Im encountering an error related to the --splint_file input. The output indicates it's treating the first splint header as a path to open?
$ C3POa --reads qc_fastqs/qc_test.fastq --splint_file ./splints.fa --out_path qc_fastqs/ --numThreads 8
C3POa v3 - It's over Anakin. I have the consensus!
Generating consensus sequences from R2C2 read data
Starting consensus calling iteration - if input is directory it will check for files that are new since last iteration
Read input is a file
1 file(s) provided
Couldn't open >UMI_Splint_1_F , No such file or directory
Im encountering an error related to the --splint_file input. The output indicates it's treating the first splint header as a path to open?
The splint file is:
It seems like the file is being read in with mm.fastx_read correctly. If I add
print(splint_dict)
after line 177 of C3POa.py I see:{'UMI_Splint_1_F': ['ACTCTGCGTTGATACCACTGCTTTGAGGCTGATGAGTTCCATANNNNNTATATNNNNNATCACTACTTAGTTTTTTGATAGCTTCAAGCCAGAGTTGTCTTTTTCTCTTTGCTGGCAGTAAAAG', 'CTTTTACTGCCAGCAAAGAGAAAAAGACAACTCTGGCTTGAAGCTATCAAAAAACTAAGTAGTGATNNNNNATATANNNNNTATGGAACTCATCAGCCTCAAAGCAGTGGTATCAACGCAGAGT'], 'UMI_Splint_1_R': ['ACTCTGCGTTGATACCACTGCTTAAAGGGATATTTTCGATCGCNNNNNATATANNNNNTTAGTGCATTTGATCCTTTTACTCCTCCTAAAGAACAACCTGACCCAGCAAAAGGTACACAATACTTTTACTGCCAGCAAAGAG', 'CTCTTTGCTGGCAGTAAAAGTATTGTGTACCTTTTGCTGGGTCAGGTTGTTCTTTAGGAGGAGTAAAAGGATCAAATGCACTAANNNNNTATATNNNNNGCGATCGAAAATATCCCTTTAAGCAGTGGTATCAACGCAGAGT']}