mvaudel
commented
5 years ago Hi,
Apologies that the tool did not manage to load your data, and that the error message did not help. It seems like an issue with our back-end database. We are in the process of rewriting this part of the code but cannot give an ETA on the release currently. In the meantime, we can only recommend to increase the memory available for the tool or reduce the number of search engines used.
Sorry that we cannot help more at this stage,
Marc
hbarsnes
Hi Harald,
I have search results from Mascot, MSGF+ and X!Tandem. When I try importing the results using PeptideShaker, I get an error at the building peptide and proteins step after computing probabilities and importing gets cancelled. The error is not intuitive. Any help appreciated.
The log shows: Fri Dec 21 13:25:00 AEDT 2018: PeptideShaker version 1.16.36.
java.lang.NullPointerException at eu.isas.peptideshaker.PeptideShaker.attachSpectrumProbabilitiesAndBuildPeptidesAndProteins(PeptideShaker.java:832) at eu.isas.peptideshaker.PeptideShaker.processIdentifications(PeptideShaker.java:398) at eu.isas.peptideshaker.fileimport.FileImporter$IdProcessorFromFile.importFiles(FileImporter.java:547) at eu.isas.peptideshaker.fileimport.FileImporter$IdProcessorFromFile.doInBackground(FileImporter.java:431) at javax.swing.SwingWorker$1.call(Unknown Source) at java.util.concurrent.FutureTask.run(Unknown Source) at javax.swing.SwingWorker.run(Unknown Source) at java.util.concurrent.ThreadPoolExecutor.runWorker(Unknown Source) at java.util.concurrent.ThreadPoolExecutor$Worker.run(Unknown Source) at java.lang.Thread.run(Unknown Source) Free memory: 871597050
The report file is below:
PeptideShaker 1.16.36 Report File
Fri Dec 21 13:29:11 AEDT 2018 Import process for Sanger_fingerprint (Sample: Mutation, Replicate: 0)
Fri Dec 21 13:29:11 AEDT 2018 Importing sequences from uniprot_sprot_human_withSNPs_trypticMutant_concat_decoy.fasta. Fri Dec 21 13:29:24 AEDT 2018 FASTA file import completed. Fri Dec 21 13:29:24 AEDT 2018 Importing gene mappings. Fri Dec 21 13:29:25 AEDT 2018 Establishing local database connection. Fri Dec 21 13:29:25 AEDT 2018 Reading identification files. Fri Dec 21 13:29:25 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_01.mzid. Fri Dec 21 13:29:26 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_01.mzid. Fri Dec 21 13:29:26 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_01.mgf Fri Dec 21 13:29:27 AEDT 2018 180419_E0022_P01_pool30_I_M06_01.mgf imported. Fri Dec 21 13:29:27 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:29:27 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:29:27 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_01.mzid Fri Dec 21 13:29:29 AEDT 2018 26534 identified spectra (40.6%) did not present a valid peptide. Fri Dec 21 13:29:29 AEDT 2018 32742 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:29:29 AEDT 2018 - 1.1% peptide mapping to both target and decoy. Fri Dec 21 13:29:29 AEDT 2018 - 38.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:29:29 AEDT 2018 - 60.2% unrecognized modifications. Fri Dec 21 13:29:29 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_01.t.2018_12_12_10_49_57.t.xml. Fri Dec 21 13:30:15 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_01.t.2018_12_12_10_49_57.t.xml. Fri Dec 21 13:30:15 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:30:15 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:30:16 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_01.t.2018_12_12_10_49_57.t.xml Fri Dec 21 13:30:16 AEDT 2018 4939 identified spectra (9.9%) did not present a valid peptide. Fri Dec 21 13:30:16 AEDT 2018 6579 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:30:16 AEDT 2018 - 6.0% peptide mapping to both target and decoy. Fri Dec 21 13:30:16 AEDT 2018 - 22.5% peptide length less than 7 or greater than 30, Fri Dec 21 13:30:16 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:30:16 AEDT 2018 - 69.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:30:16 AEDT 2018 - 2.1% unrecognized modifications. Fri Dec 21 13:30:16 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_02.mzid. Fri Dec 21 13:30:18 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_02.mzid. Fri Dec 21 13:30:18 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_02.mgf Fri Dec 21 13:30:32 AEDT 2018 180419_E0022_P01_pool30_I_M06_02.mgf imported. Fri Dec 21 13:30:32 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:30:32 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:30:32 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_02.mzid Fri Dec 21 13:30:34 AEDT 2018 22831 identified spectra (34.5%) did not present a valid peptide. Fri Dec 21 13:30:34 AEDT 2018 27645 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:30:34 AEDT 2018 - 1.2% peptide length less than 7 or greater than 30, Fri Dec 21 13:30:34 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:30:34 AEDT 2018 - 42.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:30:34 AEDT 2018 - 55.8% unrecognized modifications. Fri Dec 21 13:30:34 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_02.t.2018_12_12_10_53_04.t.xml. Fri Dec 21 13:31:29 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_02.t.2018_12_12_10_53_04.t.xml. Fri Dec 21 13:31:29 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:31:29 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:31:30 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_02.t.2018_12_12_10_53_04.t.xml Fri Dec 21 13:31:30 AEDT 2018 4971 identified spectra (9.2%) did not present a valid peptide. Fri Dec 21 13:31:30 AEDT 2018 5841 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:31:30 AEDT 2018 - 4.2% peptide mapping to both target and decoy. Fri Dec 21 13:31:30 AEDT 2018 - 13.1% peptide length less than 7 or greater than 30, Fri Dec 21 13:31:30 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:31:30 AEDT 2018 - 79.