chhylp123 / hifiasm

Hifiasm: a haplotype-resolved assembler for accurate Hifi reads
MIT License
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Incomplete haplotype-level assembly with hic #685

Open dandanWang2019 opened 3 months ago

dandanWang2019 commented 3 months ago

Hi,

My assembly showed the balanced haplotypes without HiC data (both: BUSCO of 90.1%). However, the HiC data integrated separated imbalanced assemblies (hap1: C:87.8%[S:86.4%,D:1.4%],F:0.7%,M:11.5%; hap2: C:97.6%[S:96.5%,D:1.1%],F:0.6%,M:1.8%). The hap1 showed larger missing BUSCO value compared to hap2. In addition, the hap1 (~430 Mb) is ~50 Mb shorter than the hap2 (~484 Mb).

I wonder if this is a big difference between these two haplotypes and what are the possibilities for this difference?