Hello,
I ran hifiasm on my pacbio Hifi raw data with the following command:
hifiasm -o it20_007_hifi.asm -t 40
Before I made a genome size and heterozygosity estimation of my raw data using jellyffish and genomescope which gave me a high heterozygosity for my file why I wanted to go on with the phased contig sets.
In the documentation of hifiasm I read that hifiasm also produces two sets of partially phased contigs without additional data as Hi-C or trio but I couldnt find a it20_007.asm.bp.hap?.p_ctg.gfa. file in my output. Have I done sth wrong? Or do I have to add another parameter?
Hello, I ran hifiasm on my pacbio Hifi raw data with the following command:
hifiasm -o it20_007_hifi.asm -t 40
Before I made a genome size and heterozygosity estimation of my raw data using jellyffish and genomescope which gave me a high heterozygosity for my file why I wanted to go on with the phased contig sets. In the documentation of hifiasm I read that hifiasm also produces two sets of partially phased contigs without additional data as Hi-C or trio but I couldnt find a it20_007.asm.bp.hap?.p_ctg.gfa. file in my output. Have I done sth wrong? Or do I have to add another parameter?
Thats the output I got:
it20_007_hifiasm.a_ctg.gfa
it20_007_hifiasm.ovlp.reverse.bin
it20_007_hifiasm.p_ctg.fa.fai
it20_007_hifiasm.p_utg.gfa
it20_007_hifiasm.r_utg.noseq.gfa it20_007_hifiasm.a_ctg.noseq.gfa
it20_007_hifiasm.ovlp.source.bin
it20_007_hifiasm.p_ctg.gfa
it20_007_hifiasm.p_utg.noseq.gfa
it20_007_hifiasm.ec.bin
it20_007_hifiasm.p_ctg.fa
it20_007_hifiasm.p_ctg.noseq.gfa it20_007_hifiasm.r_utg.gfa
Thanks for your help.
Best regards
Male Arimond