMLNKAJKKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKLLHLMKMOKOMIII   MD:Z:100    RG:Z:0  BI:Z:OOPPMNPPRPOOPOQQQQHHHOQNMPGNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPQNRPMMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:41:11840:25955#0   0   chr3    58125505    60  100M    *   0   0   TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACAT    45739>>?=@@AACBAEB==BBAD@DBBB>@>>>ABBB?@>B??DCBB>BA?8ACCBC@@CC@AC@ACCC@AA@E@?ABCBBEC@A?AA@>B@?%%%%%%    BD:Z:JJKNQNMONNKLJJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMKKMMMNJMNNINMMPKKLMIM   MD:Z:94T5   RG:Z:0.1    BI:Z:NNOPSPQQQQOPOOPQPPNGGGNOMLMHOOQOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONPRRRORRRNPQQOIHPPMQ   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:42:14945:88827#0   16  chr3    58125505    60  100M    *   0   0   TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAA    <9>;BD@?CAD>CCA@BCCCCAAA@CCC?CA?ABBBCCA?CE?@BCB?@D?7@CC@@D@BCD@CE@BBBE@C?@C?@A@@>@BD@>C?D@AB?>@:5364    BD:Z:KOLKKMOMLLKKKMNNLBBBKMHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHMMKMNKOQJKJJ   MD:Z:99T0   RG:Z:0.1    BI:Z:OQPOLOPQPOPPPQRRQIIHOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPMNQOOOONOONPPMPPPMQONPONQSNONN   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:43:3535:86981#0    1040    chr3    58125505    60  100M    *   0   0   TATGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAT    7-<:;?868959):=:?AA(@A?7?BA@?C><;?BBBD??BD>?B?B?BD?8@BC@@D:?CB:@@<?CBA<C?>A@@@?@=?AB?>=>B=>=99?;6353    BD:Z:LMKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKNQINII   MD:Z:1C98   RG:Z:0  BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONRRMPMM   NM:i:1  AS:i:98 XS:i:0
HWI-ST142_0240:1:4:15304:147199#0   1040    chr3    58125505    60  100M    *   0   0   TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAT    8:?<@B@?CAC@BBA?BBBBB@?@@BBB?DA@@@BBBE@>BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@AB?>@:4364    BD:Z:KNKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKNQINII   MD:Z:100    RG:Z:0  BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONRRMPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:62:21314:22111#0   1040    chr3    58125505    60  100M    *   0   0   TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGNCAT    88=;?B9?B@;@BB@@BCB8BA?@?BB@?C>?=?BBBD@?BD??BBB?BD?7@BB@@D@BAC?BB@BBBD@C?@A@@@@@>?BD@?C@D?=@;6>:%432    BD:Z:KNKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKLMINII   MD:Z:96A3   RG:Z:0  BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONOOMPMM   NM:i:1  AS:i:98 XS:i:19
HWI-ST142_0240:1:68:17222:120327#0  1040    chr3    58125505    60  100M    *   0   0   TCTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACAT    9;?<@B@>BAC@B@@@A@B=BA@@@;A@?A>@>@BBBE?>BD?ABBB>BD?7@BBA@D?ABD@BC@BBBD@B??B<@@@>>?AC@<C@D@AB?>@:4354    BD:Z:KNKJJNNNLKKKKLMNKAAAJMIIKAJLLLJJLMKAIILLMMMKAJLKLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKLHLLKMMKNQINII   MD:Z:100    RG:Z:0  BI:Z:OPPOLPOQPPOPOQQQQHHHOQNNOHNPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPONRRMPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:23:6412:139348#0   0   chr3    58125506    60  100M    *   0   0   CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGCGCCATA    3637<;><7@17/0-34.>??88?A@>>><=>?7B>;:7;;:979>>.=7:4=?<=<@A35<?<<>B4:=-815>0A>@=>B=A%%%%%%%%%%%%%%%%    BD:Z:JJNNNNPNOKLKJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJKMMMNJMNNIMMMOPMONMML   MD:Z:93T1A4 RG:Z:0.1    BI:Z:NNPQQQRQQOPOOPQPQNGGGNOLMMGPOPOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONORRRORRRNPQQPOMNPQQP   NM:i:2  AS:i:93 XS:i:19
HWI-ST115_0148:1:28:17969:176762#0  1024    chr3    58125506    60  100M    *   0   0   CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATA    3735<>=>@@>;>?>BABBAC@D@EC>B?@??>:ABAA=>A@=BACB?B@?7@C@?@@?@CA?=@?B@9>B@?D@9A%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJNNNNPNOKLKJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJKMMMNJMNNIMMMOLKMMIML   MD:Z:93T6   RG:Z:0.1    BI:Z:NNPQQQRQQOPOOPQPQNGGGNOLMMGPOPOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONORRRORRRNPQQPJINPMQP   NM:i:1  AS:i:95 XS:i:20
HWI-ST115_0148:1:4:8937:164749#0    1024    chr3    58125506    60  100M    *   0   0   CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA    4734;<>=?@>@BB@DBCCCBAD@DBBB?@>>>ABBB?A>B??EBCB?B?@9@CCBC@@CC@AC@ACCC@A@@E@=@BCBABB@A?A@@?A@>?A@<>=9    BD:Z:JJNNNNPNOKLKJKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJKMMMNJMNNIMMMOOJOMIML   MD:Z:100    RG:Z:0.1    BI:Z:NNPQQQRQQOPOOPQPQNGGGNOLMMGPOPOJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONORRRORRRNPQQPPLOPMQP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:62:7763:195878#0   1024    chr3    58125506    60  100M    *   0   0   CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA    4724;<>=?@?>AA?DBBABBAD@DBBB>@>>=@BBB?@?B??DBBB?B??7@BBAC?@BB?AB?@CBB?AA?C@?@ABA@DB?@>@@?>A@>@@@:=;5    BD:Z:IIMMNMQMNLLKJKLJLI@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKJAJJLIJAKIJKIJAAKMLOMNMLMJJLLMNILMOJNLNONKNMIML   MD:Z:100    RG:Z:0  BI:Z:MMPPPQSPPOOONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOQOPQOOPQQQNQQRNPRQPOMNPLPO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:23:14784:196081#0  16  chr3    58125506    60  100M    *   0   0   CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTCTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA    :@:?B@?C@C@BBAABCCCBA@A@C@B?C???@BCBE@?BD9@BBA?BE?7@CCA?E@A@E@CD@CCCE@C?AC?@A?A<@AD?=A@C>?@>>B<<6533    BD:Z:OLKJMNNLLLKKMNNLBBBKNHIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHLJNHJIHJAAIKJMMMKLLJKKLMLHLKKHLMKLNJNQMNLJJ   MD:Z:59T40  RG:Z:0.1    BI:Z:QPONNOPPOPPPQRRQIIIOQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMPOQMONMOHHNPNNPPONPNOONPPLPPPMQONPOMQRPQPNN   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:26:20630:27949#0   1040    chr3    58125506    60  100M    *   0   0   CTGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATA    7?8=@?>BACABC@?BBBB7@@@@BBA?B>>=;@9BB@?BD?@BBB>BD?7@BCA>D@BBD@BA@BBBD@C??B@@@@A>?BD@>C@D@AC??B=93624    BD:Z:NKJIMNNLLKKKMMNKAAAJMIIKAJLLLJJLMKAIILLMMNJAJLLLLLJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKHLLJMMINPMNLII   MD:Z:100    RG:Z:0  BI:Z:PPONNOPOPPPOQQQQHHHOQNNPGOPPPJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLRROPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:2:18389:84462#0    0   chr3    58125507    60  100M    *   0   0   TGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATAT    43469<=<@A>BB@CBCCCCAD@EBBB?@>>>ABBB?@>B??DBBB>B??8@CCAC@@CC@AC@@CCC@AA@E@?ABEBAEC@@?A@A>B??=BA>@<:;    BD:Z:JJNKNQOOLLKKKMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJMMMNJMNNIMLMONKONIMLL   MD:Z:100    RG:Z:0.1    BI:Z:NNQORRRQOPOOPQPQOGGGNOLLNGOPPNJJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQRRORRRNPQQPQMPNMQPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:67:20358:45569#0   16  chr3    58125507    60  100M    *   0   0   TGAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATAT    ?;>???C?E?CBAAB@CCCAAAACDB=B????BBCD@?BD@@ACB?BE?6@BB@@D@BBD@CD@BBBDAC??C@@A@A>?@D@>B@D@AC@?C?<86264    BD:Z:LKJLNMMLLLKMNNLBBBKNIIJ@JKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLKLMJMPMQLLJJ   MD:Z:100    RG:Z:0.1    BI:Z:PONPNOOOPPPQRRQIIIPQMNOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOPOPNOOOONPPLOPPMQONPOMPROSPPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:24:19376:128752#0  0   chr3    58125508    60  100M    *   0   0   GAAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATG    45649;<?>@B@?CABCCBAD@EBCB?@>>>>>CCA@?BA@DCAB>B??7@ACBC@ACC?AB?ABCC?A@?D@>@BE@>EC@A<BAA<A%%%%%%%%%%%    BD:Z:JJKKQPPLMKKLMKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLMMNJMNNIMLLONJPNJMLLN   MD:Z:100    RG:Z:0.1    BI:Z:NNOPSRROPOOPQPQOHGGNOLLMHOOQNIJPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQRORRRNPQQPQNQOKQPPQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:26:19751:62521#0   0   chr3    58125509    60  100M    *   0   0   AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA    46447>?@<CB?EBCBCBAD@DBBB>@>>>@BCC?A?B@@DBCB>B??8ACC@C?ACC@AC?ACCCAA@?D?>A@DBAFBAA@?AA>A?>9B@??>;;;;    BD:Z:JJKNPQMMLKLNKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLMNJMNNIMLLNNJOOJNLLNN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSRPPOOPQPQOHHGNOLLMGPOPOIIPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQORRRNPQQPQNRPLOPPQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:44:13221:170521#0  1024    chr3    58125509    60  100M    *   0   0   AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA    36349:=<6@A?CBCBBC@D@DCCB??>>?;BCC@@?BA@ECCA?B??8>CC@CAACC?ADAACCC???@D?>A@EBBFB@A@AAA>@?>>@?>C@;<<<    BD:Z:JJKNPQMMLKLNKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLMNJMNNIMLLNNJOOJNLLNN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSRPPOOPQPQOHHGNOLLMGPOPOIIPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQORRRNPQQPQNRPLOPPQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:65:10341:97177#0   1024    chr3    58125509    60  100M    *   0   0   AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA    36459;>=:BBACBCBBCAE@DBBB>@>>>ABCCA@?B?@DCBB?B??7@CBBD@@CCA@C@@CCC@A@@EA>@BCB?DB@A@A@@?A??4B;@B?9<:<    BD:Z:JJKNPQMMLKLNKLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLMNJMNNIMLLNNJOOJNLLNN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSRPPOOPQPQOHHGNOLLMGPOPOIIPPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQORRRNPQQPQNRPLOPPQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:45:5212:195541#0   16  chr3    58125509    60  100M    *   0   0   AAGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGA    =>;9A@CABB@?CBBBB@?=?BBB?B>A>@CBBE@?BD??BBB>BD?7@BB@@D@BBD@BD@BBBDAC??C@@A@@>?BD@>C@D@AC@@D@?=>96854    BD:Z:ILLLKLLLLMNOLAAAJMIIKAJLLLJJLMKAIILLMMNKAJKLMMMJJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKJLLHLNJQOPMKII   MD:Z:100    RG:Z:0  BI:Z:NPOPLNNPPRQQQHHHOQNNPHOQPQJJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMRRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:65:19328:2272#0    0   chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    44429=<<BA?DACCBBAE@EBC?>A>>>ABCC?@>B?@CCAB?B??8@BCAD@ACC@AC@@CCC@A?@E@?@BCBBFC?A@B@@>A?>8AB@C%%%%%%    BD:Z:JJNMQNNLLLNLLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNIMLLNMJONKNMLNNM   MD:Z:100    RG:Z:0.1    BI:Z:NNQQSPQOOPQPQOHHHNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNPQQPQNRQMPNPQQP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:66:3484:54472#0    1024    chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTCTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    44439=<:AA?CAC@CBAD@DBBB>@>>>@BBB?@>B?@E?CB?B??7ACCAA@@CC@AC@ACCC@A??E@>A@C??DB@A?@@@>AA>A?B@EB;>:<<    BD:Z:JJNMQNNLLLNLLJJJIJKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNIMLLNMJONKNMLNNM   MD:Z:14T85  RG:Z:0.1    BI:Z:NNQQSPQOOPQPQOOONNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNPQQPQNRQMPNPQQP   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:7:12445:175053#0   1024    chr3    58125510    60  100M    *   0   0   TGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    43429>?@BB?EBCCBBAD@DBBB?@>>>ABBB?@>B??EBCB>B??7@CCAD?ACC@@C@@CCC@A?@E??@@CBAFC@A?B@@>A@>?@A@DB=>:<=    BD:Z:JJJMQNNLLLNLLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNIMLLNMJONKNMLNNM   MD:Z:0A99   RG:Z:0.1    BI:Z:NNNQSPQOOPQPQOHHHNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNPQQPQNRQMPNPQQP   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:7:1540:124569#0    1024    chr3    58125510    60  100M    *   0   0   TGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGCAGGGGACATATGAT    3456998:3<;B@@@BB?D@>??<<?</:8>>@<=?AA>ACCB>?=;4=AA7@AAB9????A=CC>@%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJJMQNNLLLNLLJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLNJMNNINNONJKMNKNMLNNM   MD:Z:0A84G3T10  RG:Z:0.1    BI:Z:NNNQSPQOOPQPQOHHHNOLLMGOPPNJIOPOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNRRRNRQRPKKQQMPNPQQP   NM:i:3  AS:i:89 XS:i:0
HWI-ST142_0240:1:2:19963:130770#0   1024    chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    44439=<9@@?B@AABB@D@DBBB?@>>>@BBB@??B??DBBB>B@?8@BB@B?@BB?@B@?BAB@A??D??@@B@@DB?;????>@@>8@@>B@<<::8    BD:Z:IINLQNOLKLMKMJ@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IIMIJAJIJKIJAAKMLOMNMLMJJLLMNILMNIMKMNMJOOKOMLNML   MD:Z:100    RG:Z:0  BI:Z:MMQPRQQONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPONPQQQNQQRNPQPPPORQLPMPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:14161:187574#0  1024    chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    44429==>@A?DBBBBBAD@DBBB?@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@CBB@A@?DA>@@BB@DB?@>@@?>A@><?A@C@<<8;8    BD:Z:IINLQNOLKLMKMJ@@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IIMIJAJIJKIJAAKMLOMNMLMJJLLMNILMNIMKMNMJOOKOMLNML   MD:Z:100    RG:Z:0  BI:Z:MMQPRQQONPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPONPQQQNQQRNPQPPPORQLPMPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:41:13983:94410#0   16  chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    ?;9?@CACCA@CCCCCA@A@CCC?DAAA@CCCEA?CE@@CCC?CE@8ACCA@D@BBD@CD@BBBEAC??C@@A@@>?BD@>C@D@AC@@EA?>@:;8564    BD:Z:LMLKLLMLNONLBBBKNIJKAJKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLILNKONOPLNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PPPOLNOPQRRQIIIPRNOOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPNOOOOOPPLOOOLQONPOMPQNQQQSPQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:47:12483:53170#0   1040    chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    ?;9??CABCAABCCCCA@A@CCC?CAAABCCCEA?BE?@CCB?CE@8ACCA@D@BCD@CE@BBCDAC??C@@@@@>?BE@>C@D@AC@@DA?>@:;8564    BD:Z:LMLKLLMLNONLBBBKNIJKAJKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLILNKONOPLNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PPPOLNOPQRRQIIIPRNOOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPNOOOOOPPLOOOLQONPOMPQNQQQSPQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:4:16748:194148#0   1040    chr3    58125510    60  100M    *   0   0   AGTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    =;:?@C@CCA?CCCCBA@A@@DC@EA?>ACCCEA?CE@@CCB?CE@8ABC@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA?>@:;8564    BD:Z:LMLKLLMLNONLBBBKNIJKAJKKKIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLILNKONOPLNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PPPOLNOPQRRQIIIPRNOOHOPPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPNOOOOOPPLOOOLQONPOMPQNQQQSPQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:43:2720:132802#0   1040    chr3    58125510    60  100M    *   0   0   ACTAGAGAATCATTTTTCATAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGAT    5/7==AABB@;?AAAB@??(ABB?C@A>BCCBDA>BD@?BBB?BD?7@BB@@D@BBD@AC@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA?>@:;8564    BD:Z:NMLJKLLLNNOLBAAJMNLJAJLLLJJLMKAIILLMMNKAJLKMMMKJ@IJMHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKILLHKNJOOOQKMII   MD:Z:1G17C80    RG:Z:0  BI:Z:QPPNLNOORRQQHHHOQQQQHOQQPKJOPPGNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPRQRPPMM   NM:i:2  AS:i:93 XS:i:19
HWI-ST115_0148:1:4:18448:107363#0   0   chr3    58125511    60  100M    *   0   0   GTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATG    44249;?AA?EBCCCB?D@EBBB>@>>>ABBB@@>B??EBBB>B??7@BBBC@ACC@AC@@CCC@AA@E@>A@C@AEC@A?A@@?A@>9@B@CB>A=<=;    BD:Z:JJMNNOMLMNLMJ@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMJMNNIMLLNMIONJOMMNNMN   MD:Z:100    RG:Z:0.1    BI:Z:NNQQQQPOPQPQOHHHOOLLMGOOQNIJOOOHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQRRNPQQPQNRQNQONQQPQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:3:19432:186788#0   1024    chr3    58125511    60  100M    *   0   0   GTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATATGATG    4324=:.=7:@>><?BAD@DBAA>@>>>ABBB?@?A@?DAB@?B??7?BB?AA?@>@@:>@:@B=@9=B??A@C>>CA@@=@@?>@?<#;>>B9>>6148    BD:Z:IILMOONKLNKMJA@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILIJAKHJKIJAAKMLOMNMLMJJLLMNILMNIMKLNKKLOKONMNMLN   MD:Z:88T11  RG:Z:0  BI:Z:MMPPQQQNPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNOOQQQNQQRNPQPPKKRRMPONPPPP   NM:i:1  AS:i:95 XS:i:20
HWI-ST142_0240:1:65:19856:17943#0   1024    chr3    58125511    60  100M    *   0   0   GTAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTTAGCAGGTCTCCATGTAGTGGAGGGGACATATGATG    43249<4A>>B@AAAB?D?DAAA>@>=>A;AAA>????DACA;A@?7?@@@>@ABB?@B?@BBAB?@>D@>@@B@>DA@@9?A?6>;=&6>>0?<>;;88    BD:Z:IILMOONKLNKMJA@@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILIJAKHJKIJAAAJMOMNMLMJJLLMNILMNIMKLNKKLOKONMNMLN   MD:Z:64G23T11   RG:Z:0  BI:Z:MMPPQQQNPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGGPPQOPOOPNOOQQQNQQRNPQPPKKRRMPONPPPP   NM:i:2  AS:i:90 XS:i:20
HWI-ST115_0148:1:24:11998:133823#0  0   chr3    58125512    60  100M    *   0   0   TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA    3336:>AA@DBCCCCAD@DCBB<@>>>ABBB?@>B??DCCB<BA?7@BCAB@ACC@AC?ACBCAA??E@?A@B@?EC@A?@AA>A??9@B?DB>A?>;=;    BD:Z:JJNKONMMOLMK@@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMIMNNIMLLNMINNJNNMONMNN   MD:Z:100    RG:Z:0.1    BI:Z:NNQORPPPQPQOHHHOPLLMGOOPOIIPONHONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQRNPQQPQNRQNRPOOQPQQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:27:1639:51624#0    1024    chr3    58125512    60  100M    *   0   0   TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA    32269=?@>B=B??BAD@DBAB>@>;;?ABB@=?B@>CBBB>B??7@BBAC?@BB?@B?@BBC@A@?D@>@@B@>DB?@?@@?>A@>;@,8453=>=988    BD:Z:IIMKPNMLNLMJAA@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHJAKIIKIJAAKMLOMNMLMJJLLMNILMNIMKLMMJNNKONNOMLNM   MD:Z:100    RG:Z:0  BI:Z:MMPOQQPPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNPPQQNQQRNPQPPPNQRNQOPNPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:63:2606:53050#0    1024    chr3    58125512    60  100M    *   0   0   TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA    203;;5==<@AAAAAAD@DBBB>@>>>ABBB?@>B??DBAA>B??7@BBAB@?BB@?A@@BBC?A@>C?>@@B?>EB?@?@@A=A@?5>7:@=;===::7    BD:Z:IIMKPNMLNLMJAA@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHJAKIIKIJAAKMLOMNMLMJJLLMNILMNIMKLMMJNNKONNOMLNM   MD:Z:100    RG:Z:0  BI:Z:MMPOQQPPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNPPQQNQQRNPQPPPNQRNQOPNPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:65:11877:144770#0  1024    chr3    58125512    60  100M    *   0   0   TAGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGA    213::<>@>BABBBBAD@=B@B=?>>>A?BB>??B@?>@BB:B@?7@BB@@@?BB?@B@@BBBA@>>C@?ABB@@BA?@?=?A>A???@?8>:8:>:78:    BD:Z:IIMKPNMLNLMJAA@IJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHJAKIIKIJAAKMLOMNMLMJJLLMNILMNIMKLMMJNNKONNOMLNM   MD:Z:100    RG:Z:0  BI:Z:MMPOQQPPPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNPPQQNQQRNPQPPPNQRNQOPNPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:24:2929:82313#0    0   chr3    58125513    60  100M    *   0   0   AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    3445<>@@CBBBBBAD@DBBB>@>>>ABBB?@>B??EBBB>B??7ABBAC@@CC@@C@@CCC@A@@E@>A@CBADC@@?AA@?BA>A@B?DB?B@?>;<8    BD:Z:JJKLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:43:9326:76966#0    1024    chr3    58125513    60  100M    *   0   0   AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    2277;==8?@CCCC@CAECBB<A>>>>BCC?@>B??E@AB?BAA8@CCACAACC?AC@ACCC?A@@E@=A@B@?DB@A??@@?B==9:@-?1:?=?><%%    BD:Z:JJKLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:48:1537:53772#0    1024    chr3    58125513    60  100M    *   0   0   CGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    4*46:=>>@@BCCCAC?DCCA?@?>>ABCC?@?BA@ECCB>BAA7@CC@C?ACC@AC?ACCC@A?=D@>@@C@?E@@A??@@>AB,54,4A??B@?>:;;    BD:Z:JJNLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM   MD:Z:0A99   RG:Z:0.1    BI:Z:NNQPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:65:2864:31597#0    1024    chr3    58125513    60  100M    *   0   0   TGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCCCCATGTAGTGGAGGTGACATATGATGAC    3345;??>CABBBBAD@DBBB?A?>>ABCC?@?B?@DCCB?B??7@CCAC?@CCA@C@ACCC@B?>C??A@?>?BB@A??AA>A?>@@@??A;A%%%%%%    BD:Z:JJNLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMLIILLMILNNIMLLNMINMJNMNOOMNNM   MD:Z:0A70T28    RG:Z:0.1    BI:Z:NNQPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQOKKQQQNQQQNPQQPQNRQNRQPPOPQQP   NM:i:2  AS:i:94 XS:i:21
