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Isolation of Fungal Strains from Soil Samples: Solid potato dextrose agar (PDA) media were used for the isolation and identification of fungal strains. The inoculum prepared from soil samples was spread on PDA medium, and incubation was carried out at 28 ± 2°C for 5 to 7 days. After incubation, the morphology of the colonies was recorded to identify the specific fungus collected in each sample (ref) [https://www.mdpi.com/2304-8158/10/6/1280]. For bacteria: Starch casien agar(https://www.nature.com/articles/202928a0). We prepared a mineral solution composed of 2 g/L (NH4)2SO4, 4 g/L KH2PO4, 6 g/L Na2HPO4, 0.05 g/L CaCl.2H2O, 0.2 g/L MgSO4.7H2O, and 0.01 g/L FeSO4.7H2O (https://link.springer.com/article/10.1007/s11270-016-2789-z).
List relevant bibliography (report DOI + relevant sections) in a new Dendron note (for example projects.commons-public.microcosmics.bibliography)
Soil metabolomics
Sample prepraration
Mass Spectrometry based metabolomics
Microcosms
Usecase of biolog Ecoplate
Quick and dirty microbials community chracterization