Reads filter

[pxxiao-hz]

Hello,

Is there a minimum length limit for UL and HiFi reads? Should the reads be filtered by metrics such as length and quality before using hifiasm?

Best wishes.

[chhylp123]

It would be better to filter out too short UL reads. Cutoffs like 50kb or 100kb work in our tests.

[pxxiao-hz]

Yes, in my case, 50kb was better.

Have you tested the deepth of reads?

[chhylp123]

Our preprint is here: https://arxiv.org/abs/2306.03399. I feel like 20x UL would work even for large genomes.