HWI-ST115_0148:1:8:6415:195009#0    1024    chr3    58125513    60  100M    *   0   0   AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    3356;??>DABBBBAD@DBCB?@>>>ABBB?@>B??EBCB?B??7ACCAC?@CC@AC@ACCC@AA?D?>A?C@?DBAA??AA>B@9@@A@EB?BA@>:<;    BD:Z:JJKLNNNOMMKA@@JKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILNNIMLLNMINMJNMNOOMNNM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPQPQQPQOHHHOPMLMGOOPNJIOPNGONQPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQNPQQPQNRQNRQPPOPQQP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:21:15989:132253#0  1024    chr3    58125513    60  100M    *   0   0   TGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    3456:>>>A@BBBBAD@DBBB?@>>>ABBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB@A@?E@>@@C@ADC?@>@=?>@@>@@<:6365=?;:79    BD:Z:IIMLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMLJNNJONNPNLNMM   MD:Z:0A99   RG:Z:0  BI:Z:MMPOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPPNQQNRPPPNPPPP   NM:i:1  AS:i:99 XS:i:21
HWI-ST142_0240:1:26:3977:39672#0    1024    chr3    58125513    60  100M    *   0   0   AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    3245:?>>B@BBBBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB@@@?E@>A@DBADC?@?@=?>@@>@?@@DB?B?>=:;6    BD:Z:IIKLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMLJNNJONNPNLNMM   MD:Z:100    RG:Z:0  BI:Z:MMOOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPPNQQNRPPPNPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:27:20026:127868#0  1024    chr3    58125513    60  100M    *   0   0   TGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATATGATGAC    3345=>?>BABBBBAD>DBBB>@>>>@BAB???B??CCBB>B??7@BBAC?@BB?@B?@@BB@A??D@>@BDB>BC?@?@;?>@?>&;91<*9==?;893    BD:Z:IIMLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMJKLNJONNPNLNMM   MD:Z:0A85T13    RG:Z:0  BI:Z:MMPOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPKKQQNRPPPNPPPP   NM:i:2  AS:i:94 XS:i:21
HWI-ST142_0240:1:62:8654:148039#0   1024    chr3    58125513    60  100M    *   0   0   AGAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAC    4356:>@?BABBBBAD@DBBB?@>>>@BBB?@?B??DBBB>B??7@BBAC?@BB?@B?@BBB@A@?E@>ABBBADC?@>@@?>@@>@@?@CB?B??<::6    BD:Z:IIKLOMNNLNJAAAIJGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHIAKIJJIJAAKMLOMNMLMJJLLMNILMNIMKLMLJNNJONNPNLNMM   MD:Z:100    RG:Z:0  BI:Z:MMOOQPRPPPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOQPQNQQRNPQPPPNQQNRPPPNPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:64:12047:152217#0  16  chr3    58125514    60  100M    *   0   0   GAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACG    ;><?AAABCCACA@A@CCB?DAAABCCCEA?CE?@CCB=CE?7ACCAADABCD@CD@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA@?B>AC?>=53+4    BD:Z:KKKKNOPMCCBKNIJKAKLLLIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMINLLNMPPONMJJ   MD:Z:100    RG:Z:0.1    BI:Z:NOOONPQQIIIPRNOPIPQPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPONQPLPOOLPNMOOMPQNQPPQPRRRQQNN   NM:i:0  AS:i:100    XS:i:20
HWI-ST115_0148:1:65:11545:192642#0  1040    chr3    58125514    60  100M    *   0   0   GAGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTCGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACG    =?;?@A?BCCCCA@A@CCC?CAAABB@CEA?CE@@CCC?BE@7ACCAADABBD@@7@BBBDAC?AC@@@@@>?BD@>C@D@AC@@DA@?B>AC?>=54+4    BD:Z:KKKKNOPMCCBKNIJKAKLLLIILMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHLMKJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMINLLNMPPONMJJ   MD:Z:55T44  RG:Z:0.1    BI:Z:NOOONPQQIIIPRNOPIPQPPKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMPQOOHHNPNOQPPOPOPONQPLPOOLPNMOOMPQNQPPQPRRRQQNN   NM:i:1  AS:i:95 XS:i:20
HWI-ST142_0240:1:47:16052:133779#0  1024    chr3    58125515    60  95M5S   *   0   0   AGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATAGATC    3287;<BAAABBAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?AA?D@?@@DBACC?A>@@?;A@>A?AADB>B?AA=;;;9    BD:Z:IIKJOPNNKBAAJKGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JIJKIIAAKMLOMNMLMJJLLMNILMNIMKLMLIMNJNMNPONMMKLM   MD:Z:95 RG:Z:0  BI:Z:MMONRRRPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPQMQQRNPQPPPNQQMQQRQPPMPOPP   NM:i:0  AS:i:95 XS:i:19
HWI-ST142_0240:1:5:17706:32015#0    0   chr3    58125515    60  100M    *   0   0   AGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGT    4756;=BAABBBAD@DBBB?@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@A?D@?@ACBAEC?A>@@?<@@>@@@@DB>B?@A=;;27    BD:Z:IIKJOPNNKBAAJKGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JIJKIIAAKMLOMNMLMJJLLMNILMNIMKLMLIMNJNMNPONOMMKL   MD:Z:100    RG:Z:0  BI:Z:MMONRRRPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPQMQQRNPQPPPNQQMQQRQPPNPPNO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:62:21194:135565#0  1024    chr3    58125515    60  100M    *   0   0   AGANTCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGT    23.%;;=>>>BBAD@DBBB>@>>>@BBB?@>B??DBBB?B??7@BBAC?@BB?@B?@BBB?AA?D@>@@BBAEC?A>@@?4@@><?>=>>>BAB?==3.7    BD:Z:IIKIMLNNKBAAJKGHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JIJKIIAAKMLOMNMLMJJLLMNILMNIMKLMLIMNJNMNPONOMMKL   MD:Z:3A96   RG:Z:0  BI:Z:MMOMOORPNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPQMQQRNPQPPPNQQMQQRQPPNPPNO   NM:i:1  AS:i:98 XS:i:19
HWI-ST142_0240:1:44:17812:198584#0  16  chr3    58125515    60  100M    *   0   0   AGAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGA    <9==?>BBBBAA@@?BBB>C@@@ACBBEA?CD?@BBB?BD?7@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>A@D@AC@@DA@?B>BC@@@:4+54    BD:Z:JJJLNPMCCBKNJIKAJLLLJJLMKAIILLMMNKAJLLMMMKJAJKNHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLINLLNLNPNQLKII   MD:Z:99T0   RG:Z:0  BI:Z:NONPNPPHIIOQNNPHOQQQKKPQPHNLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPRRRONMM   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:46:21053:166072#0  1040    chr3    58125515    60  100M    *   0   0   AGAATCATTTTTCACAAATAGGGGATTTGTATACAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGT    %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%>=@=>=A<8>87+02    BD:Z:JJJLNPMCCBKNJIKAJLLLJJLMKAIILLLLIKAJLLMMMKJAJKNHJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLINLLNLNPNQLKII   MD:Z:32G67  RG:Z:0  BI:Z:NONPNPPHIIOQNNPHOQQQKKPQPHNLOOPPMOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPRRRONMM   NM:i:1  AS:i:95 XS:i:20
HWI-ST115_0148:1:67:16722:167166#0  0   chr3    58125516    60  100M    *   0   0   GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGCGACATATGATGACATG    3467;B@BBBC?C@EBBA?@>?>/ACC@>>BA?CA>A>A@?7ACC@C@AC@A@<?ACCC@A??D?=@;C??<B@B@B@@=A?%%%%%%%%%%%%%%%%%%    BD:Z:JJKLQOOLBAAKKHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHMLLNNLNMIMLMOOOOOMIMN   MD:Z:83T13G2    RG:Z:0.1    BI:Z:NNOPSQROHHHOPMMNHOOPNIIOPNGOMPPQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPQPPORQNRQQRRPPOPMQQ   NM:i:2  AS:i:92 XS:i:19
HWI-ST142_0240:1:43:18744:44408#0   1024    chr3    58125516    60  100M    *   0   0   GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGAG    3568;@@AAABAD@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?A@@D@>@ABBAEC@@?@@?>4?>>@@>B?<BAAA?%%%%%    BD:Z:IIJKQNPKBBAJKHHH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHJKIJ@AKMLOMNMLMJJLLMNILMNIMKLMLIMMJNMMPONPNMKML   MD:Z:98T1   RG:Z:0  BI:Z:MMNPRRRNGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPNPQRNPQPPPNQQMQPRRQPPNPNPO   NM:i:1  AS:i:98 XS:i:19
HWI-ST115_0148:1:6:1840:111707#0    16  chr3    58125516    60  100M    *   0   0   GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG    ;>==>BC@CCA@A@CC=@EB?BBBDCD@?BD?AB?B?BE;7@BCBAEACCE@CBACCCEAC@ACAAAAA>?BEA?C@E>@D?@EA@@B?BE@AC>:,493    BD:Z:KKMNOMDCCLNIJKAKLLLJJLMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKLMLONNQPLIJJ   MD:Z:100    RG:Z:0.1    BI:Z:OOPQOOHIIPRNOPIPQQQKKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOPPMOOPLPNMONLPQNQPPQPQQQRSOMNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:46:18554:196838#0  1040    chr3    58125516    60  100M    *   0   0   GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG    6<=;=@BB@B@@@@BBB?B@?=@ABAC??BE??CBB>CD>7>CB@@D@BBD@CB@BBBDAC?AB@@@@A>?BD@>B@D@AC=@D:@?B>AD@AB=:+484    BD:Z:JJLMOMCCCKNJJKAJLLLJJLMKAIILLMMNKAJLLMMMKK@JKNIJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHNLLMLNNNPPKHII   MD:Z:100    RG:Z:0  BI:Z:ONPPNPGHIPQNNPHOQQQKKPQQGOLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPRRQNLMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:5:7801:61872#0 1040    chr3    58125516    60  100M    *   0   0   GAATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG    <==;=ABCAB@@A?BBB?C@@@@BBCCA?CE??CBB>CD?7@BB@@C@BBD@BC@BBBD@C?@C@@@@@>?BD@>C@D@AC@@D?@?B>BD@AB=9+484    BD:Z:JJLMOMCCCKNJJKAJLLLJJLMKAIILLMMNKAJLLMMMKK@JKNIJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHNLLMLNNNPPKHII   MD:Z:100    RG:Z:0  BI:Z:ONPPNPGHIPQNNPHOQQQKKPQQGOLOOPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPRRQNLMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:7:2991:103346#0    1040    chr3    58125516    60  100M    *   0   0   GAATCATTTTTCACAAATAGGGGATTTGTATGCAAACATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTG    399==??AAA=>>?::@8A4>>>BBBC@?BD?@BB@@?C>3>BBA@B?BBD@AD@AAB=>A<788=@<>2>>C?>@=B==B=?C??=?>@@===/;/372    BD:Z:JJLMOMCCCKNJJKAJLLLJJLMKAIILLMMNKAJINMMMKK@JKNIJ@HHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHNLLMLNNNPPKHII   MD:Z:36T63  RG:Z:0  BI:Z:ONPPNPGHIPQNNPHOQQQKKPQQGOLOOPOPOGMMPPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPRRQNLMM   NM:i:1  AS:i:95 XS:i:20
HWI-ST115_0148:1:5:3749:99449#0 0   chr3    58125517    60  100M    *   0   0   AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC    3656?>@ABB?C?DBBB?@>><BACC@?<;@?BC5B:A@>7@C;@CA@CC@@??>7?=?0==A>7A?A=<=>?;=?%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJLNOPMBBAKLHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLLLNMINMIMLLOONPONLMIN   MD:Z:100    RG:Z:0.1    BI:Z:NNPQRRPHHHOPMMNHPOPNIIOOOGNNPOQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPPQNRQNRQQRRQQPNOQMQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:65:19877:44416#0   1024    chr3    58125517    60  100M    *   0   0   AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAAGTGC    3656?>@AAA?C?DCBA=?>>>=BCC@??B@?CCCB?B@?7ACC@B@ACC@ABAA?BBAA??D>=A@A@?DB@A??A@?>?<??<@%%%%%%%%%%%%%%    BD:Z:JJLNOPMBBAKLHHIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLLLNMINMIMLLOONPOLKNIN   MD:Z:95C4   RG:Z:0.1    BI:Z:NNPQRRPHHHOPMMNHPOPNIIOOOGNNPOQQPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPPQNRQNRQQRRQQPMPQMQ   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:47:6693:50311#0    1024    chr3    58125517    60  100M    *   0   0   AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC    3545>>;>>@:C><@=A:==<<:<8>>>:8@=A@<=<?@?7?@@>%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:IIKMOPMBBBJKHIH@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIKIJA@KMLOMNMLMJJLLMNILMNIMKLMLIMMINMMOONPONKLIN   MD:Z:100    RG:Z:0  BI:Z:MMPPRRPGGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMQPRNPQPPPNQQMQPQRRQPPNNOMP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:24:11281:81065#0   16  chr3    58125517    60  100M    *   0   0   AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC    >=;=ABBCCA@AACCB?DA?ABCCCE@?BE?@CCC?BE@7ACCAAEACBE@BD@BBBEAC?AC@@@@@>?BD@>C@D@AC@@DA@?B>BDAAC?<04834    BD:Z:KMNNMCDCLOIJKAKLLLJJMMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKMLNNMPPOINJJ   MD:Z:100    RG:Z:0.1    BI:Z:OPQQNGHIPRNOPIPQQQLKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQPLPOOMPNMONLOQNQPPQPQQPRRQMPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:28:14521:122053#0  1040    chr3    58125517    60  100M    *   0   0   AATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGC    ;=;<@BCCCAAA@BCC?CB??BCDDE@?BD??CCB?BE99@CCABEA?DE@CCACCCEAC@@BAAAAA>?BEA<CAEAAAAACA@?B?BEAAD?<25733    BD:Z:KMNNMCDCLOIJKAKLLLJJMMKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKMLNNMPPOINJJ   MD:Z:100    RG:Z:0.1    BI:Z:OPQQNGHIPRNOPIPQQQLKOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQPLPOOMPNMONLOQNQPPQPQQPRRQMPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:8:10443:148224#0   0   chr3    58125518    60  100M    *   0   0   ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC    3539<>?@@?<=A=B?=?><=@AAA?><A@=??A>>?A?8/>@>AAA@@?@B@@BBB@>@>@?:?@B@>CB@@????>A?,6?9<DA?A%%%%%%%%%%%    BD:Z:IIMKQMDBBKKHII@JKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJIJAAJMLOMNMLMJJLLMNILMNIMKLMLIMMIMMMONNPOOLLINM   MD:Z:100    RG:Z:0  BI:Z:MMPPRPIGGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPQQNPQPPPNQQMQPQQRRQPPLOMPO   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:47:6712:171630#0   16  chr3    58125518    60  100M    *   0   0   ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC    <=;>A@/A??A@;BB?C<?=?BBBD??BD?@CBB??D?7@CCA@D@CCD@CEAACCDAD@AC;@B>A<ABEA?@AE=AD?AE@@?B?BE?BD@>3;:323    BD:Z:MNNLCCDLOJJKAKLLLJJMNKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLLNMMOOOLNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQQPFGHPRNOPIPQQQLLOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQLOPOLQNMONLOPNQPPQPQQPQRPOPONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:63:14295:160964#0  1040    chr3    58125518    60  100M    *   0   0   ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC    ;<;>ACCCBA<?;==?C>?<@BCBD@?BE?@CAB?BE?7=CCAAFACCFACD@CACDAC?ABAAA@A>A@D@?C;E?@DAAE@??B?AEAAD@=5;9213    BD:Z:MNNLCCDLOJJKAKLLLJJMNKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLLNMMOOOLNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQQPFGHPRNOPIPQQQLLOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQLOPOLQNMONLOPNQPPQPQQPQRPOPONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:7:6785:81212#0 1040    chr3    58125518    60  100M    *   0   0   ATCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCC    =<<>ACB?A?AACCB@CB?>ACCCD@?BD?@CCB?BE?7?CCA@EACCF@BD@CBBDAC?AC@@A@@;?BD@>CAD@AC@@D???B>BDABC@>39:324    BD:Z:MNNLCCDLOJJKAKLLLJJMNKAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLLNMMOOOLNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQQPFGHPRNOPIPQQQLLOQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQLOPOLQNMONLOPNQPPQPQQPQRPOPONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:41:14818:141318#0  0   chr3    58125519    60  100M    *   0   0   TCATTTTTCATAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT    3477=?@A:B@9A>@;?>>?1BCAAA>B@;3:5?.B@>5ACC@AAACCAAA@ACCC@A>?D@>A@C@?EBAA?@A@>A??<@==BB?B@@@@=;4?<18=    BD:Z:JJLMNDCBLLMLIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKNMINMIMLLNNNOOOMNINNL   MD:Z:10C89  RG:Z:0.1    BI:Z:NNPQQIIHOPQPNHPPQNIIOONGOMPPPPPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPNRQNRQQRRQRRPNOMQPP   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:61:16724:48061#0   1024    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGGCATATGATGACGTGCCT    4586;>@A@E@DBBB>@>>>ABBB?@>B??EBCB>B??7@BBAC?@BB?@C?ACCC@AA@E@?A@DBAECAA?A@@?AB>>@>?DB?B@A@@B@7>=9:;    BD:Z:JJLMNDCBLLIIIAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKNMIMNNMLLNNNOOOMNINNL   MD:Z:82A17  RG:Z:0.1    BI:Z:NNPQQIIHOPMMNHPPQNIIOONGOMPPPPPNHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPNPPQRQQRRQRRPNOMQPP   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:23:18737:20226#0   1024    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT    4586;>@A@D@DBBB?@>>>@BBB?@?B??DBBB=B??8@BBAC?@BB?@B?@BBB??@?D@?@ACB@BC@@?@@@>A??3@@@DB?B@?A=@=5<<77;    BD:Z:IIKMNDDBKLHIIAJKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHJAAKLLOMNMLMJJLLMNILMNIMKLMLIMMIMLMONMPOOMMINML   MD:Z:100    RG:Z:0  BI:Z:MMPPPIIGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPRMPQPPPNQQMQPQQQRRQPNMMPOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:25:11308:176882#0  1024    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT    3377<>?@>=;CB;>9?>><=@AA?;99<>DBB@;?@=8@B?@A@@B@@@;A@BBBAA=>D@8A?A@>CAA@>?@A>A@?0A@9C?=??=8?@>4<7859    BD:Z:IIKMNDDBKLHIIAJKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHJAAKLLOMNMLMJJLLMNILMNIMKLMLIMMIMLMONMPOOMMINML   MD:Z:100    RG:Z:0  BI:Z:MMPPPIIGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPRMPQPPPNQQMQPQQQRRQPNMMPOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:62:8076:104111#0   1024    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT    4586;>@A?D@DBBB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@?@ACB@DC@A?A@@>A@>=?A@DB?B@@B?A>6=;789    BD:Z:IIKMNDDBKLHIIAJKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHJAAKLLOMNMLMJJLLMNILMNIMKLMLIMMIMLMONMPOOMMINML   MD:Z:100    RG:Z:0  BI:Z:MMPPPIIGOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPRMPQPPPNQQMQPQQQRRQPNMMPOP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:3:17353:40399#0    16  chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA    ;;<?@CCA@A?CCB?A?BBBBBCD=?BD?@>BB;BD?7@@CA?D@9CEBCE?CCCEBD@?DAAA?A>?BEA?D@E?AD?AEAA@A?BEABC??4<?4253    BD:Z:NNLBCCMOJKKAKLLLJJMNLAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKNMLONNLQNMJJ   MD:Z:99T0   RG:Z:0.1    BI:Z:QQPHFGORNOPIPQQQLLPQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMOOPLPOMONLOPMQPPQPQQPQQPNROONN   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:4:12205:180848#0   1040    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT    <<=>BBBA@@@CCC?D?AABBCDE@?CE?ACCC?BE@7ACCAAEACCD@@D@BBBDAC@?B@@@@@>?BE@?@@E@@C@@D@@?B>BDABD@?5<=3244    BD:Z:NNLBCCMOJKKAKLLLJJMNLAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKNMLONNLQNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QQPHFGORNOPIPQQQLLPQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMOOPLPOMONLOPMQPPQPQQPQQPNROONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:64:10493:106639#0  1040    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA    ;;<?9:?=;8=B?C?EA??@BDCF@?BD?@BCB?BE@7@CCA?EBCCE@CDACCCCBD@@D?@>@A>?AD@?DAE?@A?@E>??B?BE?BE@?6>?4133    BD:Z:NNLBCCMOJKKAKLLLJJMNLAIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKNMLONNLQNMJJ   MD:Z:99T0   RG:Z:0.1    BI:Z:QQPHFGORNOPIPQQQLLPQPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMOOPLPOMONLOPMQPPQPQQPQQPNROONN   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:25:4650:187088#0   1040    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTGTGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCT    8:<=@ABA@@?BCB>C@@@ABBADADAD@@CBB?BD?8@BBA@D@BBD@BD@BBBDAC??C@@A@@>?AD@>C@D@AC@@DA@?B>BDABD@?5<=3244    BD:Z:MNKABCLOKJLBKLLLJJLMKAIIHIHMNKAJLLMMMKKAJKMIKAIHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKMKMMLNMNKQMMII   MD:Z:26A73  RG:Z:0  BI:Z:PPPGEGNQOOPHOQQQKKPQQHOMLMLOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPOPNQOOMM   NM:i:1  AS:i:95 XS:i:0
HWI-ST142_0240:1:44:10947:22007#0   1040    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA    8:==@ABA?@?BCB?C@@@ABBBDA?CE@@CBB?BD?8@BB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@D@AC@@DA??B>BDABD@?5<=3124    BD:Z:MNKABCLOKJLBKLLLJJLMKAIILLMMNKAJLLMMMKKAJKMIKAIHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKMKMMLNMNKQMMII   MD:Z:99T0   RG:Z:0  BI:Z:PPPGEGNQOOPHOQQQKKPQQHOMOPPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPOPNQOOMM   NM:i:1  AS:i:99 XS:i:19
HWI-ST142_0240:1:68:15360:190460#0  1040    chr3    58125519    60  100M    *   0   0   TCATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCA    9:<=@AB@?@?BCB?C@@@ABBBDA>AC@@CBB?BD?8@BBA@D?CBD@CD@BBBDAB??C@@A@@>?BD@>C@D@AC@@D<@?B>BDABD@?5<=3234    BD:Z:MNKABCLOKJLBKLLLJJLMKAIILLMMNKAJLLMMMKKAJKMIKAIHJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKMKMMLNMNKQMMII   MD:Z:99T0   RG:Z:0  BI:Z:PPPGEGNQOOPHOQQQKKPQQHOMOPPOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPOPNQOOMM   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:23:6094:167071#0   0   chr3    58125520    60  100M    *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA    3857=?A>CACBBB>>=>>=BCC@A?B??EACB>B??8@CCAC@@CC?@CA@CCCAA?=D?>@@D@AEB?A>?A@>A?>@@A@DB?BAABAAA5@>:9>%    BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOONNNJNNLL   MD:Z:100    RG:Z:0.1    BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQOPKQPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:26:13769:48947#0   1024    chr3    58125520    60  93M7S   *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACAGATCGG    3866;?A>D@DBCB>?==>@BCC?@>B??ECCB?B??7@BB@C?@CC?AC?ACCC@AA?E@=A@DBABBAA??@@>A?:A@A@DB?B@ABA@@C??:;29    BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOONKPLMNLM   MD:Z:93 RG:Z:0.1    BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQMPMPQOO   NM:i:0  AS:i:93 XS:i:19
