carpentries-incubator / pangenomics

Pangenome Analysis in Prokaryotes Lesson
https://carpentries-incubator.github.io/pangenomics/
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Annotation episode #45

Closed Czirion closed 1 year ago

Czirion commented 1 year ago

Don't delete prokka outputs

solnavss commented 1 year ago

Change | Exercise 1: Inspecting the GBK. Instead of doing it with the GBK use the GFF.

solnavss commented 1 year ago

Consideration | Make a script with the Prokka 8 minutes run. Pros: save stdin and stdout, estimate run time and use nohup or screen just in case ;)

nano prokka.sh

echo "Job started at:" && date

while read line; do prokka agalactiae_$line/*.fna --kingdom Bacteria --genus Streptococcus --species agalactiae --strain $line --usegenus --addgenes --prefix Streptococcus_agalactiae_${line}_prokka --outdir ~/pan_workshop/results/annotated/Streptococcus_agalactiae_${line}_prokka; done < TettelinList.txt

echo "Job finished successfully at:" && date

./prokka.sh 2 > prokka.err 1 > prokka.out

Czirion commented 1 year ago

I removed the part where we move the gbks and delete the rest.

I updated the exercise to use the gffs instead of the gbks.

Czirion commented 1 year ago

The subsequent episodes should update the paths to the prokka outputs that they use. @solnavss @nselem @HaydeePeruyero @shadayguerrero

Czirion commented 1 year ago

Instead of making a script with prokka, use Screen when running prokka DONE