cdanielmachado / carveme

CarveMe: genome-scale metabolic model reconstruction
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The input genome did not match sufficient genes/reactions in the database. #163

Open AnisMansouri opened 2 years ago

AnisMansouri commented 2 years ago

Hi Everybody,

I tried to run CarveMe using DNA data (my_genome.fna: https://www.ncbi.nlm.nih.gov/genome/?term=txid4959[orgn]):

carve --dna my_genome.fna

I got this message:

The input genome did not match sufficient genes/reactions in the database.

Note: I tried several genomes and I got the same message. However, the model was successfully built using FASTA protein file (.faa) as input.

cdanielmachado commented 2 years ago

Hi,

You can use DNA files as long as they are already annotated into individual genes.

CarveMe doesn't do ORF annotation, because there are already many good tools to do that. If you have a raw genome you would have to first use prokka/prodigal.