HWI-ST115_0148:1:46:3303:72160#0    1024    chr3    58125520    60  92M8S   *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAGATCGGAA    3856;@@@C@DBBB>@>>>@BBC?A?B??EBBB>B??7@BA@C@ACC?@C?@CCC@AA@E@>ABDBADC@A?AAA?A??4@=@DB?B@AB@A??=>496;    BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOOMMNOLMLK   MD:Z:92 RG:Z:0.1    BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQOOOOOPO   NM:i:0  AS:i:92 XS:i:19
HWI-ST115_0148:1:4:18275:87094#0    1024    chr3    58125520    60  100M    *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA    4866;>@@C@DBCB>A>>>ABBB?@>B??DBBB>B??7@BBAC?@BB?@B@@CCC@A@@EA>@BCBAEC@A?AA@>@@??A@@CB=BABA@B?5@?;9>8    BD:Z:JJMKDDCLMIIJAKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMINMIMLLNNMOONNNJNNLL   MD:Z:100    RG:Z:0.1    BI:Z:NNQOJIIOPMMNHPPQOIIOONGNNPOQPONHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMRQNRQQRRQRRQOPKQPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:43:2951:146335#0   1024    chr3    58125520    60  98M2S   *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCAC    4756;>>?C?CBBB?>===?8AA?==A@>CBBB?B??8@BB@A?@BB??B?@BBB?B@?D@>@@C@?D>@@?@@@>A?>5)::>?>A@A@?A@7?%%%%%    BD:Z:IIMJEDDKLIIIAKKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHIAAKMKOMNMLMJJLLMNILMNIMKLMLIMMIMLLONMOOOMNJNMLH   MD:Z:98 RG:Z:0  BI:Z:MMPNIIIOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQNOQPPPNQQMQPQQQQRRQNOKPOPL   NM:i:0  AS:i:98 XS:i:19
HWI-ST142_0240:1:8:13137:17336#0    1024    chr3    58125520    60  92M8S   *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGAGATCGGAA    4756;>@?C?BBBB>@>>>?BBB?@?B??DBBB?B??7@BB@C??BB??B??BBB?B@>D?>?>B@@CA?A?@@@>A?>@8@=A@=??A5@B?A9<36:%    BD:Z:IIMJEDDKLIIIAKKLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHIAAKMKOMNMLMJJLLMNILMNIMKLMLIMMIMLLONMOONMNNKLKJ   MD:Z:92 RG:Z:0  BI:Z:MMPNIIIOPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQNOQPPPNQQMQPQQQQRRPOPNNMPN   NM:i:0  AS:i:92 XS:i:19
HWI-ST115_0148:1:22:12391:16829#0   16  chr3    58125520    60  100M    *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA    ;=<?@AAAAACCB?BB?BBCCCFB@BD@@B@B?BE@7@CDAAEACCEA=EACCCEAC@AB?@AAA>@BEA?DAE?@B?@DA@?B?BE?BDA?7?B:3423    BD:Z:NLBBCLPJKLAKLLLJJMNLBIHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMMLNNMKQQNNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPHHFNQNOPIPQQQLLPRPHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPOOMPNNONLOPMPPPQPQQPQQONQQOQNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:1:18497:115417#0   1040    chr3    58125520    60  100M    *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA    9<;=@AA???BBC>C@@?ABBBD@?BE@@BCB?CD?7?BB@@D@BBD@BD@BBBDAC??C@@A@@>?BD@?C@D@AC@@DA@?B>BDABDA?6>@82424    BD:Z:NKAABLOKKLBKMLLJJLMKAIILLMMNKAJLLMMMKKAJKNHKAIIJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLKMMKNMLKPQMMII   MD:Z:100    RG:Z:0  BI:Z:PPGGENPNOQHOQQQKKPQQHOMPOQOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONNQQOPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:42:16985:26745#0   1040    chr3    58125520    60  100M    *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA    8===@A@@@?BBC?C@@>ABBBD@?AD@@BCB?@D?7ABB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>B@D@@C@@C@@?B>BDABDA?6>@82424    BD:Z:NKAABLOKKLBKMLLJJLMKAIILLMMNKAJLLMMMKKAJKNHKAIIJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLKMMKNMLKPQMMII   MD:Z:100    RG:Z:0  BI:Z:PPGGENPNOQHOQQQKKPQQHOMPOQOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONNQQOPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:7:15321:9803#0 1040    chr3    58125520    60  100M    *   0   0   CATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTA    :=<=@A@?@?BBC?C@@@ABBBD@?BE?@CBB>CD?8ABB@@D@BBD@BD@BBBDAC??C@@@@@>?BD@>C@A@AC@@DA@?B>BDABDA?6>@82423    BD:Z:NKAABLOKKLBKMLLJJLMKAIILLMMNKAJLLMMMKKAJKNHKAIIJHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLKMMKNMLKPQMMII   MD:Z:100    RG:Z:0  BI:Z:PPGGENPNOQHOQQQKKPQQHOMPOQOPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONNQQOPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:22:19130:140774#0  0   chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    4666;>?D?DBBB>@>>>@BBBA@>B??EBAB?B??8@BBAC?@CC?AC?ABCC@AA@EA>ABD@ADC@A?@AA?@?>>BA@DB?BAA?AA@7@@=;<:<    BD:Z:JJKADDMMJIJBKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLINMIMLLNNMNONMOJONLLL   MD:Z:100    RG:Z:0.1    BI:Z:NNOHJIPPMMNHPPQOJIOONGNMQOPQOMHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQQNRQQRRQRRQPQLOPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:8:14113:59358#0    1024    chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    4666;>?C?DBBB>@>>>@BBB@@?B@?DBBB>B??7@BCAC?@BA?@A?ACCC@AA>E@=A@C@ADB@A?@AA=A>>?@B@DB?B@AB@A=7AA7:<8;    BD:Z:JJKADDMMJIJBKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLINMIMLLNNMNONMOJONLLL   MD:Z:100    RG:Z:0.1    BI:Z:NNOHJIPPMMNHPPQOJIOONGNMQOPQOMHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQQNRQQRRQRRQPQLOPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:8:9706:101181#0    1024    chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    3568=??D@DCCA?A>==@ACC???A??EC>B>BA?7@CCAC?@CC?@C?ADCC@A=?D?>A@C@?CB@A??@A=@=<;@A@DB?B@A229,2=859<8;    BD:Z:JJKADDMMJIJBKKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLINMIMLLNNMNONMOJONLLL   MD:Z:100    RG:Z:0.1    BI:Z:NNOHJIPPMMNHPPQOJIOONGNMQOPQOMHPPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQQNRQQRRQRRQPQLOPPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:13388:89156#0   1024    chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    4666;>?C?DBBB>@>>>@BBB??>A??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@BCA@EB?A?A@@>@@>@?<@DB?B?AB?@@7@>=9<9=    BD:Z:IIJAEDMLIJIAKLLLIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@AKMLNMNMLMJJLLMNILMNIMKLMLIMMIMLLNNMONOMNKOMLLL   MD:Z:100    RG:Z:0  BI:Z:MMNGIIQPLLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMPPPPPNQQMQPQQQQQRROOMNOPPP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:27:5834:78263#0    16  chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    =>>?AA@AAC@B?B????BCCE@?BD?ACBB?BD?7@CCAAE@BBEACE@CCBDAC??C@@A@?>ABE@?C@E@AC@@E@A?A?ADABDA@6?B;74264    BD:Z:LBBBLOKKLBKLLLJJMNLBJHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLLNMMJPQQNLJJ   MD:Z:100    RG:Z:0.1    BI:Z:PHHHMPMOPIPQQQLLPRQHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPOLQNMPNLOPMPOPQPQQPQQOMQPQQPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:41:2831:14874#0    1040    chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    <>;?AAA@@CCC?C?A?@BACEA?CD?ACC@:BE?7ACCA@EABCEABEABBCDAC?AC?@A@A>?BE@?C@D@AC?@E>@?A>AEAADA@6?B;74263    BD:Z:LBBBLOKKLBKLLLJJMNLBJHLKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLLNMMJPQQNLJJ   MD:Z:100    RG:Z:0.1    BI:Z:PHHHMPMOPIPQQQLLPRQHNMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPOLQNMPNLOPMPOPQPQQPQQOMQPQQPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:25:19258:173665#0  1040    chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGTCTAT    7;6=?@??@?BB?C@>?=B@BC@>BD?AAA@?AC>7@BBA@D@AAB<@D?BBBD@B>AB>?A??>??D@=B@A;@C;<B???A>AD?BC?<5=@;<4243    BD:Z:KAAAKOKKMBKMMLJJLMKAIILLMMNKAJLLMMMKKAJKNIJAIIKHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJMMKMMLILPOMLII   MD:Z:95C4   RG:Z:0  BI:Z:PGGGLPMNQIOQQQKKPQQHOMPPPPPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLNRQPOMM   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:5:16655:34005#0    1040    chr3    58125521    60  100M    *   0   0   ATTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTAT    ;<:=@?@A?BB;?C@>>@A@BD@>BD??BB@??D>8@BB@?D@BBD@BD@BBBD@C>?C@@@@A>@BD??@@D@@A@@D??=B>BB@BC?>7?B;84254    BD:Z:KAAAKOKKMBKMMLJJLMKAIILLMMNKAJLLMMMKKAJKNIJAIIKHHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJMMKMMLIPPQMLII   MD:Z:100    RG:Z:0  BI:Z:PGGGLPMNQIOQQQKKPQQHOMPPPPPOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLQQQPOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:23:6146:104545#0   0   chr3    58125522    60  100M    *   0   0   TTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCGGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATC    3666==B@DBBB>@>>>ABCB?A?B?@ECCB?B??7@CCAC?@BB@@C?ACDC?AA>7?>A@D@?DBAA?@BA>B?>>@A>DB?B4<<:7=6@%%%%%%%    BD:Z:JJAADNNJJJBLKMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNKLILMJJLLLMILMMHLKKMLHNMIMLLNNMNNNMNKOOLLLN   MD:Z:57A42  RG:Z:0.1    BI:Z:NNHHJPQMMNHPPQOJJOONGNMPPPPPMGPPPQQNQNHOOPNNHONNONNHHOQPRNOJPQONOQQQNQQQMOPPOPMQPNRQQRRQRRQPRMPNPPPQ   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:26:17568:111462#0  16  chr3    58125522    60  100M    *   0   0   TTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATC    =<==>>@?BBB?D@@@@BB?D@?AE?@CCC?BD@7@BC@@D@BBD=BD@BBBDAC??B@@@@@>?BD@>B@D@AB@@DA@?A?BDABDA@7?C=:92644    BD:Z:AAAJNKKMCKMMMJJLMKAIILLMMNKAJLLMMMKKAJKNIK@IIKIHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLMKMLLINPPQLLII   MD:Z:100    RG:Z:0  BI:Z:GGGNNMMPIPQQQKKPQQHOMPPQOQOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOQQROPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:48:19526:151624#0  1040    chr3    58125522    60  100M    *   0   0   TTTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATC    ;:<=??@@?B>?B>>??A<8C??BC>@3:=>>D?7?BBA@D?<BB@CB@BBCC@A<?>;-A>@>@>@??A;A<<8=>C=>?><AB?<A=:6>B<;:4543    BD:Z:AAAJNKKMCKMMMJJLMKAIILLMMNKAJLLMMMKKAJKNIK@IIKIHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLMKMLLINPPQLLII   MD:Z:100    RG:Z:0  BI:Z:GGGNNMMPIPQQQKKPQQHOMPPQOQOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOQQROPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:23:21094:189737#0  0   chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCT    4668:@>CBBB>@>>>>ABB?A>A@?ECCB?B??7@BB@C@@CCAAC@@BCCAA@>B?>A@C?>CB@A?@@@>@@>:B@3BB=@BB@<A16??<2@::73    BD:Z:JJAANOKJKBLLMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMMIMLLNNMNNMMNJPOMLLNK   MD:Z:99C0   RG:Z:0.1    BI:Z:NNHHQQNMNHPPQOJJPONGNMPOQPONGOPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMRQQRRQRRQPRNQONPPQO   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:24:7477:78408#0    1024    chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC    4666:B>CABB>@>>>@BBB?@?B??DBBB>B??7@BBAC?ABC?AB?@CCB@@A@EA?ABCBAEC@A?AA@>A?>AAA@EB?BABB@A?7A@??A;<;8    BD:Z:JJAANOKJKBLLMMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMMIMLLNNMNNMMNJPOMLLNM   MD:Z:100    RG:Z:0.1    BI:Z:NNHHQQNMNHPPQOJJPONGNMPOQPONGOPPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMRQQRRQRRQPRNQONPPQO   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:43:9326:167668#0   1024    chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATTC    4666:@>CABB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACB@DB@@>A@@?A@=@?A@/?=??AB@A@7?@>=?97&7    BD:Z:IIAANNKJJBKLMMIIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JMLOMMMLMJJLLMNILMNIMKLMLIMMIMLLNMLONNLNKPOMLLJJ   MD:Z:98C1   RG:Z:0  BI:Z:MMGGQRNLNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPPOPNQQMQPQQQQQQQPQNPONPPNO   NM:i:1  AS:i:98 XS:i:19
HWI-ST115_0148:1:28:4544:44664#0    16  chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC    ;===>@ACCB?DAAABCCCE@@CFA?CBB?BE?8ACCA@DABCEABEADBCDAA??CAAB?A>@?E@>D@E?@B?@E@@?B>BDABDA?7@C><;76423    BD:Z:BBKNJKMBLMLLJJMNLBJIMKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMMLIOOPQOMLJJ   MD:Z:100    RG:Z:0.1    BI:Z:HHOQKMOIPQQQLLPRQIOMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMPNNONMOPMPOOPPQQPQQOMPOPRRPPNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:22:13125:90542#0   1040    chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC    9;;<>?@BBA>?A@>AABB;@>BD@ABBA?@D?8@BBA@D@ABB@BD@BBBDAC??C@@A@@>@BD@>B@D>AC@@DA@?B>BDABDA@7@C>;<76424    BD:Z:AAJMJKMCLMMMKJLMKAIILLMMNKAJLLMMMKKAJKNIKAHIKIIJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLKMLKINNPPPLKII   MD:Z:100    RG:Z:0  BI:Z:GGNPKMOHPRQQKKPQQHOMPPQPPPGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOQRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:28:12239:165270#0  1040    chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC    9<;;=?@BBB=B??=@B?CA@?BD??BCC?AD?7@BBA@D@BBD@BD@BBBCAC??B?@A@B>?BD@?C@D@AC@@D?@?B>BDABDA@7@C>;<88423    BD:Z:AAJMJKMCLMMMKJLMKAIILLMMNKAJLLMMMKKAJKNIKAHIKIIJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLKMLKINNPPPLKII   MD:Z:100    RG:Z:0  BI:Z:GGNPKMOHPRQQKKPQQHOMPPQPPPGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOQRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:61:10099:38446#0   1040    chr3    58125523    60  100M    *   0   0   TTTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC    ;=;;=?@BBB>CA@>ABBBD@>BD@@BBA?AD>8@BBA@D@BBD@BD@BBBDAC?@C@@@@A>?BD@>C@D@AC@@D?@?B>BDABDA@7@C><<76424    BD:Z:AAJMJKMCLMMMKJLMKAIILLMMNKAJLLMMMKKAJKNIKAHIKIIJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLKMLKINNPPPLKII   MD:Z:100    RG:Z:0  BI:Z:GGNPKMOHPRQQKKPQQHOMPPQPPPGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOQRQPPMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:21:5903:122469#0   0   chr3    58125524    60  100M    *   0   0   TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    4665=<BAAB>@>>>@BBB?A?B@?DCCB>B??8@BCAC?@BB?@B?ABBC?AA@EA>A@CBAEC@A?AA@>A@>AAAADC?CAABAA@7AA?@B>>::<    BD:Z:JJAKOLKKCLLNMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLIMLLNNMNNMLNJOPMMLNMJ   MD:Z:100    RG:Z:0.1    BI:Z:NNHORNNNHPPQOJJPPNGNMPOPQOMHOOPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQQQRRQRRQPRNRPONPQOK   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:43:7687:177083#0   1024    chr3    58125524    60  100M    *   0   0   TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    4665=<BAAB>@>>>@BBB?@>B??DBBB>B??8@BBAC?@BB?@B?@B?C?AA@EA?ABDBAEC@@?AAA>A?>?@B@DB?BAACAAA7A@?@B>>:;:    BD:Z:JJAKOLKKCLLNMIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLIMLLNNMNNMLNJOPMMLNMJ   MD:Z:100    RG:Z:0.1    BI:Z:NNHORNNNHPPQOJJPPNGNMPOPQOMHOOPQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQQQRRQRRQPRNRPONPQOK   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:28:17653:40535#0   1024    chr3    58125524    60  100M    *   0   0   TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    4665=<BAAB>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACAADB?A>@@@?AA>@?@@D??B?@?@A?7@?>@B=<:89    BD:Z:IIAJOKLJBLLMMJIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLLOMNLLMJJLLMNILMNIMKLMLIMMIMLLNMLNNNLMKPONMLMLJ   MD:Z:100    RG:Z:0  BI:Z:MMGORNNNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOPONQQMQPQQQQQQQOQOQOPNPPOJ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:6794:128447#0   1024    chr3    58125524    60  100M    *   0   0   TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    4665=<BAAB>@>>>@BBB?@?A??DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@>@ABAADB?A>@@@>A@>>?A@C??B@AB@A<7@?=@A<=<7;    BD:Z:IIAJOKLJBLLMMJIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLLOMNLLMJJLLMNILMNIMKLMLIMMIMLLNMLNNNLMKPONMLMLJ   MD:Z:100    RG:Z:0  BI:Z:MMGORNNNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOPONQQMQPQQQQQQQOQOQOPNPPOJ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:8:15977:151184#0   1024    chr3    58125524    60  100M    *   0   0   TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    4566=<B@AB>@>>>@BBB???A??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@@BAADB?@?@@@>BA>>?A@D@?B?AB@A?6@?>@B<=::7    BD:Z:IIAJOKLJBLLMMJIJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLLOMNLLMJJLLMNILMNIMKLMLIMMIMLLNMLNNNLMKPONMLMLJ   MD:Z:100    RG:Z:0  BI:Z:MMGORNNNGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOPONQQMQPQQQQQQQOQOQOPNPPOJ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:43:17533:112959#0  16  chr3    58125524    60  100M    *   0   0   TATCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    5*;;>?BBB>C@=@@BBBD@>BD?@BBB?CE>7ABB@AD@BBD@BD@BBBDAC??B@@@@A>?BD@>C@D@AC@@D?@?B>BDABB@?8?D><>:;4224    BD:Z:LLMIJMCLNMMKKLMKAIILLMMNKAJLLMMMKKAJKNIKAIHKIIK@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJMLKHNNNPOPKJII   MD:Z:1T98   RG:Z:0  BI:Z:OPPMKOGORRQKKPQQHOMPPQPQOHNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOORQRPJMM   NM:i:1  AS:i:98 XS:i:19
HWI-ST142_0240:1:67:8529:131204#0   1040    chr3    58125524    60  100M    *   0   0   TTTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCC    =;;:=>BBB?B@>:@BBBC@?BD?@BBC?CE?7ABB@AD@CBD?BD@BBBDAC?@C@@@@A<?BD@?C@A@@B=@D7@?B>BDABDA@8@D>=>:;4224    BD:Z:AJMIJMCLNMMKKLMKAIILLMMNKAJLLMMMKKAJKNIKAIHKIIK@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJMLKHNNNPOPKJII   MD:Z:100    RG:Z:0  BI:Z:GNPMKOGORRQKKPQQHOMPPQPQOHNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOORQRPJMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:64:11727:57771#0   0   chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    46599@AAA??==>ABBC?@>BA?DBBB?BA?7@CCAC?@BB??A@@C>CAAA@D??@@>@?DBAA<?>A=@>=4?>;C?9?10??@*37<*>5<@<9:>    BD:Z:JJKLLLLCMLNNIIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHMLLNNMNNMLMJOONMMNMJM   MD:Z:100    RG:Z:0.1    BI:Z:NNOPONOHPPQOJJPPOGNMPOPPPMGPOOQQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPQRRQRRQPRNRQPONQOKQ   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:26:10573:112719#0  1024    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    46589@@AA>@>>>@BBA?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ABA@EB?@?@A@>A@><?A@DB>B?AB@B@7???@?>>:77:    BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL   MD:Z:100    RG:Z:0  BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:41:15139:143134#0  1024    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    46589@@AA>@>>>@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB@@@?D@>@ACA@EB?@?@A@>A?>??A@DB>A?@B@@A7>?>@B>?:787    BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL   MD:Z:100    RG:Z:0  BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:48:9218:101715#0   1024    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    46589@@AA>@>>>@ABB??>B??DBBB>B??7@BB@C@@BB?@B?@BBB?A??D?>@@BA@EB@@?@@@>A??=@A@D@>B@@B@A>+?@??C=@;8:=    BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL   MD:Z:100    RG:Z:0  BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:64:4088:153434#0   1024    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    46589@@AA>@>>>@BBB?@>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@@?D@=@AB@@CA?@?@@@>A?>>@A?DA>B?@B@A=7??=@@>:;88:    BD:Z:IIJKLLLBLMMMJJJKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKOMNMKMJJLLMNILMNIMKLMLIMMIMLLNMLNMNLMJPONNMMLJL   MD:Z:100    RG:Z:0  BI:Z:MMOPNNPGPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOPMQQMQPQQQQQQQOPORPPPNPOJP   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:25:6245:195434#0   16  chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    ><:<>BBB?DAAABCBCEB?BF@@CCC?CE@8ACCAAEACCEACEACCBEAC??C@@@@@>?BD@>C@D@AC@@D@@AB>BDABDA@7@D?=?<>92234    BD:Z:KNIJLBMMMMJJMNLBJIMLMMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKINNOONPOJMJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQMNMGOQQQLLPRQIONQPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOMOOLOQMPOOPOPQPQQOMPOOQQQRJONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:3:18284:27273#0    1040    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    =<<=>CCB@C??>@BCBDB@BD?A@B@>BE?7@@@A?DACCE?CD@CC2D@C@@C?BA@?>?BEA?CAE?@C=@C;@AB?ACABEA@8AD@=>=>;3123    BD:Z:KNIJLBMMMMJJMNLBJIMLMMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKINNOONPOJMJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQMNMGOQQQLLPRQIONQPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOMOOLOQMPOOPOPQPQQOMPOOQQQRJONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:6:8671:12086#0 1040    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    =<:=>CCB?C??>@BBCEA?BD?@C@>6@B<7@CBB?D@>CB;CE@CC@D>C?AC?@B@??@BD@>C@D@@C=@C@AAB?@E?AC@@7?D>=><>;3123    BD:Z:KNIJLBMMMMJJMNLBJIMLMMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKINNOONPOJMJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQMNMGOQQQLLPRQIONQPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOMOOLOQMPOOPOPQPQQOMPOOQQQRJONN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:42:8160:116894#0   1040    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    ;;9;>AB@>C@>AABBAD@:AD??BA@?BD?5@BBA@E@BBD?BC@BBBDAB??C@@@@@>?BD@?C@D@AC@@DA@?B>BDABDA@7@D?=?<>92234    BD:Z:JMIILCLNNMKKMMKAIILLMMNKAJLLMMMKKAJKNIKAIIJIIKA@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLLKHMNNNOOOJMII   MD:Z:100    RG:Z:0  BI:Z:NPMMMGNQRRKKPQQHOMPPQPQPGOPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPQRRJOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:62:2822:138861#0   1040    chr3    58125525    60  100M    *   0   0   TTCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCA    ;:9;=ABB?A@@A>BBBD@9BB??@@A?@D?7@CB@@D@ABD?BC@BBBB@C??C>@@@@??BD@>B@D@@C@@D@??B?@D@ACA@8@D>=?<>92234    BD:Z:JMIILCLNNMKKMMKAIILLMMNKAJLLMMMKKAJKNIKAIIJIIKA@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLLKHMNNNOOOJMII   MD:Z:100    RG:Z:0  BI:Z:NPMMMGNQRRKKPQQHOMPPQPQPGOPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPQRRJOMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:23:11954:92523#0   0   chr3    58125526    60  100M    *   0   0   TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA    4584?>@A=@>>>ABBB?@?B??EBBB>B??7@BBAD?@BC?AC?ACCC?A@@E@?ABDB?EC@@??A@>A@>B@A@EC?CAAB@A?7AA@?C?B?:;@>    BD:Z:JJLILMDMMNNJIKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLLLNNMNNMLMIOOMNMOMJMJ   MD:Z:100    RG:Z:0.1    BI:Z:NNPMOOIPPQOJJPPOHNMPOPPONGOPOPQNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPRRQRRQPRNRQQPOOOKQN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:28:10887:136134#0  1024    chr3    58125526    60  100M    *   0   0   TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA    4584=>@A=@>>>@BBB?@>A??DBBB?B??7@BBAC?@BB?@B?@BBB?A@?D@?@ABA@DC?@?A@??A??7@A@DA?B?@B@@@8@?>@B?A>;;?=    BD:Z:IIKHMLDLMNMJJKKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNMNMLLJJLLMNILMNIMKLMLIMMIMLLNMLNMMLMJOONNNNLJLI   MD:Z:100    RG:Z:0  BI:Z:MMPLNPIPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOONPQMQPQQQQQQQOPNRQQPPNOJPN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:46:9571:119534#0   1024    chr3    58125526    60  100M    *   0   0   TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA    4564=>@A=@>>>@BBB??>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@??D@>@@CA@DC?@>@@?>A@>=@A@BB???@B?B@8@?>>C>A=98;9    BD:Z:IIKHMLDLMNMJJKKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNMNMLLJJLLMNILMNIMKLMLIMMIMLLNMLNMMLMJOONNNNLJLI   MD:Z:100    RG:Z:0  BI:Z:MMPLNPIPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOONPQMQPQQQQQQQOPNRQQPPNOJPN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:7:7761:36811#0 1024    chr3    58125526    60  100M    *   0   0   TCACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA    3375>>?@=?<:<8?AA=?>??=BABB;A@?7@BB>A?AB?=????@@@?A@?C@>@@B@>CB@@?@>@>A@?9@A?D@>9@AA:A>5@-<<@=>=97;9    BD:Z:IIKHMLDLMNMJJKKI@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNMNMLLJJLLMNILMNIMKLMLIMMIMLLNMLNMMLMJOONNNNLJLI   MD:Z:100    RG:Z:0  BI:Z:MMPLNPIPOPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOONPQMQPQQQQQQQOPNRQQPPNOJPN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:8305:80246#0    16  chr3    58125526    60  100M    *   0   0   TCACAAATAGGAGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAA    8::;>AA?B=?/>ACCD@?@B>?@@:?BD?7@BB@@D;@BB=AB>BCBD@C??C>?A?>>@BD@>C@D@>C=@D??=B?@D@BD??8@D>=?>;=82353    BD:Z:MIIKBLNNNMKKNKAIILLMMNKAJLLMMMKKAJKNIKAIIKHIKAAHJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKKHMMNNMONNMKII   MD:Z:11G88  RG:Z:0  BI:Z:PMMOENPQRQOOQQHOMPPQPQPHNQOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPORRLOOMM   NM:i:1  AS:i:95 XS:i:19
HWI-ST115_0148:1:41:5881:8051#0 0   chr3    58125527    60  100M    *   0   0   CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    4848=>@=?>>>@BBB?@>B??DCCB>B??7@BBAC?@BB?AB?@BCC?A??EA>ABDBAECAA?@AA>A@?@@@@DB?BA@B@A@5A@?=A>A3=:=;<    BD:Z:JJIIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB   MD:Z:100    RG:Z:0.1    BI:Z:NNMMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:43:18815:159969#0  1024    chr3    58125527    60  100M    *   0   0   CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    483:;?@=@>=>@BBC???B@?DACB?B?@7@BBAC?ABC?@B?ACBC?@??E@>A@C@ABB@A?AA@?A@>:@A@FB?BA@B@A=7A@?8>0<1<;<>;    BD:Z:JJIIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB   MD:Z:100    RG:Z:0.1    BI:Z:NNMMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:45:18848:163363#0  1024    chr3    58125527    60  100M    *   0   0   TACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    4248;>@=?>>>@BBB?@>B??DBCB>B??7@BBAC?@BB?@B?@BBB?A@?EA>ABCB?DC@A?AA@?A@??A@@DB?BAABAA@5A@??B?B?>;=>=    BD:Z:JJMIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB   MD:Z:0C99   RG:Z:0.1    BI:Z:NNPMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH   NM:i:1  AS:i:99 XS:i:19
HWI-ST115_0148:1:62:10186:37536#0   1024    chr3    58125527    60  100M    *   0   0   CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    3739>=?===99-<;??=<=?4C>BB>B??7ACA@@@ABCA@B@A>?<9@?>C>.;;>:9=B@A??;@=A@?;?@:B?<?@???@94<@7>B.@>::>3<    BD:Z:JJIIMENMONJJKLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKLNNMNNMLMINOMMNONJMJB   MD:Z:100    RG:Z:0.1    BI:Z:NNMMPIQPQOJJPPOHOMPOPPOMHOOPPPNQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQRQRRQPRNRQQQPPMKQNH   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:7:16791:111675#0   16  chr3    58125527    60  100M    *   0   0   CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    ;;:?BB>DAAA@BCCEA?CE@@CCC?CE?8ACCAAEACCFACEACCCEAD??C@@A@@<?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@=A>:73664    BD:Z:IJKALMNMKKMNLBJIMLMNNJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMNNNMNNMPKAJJ   MD:Z:100    RG:Z:0.1    BI:Z:MNOHMOPQLLPRQIONQQRPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPNLPPMQOOPOPPOQQOMPOOQPPQKQOHNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:25:19405:133414#0  1040    chr3    58125527    60  100M    *   0   0   CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    685<=@?B>>89BBBC??A>>>><??AC?7=BB@@D@B@D@CD@CBBD@B??B@@?=A8<?D@?A>D>@B>?C;?>=<4@?@B>?8?D==>=@<:85543    BD:Z:IIKAKNNNLKMNLAIILLMMNKAJLLMMMKKAJKNIKAIIKIHKAAIJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJHMMMNMMNMQKAII   MD:Z:100    RG:Z:0  BI:Z:MMOGLPPQLLPQQHOMPPQPQPHOPPPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPRLQOGMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:42:13567:43693#0   1040    chr3    58125527    60  100M    *   0   0   CACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAA    999=?A?B>@>@BBBD@?BB?@BBB?BD?8@BB@@D@CBD@BD@BBBD@C??B@@A@@>?@C@?C@D@@C@@B?@?B?BC@BD@@8?D?=?=>>:73554    BD:Z:IIKAKNNNLKMNLAIILLMMNKAJLLMMMKKAJKNIKAIIKIHKAAIJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJHMMMNMMNMQKAII   MD:Z:100    RG:Z:0  BI:Z:MMOGLPPQLLPQQHOMPPQPQPHOPPPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPRLQOGMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:46:19063:128461#0  0   chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    4486;><?=>>@BBB?@>B??DBBB>B??8@BBAD?@BB?@B?@BBB?@@@DA>ABCBAEC@A?@A@>A@>@@A@EC?CAABAA@7AA??C?C?@?@<>;    BD:Z:JJIJEONOOJJLLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKNNMNNMLMINNMMMPNKMJBK   MD:Z:100    RG:Z:0.1    BI:Z:NNMNJQQQOJJPPOHONPOPPOMGPOOQPMQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQQRRQPRNRQQQQQNIQNHO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:48:14037:187991#0  1024    chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    3488<><>====ACC;;<=>?CC@A?B@?7@CC@CA@CCAACA@CCCAA>?D?+A@C?>D?@>;>@A=@?.8/96A==@=A?<A@7???>C?B?:=@<>9    BD:Z:JJIJEONOOJJLLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKNNMNNMLMINNMMMPNKMJBK   MD:Z:100    RG:Z:0.1    BI:Z:NNMNJQQQOJJPPOHONPOPPOMGPOOQPMQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQQRRQPRNRQQQQQNIQNHO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:67:3978:70373#0    1024    chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    4486;><?=>>@BBB???B??DBBB?B??7@BBAC?@CC?@B?ABBC?A@@F@>A@CB?DC@A?AAA>@@?@A@@EB?BAAC@A@7@A??C?C@?=8=>:    BD:Z:JJIJEONOOJJLLJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKNNMNNMLMINNMMMPNKMJBK   MD:Z:100    RG:Z:0.1    BI:Z:NNMNJQQQOJJPPOHONPOPPOMGPOOQPMQNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQQRRQPRNRQQQQQNIQNHO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:26:3317:7675#0 1024    chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    2/84:809869:<5=>?<;>=BA=A;>==6???>A@@B?AAAA>B?@@B9>B>;?@A@?A@@?<?@@=A?:AAB@;@><=<BAB%%%%%%%%%%%%%%%%    BD:Z:IIIIENONNKJKLJ@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMMLMJILLMNILMNIMKLMLIMMIMLLNMLNMMKLJONMNNONKLIAK   MD:Z:100    RG:Z:0  BI:Z:MMLNIRQPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPQLQPQQQQQQQOPNQPRRQPOHPNGN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:27:5617:148989#0   1024    chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    4486;><?=>>?BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@>@ACA@DB?A>@@@>@A>A@A@EB>B?>B@@@7@@?@C>BA?>>=89    BD:Z:IIIIENONNKJKLJ@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMMLMJILLMNILMNIMKLMLIMMIMLLNMLNMMKLJONMNNONKLIAK   MD:Z:100    RG:Z:0  BI:Z:MMLNIRQPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPQLQPQQQQQQQOPNQPRRQPOHPNGN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:4:19975:33455#0    1024    chr3    58125528    60  100M    *   0   0   TCAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGAGCCTATCCCAAAC    4586;=<?=>>@BBB??>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?A>@@@>AA>9AB@DB?B?<A@B@81@==?=>=<<><<9    BD:Z:IIKIENONNKJKLJ@JHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMMLMJILLMNILMNIMKLMLIMMIMLLNMLNMMKMMPNMNNONKLIAK   MD:Z:0A85T13    RG:Z:0  BI:Z:MMPNIRQPOJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPQLQPQQQQQQQOQPRPRRQPOHPNGN   NM:i:2  AS:i:94 XS:i:19
HWI-ST115_0148:1:44:8541:127472#0   16  chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    :;=?@>CA???BCCDA?CD@?CCB?CE?7ACCAAEACCEACE@CCCEBC@?C@@A@A>?BD@>C@D@AC@@D@@?B>BDABDA@7@D?>@>A?<:86644    BD:Z:JKAKMMNKKNNLBJIMLMNNK@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMNNLNMLPNAKJJ   MD:Z:100    RG:Z:0.1    BI:Z:NOHONOPLLPRQIONQQRQQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOLOQMPPOPOPPOPQOMPOOQPPPKPQHNNN   NM:i:0  AS:i:100    XS:i:19
HWI-ST142_0240:1:68:18974:109354#0  1040    chr3    58125528    60  100M    *   0   0   ACAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAAC    89;4?=A==>*?B@A2/=?(.416;-::1/AB@?B,9=B<4147/>8>A;:A98?<?(=?C@?B?D@@B>@A@@?B?BB@BD??8>C:<<>??;;86523    BD:Z:IKAJMNNLLMNLBIILLMMNKAJLLMMMKKAJKNIKAIIKIIJAAIKILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGMMMMMMLMPOAJII   MD:Z:100    RG:Z:0  BI:Z:MOGNNPPKLQQQHOMPPQPQPHOQOQOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPLQQGMMM   NM:i:0  AS:i:100    XS:i:19
HWI-ST115_0148:1:25:1660:58913#0    0   chr3    58125529    60  100M    *   0   0   CTAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGGGACATATGATGACGTGCCTATCGCAAAAA    3729<<?==>@@BCA9;;=8DA=A<B@?7ACA@C@@CC?AC?ACCC<A??E?<@@C??DB@A?@A@>A??%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJLJOOPOKJLMJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMIIKLHLKKMNMNNMLMINNLMMONOOJBBB   MD:Z:1A68T22C4C1    RG:Z:0.1    BI:Z:NNPNRQROJJPPOHONQOPPOMGOPOPQMPNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOJJPPMQPPQQPRRQPRNRQQQQROONNHHH   NM:i:4  AS:i:86 XS:i:20
HWI-ST115_0148:1:27:6860:74605#0    1024    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    3768=:<;=>>>BB@<>A@?ECAB?BA@8@CC@CA@CC@@C?@DCC?@@?EB<@AEA>DB@A?@@@=@@>9?9>@B?B@ABA==5A@=<B;B@?=D?>;>    BD:Z:JJJBOOPOKJLMJ@JILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMNMNNMLMINNLMMOOKNJBKI   MD:Z:100    RG:Z:0.1    BI:Z:NNNHRQROJJPPOHONQOPPOMGOPOPQMPNHOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPRRQPRNRQQQQROJONHOM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:26:3714:23222#0    1024    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    4766;:>==>@BBA??>A??DBAB=B@=86BA@C@@BB?@B?@BBBA@@?A@?A@BA>DB@@?@@@>A@<=<A>CA?B?@<?A57;?>>A=B)?=2><6:    BD:Z:IIIAOOPNKKKLJAJHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLLMJJKLMNILMNIMKLMLIMMIMLLNMLNMMKLIONMMNONLMIAKI   MD:Z:100    RG:Z:0  BI:Z:MMNGRQROJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPMPPQQQQQQQOPNQPQRRQOJNNGNL   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:27:16183:58921#0   1024    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    4867;;>===@@BA??>@??DBAB><@?8@BB>B?@BB?@B?@BBB?A@>D?>A@C@>BAA@??@?>B?>8)9=>A?A=A9AA@5;@=>@>B@==A>;79    BD:Z:IIIAOOPNKKKLJAJHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLLMJJKLMNILMNIMKLMLIMMIMLLNMLNMMKLIONMMNONLMIAKI   MD:Z:100    RG:Z:0  BI:Z:MMNGRQROJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPMPPQQQQQQQOPNQPQRRQOJNNGNL   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:4:9939:78105#0 1024    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    4856;;====@ABB???A??DBBB>B@?7@BB@A?@BB?@B?@BBB?@@?D@>@ACA@DB?@?@@??A@<>A??AB?B?A9?@@8>@?<?>B>:=@=;7:    BD:Z:IIIAOOPNKKKLJAJHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLLMJJKLMNILMNIMKLMLIMMIMLLNMLNMMKLIONMMNONLMIAKI   MD:Z:100    RG:Z:0  BI:Z:MMNGRQROJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPMPPQQQQQQQOPNQPQRRQOJNNGNL   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:2:3326:122977#0    1040    chr3    58125529    60  6S94M   *   0   0   CGATCTCAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCC    %:<>=A?B@@=B>>:?BBC?9=?B<BC<B=@D<7<CCA@E<?CD<>><@?CC@A?@@?<??A>ABE=?C>E?@@?@E@@A>=?A8<D>=6=@=;;98523    BD:Z:LNMKMOMBLMLLJJMNLBJIMKLMMJ@JKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMMLIOOPQOMLJJ   MD:Z:94 RG:Z:0.1    BI:Z:OQPONPOIPQQQLLPRQIOMPPQPQOHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMPNNONMOPMPOOPPQQPQQOMPOPRRPPNN   NM:i:0  AS:i:94 XS:i:19
HWI-ST115_0148:1:2:7831:17632#0 16  chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    ;>;>=C<>?;BA?D@?BD=@<8A/@D?7@A,A>D@@BC4:??CBBE@??AA=>;=A>A>C@?A@D?@C?@A=>@A>BB@BA??4;D<;=<A<<;<>8433    BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:43:1461:170805#0   1040    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    ;><>=C=>?@@2CFA?BE@@CBA?BE?7@BCAAE@CBE@CD@CCCEBD?AC?@??A>?BE@>C@E?AC@@EA@@B?BEABD@@8@D?=@?B?=<;;6433    BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:5:8019:89787#0 1040    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    ;>=>=C??>@@A<D5?BD?@CBB?BD)7@CCBAE@@AA@CD@CCBE@D?@C?AA?A>ABD@>CAD?AC@@DA@?B?BE?BEA@7@E?=A?B@=<;=6434    BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:63:8805:141247#0   1040    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    ;>=>>CAA?BCCCEA?BE@@CCC?CE?8ACCAAEACCEACEACCCEBD?@C@@@@A>?BD@>C@D@AC@@DA@?B?BEABDA?7@D?>@>B?=<;;6434    BD:Z:KAKLMMLKNOLBJIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMNLMMKONDKJJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHOPNOKLPRQIONQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMOPNPOPPOPPOPPOMPOOQPPPJPPJNNNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:7:2654:174038#0    1040    chr3    58125529    60  100M    *   0   0   CAAATAGGTGATCTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGCCATATGATGACGTGCCTATCCCAAACA    %%%%%%%%%%%%%%%?BD?@?9@>AB=63@39:-./;D;BB>:BB=:><>@';>=A9>BD>>C@E=8B:?A9'7=:<>;:C??6?=8:<8>6:<::9532    BD:Z:KAKLMMOJMOMKOIMLMNNKAJKKLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHMMLMKKLKMLKMLKHMMMNLMMKONDKJJJ   MD:Z:8G3T59A27  RG:Z:0.1    BI:Z:OHOPNOONQRQPRNQQRQROHOPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMONOPOPPOPPOPPOMPOOQPPPJPPJNNNN   NM:i:3  AS:i:85 XS:i:19
HWI-ST142_0240:1:2:17083:137881#0   1040    chr3    58125529    60  100M    *   0   0   CTAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    ;7<=<A@>@ABBCD@?BC??BBB>CD@7>CB@@E@BBE@BC@BBBDAC??C@@@@A>?BD@>C@D@AC@@DA@?B>BDABDA?7@D?>@>B?=<;;6434    BD:Z:MJJLMNLLNNLBJILLMMNKAJLLMMMKKAJKNIKAIIKIIK@AIKJLMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLMMMLMLKPNEJIII   MD:Z:1A98   RG:Z:0  BI:Z:PPNPNPJKQRQHOMPPQPQPHOQPPPONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJQQIMMMM   NM:i:1  AS:i:98 XS:i:0
HWI-ST142_0240:1:42:16611:43608#0   1040    chr3    58125529    60  100M    *   0   0   CAAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACA    4<<==A@@@ABBBD@>AD??B@A?BC@8ABB@@D@@BC@CD@BBBD@C?@B>@B@@=??D@?C@D>>C@@C?@>?=BB@@DA@8@D?>@>B?=<;;6434    BD:Z:KAJLMNLLNNLBJILLMMNKAJLLMMMKKAJKNIKAIIKIIK@AIKJLMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLMMMLMLKPNEJIII   MD:Z:100    RG:Z:0  BI:Z:OGNPNPJKQRQHOMPPQPQPHOQPPPONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJQQIMMMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:23:11369:77134#0   0   chr3    58125530    60  100M    *   0   0   AAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAG    46667<<==@BBB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ABA@DB?@>@@@>A?>@@A@CA?B?@B=?@7@@?@B<A@>@B@=;;6    BD:Z:IIAKPPPKKLLJAKHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKMJJLKMNILMNIMKLMLIMMIMLLNMLNMMKLINNMMMONLNJAKIN   MD:Z:100    RG:Z:0  BI:Z:MMGPQRQJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLQOQQQQQQQOPNQPQQRRPJPLGNLP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:65:18149:144308#0  1024    chr3    58125530    60  100M    *   0   0   AAATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAG    46668<<==@BBB?@>B??DBBB>@??8@BBAC?@BB@@B?@BBB?@@?D@>@@CA@DA?@>A@?>A?>@@A@CB?B?@B;A@7@@?A@=BA@@B@=:>9    BD:Z:IIAKPPPKKLLJAKHKKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKMJJLKMNILMNIMKLMLIMMIMLLNMLNMMKLINNMMMONLNJAKIN   MD:Z:100    RG:Z:0  BI:Z:MMGPQRQJJPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLQOQQQQQQQOPNQPQQRRPJPLGNLP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:64:2167:161953#0   0   chr3    58125531    60  100M    *   0   0   TATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGT    42629:<=>BBB?@?B??EBCB<B??7@CB@C?@BC?@C?@CCC?A@?C?>@@C@ADBAA??A@>A??>A?@9B;AAAB@?37;B?>B:A>9>C.@;>6;    BD:Z:JJLLQQLKMMKAJILKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLNNMLMINNLLLONKOKCKION   MD:Z:0A99   RG:Z:0.1    BI:Z:NNPPSPKJPPOHONQPQPOMGOOOQPMQMGOOPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQQPRNRQQQQRPLQMFOMQQ   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:44:10831:41289#0   16  chr3    58125531    60  100M    *   0   0   AATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGT    ;<8=<?=@BBBD@?BD??B@B?BB?8@BBA@D?BBC@BC@BBCD@B>?C@@A@@>?@D@?B@D@@C@@C@@?B?BD?BD??8?D?=@>B@<<>?>:6623    BD:Z:JLLLKLNOMBJJMLMMNKAJLLMMMKKAJKNIKAIIKIIKAAHKJMNLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLMLLKKNLDMMMLII   MD:Z:100    RG:Z:0  BI:Z:NPPPHJOQRIOMPPQPQPHOQPQPOONGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOIOOPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:4:12425:123146#0   1040    chr3    58125531    60  100M    *   0   0   AATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGT    7=9>>=@AAABD@>BD??BBA?BD>7=BBB?D@BAD@BD@BBBD@C??C@@A@@>@BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>=>@>93744    BD:Z:JLLLKLNOMBJJMLMMNKAJLLMMMKKAJKNIKAIIKIIKAAHKJMNLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLMLLKKNLDMMMLII   MD:Z:100    RG:Z:0  BI:Z:NPPPHJOQRIOMPPQPQPHOQPQPOONGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOIOOPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:2:16506:172864#0   0   chr3    58125532    60  100M    *   0   0   ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCTAACAGTC    342688.<=<;;;4>@=ABB>=>@=6?BA=B@@B=@@@>?BABA<=>D>?A@C@=B?A@>>@@<A@>@@>;C6>?8;;>9*-7415>,58=>06@<>988    BD:Z:IILMQMMLMKAKILKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIJLLMMILMNIMKLMLIMMIMLLNMLNMMKLINMLMMNMLNNLLINNM   MD:Z:92A7   RG:Z:0  BI:Z:MMOPQLLPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOQPQQQQQOPNQPQQQQQLQPNLLPQP   NM:i:1  AS:i:95 XS:i:0
