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catalystneuro / leifer_lab_to_nwb

Conversion scripts for the Leifer lab. Includes the publication Neural signal propagation atlas of Caenorhabditis elegans (Nature, 2023).
BSD 3-Clause "New" or "Revised" License
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Intensity of optogenetic stimulus? #6

Closed CodyCBakerPhD closed 2 weeks ago

CodyCBakerPhD commented 3 months ago

Details are given for intensity of the calcium imaging in the methods section, but not for the optogenetic stimulation

emosb commented 3 months ago

I can measure this to check that it is still accurate, but in the methods of the Randi paper it is reported that "the power of the laser at the sample was 1.2 mW at 500 kHz" which is referring to the optogenetic stimulus laser pulse

CodyCBakerPhD commented 3 months ago

@emosb That is the power, which I am indeed using - the intensity would be something in mW/mm^2, like in the paragraph from the methods section

For functional connectivity experiments, an intensity of 1.4 mW mm−2 at the sample plane was used to image GCaMP6s...

CodyCBakerPhD commented 2 months ago

@emosb Unless you're saying the intensity is the same in the ogen case as in the imaging case because it's the same laser?

CodyCBakerPhD commented 2 weeks ago

Leaving this empty since it would be too complicated and not worthwhile to report