clairelbromley / ZebrafishKymographing

Various software tools written for analysis of lumen opening and membrane retraction in zebrafish.
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Enhancements to cziFig: bleach data #75

Closed clairelbromley closed 7 years ago

clairelbromley commented 7 years ago

For analysis of bleach data: 1) Is it possible to put a blacked out region in the kymograph at the time point when bleaching occured? (or something similar - perhaps a blue line?)

2) Change analysis so can choose to only do quantitative calculation after bleaching

3) Set default median filter size as 1

4) Check that both median filtering and erosion/dilation are definitely turned off by setting median filter size to 1

5) Ability to change kymograph widths in user settings

clairelbromley commented 7 years ago

Also add ability to change kymograph length

clairelbromley commented 7 years ago

(double check y axis (position relative to cut) is correct and scales with changing pixel sizes)

clairelbromley commented 7 years ago

?? How easy would it be to draw more than one line in an image- to be able to look at bleached basal, apical and unbleached basal

(also - is there a way to show which way the up and down kymograph settings will be, before starting the analysis?)

clairelbromley commented 7 years ago

When I move slider date and embryo number go back to preset data

clairelbromley commented 7 years ago

Is there a way to minimise the files popping up from matlab? Don't worry if not easy- but think we already managed to do this for kymbase

clairelbromley commented 7 years ago

Make noise when cziFig has finished kymograph processing?

dougkelly88 commented 7 years ago

It's easiest (and makes it feel like I'm making progress) if each feature request has it's own issue raised...x

clairelbromley commented 7 years ago

Put as separate issues :)