chhylp123 / hifiasm

Hifiasm: a haplotype-resolved assembler for accurate Hifi reads
MIT License
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No set of partially phased contigs #472

Open malearimond opened 1 year ago

malearimond commented 1 year ago

Hello, I ran hifiasm on my pacbio Hifi raw data with the following command:

hifiasm -o it20_007_hifi.asm -t 40

Before I made a genome size and heterozygosity estimation of my raw data using jellyffish and genomescope which gave me a high heterozygosity for my file why I wanted to go on with the phased contig sets. In the documentation of hifiasm I read that hifiasm also produces two sets of partially phased contigs without additional data as Hi-C or trio but I couldnt find a it20_007.asm.bp.hap?.p_ctg.gfa. file in my output. Have I done sth wrong? Or do I have to add another parameter?

Thats the output I got:

it20_007_hifiasm.a_ctg.gfa
it20_007_hifiasm.ovlp.reverse.bin
it20_007_hifiasm.p_ctg.fa.fai
it20_007_hifiasm.p_utg.gfa
it20_007_hifiasm.r_utg.noseq.gfa it20_007_hifiasm.a_ctg.noseq.gfa
it20_007_hifiasm.ovlp.source.bin
it20_007_hifiasm.p_ctg.gfa
it20_007_hifiasm.p_utg.noseq.gfa
it20_007_hifiasm.ec.bin
it20_007_hifiasm.p_ctg.fa
it20_007_hifiasm.p_ctg.noseq.gfa it20_007_hifiasm.r_utg.gfa

Thanks for your help.

Best regards

Male Arimond

chhylp123 commented 1 year ago

So sorry for the late reply. Could you please show the command lines?