how to estimate the homo-coverage for captured HiFi sequencing data

chhylp123 / hifiasm

Hifiasm: a haplotype-resolved assembler for accurate Hifi reads

MIT License

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how to estimate the homo-coverage for captured HiFi sequencing data #682

Open wangBiojin opened 4 months ago

wangBiojin commented 4 months ago

Hi,

I am working on a published dataset for Ren-seq HiFi data of tomato. This sequencing technology captures the genomic regions with Resistance genes. When analyzing the assembly, I am unable to observe the classic two peaks in the Kmer plots. I have reviewed comments #221. Can you provide guidance for a similar dataset now? Any advice will be greatly appreciated.

Thanks