I made a database by using metagenomic sequencing data. However, when I chose this home-made fasta file as the database and run "start searching", it was always said "searching database failed", and in the results, no peptide or protein was identified. May I know why it is like that? Is it only the public database with strict formating can be used? Thank you for your attention and time.
The formt of sequences in the home-made database is as follows:
C1_00001 Electron transport complex subunit RsxG
MAHSDTPGISDPAYENVPQRIVQANSPQVEIASGATVSSKAIMAAVEQALAQAXXXXXXX
XXXXVSSKAIMAAVEQALAQAVDGAATEAAVEAFTIAVDDGTYRASADGFGGELVIDVTV
EGGKITDIEVVESQETPFIADTAFKELIPAIIEAQGPVEAVSGATFTSKALLEAVDKAVS
GVTEASKAAEPFAILVEDGTYRGSADGFGGELVIDVTVEGGKITNIEVVQSEETPFIADT
AFKHLIPAIIETQGPVEAVSGATFISQALEDAVRDALRIKEGQ
C1_00002 Electron transport complex subunit RsxE
MKQLWEGLFTKNPVLVLALGLVPAVAVTTTAMNGLALGIIAAILLVAAAIVNWVLAPHVP
ENAKLAVRMLVLILLVVAVYSLLLGRNPGLVASLGIFLPLLAVDELLLQAGGERRGFGEE
LLRACGQGLGFALVLVVLGVVREFLGMGSIFGRQIVTGSLAPLSLAGSVPGGMVILGLLM
ALVNLVTKRGGELHD
I made a database by using metagenomic sequencing data. However, when I chose this home-made fasta file as the database and run "start searching", it was always said "searching database failed", and in the results, no peptide or protein was identified. May I know why it is like that? Is it only the public database with strict formating can be used? Thank you for your attention and time.
The formt of sequences in the home-made database is as follows: