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commons-research / microcosmics

A dump repo for the microcosmics project
MIT License
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Determination of Soil Microbial Biodiversity #6

Open rima-bio opened 9 months ago

rima-bio commented 9 months ago
  1. Through amplicon sequencing (Illumina MiSeq): The diversity of soil bacteria and fungi was analyzed through amplicon sequencing (Illumina MiSeq). A total of 10 g of frozen soil samples were cooled using liquid nitrogen and ground using a mortar and pestle. Soil DNA (n = 435) was extracted using a DNA Isolation Kit (Powersoil, MoBio Laboratories, Carlsbad, CA, USA). A portion of the bacterial 16S and eukaryotic 18S rRNA genes were sequenced using the 515F/806R and Euk1391f/EukBr primer sets, respectively (https://doi.org/10.1038/s41396-021-00906-0.