Problems trying to generate kymographs for lumen opening data

[clairelbromley]

Building kymographs for 270416, Embryo 1 downwards, cut 1 Saving overlay images... Error using set Width and height must be > 0

Error in plotAndSaveKymographsSlow (line 162) set(hax1, 'Position', new_ax1pos);

Error in cziFig>buttonGenerateKym_Callback (line 308) kymographs = plotAndSaveKymographsSlow(trim_stack, curr_metadata, userOptions);

Error in gui_mainfcn (line 95) feval(varargin{:});

Error in cziFig (line 42) gui_mainfcn(gui_State, varargin{:});

Error in @(hObject,eventdata)cziFig('buttonGenerateKym_Callback',hObject,eventdata,guidata(hObject))

Error while evaluating uicontrol Callback

[clairelbromley]

[clairelbromley]

Rolled code back to lumenopeningbasalmembrane (7 June 2017), to see if this would work In this case the kymographs were generated, but instead of cropping to the chosen region, black pixels were inserted