Closed isaacambrogetti closed 4 months ago
Thanks for using VRPG and giving feedback. Mingraph-cactus uses GFA 1.1 (https://github.com/ComparativeGenomicsToolkit/cactus/blob/master/doc/pangenome.md) , which is different from rGFA (https://github.com/lh3/gfatools/blob/master/doc/rGFA.md). Descriptions of the options '-cxasm --vc' of minigraph can be found by run './minigraph' (without any options) directly from the console. For graph in GFA format created by minigraph-cactus the graph should be converted to a rGFA-like format by gfa2view as described in the document before visualization. The error message 'GFA file open failed' generally means that the file path of GFA file may be incorrect.
Thank you for answering and for correcting the important misunderstanding I had with the Minigraph-Cactus output.
My issue was indeed due to an incorrect file path.
Hello, I'm having an issue while loading my .gfa file of a pangenome I created using the mingraph-cactus pipeline.
For context it's a pangenome created from 1 reference + 2 genomes with the following characteristics:
I am trying to run the command at the step Pangenome graph already exists, but I do not have the .list files it requires and I cannot find any documentation regarding the minigraph command
-cxasm --vc
.I was wondering if you have any suggestion on how to behave when dealing with a file rGFA from the minigraph-cactus pipeline.
Furthermore, I tried to work directly from the step GFA format graph but the
Error: GFA file open failed
araised, which I think it might be due to the fact that I am dealing with a rGFA file and not a GFA.Thank you in advance! Isaac