Closed qiaochen closed 3 years ago
And where is the Cell disease state that mentioned in README?
I agree that the merging procedure is not described and it is really hard to reproduce it from the raw data (in GEO). Maybe, you could provide us with all_datasets.pkl
or all_data.mtx
files to get the annotation right. On the other hand, the disease state is fully tractable from the sample names ("MS" vs "PTC/PST" patterns) and isMS
/isCSF
variables (given the full/merged dataset is provided).
Would be able to kindly provide us with further instructions? Thanks in advance!
├── MS19270 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── MS49131 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── MS58637 │ └── CSF │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── MS60249 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── MS71658 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── MS74594 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── PST45044 │ └── CSF │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── PST83775 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── PST95809 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── PTC32190 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx ├── PTC41540 │ ├── CSF │ │ └── GRCh38 │ │ ├── barcodes.tsv │ │ ├── genes.tsv │ │ └── matrix.mtx │ └── PBMCs │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx └── PTC85037 ├── CSF │ └── GRCh38 │ ├── barcodes.tsv │ ├── genes.tsv │ └── matrix.mtx └── PBMCs └── GRCh38 ├── barcodes.tsv ├── genes.tsv └── matrix.mtx
Here is the data structure for using the dataset.ipynb. The bardocdes, genes, and matrix files are in the Supplementary Files in the geo accession. Since GEO does not accept identical file names, the files are named by their full path: Essentially I replaced all "/" with "_".
'PCT32190_CSF', 'PTC41540_CSF', 'PST45044_CSF', 'PTC85037_CSF', 'MS58637_CSF', 'MS19270CSF', 'MS71658CSF', 'MS49131CSF' are the samples used in the CSF comparisons.
Hi, I am trying to execute the code, but am stuck in the Datasets.ipynb notebook. Could you please elaborate on how the raw data are arranged in the folder.
Are the CSF datasets merged into one single file? How is the file "/data/yosef2/users/chenling/CSF/CSF_data/celltypes.txt" generated?
Thanks!