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carolzhou / multiPhATE

Throughput PhATE processing of draft or finished phage genomes
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Error while configuring annotation #17

Closed outbig closed 4 years ago

outbig commented 4 years ago

Dear Carol,

I am installing the multiPhATE on my workstation step by step. Mutiple gene callers were sucessfully installed and working perfectly. But, I have gotton a error mesage saying below while configuring annotation. I have concatenated the viral.1.protein.faa, viral.2.protein.faa, and viral.3.protein.faa to viral.protein.faa and specified the file path in the multiPhATE.config. Please let me know how to address it. Thanks in advance. Looking forward to hearing from you asap

Best, Yongkyu

BLAST Database error: No alias or index file found for protein database [/home/ykim/software/multiPhATE-master/Databases/NCBI/Virus_Protein/viral.protein.faa] in search path [/home/ykim/software/multiPhATE-master::]

carolzhou commented 4 years ago

My first thought would be that you need to run makeblastdb to format the database for blast. Please try that and let me know if it solves the problem.

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On Mar 23, 2020, at 2:14 AM, Yongkyu Kim notifications@github.com wrote:



Dear Carol,

I am installing the multiPhATE on my workstation step by step. Mutiple gene callers were sucessfully installed and working perfectly. But, I have gotton a error mesage saying below while configuring annotation. I have concatenated the viral.1.protein.faa, viral.2.protein.faa, and viral.3.protein.faa to viral.protein.faa and specified the file path in the multiPhATE.config. Please let me know how to address it. Thanks in advance. Looking forward to hearing from you asap

Best, Yongkyu

BLAST Database error: No alias or index file found for protein database [/home/ykim/software/multiPhATE-master/Databases/NCBI/Virus_Protein/viral.protein.faa] in search path [/home/ykim/software/multiPhATE-master::]

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outbig commented 4 years ago

Thank you Carol. It's workding!

carolzhou commented 4 years ago

Thank you for using multiPhATE.

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On Mar 23, 2020, at 3:50 PM, Yongkyu Kim notifications@github.com wrote:



Thank you Carol. It's workding!

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