Closed chunyuma closed 3 years ago
Hi @chunyuma ,
I believe QIIME 1 is still installable as a virtualbox — otherwise the QIIME 2 action qiime demux emp-single
would be the equivalent command. You can see the QIIME 2 documentation for usage instructions, and please see the QIIME 2 forum if you run into issues using that command. Eventually I plan to update the usage instructions for mockrobiota to use QIIME 2 — but in theory any demultiplexing method should work.
There is an example on the QIIME 2 forum of how to use mockrobiota data in an example analysis, but it uses mock-25, which does not need to be demultiplexed — nevertheless, this might help: https://forum.qiime2.org/t/fungal-its-analysis-tutorial/7351
Good luck!
Thanks @nbokulich!
Hello,
I want to use Mock-10 data which has three mock communities. However, after I downloaded the fastq data based on the link provided in dataset-metadata.tsv, I don't know how to identify which mock community a read sequence belongs to. Perhaps I might run the following python command line provided in the README.md:
However, this script might come from QIIME1 but now QIME has version2 and the version2 QIME seems not to provide this script. Also, I tried to run this command line by the script provided here but it doesn't work.
Could you please give me some instructions about how I can know which mock community a read belongs to?
Thanks you!