micca v1.7.2 run via docker on a mac.
We had been working through the default paired-end workflow but realized or 'R2' files had forward primer and vice-versa so we changed the i,p&e flags in micca mergepairs as follows: -i <path>/*_R2*.fastq -p _R2 -e _R1 and got the following error:
VSEARCH error: vsearch v 2.7.1 Fatal error: Invalid line 172 in FastQ Illegal character ' '
However, the analysis continued to run and produced a merged fastq around the expected size and this was used in successful trim, filterstats, and filter commands subsequently. However, at the micca otu stage, we again encountered a VSEARCH error:
This command produced a folder, but no files. I note that the vsearch errors started appearing when we changed up the forward pattern and my colleague wonders if she might be running into storage/processing limitations of her machine. What do you think? Have you heard of this before? The input filtered.fasta file looks similar to others we have used successfully. Thanks
micca v1.7.2 run via docker on a mac. We had been working through the default paired-end workflow but realized or 'R2' files had forward primer and vice-versa so we changed the i,p&e flags in micca mergepairs as follows:
-i <path>/*_R2*.fastq -p _R2 -e _R1and got the following error: VSEARCH error: vsearch v 2.7.1 Fatal error: Invalid line 172 in FastQ Illegal character ' ' However, the analysis continued to run and produced a merged fastq around the expected size and this was used in successful trim, filterstats, and filter commands subsequently. However, at the micca otu stage, we again encountered a VSEARCH error:This command produced a folder, but no files. I note that the vsearch errors started appearing when we changed up the forward pattern and my colleague wonders if she might be running into storage/processing limitations of her machine. What do you think? Have you heard of this before? The input filtered.fasta file looks similar to others we have used successfully. Thanks