HWI-ST142_0240:1:68:15909:199980#0  1024    chr3    58125532    60  100M    *   0   0   ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC    46257:<?ABB?@>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@?@A?>A@>?@A@DB>B@@B?@@7???@C?B@@>=BA>?::9    BD:Z:IILMQMMLMKAKILKMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIJLLMMILMNIMKLMLIMMIMLLNMLNMMKLINMLMMNMLNKCLINNM   MD:Z:100    RG:Z:0  BI:Z:MMOPQLLPPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOQPQQQQQOPNQPQQQQQLQNGLLPQP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:65:7341:14563#0    16  chr3    58125532    60  100M    *   0   0   ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC    ;:9;@?ABCCEA?CE@@CCC?CF@9ACCA@EACBEA@EACCCEBD@@D@AA@@>?BD@>C@D@AC@@D@@?B>BDABDA@7@E?>@>B@>>>A@<87444    BD:Z:LLLJKNPMCKIMLMNNKAKLLLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLLJMLBMMQLMJJ   MD:Z:100    RG:Z:0.1    BI:Z:PPPKINQQIONQQRQRPIPPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNPOPPOQPOPPNLOOOQPPPJOOHOORPPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:68:10363:186753#0  1040    chr3    58125532    60  100M    *   0   0   ATAGGGGATTCGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC    <:>;>=?BCA5@?BE?@CCB?@E?7@CCA@D@@CD>;D@CB=D@C?AC=@@@A>ABE@?C@E?@C?@E@@@B?BC@AE?@7@D>=>?A@<=@A@<;8343    BD:Z:LLLJKNPMLOLMLMNNKAKLLLMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLLJMLBMMQLMJJ   MD:Z:10T89  RG:Z:0.1    BI:Z:PPPKINQQPRPQQRQRPIPPPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNPOPPOQPOPPNLOOOQPPPJOOHOORPPNN   NM:i:1  AS:i:95 XS:i:0
HWI-ST142_0240:1:21:15621:181493#0  1040    chr3    58125532    60  100M    *   0   0   ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCTAAACAGTC    77<;;=A?BBD@?BD??BBB?BD?7@BBA@D@BAC@CB?BBABAC?@B@@A>A>A@B@>C@D>AC@@D@@?B?BAABC@?8@D=>??@A=?<A@<87334    BD:Z:LLLJKNOMCJJMMMMNKAJLLMMMKKAJKNIKAIIKIIKAAIJJMNMKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLLLKMNKBMLQLMII   MD:Z:91C8   RG:Z:0  BI:Z:PPPJHOPQIPMPPQPQPHOQPQPPNOGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPOPPGOORPPMM   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:25:4337:107457#0   1040    chr3    58125532    60  100M    *   0   0   ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC    =9=;>=ABBAD@?BD??BB<?BD?7@BCA?D@C=D@CE@BBBDAC?@B@@A@@>@AD@=C@D@AC@@D@??B>BDABDA@7@D?>@?A@>>>A@<87444    BD:Z:LLLJKNOMCJJMMMMNKAJLLMMMKKAJKNIKAIIKIIKAAIJJMNMKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLLLKJNLBMLQLMII   MD:Z:100    RG:Z:0  BI:Z:PPPJHOPQIPMPPQPQPHOQPQPPNOGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGOORPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:6:20790:48630#0    1040    chr3    58125532    60  100M    *   0   0   ATAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTC    79<;<=@?@AD@?@D??BB?=BD?7?BBA?C>CBC?@A>CABC=B?@B>@A@A>?@D@=B@D@@C@@D@@?B>BDABCA@7@D?>@>B?>=>A@<87444    BD:Z:LLLJKNOMCJJMMMMNKAJLLMMMKKAJKNIKAIIKIIKAAIJJMNMKKLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLLLKJNLBMLQLMII   MD:Z:100    RG:Z:0  BI:Z:PPPJHOPQIPMPPQPQPHOQPQPPNOGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGOORPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:48:10313:163996#0  0   chr3    58125533    60  100M    *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC    42427:>AAB?@>B??DBCB?B??7@BB@A?@BB?@B?@BBB?B??E@>ABCB?DB@A>@A@?B==;B@?DB=@AB=:A?5A@??C?B@??CCC@B=;;7    BD:Z:JJNNMMNNLAKJLKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMMLMINNLLLNMKNKDLJONNM   MD:Z:100    RG:Z:0.1    BI:Z:NNQOLKQPOHONQPQQPMGOOOPPNPMHNNPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPORNRQQQQRPLROHNKQQQO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:61:13700:181997#0  1024    chr3    58125533    60  100M    *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC    42427:>AAB?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?B@?E@>@@CBACC@A?@AA=A@>@@A@DB?BAABAA@7AA?AC?B@A??@C@B<;;8    BD:Z:JJNNMMNNLAKJLKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMMLMINNLLLNMKNKDLJONNM   MD:Z:100    RG:Z:0.1    BI:Z:NNQOLKQPOHONQPQQPMGOOOPPNPMHNNPNNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPORNRQQQQRPLROHNKQQQO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:23:10492:110769#0  1024    chr3    58125533    60  100M    *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC    42427:>AAB??>A??DBBB>B??8@BBAC?@BB?@B?@BBB?@@?D@>@@CA@DB?@?@@@>A@><@A?CB<A?AA@A?.@@?@<??@=@DBB?@;886    BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMKLINMLLMNMKNKCMJNNML   MD:Z:100    RG:Z:0  BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQOPNQPQQQQPLROGNJPQPO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:43:9276:78825#0    1024    chr3    58125533    60  97M3S   *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACTTGCCTATCCCAAACAGCAC    42427:>AAB?>>B??DBB@>B??7@BBAB?@BB?@B?@BBB?@@?D>>@ABA@DB?@>@A?>B@>:?>?C@>@@B>@A32:=:=C=B>==BBB>%%%%%    BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMLIKNMLLMNMKNKCMJNOMH   MD:Z:80G16  RG:Z:0  BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQQOPQPQQQQPLROGNJPQOL   NM:i:1  AS:i:92 XS:i:19
HWI-ST142_0240:1:68:5786:48821#0    1024    chr3    58125533    60  100M    *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC    42427:>?AB?>>A?=DBAB>B?>7@BBAC??BBA@B@ABBB??@?D@;@@C@@DB@@>@@@>B@>2A;>C>>@@AB@B@7@?>@B?C>@>B@C9@:955    BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMKLINMLLMNMKNKCMJNNML   MD:Z:100    RG:Z:0  BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQOPNQPQQQQPLROGNJPQPO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:8:20535:125701#0   1024    chr3    58125533    60  100M    *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC    42427:>AAB>>>A??DBBB>B??7@BB?C@@@>@@A@?BBA?A@?D@>@ACA@DB?@>@@?>A@><?A@CB?B@AB?@@4@??@C?B@=>DBC?A:985    BD:Z:IIMMNMNMKBKILLMMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIILLMNHLMNIMKLMLIMMIMLLNMLNMMKLINMLLMNMKNKCMJNNML   MD:Z:100    RG:Z:0  BI:Z:MMPOLLRPNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPQPQQQQOPNQPQQQQPLROGNJPQPO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:41:6715:44727#0    16  chr3    58125533    60  100M    *   0   0   TAGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCC    8>97>@BC@DA?CF@@CCB?BD?7ACCA?EACCEABE@CCCEBC@@CA@A?<?ABE@?C@E?AD?@E@>@B?BDABDA@7@D?>>>B@>>?BA>=<5414    BD:Z:LLJJNONCKJMLMNNKAKLLMMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKJMKBLLQOMLJJ   MD:Z:100    RG:Z:0.1    BI:Z:PPKKMPPIONQQRQRPIPQPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQOOQOPQOPPNLONOQPPPJOOHNOQRPPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:26:15826:129753#0  0   chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC    22269<?AA??:<><:;;>6:;53;;>:8?ACB@AC@AB?0:?;4C;9@?C@?DAA;>??</@?:8??9;A=<>@B@%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJNJMOOLBKJMKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLLMINNLLLNMJNKCMJPNNMJ   MD:Z:100    RG:Z:0.1    BI:Z:NNOJLQQOHONQPQQPNGOOOPPMQMGONONNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQNRQQQQRPLROIOLOQQOK   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:7:13526:193281#0   1024    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC    44227<@AA???B??EBBB>B??7@BBAC?@BB?@B?@BBB?@@@D@>@ADAAEC@@?@A@?A??>AA@DB?B@ACAAA8A@@AD?CB?AECCAC==::7    BD:Z:JJNJMOOLBKJMKMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLLMINNLLLNMJNKCMJPNNMJ   MD:Z:100    RG:Z:0.1    BI:Z:NNOJLQQOHONQPQQPNGOOOPPMQMGONONNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQNRQQQQRPLROIOLOQQOK   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:23:17158:177951#0  1024    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC    44227<@AA>@>@??DBBB?B?>7@BBAC?@BB?@B?@BBB?A@?D?>@@C@@DB?@>@@?>A@>A@A@EA?@AAB@A@7;@?@C>B@@@CBB@C><985    BD:Z:IIMJNNOKBLILLNMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKLMNIKMNIMKLMLIMMIMLLNMLNMMKLINMLLLNMKMKCMKONMLJ   MD:Z:100    RG:Z:0  BI:Z:MMOJLRRNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPQPQQQOPNQPQQQQPKRPHNLNQPOJ   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:23:9097:197994#0   1024    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCA    44227<@AA?@>B??DBBB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@@CA@DB?@?@@@>A?>A@@@DB?B@AB?A@8@@?@;?B@>=DBB@B==88:    BD:Z:IIMJNNOKBLILLNMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKLMNIKMNIMKLMLIMMIMLLNMLNMMKLINMLLLNMKMKCMKONMLL   MD:Z:99C0   RG:Z:0  BI:Z:MMOJLRRNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPQPQQQOPNQPQQQQPKRPHNLNQPOP   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:45:14120:173983#0  1024    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCCAACAGTCCC    32158<<@@?><>;=CAAA>@@=6?AA@>@@BB@AB@@BBB@A@?D@>@@B@>DB@@?@@8>A?<8@<>CA?B@A?@A>6;A=>B=@,==)BB;;=;867    BD:Z:IIMJNNOKBLILLNMLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKLMNIKMNIMKLMLIMMIMLLNMLNMMKLINMLLLNMKKNKMKONMLJ   MD:Z:90A9   RG:Z:0  BI:Z:MMOJLRRNGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPQPQQQOPNQPQQQQPKLRONLNQPOJ   NM:i:1  AS:i:95 XS:i:0
HWI-ST115_0148:1:44:14880:140203#0  16  chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC    =:9;??@CDA?CE@@CCC?CE@8ACCA@D@CCCACEACCCEBD@@DAAAA@>?BD@>C@D@AC@@DA@?B>BDABDAA7@D@>@>B@>>?BA?=?94224    BD:Z:LJJMOMDKJNLMNNKAKLLMNMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKIMKALKPOPLJJJ   MD:Z:100    RG:Z:0.1    BI:Z:PKKOOOHONQQRQRPIPQQQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPOPPPPPPPNLONNQPPPJOOHNNQQRPJNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:46:16430:16956#0   1040    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC    =:82>AC=D@=BEA@CCB?BD@7ACCAAE@CCDACD@CCBDBC@AC?@AAB=@BD@?C@C@AC@@E@@AB>BDABDA@7@D?=?>BA>>?BA>=?:6223    BD:Z:LJJMOMDKJNLMNNKAKLLMNMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKIMKALKPOPLJJJ   MD:Z:100    RG:Z:0.1    BI:Z:PKKOOOHONQQRQRPIPQQQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPOPPPPPPPNLONNQPPPJOOHNNQQRPJNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:64:9439:169187#0   1040    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCA    =;99@BCCEA?BE@@CCC?BE@7@CCAAEA@CEABEACCCEBC@ADAAAA@>?BE@?C@D@@C@@CA@?B>BD@BD@@7@D@=@>BA>>?BA?=?;6234    BD:Z:LJJMOMDKJNLMNNKAKLLMNMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKIMKALKPOPLMJJ   MD:Z:99C0   RG:Z:0.1    BI:Z:PKKOOOHONQQRQRPIPQQQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPOPPPPPPPNLONNQPPPJOOHNNQQRPONN   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:26:17469:23074#0   1040    chr3    58125534    60  100M    *   0   0   AGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCC    9769<@AAD@?BD>?@B>?@B>7?BBA@D?ABC?@D@BBBA@C>?A>@B@A>@<C??C@D@@B@?D@A@B>BDABA@?7@D?>@=@@>=?B>?=?94114    BD:Z:LJJLNMCKKMMNNNKAJLLMMMKKAJKNIKAIIKIIKAAIKJLNMLLLJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKKJMKBKJPOQKJII   MD:Z:100    RG:Z:0  BI:Z:PJJONPGONQPQPQPHOQPQPPOOGOOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMRRRPJMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:67:21313:129109#0  0   chr3    58125535    60  100M    *   0   0   TGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT    4323;>@A>@>B??EBBB?B??7@CBAC?@BB?@B?@CBB?A@?E@>A@CB?CA@A??A@>B@??A@8AA:BABBA@@7AA?>C?B@??DCC@D=><93=    BD:Z:JJMJOPMBLJMLMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKMINNLLLNMJMKCLKPONMJL   MD:Z:0G99   RG:Z:0.1    BI:Z:NNOJRQPHONQPQQPNHOOOPPMPNGNOOMNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMRQQQQRPLROIPMPOQOKP   NM:i:1  AS:i:99 XS:i:0
HWI-ST115_0148:1:7:21244:71724#0    1024    chr3    58125535    60  100M    *   0   0   TGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT    4312;>@A>@?@@@EBBB?B??7@CBAC?@BB?@C?@CCC@A@?E?>AACB?BC@A??A@?B=?>@A@?B?@AB<AB97@A>>C?B>7>DCC@E<?<8:=    BD:Z:JJMJOPMBLJMLMMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKMINNLLLNMJMKCLKPONMJL   MD:Z:0G99   RG:Z:0.1    BI:Z:NNOJRQPHONQPQQPNHOOOPPMPNGNOOMNHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMRQQQQRPLROIPMPOQOKP   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:27:7230:150558#0   1024    chr3    58125535    60  100M    *   0   0   GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT    42229>@A>@>B??DBBB>B??7@BB@C?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>AA>@@A@DB?C@AB@@@7@??@C>?A>@DBB@D?>;;;9    BD:Z:IIJJOOMBLJLLNNLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKMNILLNIMKLMLIMMIMLLNMLNMMKLINMLLLMMKMJCMKPOMLJL   MD:Z:100    RG:Z:0  BI:Z:MMJJRRPGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPQPQQOPNQPQQQQPKQPIOLPOPOJP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:46:14388:190521#0  1024    chr3    58125535    60  100M    *   0   0   GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT    4222;>@A>@>B??DBBB>B??7?BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@??>A?>@?A@CC?C@B??A@7@=?@A>A@=>DBB@D>><;;;    BD:Z:IIJJOOMBLJLLNNLH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKMNILLNIMKLMLIMMIMLLNMLNMMKLINMLLLMMKMJCMKPOMLJL   MD:Z:100    RG:Z:0  BI:Z:MMJJRRPGOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPQPQQOPNQPQQQQPKQPIOLPOPOJP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:41:6701:64831#0    16  chr3    58125535    60  100M    *   0   0   GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCA    99.7=BCA@=;D?@C/B<BD?7<B@A@D>8CE@?E@CCCD@@?@@?@?=@>ABC@<C@B>=A<3B@?@>?@C<?A?>7@E=>>=@A<=?@B=>@:;4243    BD:Z:JJMNMCLJNMMNNKAKLLMNNKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILKAKKONPOJMJJ   MD:Z:99T0   RG:Z:0.1    BI:Z:KKOQNGNNQQRQRPIPQQRPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPPOQPOQPNLONNPPPPJOOHNNPQQRJONN   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:45:15962:97980#0   1040    chr3    58125535    60  13S87M  *   0   0   GCTCTTCCGATCTGGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATA    8:;;?@>5@A?@D@>?@BABD@>BE?@BBA>AC?7:BBA@D@ABB@BB@BBBBAC?@B@=@@@><>D@>C?D@AC>@CA??B>BDABD??7?C9::2624    BD:Z:OLKJKMNMOMLOMJJLMKAIILLMMNKAJLLMMMKKAJKNIK@IIKIHJ@@HJILMLKKLJKJLMLHKKKGKKIKKGJLHMKKLJLMKMLLINPPQLLII   MD:Z:86C0   RG:Z:0  BI:Z:QOOOLPMORRPQQKKPQQHOMPPQOQOGNPOPOONNGNOPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOQQROPMM   NM:i:1  AS:i:86 XS:i:19
HWI-ST142_0240:1:5:19100:129082#0   1040    chr3    58125535    60  100M    *   0   0   GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT    988;>ABC??<D?@BBB<A;?8?BB@ACABAB@BB@BCCDAC>@C@?A@@>?BD@?C@B@AB@?C@@?B>BDABDA@7@D=>@>A@>>?BB?>?<;3244    BD:Z:JJLMLCKKNMNNOKAJLLMMMKKAJKNIKAIIKIIKAAIKJMMMLLMJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJJMKAKJNOPOJMII   MD:Z:100    RG:Z:0  BI:Z:JJOPNGNMQQQPQPHOQPQPPOOHNPPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPRRRJOMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:6:11384:77072#0    1040    chr3    58125535    60  100M    *   0   0   GGGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCT    387:=AAB@?BD>@BBB>AA?5=CBA?B?@CD@@D@BBBD@B?@B?=@?@>AAC?=B=B>>B>?C8>?A=?A=?@;=6;@=<==>@9<=>>>><;<6123    BD:Z:JJLMLCKKNMNNOKAJLLMMMKKAJKNIKAIIKIIKAAIKJMMMLLMJKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJJMKAKJNOPOJMII   MD:Z:100    RG:Z:0  BI:Z:JJOPNGNMQQQPQPHOQPQPPOOHNPPLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPRRRJOMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:26:7487:94776#0    0   chr3    58125536    60  100M    *   0   0   TGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    4324<>@>??B??DBBB>B??7ABB@CA@CB?@C?@BBC@AA@DA>@AC@?EB?@?@@@>A@?>A@:1B?@@ABAA<6?@?BE?B@2?ABC@D9@>;9>;    BD:Z:JJMLPNCLKMLNMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLINNLLLNMJMJCLJQOOMJLJ   MD:Z:0G99   RG:Z:0.1    BI:Z:NNOPRPIONQPQQPNHPOOPPMPMHNNPMMHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQQQQQRPLROIPNQPOOKPN   NM:i:1  AS:i:99 XS:i:0
HWI-ST115_0148:1:42:20655:156999#0  1024    chr3    58125536    60  100M    *   0   0   GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    4218;>@>?>B??DBBB;BA?7@BBAC?@CB?@B?@BBB?@@?D@>@@C@ADC@A?A@@?A?>;@A@DB?B@AB@A47A;=>B;?@?>BCC@A>A>;8>>    BD:Z:JJJLPNCLKMLNMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLINNLLLNMJMJCLJQOOMJLJ   MD:Z:100    RG:Z:0.1    BI:Z:NNJPRPIONQPQQPNHPOOPPMPMHNNPMMHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQQQQQRPLROIPNQPOOKPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:6:17640:16154#0    1024    chr3    58125536    60  100M    *   0   0   GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    4226;>@>?>B??DBBB?B??7@BBAC?@BB?@B?@BBB?@@?E@>@ACAAEC@A?AA@?A???AA@DB?BBAB@A@7@@??C?B@??BCC@E??@=:>>    BD:Z:JJJLPNCLKMLNMMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLINNLLLNMJMJCLJQOOMJLJ   MD:Z:100    RG:Z:0.1    BI:Z:NNJPRPIONQPQQPNHPOOPPMPMHNNPMMHONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQQQQQRPLROIPNQPOOKPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:44:21041:193043#0  1024    chr3    58125536    60  100M    *   0   0   GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    4224;>@>?>A??DABB?B??7@BB?C?@BB?@B?@BBB?@@?D@>@@C@>DB?@?@A?>A@>;@?>BB>AAA@@A@7A@9@A?B@>@C=B=A>?=9;9;    BD:Z:IIJKPMDLJMLNNMH@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLNILMMIMKLMLIMMIMLLNMLNMMKLINMLLLMLKMJBMKPPNLJLI       MD:Z:100    RG:Z:0  BI:Z:MMJPRPIOMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPQPQOPNQPQQQQPKQOIPMPQNOJPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:43:6266:2559#0 16  chr3    58125536    60  100M    *   0   0   GGGATGTGTATGCAGATATTCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGATGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    639=@'A==@A;:<*8.=5,4.>@?@B<?CC:79<A@9=<A;@B<<@?@<A>B@?;>A7:=-8@=A>=8@??=A9=45A0:1==>;,9@?85;6020752    BD:Z:JMNMJJKNMNNNNKNLLLKNLJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLMLHHKMIMKKLKMLKMLKHMMMMKLKILJAKJOMOOMNKJJ   MD:Z:5T8A4G41G38    RG:Z:0.1    BI:Z:KOQQKLMQQRQRQPRQQQPQOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQOQQMMPQNQPPQOPQOPQNLONNPOPPJOOHNNPPQQLOPNN   NM:i:4  AS:i:80 XS:i:19
HWI-ST142_0240:1:1:5882:180128#0    1040    chr3    58125536    60  100M    *   0   0   GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    ;8<=@AD@>BD?@BBB?BD?8@BBA@EABBE@BE@BBCDAB??C@@@@@>?BD@?B@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@>B><72464    BD:Z:JLMKBKKNNNNOLAJLLMMMKKAJKNIKAIIKIIKAAIKJMNLLLMKKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJIMKAJJNMPNNMJII   MD:Z:100    RG:Z:0  BI:Z:JOPPENLPQRPQPHOQPQPPOOHOOQLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPRRLOOMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:47:16817:196618#0  1040    chr3    58125536    60  100M    *   0   0   GGGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTT    ;;:=@AD@>BD?@BBB?BD?7@BBA@E@CBE@BE@BBCDAC??C@@@@@>?BD@>C@D@AC@@DA@?B>BDABDA@7@D>>@>B@>>?BB?>B><72464    BD:Z:JLMKBKKNNNNOLAJLLMMMKKAJKNIKAIIKIIKAAIKJMNLLLMKKJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJIMKAJJNMPNNMJII   MD:Z:100    RG:Z:0  BI:Z:JOPPENLPQRPQPHOQPQPPOOHOOQLNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPRRLOOMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:61:20673:123650#0  0   chr3    58125537    60  100M    *   0   0   GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC    4246;>=?=B??DBBB>B??7@BBAC?@BB?@B?@BCB?A@?D@>@ACA@EB@A?B@A?@@>?@A@DB?B@BB@B@7A@>?C<?@<>C9C@D==?<:==5    BD:Z:JJLMNDMKNLNNMIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHNNLLLNMJMJBLJPPONJLJK   MD:Z:100    RG:Z:0.1    BI:Z:NNPPQIPNQPQQPNHPPOPPMPMGONONMGONNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPQQQRPLROIPNRQPMKPNO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:47:16975:197510#0  1024    chr3    58125537    60  100M    *   0   0   GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC    3246;>=>>A??DBBA?B?=8?BA@C??BB??B@@@BB@@@?D?>?@C@@DA?@?.;<39@?>AB@BB?B8A>AA=1;?>>B?B@@@DBA@B9??;9;57    BD:Z:IIKLNDNJMMNNMI@JKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMILMNHMKLMLIMMIMLLNMLNMMKLINMLLLMLJMJBLKPPOMJLIJ   MD:Z:100    RG:Z:0  BI:Z:MMPPPIQMPPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPQPOPNQPQQQQPKQOHPNQQPMJPNO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:66:20946:104000#0  16  chr3    58125537    60  100M    *   0   0   GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC    1979?D=?BC>@B=B?BE?5?ACB@8*6&@@>B9CCCD@B@@C?A@?A>AAE@?C@D@@A@@DA@AB?BEABDA@7@E?>@>@@>>ACB@?B?>:85643    BD:Z:MNLBKJOMNONKAKLLMNNLKAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@KJNMNNMQKKJJ   MD:Z:100    RG:Z:0.1    BI:Z:OQPHLLPQRQRPIPQQRQQOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPPPPPPOLONNPOOPJOOHNNPPPQKQPONN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:8:18877:191709#0   1040    chr3    58125537    60  100M    *   0   0   GGATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC    577-9B>>BC=>:B??;D?2@CCB@C;=A?;A9=@CA9>C??C7@B>B>@BD><C<=;9?2:74<A>?AA>>A<=+<A=:=>AA==:AA?=A>>;:66.3    BD:Z:LMKAJKNNONOLBJLLMMMKKAJKNIKAIIKIIKAAIKJMNMKLMKLJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILKAJINMNNMQJJII   MD:Z:100    RG:Z:0  BI:Z:OPPGLLOPRQQPHOQPQPPOOHOPPMNGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPRLQONMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:28:19470:70891#0   0   chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    4466;;>=A??DBCB>B??7@BBAC?@BB?@B?@BBB?@@@D@>AACA?EB@A>?A?>@@?>@@@DB?B@BBAA@8@@@AC?B@?ADCCAE?AA?>?=;=    BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMNLLLNMJMJBKJPOPNKLJKL   MD:Z:100    RG:Z:0.1    BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNGNNNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPQQRPLROIPNRRQNIPNOP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:42:7431:10467#0    1024    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    4466;;>=A??DBBB>B??7@BBAC?@BB?@B?@BBB??@?D@>A@C@@DC?A?@A@>A??;@@@DB?B>B@AB?7A@>?C?B??ADCB@;8<=;===;=    BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMNLLLNMJMJBKJPOPNKLJKL   MD:Z:100    RG:Z:0.1    BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNGNNNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPQQRPLROIPNRRQNIPNOP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:65:1258:51190#0    1024    chr3    58125538    42  26M74S  *   0   0   GATTTGTATGCAAATATGCGTTTCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN    3558====A@>C<C@;A><4>AB?%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIJJJJJJJJJJJKKKLKKJJJJ   MD:Z:26 RG:Z:0.1    BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNMMNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNOOOOOOOOOOOONMLNNNN   NM:i:0  AS:i:26 XS:i:0
