Protocol
The community reaction patterns are typically analyzed at defined time intervals over 2 to 5 days.
TYPICAL PROCEDURE
STEP 1: Environmental samples are inoculated directly into EcoPlates either as aqueous samples or after suspension (soil, sludge, sediment, etc.).
STEP 2: The EcoPlates are incubated and kinetic data collected and analyzed.
STEP 3: The community-level physiological profile is assessed for key characteristics:
o Pattern development (similarity)
o Rate of color change in each well (activity)
o Richness of well response (diversity)
The reaction patterns are most effectively analyzed using an Odin™ system for Phenotype Characterization. Odin can incubate and read up to 50 plates at once, and automatically generates kinetic analyses for each condition.
Statistical analysis of the data is typically performed using standard software packages. Some researchers have found that PCA provides greater resolution than other methods of statistical analysis11.
Formation of purple color occurs when the microbes can utilize the carbon source and begin to respire. The respiration of the cells in the community reduces a tetrazolium dye that is included with the carbon source.
(chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.biolog.com/wp-content/uploads/2023/08/00A-012-Rev-F-EcoPlate-IFU.pdf).
Protocol The community reaction patterns are typically analyzed at defined time intervals over 2 to 5 days. TYPICAL PROCEDURE STEP 1: Environmental samples are inoculated directly into EcoPlates either as aqueous samples or after suspension (soil, sludge, sediment, etc.). STEP 2: The EcoPlates are incubated and kinetic data collected and analyzed. STEP 3: The community-level physiological profile is assessed for key characteristics: o Pattern development (similarity) o Rate of color change in each well (activity) o Richness of well response (diversity) The reaction patterns are most effectively analyzed using an Odin™ system for Phenotype Characterization. Odin can incubate and read up to 50 plates at once, and automatically generates kinetic analyses for each condition. Statistical analysis of the data is typically performed using standard software packages. Some researchers have found that PCA provides greater resolution than other methods of statistical analysis11. Formation of purple color occurs when the microbes can utilize the carbon source and begin to respire. The respiration of the cells in the community reduces a tetrazolium dye that is included with the carbon source. (chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.biolog.com/wp-content/uploads/2023/08/00A-012-Rev-F-EcoPlate-IFU.pdf).