clairelbromley / ZebrafishKymographing

Various software tools written for analysis of lumen opening and membrane retraction in zebrafish.
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Problems with multipage fast tif exports #56

Closed clairelbromley closed 7 years ago

clairelbromley commented 7 years ago

Am sure we made this possible to do before, but I can't find the section of the code where I can change the cross size in multipage tiff exports Please can you have a look?

dougkelly88 commented 7 years ago

For slow multipage tiffs, the cross sizes are hardcoded in testCutPositioningSlow.m, line 9. For fast multipage tiffs, the cross sizes are hardcoded in testCutPositioningFast.m, line 9. Let me know if this is something that would be useful to make accessible in the list of user options at the top of kymographBase.

On 12 December 2016 at 14:55, clairelbromley notifications@github.com wrote:

Assigned #56 https://github.com/clairelbromley/ZebrafishKymographing/issues/56 to @dougkelly88 https://github.com/dougkelly88.

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clairelbromley commented 7 years ago

It would be great if it could be added to the list of user options at the start of kymographBase :) thanks

On 12 December 2016 at 15:11, dougkelly88 notifications@github.com wrote:

For slow multipage tiffs, the cross sizes are hardcoded in testCutPositioningSlow.m, line 9. For fast multipage tiffs, the cross sizes are hardcoded in testCutPositioningFast.m, line 9. Let me know if this is something that would be useful to make accessible in the list of user options at the top of kymographBase.

On 12 December 2016 at 14:55, clairelbromley notifications@github.com wrote:

Assigned #56 https://github.com/clairelbromley/ZebrafishKymographing/issues/56 to @dougkelly88 https://github.com/dougkelly88.

— You are receiving this because you were mentioned. Reply to this email directly, view it on GitHub https://github.com/clairelbromley/ZebrafishKymographing/issues/ 56#event-890469013, or mute the thread https://github.com/notifications/unsubscribe-auth/ ADxBsH4nSxoA2F1c2md9F5ChWUFZ4CXFks5rHWBFgaJpZM4LKoda .

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clairelbromley commented 7 years ago

Is it possible to also make the lines thicker?

screen shot 2016-12-12 at 15 42 00

I altered lines 29 and 30, and made all the 5s, 10s I couldnt see anything else that was obviously changeable - but tbh not sure I'd know! screen shot 2016-12-12 at 15 32 14

clairelbromley commented 7 years ago

The latest version of the code changes the cross sizes, however only exports one multipage tiff like the ones from testCutPositioningSlow.m Currently I use the multipage tiffs produced from testCutPositioningFast.m, and these fast tiffs are not created by the current code. Would it be possible to toggle between these two?

The changing cross size seems to be working 👍

dougkelly88 commented 7 years ago

Should now work, 4cf4104f3d41c6ca1b37d66dfc46fd441c5421e1 and b3412c77ec346b0fd036fb418f229771bd9ff094. Close the issue if it behaves?

clairelbromley commented 7 years ago

Still doesn't produce the multipage tiffs produced from testCutPositioningFast.m

Crosses sizes seem to vary when changing size in kymoBase - but can test properly once the export files are the correct ones

clairelbromley commented 7 years ago

190716, Embryo 8, Cut 1, multipage fast

Multipage fast tifs now save, however the laser cut is positioned in the wrong location. The multipage tifs have the cut in the right place

screen shot 2017-01-09 at 14 03 39