HWI-ST115_0148:1:7:18508:91166#0    1024    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    3558><>=:?>BB@B>A??7@CC@C@9@C@@C@@CCC@A@?D??A@C@?4B??>?A@?????BA5DB?B@BBAA?7??9>C?B@??DCC@D>@>==?=;>    BD:Z:JJMKDNLNMNNNIAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMNLLLNMJMJBKJPOPNKLJKL   MD:Z:100    RG:Z:0.1    BI:Z:NNPOJPOQPQQPNHPPPPPMPMGNOOMNGNNNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPQQRPLROIPNRRQNIPNOP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:67:12142:176815#0  16  chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    9=<5B2:,1125$?;@C:04BC?<D690D:@.9B<CC@C;@A=?A=@<@@@>=B=>7>?:;C=>>B>>A@>@??3<@?:=>;A;=>C@??B??<::8453    BD:Z:NLBJJNNNOOKAKLLMNNLKBJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JJNLNMLQNKNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPHNKOPRQRPIPQQRQQPOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQPOQPNMONNPOOOJOOHNNPPPPKPROQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:7:5473:177428#0    1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    9==>B==0=>@<<B?@B96=CCA?D>CCD@5B?@@@D>A=@@:<<>A?A:A=;94823@)8:;@@<9AA=@@<>6<@5:=?B@=>@CB@>B><<::9423    BD:Z:NLBJJNNNOOKAKLLMNNLKBJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JJNLNMLQNKNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPHNKOPRQRPIPQQRQQPOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQPOQPNMONNPOOOJOOHNNPPPPKPROQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:28:9824:174688#0   1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTAACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    69:=A???B><+@B??@>64B>A?@?AAC>AB=BC>C@A??A>4@:3>ABD@?C@@;;?:8>&>??<>C@A@3?6?B=>>>>@==?AA>?A>@:;96424    BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKKIIHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII   MD:Z:62G37  RG:Z:0  BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOOPMMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:43:5151:42131#0    1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    5<:=B?>BD??BBB>@A=5$BB@@D@CBEACD@CBBD@C??B@@@@@>?BD@>C@D@AB@@D@@?B>BDABDA@7@D?>@?B@>>?BB@?C??<:96434    BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII   MD:Z:100    RG:Z:0  BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:46:16188:21914#0   1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGGTGACGTGCCTATCCCAAACAGTCCCTTCA    9=;=B?>BD??BB??BD?7@BB@@E@ABD?BD@BBBD@C??C>@A@@>?BD@>B@D@AC@@DA@?B>BD@@DA@7@D?>@>B@>>?BB@?A??<:96434    BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLKKGJLKJGLLLLKKJILJAJIMMNLMPNJMII   MD:Z:70A29  RG:Z:0  BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPOLPPONLOOOPOPPJOOGMMPPPPLQQNPMM   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:46:5500:75404#0    1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    99<=B??BD??BCB?BD?7@BB@@E@BBEABD@BBBDBC??B@@A@A>?BD@?C@D@AC@@DA@?B>BDABDA@7@D?>@>A@>>?BB@?C??<:96434    BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII   MD:Z:100    RG:Z:0  BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:46:9470:13130#0    1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    <<==B?>BD??BCB?BD?7=BB@@E@CBEACD@CBBDBC??C@@A@A>?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C??<:96434    BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII   MD:Z:100    RG:Z:0  BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:7:12534:169939#0   1040    chr3    58125538    60  100M    *   0   0   GATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCA    7<<=B??BD??BCB?BD?8@BBB@D?CBDACC@BB@DBB??C@@@@A>?BD@>C@D@AB@?D@@?B?BD@BD@@7@D?>>>B?>>?BB@?A??<:96434    BD:Z:MKAIJNNOOOLBKLLMMMKKAJKNIKAIIKIIKAAIKJMNMLKMKLKLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJAJIMMNLMPNJMII   MD:Z:100    RG:Z:0  BI:Z:PPGNJOOQQRPHOQPQPPOOHOPQLOGNLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPLQQNPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:62:21313:158611#0  0   chr3    58125539    60  100M    *   0   0   ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA    45668<<A>?DBBB?B??7@BBAD?ACB?@B?@BBB?A@?E@>@@CA@EB@A;@AA<B??AAA@D>?B@AB@A?5A=?>B?B>>?D@C?C==,<%%%%%%    BD:Z:JJKANMOMONNJAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMLLLNMJMJBKIPOOOKMJKLJ   MD:Z:100    RG:Z:0.1    BI:Z:NNOHQORPQQPNHPPPQPMPMGNNPMMHNMNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPQRPLROIPNRRROJNNOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:66:2007:42196#0    1024    chr3    58125539    60  100M    *   0   0   ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA    46688<<A>?DBBB>B??7@BBAC?@BB?@B?@BBB?A@?EA>AAC@@DB@@?@@A>A@>?A>@DB=B@@A@>@7@;;:@<=:=9DBB@B>99>7<=753    BD:Z:JJKANMOMONNJAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMLLLNMJMJBKIPOOOKMJKLJ   MD:Z:100    RG:Z:0.1    BI:Z:NNOHQORPQQPNHPPPQPMPMGNNPMMHNMNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPQRPLROIPNRRROJNNOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:8:8758:124039#0    1024    chr3    58125539    60  100M    *   0   0   ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA    35679==A>@ECCB?B?@8@CCAD?@CC?@C?@CCC?AA@EA>AADAADB?@?A@@>AA>?@B@DB?BABB@A=6A@>>C?B@ABDC<@E??@>=A?:?;    BD:Z:JJKANMOMONNJAKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMLLLNMJMJBKIPOOOKMJKLJ   MD:Z:100    RG:Z:0.1    BI:Z:NNOHQORPQQPNHPPPQPMPMGNNPMMHNMNONNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPQRPLROIPNRRROJNNOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:21:16438:172597#0  1024    chr3    58125539    60  100M    *   0   0   ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA    3557:<9@>=B@@==?>=3?@???@@A@>@B>@BBB>A=>B=>?@B@=CA@==??@;?>>:?A>B4:947>@B?5@:9<@3?49=BAA8B%%%%%%%%%%    BD:Z:IIJAOLOMOOMIAKKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKMNIMJLMLIMMIMLLNMLNMMKLINMLLLMLJLIBLJOPONLMIJKI   MD:Z:100    RG:Z:0  BI:Z:MMNGQORPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPOONQPQQQQPKQOHOMRSQOJNNOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:22:4826:29353#0    1024    chr3    58125539    60  100M    *   0   0   ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA    46668<<A>?DBBB>B??7@BB@A?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>B@>:@A?D@?B@AA@A?7@??@B<B@>@BBB@B???>=?=7;9    BD:Z:IIJAOLOMOOMIAKKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKMNIMJLMLIMMIMLLNMLNMMKLINMLLLMLJLIBLJOPONLMIJKI   MD:Z:100    RG:Z:0  BI:Z:MMNGQORPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPOONQPQQQQPKQOHOMRSQOJNNOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:5:4421:176647#0    1024    chr3    58125539    60  100M    *   0   0   ATTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAA    4666:<<A>?DBBB>B??7@BBAC?@BB?@B?@BBB?@@?C@>@@CA@DB?@>@@@>A@>@?A@DB>B@BB@A@7A??@B>B@>@CAC@D@@A>?A=<>9    BD:Z:IIJAOLOMOOMIAKKKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKMNIMJLMLIMMIMLLNMLNMMKLINMLLLMLJLIBLJOPONLMIJKI   MD:Z:100    RG:Z:0  BI:Z:MMNGQORPPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPOONQPQQQQPKQOHOMRSQOJNNOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:22:14446:26100#0   0   chr3    58125540    60  100M    *   0   0   TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGAGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    225:9::<297<<-0=;4;==:9;?9@@77?AB;:>A?<D;<<=@@>DBA%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJAKMPNOONJBKKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMKKJLHLKKMMLMMLKLHMMKLLNMJMJBKIOOONLMKKLJK   MD:Z:59T40  RG:Z:0.1    BI:Z:NNHOPRQQQPNHPPPQQMPMGNNONMGOMMONNHHOQPRPQOPQONOQQQNQQQMOPPOPPOPMQPPQQPQQPOQMQPPPPRPLROIPNRRRPKOLOPNP   NM:i:1  AS:i:95 XS:i:19
HWI-ST142_0240:1:48:10504:20320#0   1024    chr3    58125540    60  100M    *   0   0   TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    3555::@><C?6?7>@>7@AA?A?@BB??B??B@B?@>>A?;?>?@?B?A?:>??<?>>26>4B9::<A=@A?68=-79>7>0:@A7>@488/8??;%%%    BD:Z:IIAKMOOOONIAKLKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLNIMKKMLIMMIMLLNMLNMMKLINMLLLMLJLIALJOOONLNJJKIJ   MD:Z:100    RG:Z:0  BI:Z:MMGOORRPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNPMQPQQQQPKQOHOMQSRPJPLOPNO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:8:20458:106737#0   1024    chr3    58125540    60  100M    *   0   0   TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    46639:@>>DBBB>B??7@BBAC?@BB?@B?@BBB?A@?D@:@ACA>DB?@(??@>B@?>@AACA?B>AA@A@7A?>>C?BA@@DBB@6<@>>>A@<=<;    BD:Z:IIAKMOOOONIAKLKMMJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLNIMKKMLIMMIMLLNMLNMMKLINMLLLMLJLIALJOOONLNJJKIJ   MD:Z:100    RG:Z:0  BI:Z:MMGOORRPPONGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNPMQPQQQQPKQOHOMQSRPJPLOPNO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:2:12658:158403#0   16  chr3    58125540    60  100M    *   0   0   TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    ==@<=BE@@CCC=CE@8ACCAAFACCE@CEACCCEBD@ADAAAAA?@BDA>C@E@AC@@DA@?B>BDABEA@7@D?>@>BA>>?BB@?C@@==>>93674    BD:Z:BJIMMNPOLBKLLMNNLKBKKNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMLMLKOMNQKJJJ   MD:Z:100    RG:Z:0.1    BI:Z:HNMPNPPRPIPQQRQQPPIOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPPPOLOONPOOOINOHNNPPPPJOQPSONNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:47:4453:178967#0   1040    chr3    58125540    60  100M    *   0   0   TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    98=:<>B>?68;==B>26A7A;B?1AD?A?>A?B4<=:>9;>>?B7A@D@;7=C>4?:9>,7))/BA>=C?@38A:6:;-4;9<9?8>A>?<<<>=6553    BD:Z:AIILMOOPMBKMMMMMKKAJKNIKAIIKIIKAAIKJMNMLLMJLKMNLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IIMLMLKNMMQKIII   MD:Z:100    RG:Z:0  BI:Z:GNLOMPOQQIOQPQPPOOHOPQMOGOLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOQPRONMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:67:1442:112789#0   1040    chr3    58125540    60  100M    *   0   0   TTTGNATNNNAANATNCTTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    %%%%%%%%%%%%%%%%%%%%A@B?C<=+92=@5@B:@=AC;:,>91:73,=A:A;3</14.;4=3>799;;=55A:.>=4?=>?AA??A>@<<<>;7553    BD:Z:AIIIJKKKKJJJJIIIJAAJKNIKAIIKIIKAAIKJMNMLLMJLKMNLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IIMLMLKNMMQKIII   MD:Z:4T2G0C0A2T2G1G82   RG:Z:0  BI:Z:GNMMKMMNOONNNNNNPHHOPQMOGOLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOQPRONMM   NM:i:7  AS:i:83 XS:i:19
HWI-ST142_0240:1:68:10518:127054#0  1040    chr3    58125540    60  100M    *   0   0   TTTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAG    =9<;=AD?@A<B?BD?7@B?@@D?BBC>AC@BBBCAC??C@@A@A>@@D?>C@D@@B>@D@A?B??D@=D@@8@D==>?@A>=?BB??C@@==>>93674    BD:Z:AIILMOOPMBKMMMMMKKAJKNIKAIIKIIKAAIKJMNMLLMJLKMNLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IIMLMLKNMMQKIII   MD:Z:100    RG:Z:0  BI:Z:GNLOMPOQQIOQPQPPOOHOPQMOGOLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOQPRONMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:25:1522:126422#0   0   chr3    58125541    60  100M    *   0   0   TTGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGG    46367>=>DCBB?B?>7@CB@C?@AC@A=@@CBC?A@?D@>@@CA@EA?A?@@@?B??;@?@CA7>@@@@@@7?@?BC?B=>?CCC?3@??:>-B%%%%%    BD:Z:JJKJPOPOOJBLKKMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKLNMJMJBKIONONKNKLLJKN   MD:Z:100    RG:Z:0.1    BI:Z:NNONSQRQPNHPPPQQNPMGNNOMNGNNMNNNHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQPLROIPNRRRPLPMMPNPO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:28:2058:137769#0   16  chr3    58125542    60  100M    *   0   0   TGTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGT    >;:=B>@CBB6BD?4@CCB@E@CCE?>D@CACD@C?@A>@?@<>@BE@?C@E?@C>@E@@AB?AE@BE@@7@E?>?>?>>>ABB?A@?===?A>==8243    BD:Z:IMLMOOMBLMLMNNLKBKKOIJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLLJNLLPNMLMJJ   MD:Z:100    RG:Z:0.1    BI:Z:MPPQNPOIPQQRQQPPIPPQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQNLPNNQOOOINNGNNPPPPJOPORPPPONN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:63:2187:107403#0   0   chr3    58125543    60  100M    *   0   0   GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    44268;BAAB>B??7@BBAC?@BB?@C?@BBB?A@?E@=@@AA@EB?A?@?A>B@?8@@@DB=BB@BA@?7A@5?A?B@>>DCC?E@A@??CC@%%%%%%    BD:Z:JJMLQQPKCLLLMMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMMJMJBKIONNMKMKMMKKNMI   MD:Z:100    RG:Z:0.1    BI:Z:NNQPSRQNHPPPQQNQNGNNOMMGOMMOMMHHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKROIPNRRRPLQOOOLPOPM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:45:13340:71390#0   1024    chr3    58125543    60  100M    *   0   0   GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    44268;BAAB>B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@CB?@>@@?>@@>@@A@DB?B?@B?@?7@@?@D>BA@>DBB@D@@@>@BBAC@:867    BD:Z:IILLRQPJBLLLNNJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLKLMLHMMIMLLNMLNMMKLINMLLLMLJLIAKINONMKNKLMJJMLI   MD:Z:100    RG:Z:0  BI:Z:MMPPRRQNGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPPPQQQPKQOHOMQRQPLROOPLOOOM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:6:4941:69627#0 1024    chr3    58125543    60  100M    *   0   0   TTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    2227<76;;:<>@:3?A??A???A??>>?@BB?@@?A@>@@B>>DB@???A@>A?<9==:?=:5::>9>=7A@?9A=6>>0B@<<B<?9;>A@=%%%%%%    BD:Z:IIJLRQPJBLLLNNJKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLKLMLHMMIMLLNMLNMMKLINMLLLMLJLIAKINONMKNKLMJJMLI   MD:Z:0G99   RG:Z:0  BI:Z:MMPPRRQNGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPPPQQQPKQOHOMQRQPLROOPLOOOM   NM:i:1  AS:i:99 XS:i:0
HWI-ST142_0240:1:23:14443:42600#0   16  chr3    58125543    60  100M    *   0   0   GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    ;9<?=>BBB>>D@7@BB@@D@BBDABEACCBDAD??BA@A@@>?BD@>C@D@AC@@D@@@B>BDABDA@7@D?>@?B@>>?BB@?C@@>>@A?=><4484    BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII   MD:Z:100    RG:Z:0  BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:3:18249:30680#0    1040    chr3    58125543    60  100M    *   0   0   GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    99=?=?BBB?BD@7@BB@@>@BBD@CD@BBCC@D??BA@@@@>?BD@>C@D@AC@@D@@?B>BD@BDA@7@D?>@>B@>>?BB@?C@@>>@A?=><4484    BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII   MD:Z:100    RG:Z:0  BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:44:7743:26975#0    1040    chr3    58125543    60  100M    *   0   0   GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    ;9=?=>BBB?BD?7@CB@@D@BBDABEACCCD@D??CA@@@@>?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@A?=><4484    BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII   MD:Z:100    RG:Z:0  BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:7:12525:180227#0   1040    chr3    58125543    60  100M    *   0   0   GTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTG    99=?=@@@@>>D?7=BB@?>@ABD@CD?BBCDAD?@B?@A@B>?BD@?C@C@AB>@D>??B>BD@ADA@7@D>>@>A@>>?BB@?C@@>>@A?=><4484    BD:Z:LLMMOMCLNMNNNKKAJKNIKAIIKIIKAAIKJMNMLLMKLKLNMILKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKMKJMKKNNLPLHII   MD:Z:100    RG:Z:0  BI:Z:OOPONOGORQQPPOOHOPQMOHOMNOLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPQPROLMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:22:11114:21452#0   0   chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    42638@@AA>B??7@BBAC?@BB?@B?@BBB?@A?D@>@ACA@CB?@>@@A>A@>;AB@DB?BAAB@A@5@A>?C?B@?AECCAE?A@?ACC@BC>;;<9    BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIONNMJMKLNKLNMIM   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNRRRPLQOPPMNOPMO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:27:17514:154190#0  1024    chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACACTCCCTTCGAGGGGG    32468@@?>=A?>7A>:>;?@A?@@A@ACCC@A>?C??@@C@?3B?:;;;>9A?<A@A?DB?545:@?0(@A*>B0A=:;-=5(8'93/7CB?%%%%%%%    BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIILKMJMKLNOMNJJJ   MD:Z:85G7A3T2   RG:Z:0.1    BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNNQOPLQOPOPNOJJJ   NM:i:3  AS:i:88 XS:i:0
HWI-ST115_0148:1:42:20622:45748#0   1024    chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    42638@@AA>B??7@CBAC?@BB?@B?@BBB?@@?D@>@ADA@DB?@>A@@>AA??@A@DC?C@ABAA=7A@>?B?B@?AECCAA@A@?ACC@DA>:<;9    BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIONNMJMKLNKLNMIM   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNRRRPLQOPPMNOPMO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:61:15878:77743#0   1024    chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    42638@@BA>B??7@BBAC?@BC?@C?@BBB?A@?E@>@ACA@DB?@?@@@>AA>@AA@DB?CAAC@B@8AA?9A>A<=>DCC@DAAB?AC@@>C>;<;9    BD:Z:JJLNQQLCMLLNMKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLJMJBKIONNMJMKLNKLNMIM   MD:Z:100    RG:Z:0.1    BI:Z:NNPQSQOHPPPQQNQNHNNOMMGNNMNNMGHOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQOIPNRRRPLQOPPMNOPMO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:28:17271:25147#0   1024    chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    42638@@@A>B?>7@BBAC@@BA?@B?@BBB?A@?D@>@ACA@DB?@?@@?>A@>@?A@DB?B@AB@@@8@@?AC?B@@@ABA?B?A@>=@B@CA=99:5    BD:Z:IILNRPLBLMLNNKKI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJLMLILMIMLLNMLNMMKLINMLLLMLJLIAKINNNMKMKLMKKMLIM   MD:Z:100    RG:Z:0  BI:Z:MMPPRQPGPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOQPQQPKQOHOMQRQPKRPPPNMOOMO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:23:15894:66320#0   1040    chr3    58125544    60  11S89M  *   0   0   TCTTCCGATCTTATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAATAGTCC    %:;==5>?B=C?B@5@AC;?@B(79@7A=C?>9D9C7<CB@D?C;AC=@A?;?@=D@?C><?@@?@E9AAB?BE@?@>?4@D<;??B@<:?B<?;<5613    BD:Z:KKKLNMPNLLJLMNNKAKLLMMMKJAJJNHJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKJMKBLNOOMLJJ   MD:Z:83C5   RG:Z:0.1    BI:Z:OPOPMNQQPQOQRQRPIPQPQPPOOHOOQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQOOQOPQOPPNLONOQPPPJOOHOQQRPPNN   NM:i:1  AS:i:84 XS:i:0
HWI-ST115_0148:1:24:10729:139872#0  16  chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    :=>;@CCB?BE?7@BCA?EBCCEBCD@CCCDAC@ACA@A?A?@BFA?CBEA@D?@E?@?B?BE@BEA@8AE@>@?BA>>ACC??B@@=>AC@>AA;5843    BD:Z:LMNNLBMMMNNNLKBKKOIKBIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKINKKOLLOPIMJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQPQMGOQQRQQPPIPPRNOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMONOPOPOINNGMMPPPPJOPOQOOQQMQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:41:8779:7311#0 1040    chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    :=?;>CCB?BE?8@CBA?D?8CE@CDACCCE@D?@D?@AAA?ABFA?D@E?@D?@CB@?B?BD@AD@@7AD?>@?BA>??BB@?CAA>>AC?>A?94823    BD:Z:LMNNLBMMMNNNLKBKKOIKBIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKINKKOLLOPIMJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQPQMGOQQRQQPPIPPRNOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMONOPOPOINNGMMPPPPJOPOQOOQQMQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:42:5182:53868#0    1040    chr3    58125544    60  100M    *   0   0   TATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGG    ;=?;@CBB=BE@8ACDA?F@ABE@BB@ACBE@C@@C?@B>@>A@DA?D@E?@C?@D@@AB>BDABD@@7@D??@?BA>>?CC??B@>>>@B@>A?95844    BD:Z:LMNNLBMMMNNNLKBKKOIKBIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKINKKOLLOPIMJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQPQMGOQQRQQPPIPPRNOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMONOPOPOINNGMMPPPPJOPOQOOQQMQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:1:2492:135734#0    0   chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGCGACATCTGATGACGTGCCAATGACAAACAGTCCATTAAAGGAGGC    3446??@?=@=<6@=@<A?33=<=;==@B8?A>,A?<=:@?=>BAA??A%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJNNQMDMMLNNKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMMKMLHLMJMMLMMLKLHMMLIKMMLIJBKIONNMMMLKKDLOLLNN   MD:Z:54T5A12T2C0C10C2C4T3RG:Z:0.1   BI:Z:NNQQROIPPPQQNQNHONOMMGNMNNMNGGOQPRPQOPQONOQQQNQQQMOPPOONQPMQQOPQPQQPOQMQPQNPQQPMNIPNRRRPRRPQOHOMPPOO   NM:i:8  AS:i:63 XS:i:19
