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catdesk / seqtrace

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Y-scale adjustment bar is non-functional in fwd/rvs trace alignment #3

Closed catdesk closed 8 years ago

catdesk commented 8 years ago

Issue by GoogleCodeExporter Tuesday Mar 29, 2016 at 22:33 GMT Originally opened as https://github.com/catdesk/seqtrace-a/issues/3

What steps will reproduce the problem?

1. Once the final alignment is made between forward and reverse trace files, 
the y-scale bar no longer is adjustable. If the y-scale bar remained functional 
it would be helpful. 
2.
3.

What is the expected output? What do you see instead?

The y-adjust bar is always stuck in the final view of forward and reverse 
together.  In most instances the trace file is scaled acceptably so that I can 
see both forward and reverse peaks to judge each base call.  However, in some 
sequences, once the forward and reverse are put together, the y-bar is turned 
all the way down, and the peaks are too low to read.  If I could raise the 
peaks it would be helpful. 

What version of the product are you using? On what operating system?
Version 0.8.1 for windows

Please provide any additional information below.

The bar works fine when an individual trace (only forward or only reverse) is 
being viewed.  It is only stuck when the two are put together. 

I have attached the two trace files (ITS1 is forward, ITS4 is reverse) that I 
had the problem with in which the peaks were too low.  However, note that the 
y-adjust gets stuck in all files, not just these.  It became a problem with 
these because due to the low y-scale the peaks are too low to read.

Original issue reported on code.google.com by czamb...@gmail.com on 8 Jun 2013 at 3:40

Attachments:

catdesk commented 8 years ago

Comment by GoogleCodeExporter Tuesday Mar 29, 2016 at 22:33 GMT

Thanks for your comments!  In the trace view window, the y-axis scale for each 
trace file must be adjusted separately (which is usually what you want to do, 
anyway).  Make sure that either "Forward:" or "Reverse:", not "Both:", is 
selected on the trace view window toolbar, and you should be able to adjust the 
y-axis scale just fine.  I wrote a more detailed answer for this in the FAQ: 
http://code.google.com/p/seqtrace/wiki/FAQ?ts=1390095034&updated=FAQ#Why_can%27t
_I_adjust_the_y-axis_scale_when_I%27m_viewing_f.

Original comment by stucky.b...@gmail.com on 19 Jan 2014 at 1:35