Open imendes93 opened 2 years ago
Greetings,
I'm attempting to run ascatNgs and I'm running into the following error:
HREAD_EXITED: General output can be found in this file, last 10 lines below: /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/logs/Sanger_CGP_Ascat_Implement_ascat.0.out STDOUT: [1] Reading Tumor LogR data... STDOUT: [1] Reading Tumor BAF data... STDOUT: [1] Reading Germline LogR data... STDOUT: [1] Reading Germline BAF data... STDOUT: [1] Registering SNP locations... STDOUT: [1] Splitting genome in distinct chunks... STDOUT: [1] Sample pb_sample (1/1) THREAD_EXITED: Errors can be found in this file, output below: /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/logs/Sanger_CGP_Ascat_Implement_ascat.0.err STDERR: + cut -f 1-3 /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/SnpGcCorrections.tsv STDERR: + cd /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/ascat STDERR: + /usr/bin/Rscript /opt/wtsi-cgp/lib/perl5/auto/share/module/Sanger-CGP-Ascat-Implement/ascat/runASCAT.R /opt/wtsi-cgp/lib/perl5/auto/share/module/Sanger-CGP-Ascat-Implement/ascat /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/SnpPositions.tsv /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/SnpGcCorrections.tsv pb_sample pb_sample.count pb_sample pb_sample.count XY 24 /tmp/nxf-3672569323515647077/outdir/ascat/tmpAscat/ascat/pb_sample.Rdata 'c('\''chr1'\'','\''chr10'\'','\''chr11'\'','\''chr12'\'','\''chr13'\'','\''chr14'\'','\''chr15'\'','\''chr16'\'','\''chr17'\'','\''chr18'\'','\''chr19'\'','\''chr2'\'','\''chr20'\'','\''chr21'\'','\''chr22'\'','\''chr3'\'','\''chr4'\'','\''chr5'\'','\''chr6'\'','\''chr7'\'','\''chr8'\'','\''chr9'\'','\''chrX'\'','\''chrY'\'')' 'c('\''1'\'','\''10'\'','\''11'\'','\''12'\'','\''13'\'','\''14'\'','\''15'\'','\''16'\'','\''17'\'','\''18'\'','\''19'\'','\''2'\'','\''20'\'','\''21'\'','\''22'\'','\''3'\'','\''4'\'','\''5'\'','\''6'\'','\''7'\'','\''8'\'','\''9'\'','\''X'\'','\''Y'\'')' STDERR: Error in apply(corr_tot, 1, function(x) sum(abs(x * length_tot))/sum(length_tot)) : STDERR: dim(X) must have a positive length STDERR: Calls: ascat.GCcorrect -> apply STDERR: Execution halted STDERR: Command exited with non-zero status 1 STDERR: 157.74user 2.04system 2:39.79elapsed 99%CPU (0avgtext+0avgdata 2620972maxresident)k STDERR: 0inputs+437232outputs (0major+773683minor)pagefaults 0swaps
Here's the command that i'm using:
ascat.pl \ -outdir outdir/ascat \ -tumour pb_tumor.bam \ -normal pb_normal.bam \ -snp_gc SnpGcCorrections.tsv\ -reference Homo_sapiens_assembly38.fasta \ -minbasequal 20 \ -gender XY \ -gc chrY \ -species human \ -assembly GRCh38 \ -pr WGS \ -pl ILLUMINA \ -c 4 \ -force
In the container quay.io/wtsicgp/ascatngs:4.5.0. The index for the bam files and reference are in the directory.
quay.io/wtsicgp/ascatngs:4.5.0
The input files are publicly available at s3://eu-west-1-example-data/nihr/testdata Thank you for your assistance!
Highly likely this is the same issue as: https://github.com/cancerit/cgpPindel/issues/100
Greetings,
I'm attempting to run ascatNgs and I'm running into the following error:
Here's the command that i'm using:
In the container
quay.io/wtsicgp/ascatngs:4.5.0
. The index for the bam files and reference are in the directory.The input files are publicly available at s3://eu-west-1-example-data/nihr/testdata Thank you for your assistance!