HWI-ST115_0148:1:5:15089:25076#0    1024    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    4533?>@A>B@;8@CC?C?@BC?@B?@BCB?@@=D@=@ACA?EB@@>@@?>A@>A@AAEB?BBAB?A=7@B=?C=A@??DCC@B?A@A?CC@B:@<:;:9    BD:Z:JJNNQMDMMLNNKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLIMJBKIONNMJLKLMLLOMIMN   MD:Z:100    RG:Z:0.1    BI:Z:NNQQROIPPPQQNQNHONOMMGNMNNMNGGOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNIPNRRRPLQOPQNOMPMOO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:61:3907:69136#0    1024    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    4633=>AA=B??7@BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@??AA>A@AAEB?C@ABAA@7AA?AC?BBBADDCAA@A@?ACC@DC?<=:9/    BD:Z:JJNNQMDMMLNNKLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLIMJBKIONNMJLKLMLLOMIMN   MD:Z:100    RG:Z:0.1    BI:Z:NNQQROIPPPQQNQNHONOMMGNMNNMNGGOQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNIPNRRRPLQOPQNOMPMOO   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:45:12621:117199#0  1024    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    3533=>@A>A>?8?BA=A?@A???B??BBB?A??D@>?@@>5A@?A?@?A?A=?6<0>?@:<7><@A;7@<;>B>?:8>A:C=%%%%%%%%%%%%%%%%%    BD:Z:IINNQLDLMMNNKLI@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKMLIMLIMLLNMLNMMKLINMLLLMLJLIAKINNMMKMJLMKLNLIMM   MD:Z:100    RG:Z:0  BI:Z:MMPPQPIPOPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPQPQPKQOHOMQRQPKQPQQNOMOMOO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:22:9598:56018#0    16  chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    >@:;BCB?CE@8@CCA@EACBEACEACCCEBD@@DAAAAA?@BEA?DADAAC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@>AA<98434    BD:Z:MNNKBLNMNONLKBKKOIKBJHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMKKNLKNPLMNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPQOFNPQRQQPPIPPRNPHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPNNQOOPINNGMMOPPPJOPOQONQPOQPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:47:10637:156404#0  1040    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    <?,<BAB?@D=6?@BAADACCD@BD@CCCDBD@@DA@A?B>@BEA>CADA@C?@D@AAB?BEABD@A5@D?>@?BA>>?CC@?D?A>>AB@>AB<98433    BD:Z:MNNKBLNMNONLKBKKOIKBJHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMKKNLKNPLMNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPQOFNPQRQQPPIPPRNPHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPNNQOOPINNGMMOPPPJOPOQONQPOQPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:64:7317:137760#0   1040    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    <@9<AC??BE?4@CCA@E@CCD@>C?BBBD?B?@C?@A@@>A?E@?C@D?@A?8D@@@B?BE@BD@A7@C?>@?B@>?ACC@?B???>>A?>A>;::233    BD:Z:MNNKBLNMNONLKBKKOIKBJHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMKKNLKNPLMNJJ   MD:Z:100    RG:Z:0.1    BI:Z:QPQOFNPQRQQPPIPPRNPHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPNNQOOPINNGMMOPPPJOPOQONQPOQPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:22:7690:52349#0    1040    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    =?::@AB=@B?7ABBA@D@BBD@BE@CBBD@B?@B@?@@A>?BD@?C@D@AC@@D@@?B>BDAADA@7@D?>@>B@>>?BB??C@@>>@B@>AA<98434    BD:Z:MMNKBLNNONNLLAJKNIKAIIKIIKAAIKJMNMLLMKLKMNLILLLGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJJMJJNLJOOLLMII   MD:Z:100    RG:Z:0  BI:Z:POPOENPPRQPOOHOPQMOHOMOOLOGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONRQNPOMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:44:17314:24318#0   1040    chr3    58125545    60  100M    *   0   0   ATGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGC    ;<9;@A=>BD?7?BB@@D@BBD@BE@CCBEBC?@B>@A@A>ABD@>B@D@@C@@D@@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@>AA<98434    BD:Z:MMNKBLNNONNLLAJKNIKAIIKIIKAAIKJMNMLLMKLKMNLILLLGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJJMJJNLJOOLLMII   MD:Z:100    RG:Z:0  BI:Z:POPOENPPRQPOOHOPQMOHOMOOLOGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONRQNPOMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:21:14936:171679#0  0   chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    4338=>@=A??7@BBAC?@BB?AB?@BBB?@@?D@>@ACA@DB?@>@@?>AB>9?AAEB?B?AB@A@7@@@AB?BBAAECD<E>A@B?DC@D@?>@<9:<    BD:Z:JJNNMENMMNNLLJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILJBKIONNMJLJLMKMONIMNN   MD:Z:100    RG:Z:0.1    BI:Z:NNQPPIQPPQQNQNHOOOMMGNMMOMMHGNQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHPNRRRPLQOPQOPNNMOOO   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:4:21260:196166#0   1024    chr3    58125546    60  100M    *   0   0   TGCAAATATGTGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCACACAGTCCCTTCAAGGTGGCT    3239>@@<A?@?@CCAD?ACC?@C?@CCC?AA?CA>@@DA?DB@@?A@?>A@>=A@?DB?C@AB@@@5@@??C=B@?BE@EBE?A8?>3C?B??%%%%%%    BD:Z:JJNNMENMMNJIIJ@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIIIIONNMJLJLMKMONIMNN   MD:Z:10C68A20   RG:Z:0.1    BI:Z:NNQPPIQPPQNMNNHOOOMMGNMMOMMHGNQPRPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQMMNNRRRPLQOPQOPNNMOOO   NM:i:2  AS:i:90 XS:i:20
HWI-ST142_0240:1:7:9321:123796#0    1024    chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    4338;>@=A?>7@BB@C?@BB@@A>?BBB?@@?D@>@ACA@DB?@>@@?>A@>A?@@DB?B?AB?A@7@?@AB?BB@@BBB@D?@A@ACB@D@@=>:97<    BD:Z:IINMMDNMMONKLJ@IILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLLIMMHMLLNMLNMMKLINMLLLMLJLIAKINNMLKMJKMKLOMIMMM   MD:Z:100    RG:Z:0  BI:Z:MMPOPIROPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPQPPKQOHOMQRQPKQOQROOOMMOON   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:1:13411:166721#0   16  chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    >::>AB?BF?68CCB?E@CCD@BD@CCBD@C?<C??B???ABDA?D@D?AC?3E@@@B?BD@BDAA5@D8?@>B@>>?BB@?C@@>>AC@?AB?<?4344    BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:4:12668:186019#0   1040    chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    @:<?AB?BE@7ACCAAEACCEACEACCCDBC?ADAAAAA>ABEA?CAE@BC@@D@@?B?BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?AB><=4344    BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:5:14308:33877#0    1040    chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    <::?AA?BE@7ACCAAEACCEACEACCCE@C?@C?@A?A>@BDA?DAE@BC@@DA@?B>BDABDA@7@D@>@>B@>>?CB@?B??>>@B??BC><=4344    BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:5:7280:194860#0    1040    chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    @:<?AB>BE@6@CDAAEACCD@CD@CCCD@C?@A?@A@@>@BE@?A@E?@C@@DAA?B>BD@BC@A7@E?>@?B@>>?CB@?B@@>>@B@?AB><?4244    BD:Z:NNKALMNNOOLKBKKOIKBJIJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJKNKKMOLPNOJJ   MD:Z:100    RG:Z:0.1    BI:Z:PQOHMOPRQQPPIPPRNPINMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPONPPOOJNNGMMOOPPJOPOQONPPNSPRNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:27:20013:7395#0    1040    chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    ?::=@A?@D?7@CC@@D@BBD@BDABBBD@D??D@@@A@>?BD@>C@D@AB@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?AB><=4344    BD:Z:MNKAKNNOONLLBJKNIKAIIKIIKAAIKJMNMLLMKLKMNMHLLLHKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJIMJJMLJMOKPMOII   MD:Z:100    RG:Z:0  BI:Z:OPOGLPOQQQOOHOPQMOHOMOOMNHGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPQNROQMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:63:14584:29777#0   1040    chr3    58125546    60  100M    *   0   0   TGCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCT    =::=@A>BD?7@CB@@D@BBD@BDABCBEBD??D@@AA@>?BD@?B@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C?@>>@B@?AB><=4334    BD:Z:MNKAKNNOONLLBJKNIKAIIKIIKAAIKJMNMLLMKLKMNMHLLLHKKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJIMJJMLJMOKPMOII   MD:Z:100    RG:Z:0  BI:Z:OPOGLPOQQQOOHOPQMOHOMOOMNHGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPQNROQMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:27:2568:76725#0    0   chr3    58125547    60  100M    *   0   0   GCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTG    4386;><A>?7@BBAC?@AB?@B?@BBB?A@?D>:@@C@@DB?@>@@@2@>>8?@@B?=@@AA=A@5@@?>D;B=>>BBB>D<?>=>%%%%%%%%%%%%%    BD:Z:IIMIENOMOOKLJAIILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKIMMILLLNMLNMMKLINMLLLMLJLIAKINNMLJMJKLKLONJMMMM   MD:Z:100    RG:Z:0  BI:Z:MMONIRQPPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPQOKQOHOMQRQPKQOPRPPOOKOONP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:27:6383:156593#0   16  chr3    58125547    60  100M    *   0   0   GCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTG    %%%=/>@A<4?CCA@B<>-D=.A?B@CC=C?@C?@A=@=@BD@?C@B>??;;A5>@@<>>??C?A//;3';:>?>><CB>@B1@<>?A??A>:=@:6454    BD:Z:NKAKMMOOOMKBKKOIKBJIKIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJNKJMNKPQOOJJ   MD:Z:100    RG:Z:0.1    BI:Z:QOHONOQQQPPIPPRNPIOMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOPOPOIONGMMOOOPJOPOQONPONRRRQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:28:2897:141354#0   1040    chr3    58125547    60  100M    *   0   0   TCAAATATGCGTTTCTGCTTATTGTTTTGAGCAGGGCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTACCTTCAAGGTGGCTG    %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%C:@A@;AC5.:.=327')16913(@BC@93)229=>>=:856:33752    BD:Z:NKAKMMOOOMKBKKONOKJLLIHJAAIKJMMMKIMNKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLLLMJJNKJMNKPQOOJJ   MD:Z:0G16T2G14T48C15    RG:Z:0.1    BI:Z:QOHONOQQQPPIPPRQSQOPPNMOHHNPNOQPPKPRPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOPOPOIONGMMOOOQPOPOQONPONRRRQNN   NM:i:5  AS:i:79 XS:i:19
HWI-ST142_0240:1:67:5306:137734#0   1040    chr3    58125547    60  100M    *   0   0   GCAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTG    ::==@>BD?7@BC@@D@BBD@BD@CBCEBD@?CA@A?A>?BD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?BB?>@93484    BD:Z:NKAJMNOOOLLBKKNIKAIIKIIKAAIKJMNMLLMKLKMNMIKLLHLKIKKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJILJJMKJMMKOQONII   MD:Z:100    RG:Z:0  BI:Z:POGNNOPPQPOHOPQMOHOMOOMOGHNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPONRQQPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:8:1653:130740#0    1024    chr3    58125548    37  27M73S  *   0   0   CAAATATGCGTTTCTGTTTGTTGTTTTTGAAAGGTGTCCCTAAAATAACGAGGACACAAAACAGCGAGCCCATACCAATGATAGCTAACACAGCGACTGC    %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%    BD:Z:JJJBOONOOLMK@JJMIJ@JIJJIJ@@@JMJAJMLHIMLIKKIAAKKIJKMKMKLHHHIAAJHNNKMKNMILLKLLLIKNNMLNONLJKIJJPQMOMLNN   MD:Z:27 RG:Z:0.1    BI:Z:NNNHRQQQQNQNHOOPNMGNMMNMNGGHNPOHPOPMNQOKPPNHHPPNOOQPOPPMMMNHHOMQRNQPRPKQQPPOQNPQQQQRSPQOPNNNRRMOPPPQ   NM:i:0  AS:i:27 XS:i:20
HWI-ST115_0148:1:8:2670:193217#0    0   chr3    58125548    60  100M    *   0   0   CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT    4866;:@>>7@BBAC?@BB?@B?@BBB?@??E@>AACA@EB?@>@A?>A@>A@A@@?=BBAB@@@7AB??B=B>?BDCC@EA@?A?@C@DB??@@=;=;<    BD:Z:JJJBOONOOLMK@JJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAKIONNMJLJKLKLOOJNNNNJ   MD:Z:100    RG:Z:0.1    BI:Z:NNNHRQQQQNQNHOOPNMGNMMNMNGGOPORPQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMRRRPLQOPQOQPPLMOOPN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:22:15967:185854#0  1024    chr3    58125548    60  100M    *   0   0   CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT    4756;:@>>7@BBAC?@BB??B??BBB?A@?D@>@ACA@DB?@>@@?>A@>=?A@DB?B?@BA@@7AA?>B?B@>ADBB@D?AA?@BC@@B?>@?<:;97    BD:Z:IIIAOOOOOLLJAJILHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKHMMIMKLNMLNMMKLINMLLLMLJLIAKINNMLJLJKLJLONKNMMMI   MD:Z:100    RG:Z:0  BI:Z:MMNGRQRPPMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPPPJQOHOMQRQPKQOPQPQPOMMONPM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:26:7294:74712#0    16  chr3    58125548    60  100M    *   0   0   CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT    ;==>>BD@9ACCAAEACBEACEACCCDBD@@CAAAAA?@CEA?DAEABCA@DA@AB>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?BC??B<84844    BD:Z:KAKLMNPOMLBKKOIKBJIKJHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMKJLNJOQROIJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHOPNPPQPPIPPRNPIONONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQOOPINOGMMOOOOJOPOQONPOMRQTQMNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:8:17018:130894#0   1040    chr3    58125548    60  100M    *   0   0   CAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGT    ;==>=BE>6ACCABEBC@F@CEACCCDBC@@C?AB@A>@BE@?C@D=?=>8C<:@0?BDABD@@7AE?>?=BA>>?CB@?C@@=>?C@?BC??B<85844    BD:Z:KAKLMNPOMLBKKOIKBJIKJHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMKJLNJOQROIJJ   MD:Z:100    RG:Z:0.1    BI:Z:OHOPNPPQPPIPPRNPIONONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQOOPINOGMMOOOOJOPOQONPOMRQTQMNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:27:17632:7595#0    16  chr3    58125549    60  100M    *   0   0   AAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTC    :=;;?E@7ACCA?D=@CD=>E@CCCEBB@AC?@A>A>@BEA?C@EA@D@@D@AAB>BDABDAA7@D?>?>BA>>?BCA?C@A>>@B@?BC@?C>;98444    BD:Z:AKLLNOPMLCKKOIKBJIKJIJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJJLMJNPRRIMJJ   MD:Z:100    RG:Z:0.1    BI:Z:HOPPOOPPPIPPRNPIONPNMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPOOJNNHMMOOOOIOPOQONPOMQQSSMPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:44:19454:138733#0  1040    chr3    58125549    60  100M    *   0   0   AAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTC    ;=<7@D?7@CCAAEACCEACEACCCE@C@ACAAA@A>ABEA?BAEABC>@EAA?B>BEABEAA7@D?>@?BA>>?BB@?C@@>>@B@?CC@?C>;98444    BD:Z:AKLLNOPMLCKKOIKBJIKJIJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJJLMJNPRRIMJJ   MD:Z:100    RG:Z:0.1    BI:Z:HOPPOOPPPIPPRNPIONPNMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPOOJNNHMMOOOOIOPOQONPOMQQSSMPNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:8:1814:174888#0    1040    chr3    58125549    60  7S93M   *   0   0   CCGATCTAAATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGT    -39>?=?CDB?2E?8@CCA>D=6CB@@C@?CCDBD@AB?BB>A>@BE@>C@C?@B?>D@>AA?=DABAA@8@>?>??BA>>ABC@?C?@>>?A><;8433    BD:Z:MLNMLOLBLMLMNNLKBKKOIJAIHJIHJAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLLJNLLPNMLMJJ   MD:Z:93 RG:Z:0.1    BI:Z:OOQPMPPIPQQRQQPPIPPQMOHNMONMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQNLPNNQOOOINNGNNPPPPJOPORPPPONN   NM:i:0  AS:i:93 XS:i:0
HWI-ST115_0148:1:1:20930:85007#0    0   chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    4662;;>6?BBAC?@BC?@B?@BBB?A@?E@>@ACA@DB?@>@@?>A@>@?@@EB?BAAB@B@6@A>?C?B@?>CCC@E@A@??CC@DC@?;=?=A>;9=    BD:Z:JJLLOQPMNKAKJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHONNMJLJKLJKONJOOONJNL   MD:Z:100    RG:Z:0.1    BI:Z:NNPPRRRNQNHOOPNNHNMMNMMGHNPPQOQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQRPLQOPQOQPQNONMPNQP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:26:13065:57592#0   1024    chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    4662;;=6?BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>A@>@@AAEC?C@AC@A@8AA@AD?C@AAECCAEAABAADCBBC@>>B>?A<;;6    BD:Z:JJLLOQPMNKAKJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHONNMJLJKLJKONJOOONJNL   MD:Z:100    RG:Z:0.1    BI:Z:NNPPRRRNQNHOOPNNHNMMNMMGHNPPQOQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQRPLQOPQOQPQNONMPNQP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:44:16873:9543#0    1024    chr3    58125550    60  89M11S  *   0   0   TATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAAGATCGGAAGA    3354<;=6?CCAB@ACB?ABA?CCB@??=EA>@@CA@DA?@>@?@=@@=;@A>BA?BA@B@A?5>A?>>?B???BCC@D?AB??CC@DCCABB>7=><:;    BD:Z:JJLLOQPMNKAKJMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHONNMJLJKLJBLLNPMNLKKK   MD:Z:0A88   RG:Z:0.1    BI:Z:NNPPRRRNQNHOOPNNHNMMNMMGHNPPQOQOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQRPLQOPQOIQPQQNMPOPO   NM:i:1  AS:i:88 XS:i:0
HWI-ST142_0240:1:66:6615:16862#0    1024    chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    4662;;=6?BBAC?@BB?@B?@BBB?@@?D@>@ACA@DB?@>@@?>@@>A?@@DB>B?@B@@A7@??@C?CBAADCB@B@@A@@CBAEB@>@@>>A;98=    BD:Z:IIKLPQQLMKAJJMHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKHLLIMLLMMLNMMKLINMLLLMLJLIAKINNMLJLIJLJKNNKOONMIMK   MD:Z:100    RG:Z:0  BI:Z:MMPORRRMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPPOJQNHOMQRQPKQOPQOPQQNOOLPMPP   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:8:13721:15726#0    1024    chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    4662;;=6?BBAC?@BB?@B?@BBB?@@?D@>@AC@@DB?@?@@?>A@>=?A@DB>B?@B@A?7@@>@B?B@AADCB@C@=A@@BB@E===@@>>?;9::    BD:Z:IIKLPQQLMKAJJMHI@JHIJHI@@JLKNLMLKLIIKKLMHKLMHLJKLKHLLIMLLMMLNMMKLINMLLLMLJLIAKINNMLJLIJLJKNNKOONMIMK   MD:Z:100    RG:Z:0  BI:Z:MMPORRRMOMGONPMMGOMMOMMGGOPOQOPOOPNNOPPPMPPQMOPOOOMPPLPOPPPPPPPNOMPOPPPPOJQNHOMQRQPKQOPQOPQQNOOLPMPP   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:43:4061:135226#0   16  chr3    58125550    60  100M    *   0   0   AATATGCGTCTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    ;<9>;?6?A?AADACCBACDACCCE@C?@CA@A?B?ACC@?CAEA@D?@EA@AB>BD@BDAA7@D?>?>B@>>?BB@@C@@>>@B@?BC@AC?=>=4434    BD:Z:KLLMOONNLMKOIKBJIKJIKAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJILMINOQRLMNJJ   MD:Z:9T90   RG:Z:0.1    BI:Z:OPPQNOOQPQPRNPIONPOMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPPOIONGNMOOOOINPOQONPOMQPSROPQNN   NM:i:1  AS:i:95 XS:i:0
HWI-ST115_0148:1:64:5894:89026#0    1040    chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    =;8>B>7@CCA?EACCEACEACCCEBD?@CAAAAA>ABEA?CAE?BDA@E@@AB>BEABEA@7@E?>@>B@>>?BB@?C@@>>@BA?BC@@C?=<=4434    BD:Z:KLLMOONLCLKOIKBJIKJIKAAIKJMMMKLLJKKLMLHLKKHLLJKLHKMIMKKLKMLKMLKHMMMMKLKILJ@JIMKLKIMJJMJILMINOQRLMNJJ   MD:Z:100    RG:Z:0.1    BI:Z:OPPQNOOPIPPRNPIONPOMOHHNPNOQPPOPOPPOQQMPPPMQONPOMPQNQPPQPQQPQQOMPOOQPPOIONGNMOOOOINPOQONPOMQPSROPQNN   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:3:12949:146598#0   1040    chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    9<8<B>7?BA@@E@ABD@C7@BBBD@B?@B>?BAB=@BC?>C@D@AC=@D?@?B>AD@BDA@7@C?>??B@>>?BB@?A@@=>@B@?BC@@C?=<=5434    BD:Z:JLLMNOMMCKLOJKAIIKIIKAAIKJMNMLLMKLKMNMILLLGLLJLKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJILIILKILLIMNRQMMMII   MD:Z:100    RG:Z:0  BI:Z:NPOPMONOIPPQMOHOMOOMOHHOPONPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPOLPOSRNPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:63:20198:59364#0   1040    chr3    58125550    60  13S87M  *   0   0   GCTCTTCCGATCTAATATGCGTTTCTGCTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTC    58;:?@=6<6A@?BB?@D>2@CBA@D?@@D?AC?BBC=@C?AC@@A@B>?BD@?B@D@@B@@D?@?B>BD@BD@?7?C==@?>@=>?BB@>B?>:74634    BD:Z:OLKJKMNMOMLNKJLLMMMKKAJKNMOJIIKIIKAAIKJMNMKLMKLJLMLHKKKGKKIKKGJLHMKKLJLLJLKJGLLLLKKJILKAJINMNNMQJJII   MD:Z:14T72  RG:Z:0  BI:Z:QOOOLPMORRPQQOQPQPPOOHOPPPQONLNNLNGGNPNNPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPRLQONMM   NM:i:1  AS:i:82 XS:i:19
HWI-ST142_0240:1:6:5523:114409#0    1040    chr3    58125550    60  100M    *   0   0   AATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCA    ==9=B>7@BBA@D@BAD@B>@BBBEAD@@BA@AA@>?AD@>C@D@AC@@DA@?B>BDABDA@7@D?>@>B@>>?BB@?C@@>>@B@?BC@@C?=<=4434    BD:Z:JLLMNOMMCKLOJKAIIKIIKAAIKJMNMLLMKLKMNMILLLGLLJLKGJLHMKKLJLLJLKJGLLLLKKJILJ@IHLKLJILIILKILLIMNRQMMMII   MD:Z:100    RG:Z:0  BI:Z:NPOPMONOIPPQMOHOMOOMOHHOPONPPPOPOOPOPPLOPPLPONPOLPPMPPOPPPPPPONLOOOPOPPJOOGMMPPPPJOONPONPOLPOSRNPPMM   NM:i:0  AS:i:100    XS:i:0
HWI-ST115_0148:1:28:10415:169781#0  0   chr3    58125551    60  100M    *   0   0   ATATGCGTTTCTGTTTGTTGTTTTGAGCAGGTCTCCATGTAGTGGAGGTGACATATGATGACGTGCCTATCCCAAACAGTCCCTTCAAGGTGGCTGTCAC    46268=6?ABAC?@BB?@C@@CCC?A??D@>@ACA@DB?@>@@@?A<?@@>BEB?C@ACAAA6A@@AD?BB?ADCCAC?A@BADCADCA>A@>?5>=:?8    BD:Z:JJLLQQNNLAKKMIJ@JIJJIJ@@JMKNMNMLMJJLLLMILMMHLKKMLHMLHLKKMMLMMLKLHMMKKKMLILIAJHNNNMJLJKLJKNNJNPOOJNLI   MD:Z:100    RG:Z:0.1    BI:Z:NNPPSROQNHOOPNNHOMMNMMGGOPOROPOPQONOQQQNQQQMOPPOPMQPMQPPQQPQQPOQMQPPPPQOKQNHOMQQQPLQOPQOQPQNPONNNQPM   NM:i:0  AS:i:100    XS:i:0
HWI-ST142_0240:1:26:12059:67588#0   1024    chr3    58125551    60  79M21S  *   0   0   ......
ShixiangWang commented 2 years ago

