Closed Midsummer723 closed 2 years ago
Hi @Midsummer723 -- difficult to diagnose without more information. Have you verified that gt.calls is properly set?
Thank you very much for your reply, but I still have some problems: 1.I find the when use the sctranfrom to process the data. When run the "sweep.res.list_kidney <- paramSweep_v3(seu_kidney, PCs = 1:10, sct = FALSE)" , it will come "Error in NormalizeData.default(object = GetAssayData(object = object, : trying to get slot "params" from an object of a basic class ("NULL") with no slots"
Hi @Midsummer723 ,
Responses to each point below:
2-4: Please provide the exact code you are using so I can more effectively troubleshoot.
Thank you for the great tool. I have a problem when I run the ' > gt.calls <- seu_kidney@meta.data[rownames(sweep.res.list_kidney[[1]]), "GT"] ## GT is a vector containing "Singlet" and "Doublet" calls recorded using sample multiplexing classification and/or in silico geneotyping results