Closed benjjneb closed 7 years ago
Thanks for raising this issue, @benjjneb . I got some advice from @wasade , who has processed mock-3 with qiime1 more recently (I have been using qiime2 without issue on this dataset). He notes that:
it looks like we needed to rc both mapping and the index read
and specify --phred_offset 33
it looks like we also needed to use the simplified headers, where the " 1:N:0:1" bits were removed
@benjjneb if you can confirm that this works for you, and provide a working qiime1 command to me, I will correct this in the mock-3 README and close this issue. Thanks!
Thanks, can confirm the following worked:
split_libraries_fastq.py -i mock-forward-read.fastq -m sample-metadata.tsv -o out --store_demultiplexed_fastq -b mock-index-read.fastq --rev_comp_mapping_barcodes --rev_comp_barcode --phred_offset 33
Thanks @benjjneb and @wasade for testing this! The corrected readme is fixed with #62 .
I am trying to use QIIME 1.9.1 to demultiplex mock-3. When I enter the command listed in the readme (
split_libraries_fastq.py -i mock-forward-read.fastq.gz -o split_libraries -m sample-metadata.tsv -b mock-index-read.fastq.gz
) I get the following error:When I muck around with the revcomp options, I can get past that, but get stuck here:
I've tried specifying phred_offsets of 33 and 64, but that doesn't help.