I checked the paired tumor. It seems the issue is caused by the SAM flag. I am a newbie to this. Is it possible to keep them all without considering SAM flag?

$ alleleCounter -l test.loc -b ~/data/gdc/wes/TCGA-AA-3869-01A-01W-0995-10_hg19_Illumina_gdc_realn.bam -o test.txt
[W::hts_idx_load2] The index file is older than the data file: /public/home/zhaoqi/data/gdc/wes/TCGA-AA-3869-01A-01W-0995-10_hg19_Illumina_gdc_realn.bai
Reading locis
Done reading locis

$ cat test.txt 
#CHR    POS Count_A Count_C Count_G Count_T Good_depth
chr3    58125556    0   385 0   0   385
chr3    58125557    0   0   353 1   354
chr3    58125558    0   1   0   401 402
chr3    58125559    0   0   0   405 405
chr3    58125560    0   0   0   398 398
keiranmraine commented 2 years ago

alleleCount is intended to be used on primary mappings of paired-end sequencing. The example you provided has all reads indicated as unpaired (no flag bit 1) and many are PCR/optical duplicates which you would not want to count (useful tool).

The tool could be extended to support unpaired data, but it will be at the discretion of our internal priorities.

ShixiangWang commented 2 years ago

@keiranmraine Thanks for your response. It's strange that this is a normal sample bam downloaded from GDC portal. This indicates the the sample quality is bad and should be not used in my analysis.

It would be nice if the program can print a message when no paired reads found or summarize how many reads filtered out so the result is more readable in such cases.

Thanks again. And I have no questions further.

Shixiang

a3schiller commented 7 months ago

Hi @ShixiangWang and @keiranmraine

I have the same problem (only zeros in my allele count output). I am using bam files from paired-end sequencing. I am using version 4.3.0 of alleleCounter. I found the problem while running Battenberg, but the output is the same even when I am using alleleCounter by its self. 

#CHR    POS     Count_A Count_C Count_G Count_T Good_depth
1       16103   0       0       0       0       0
1       51479   0       0       0       0       0
1       51898   0       0       0       0       0
1       51928   0       0       0       0       0
1       54490   0       0       0       0       0
1       54708   0       0       0       0       0
1       54716   0       0       0       0       0
1       54753   0       0       0       0       0
1       55299   0       0       0       0       0

Thankful for any help on how to solve this!

keiranmraine commented 7 months ago

@a3schiller I am no longer involved in the support of this tool. @AndyMenzies may be able to delegate someone to help.

a3schiller commented 7 months ago

Thakns for your fast reply and delegating the question!

@AndyMenzies, I am thankful for any help on how to solve this!

davidrajones commented 7 months ago

@a3schiller I too am no longer on this project, however, a few useful things to provide would be:

a3schiller commented 7 months ago

An update if anyone is having the same problem in the future.

The problem was that I used an loci-file without chr-prefix but an BAM-file with chr-index. Using an loci-file with chr-index made it work for me!

AndyMenzies commented 7 months ago

Thanks for the update. I've fallen for that GRCh37 -> GRCh38 trap before. Glad you got it working.