A mettre sous forme de modules, mais voici un exemple de résultat:
Figure 3. --- Paramacrobiotus sp. shows fluorescence under UV light. (A) Fluorescence microscope images of Paramacrobiotus sp., its moulted cuticle and egg are shown. UV-sensitive H. exemplaris was used as control for comparison. All images were taken under identical microscope settings. (B) Fluorescence images of the total lysates of Paramacrobiotus sp. and H. exemplaris under UV light (365 nm and 254 nm) are shown. Paramacrobiotus sp. lysate shows fluorescence that is stable even after proteinase K treatment.
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==> To test if the UV resistant property of Paramacrobiotus sp. can be transferred to UV sensitive H. exemplaris or C. elegans, three hundred individuals of Paramacrobiotus sp. were homogenized in 120 μl of water using a mechanical tissue grinder. This was followed by centrifugation of the lysate at 20,000 g for 15 min. The supernatant showed fluorescence under UV light (wavelength 254 nm and 365 nm) and 40 μl of it was added in a well of a 96-well plate. 40 μl of sterile water was used as control. Twenty individuals of H. exemplaris or 50 individuals of C. elegans were added in those wells, and exposed to UV-C radiation (1 kJ/m2) as described above. They were monitored every day after the treatment.
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==> Incidentally, Paramacrobiotus sp. showed strong fluorescence under UV illumination (DAPI channel, Axio Observer.Z1). This fluorescence was absent in H. exemplaris, which were sensitive to UV radiation. Similar fluorescence was observed in the eggs of Paramacrobiotus sp., but not on its moulted cuticle (Fig 3A). Extract from Paramacrobiotus sp. obtained after homogenizing the organisms in tissue lysis buffer also showed strong fluorescence under UV illumination (254 nm and 365 nm). The fluorescence was absent in the extract from H. exemplaris. The fluorescence was intact even after proteinase K treatment of the lysate for one hour suggesting that the fluorescent compound is not a protein (Fig 3B).
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==> (A) Fluorescence microscope images of Paramacrobiotus sp., its moulted cuticle and egg are shown. UV-sensitive H. exemplaris was used as control for comparison. All images were taken under identical microscope settings. (B) Fluorescence images of the total lysates of Paramacrobiotus sp. and H. exemplaris under UV light (365 nm and 254 nm) are shown. Paramacrobiotus sp. lysate shows fluorescence that is stable even after proteinase K treatment.
A mettre sous forme de modules, mais voici un exemple de résultat: