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churchlab / millstone

Genome engineering and analysis software
http://churchlab.github.io/millstone/
MIT License
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Generate New Reference Genome: ERROR: More than one record found in handle #699

Open bfurtwa opened 6 years ago

bfurtwa commented 6 years ago

I put all variants in a new variant set and generated a new reference genome using this variant set. When I click submit, I get the error: "ERROR: More than one record found in handle"

glebkuznetsov commented 6 years ago

Does your original reference genome have multiple records? Are you able to share it? Thanks!

bfurtwa commented 6 years ago

I used NZ_CP009974 and the plasmid NZ_CP009975 in one .gb file produced by geneious 8

glebkuznetsov commented 6 years ago

Got it. It's likely we didn't test the case of generating a new reference genome for the combined genome/plasmid case, but the fix may be straightforward.

Do you mind attaching the combined .gb file?

On Thu, Mar 8, 2018 at 5:45 AM Benjamin Furtwängler < notifications@github.com> wrote:

I used NZ_CP009974 and the plasmid NZ_CP009975 in one .gb file produced by geneious 8

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bfurtwa commented 6 years ago

NZ_CP009975_4.zip

glebkuznetsov commented 6 years ago

Thanks. We'll look into fixing this issue with versioning reference genomes with multiple records (e.g. w/ plasmid).

In the meantime, if you need the versioned genomed, you may consider aligning to the reference with NZ_CP009974 only.

On Thu, Mar 8, 2018 at 8:49 AM Benjamin Furtwängler < notifications@github.com> wrote:

NZ_CP009975_4.zip https://github.com/churchlab/millstone/files/1793368/NZ_CP009975_4.zip

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