Xiaojieqiu
commented
6 years ago Hi to-arai, you can try to perform the clustering analysis on the trajectory with the density peak clustering algorithm
Open to-arai opened 6 years ago
Xiaojieqiu
commented
6 years ago Hi to-arai, you can try to perform the clustering analysis on the trajectory with the density peak clustering algorithm
Hi,
I've obtained single-cell NGS data of "non-model" organism (plants) and want to cluster the cells in pseudotime, subsequently to estimate a phase transition along with pseudotime. I need an unsupervised clustering way because there is no marker gene to cluster cell properly in pseudotime.
I learn to know that monocle has some unsupervised clustering methods and got a trajectory using dpFeature, which seemed the most suitable to me. But clusters from density peak clustering don't match with trajectory at all.
Do you have any idea or suggestion to obtain more feasible result in such situation ?
Best