For multi-enzyme digests which produce varying length ligation products, use of the longest in estimating the observed fraction will lead to overestimation.
A more accurate value could be calculated as a weighted sum over the observed abundance of each possible junction. This information is already collected.
For multi-enzyme digests which produce varying length ligation products, use of the longest in estimating the observed fraction will lead to overestimation.
A more accurate value could be calculated as a weighted sum over the observed abundance of each possible junction. This information is already collected.