Segment fault assembled the Hifi reads with UL-ont reads

[wangsb111]

     Hello:   I try to assmebly the hifi reads with the Ul-ont reads( filtered the reads length less than 50kb) 。 The previous steps were run  well , but its crashed after the *bin file gnerated.  149Gb hifireads 、80Gb Ul-ont reads、hifiasme(v:0.18.2)。

commad lines: Hifi_asm/hifiasm-0.18.2/hifiasm --primary -t 100 -a 4 -m 10000000 -D 7 -N 200 -o Goat hifi1.clean.fasta.gz hifi2.clean.fasta.gz hifi3.clean.fasta.gz hifi4.clean.fasta.gz --ul ul-ont1.fq ul-ont2.fq ul-ont3.fq ul-ont4.fq . the Ul-ont reads are fq file which contains the information of base quality.

[chhylp123]

With your command, hifiams will regard UL reads as HiFi reads as well. Please try:

hifiasm --primary -t 100 -a 4 -m 10000000 -D 7 -N 200 -o Goat --ul ul-ont1.fq,ul-ont2.fq,ul-ont3.fq,ul-ont4.fq hifi1.clean.fasta.gz hifi2.clean.fasta.gz hifi3.clean.fasta.gz hifi4.clean.fasta.gz