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:31:30 AEDT 2018 - 3.6% unrecognized modifications. Fri Dec 21 13:31:30 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_03.mzid. Fri Dec 21 13:31:32 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_03.mzid. Fri Dec 21 13:31:32 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_03.mgf Fri Dec 21 13:31:41 AEDT 2018 180419_E0022_P01_pool30_I_M06_03.mgf imported. Fri Dec 21 13:31:41 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:31:41 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:31:41 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_03.mzid Fri Dec 21 13:31:43 AEDT 2018 25928 identified spectra (39.2%) did not present a valid peptide. Fri Dec 21 13:31:43 AEDT 2018 31612 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:31:43 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:31:43 AEDT 2018 - 39.5% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:31:43 AEDT 2018 - 58.8% unrecognized modifications. Fri Dec 21 13:31:43 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_03.t.2018_12_12_10_56_09.t.xml. Fri Dec 21 13:32:35 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_03.t.2018_12_12_10_56_09.t.xml. Fri Dec 21 13:32:35 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:32:35 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:32:35 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_03.t.2018_12_12_10_56_09.t.xml Fri Dec 21 13:32:35 AEDT 2018 5344 identified spectra (10.1%) did not present a valid peptide. Fri Dec 21 13:32:35 AEDT 2018 6872 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:32:35 AEDT 2018 - 6.3% peptide mapping to both target and decoy. Fri Dec 21 13:32:35 AEDT 2018 - 19.7% peptide length less than 7 or greater than 30, Fri Dec 21 13:32:35 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:32:35 AEDT 2018 - 71.0% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:32:35 AEDT 2018 - 3.0% unrecognized modifications. Fri Dec 21 13:32:35 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_04.mzid. Fri Dec 21 13:32:38 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_04.mzid. Fri Dec 21 13:32:38 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_04.mgf Fri Dec 21 13:32:46 AEDT 2018 180419_E0022_P01_pool30_I_M06_04.mgf imported. Fri Dec 21 13:32:46 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:32:47 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:32:47 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_04.mzid Fri Dec 21 13:32:47 AEDT 2018 27051 identified spectra (41.7%) did not present a valid peptide. Fri Dec 21 13:32:47 AEDT 2018 33206 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:32:47 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:32:47 AEDT 2018 - 38.7% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:32:47 AEDT 2018 - 59.6% unrecognized modifications. Fri Dec 21 13:32:47 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_04.t.2018_12_12_10_58_55.t.xml. Fri Dec 21 13:33:35 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_04.t.2018_12_12_10_58_55.t.xml. Fri Dec 21 13:33:35 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:33:35 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:33:35 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_04.t.2018_12_12_10_58_55.t.xml Fri Dec 21 13:33:36 AEDT 2018 5127 identified spectra (10.3%) did not present a valid peptide. Fri Dec 21 13:33:36 AEDT 2018 7041 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:33:36 AEDT 2018 - 6.9% peptide mapping to both target and decoy. Fri Dec 21 13:33:36 AEDT 2018 - 24.8% peptide length less than 7 or greater than 30, Fri Dec 21 13:33:36 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:33:36 AEDT 2018 - 66.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:33:36 AEDT 2018 - 2.2% unrecognized modifications. Fri Dec 21 13:33:36 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_05.mzid. Fri Dec 21 13:33:38 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_05.mzid. Fri Dec 21 13:33:38 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_05.mgf Fri Dec 21 13:33:48 AEDT 2018 180419_E0022_P01_pool30_I_M06_05.mgf imported. Fri Dec 21 13:33:48 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:33:48 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:33:49 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_05.mzid Fri Dec 21 13:33:49 AEDT 2018 27402 identified spectra (41.8%) did not present a valid peptide. Fri Dec 21 13:33:49 AEDT 2018 33300 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:33:49 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:33:49 AEDT 2018 - 39.2% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:33:49 AEDT 2018 - 58.9% unrecognized modifications. Fri Dec 21 13:33:49 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_05.t.2018_12_12_11_01_51.t.xml. Fri Dec 21 13:34:39 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_05.t.2018_12_12_11_01_51.t.xml. Fri Dec 21 13:34:39 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:34:40 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:34:40 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_05.t.2018_12_12_11_01_51.t.xml Fri Dec 21 13:34:40 AEDT 2018 5367 identified spectra (10.4%) did not present a valid peptide. Fri Dec 21 13:34:40 AEDT 2018 7370 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:34:40 AEDT 2018 - 6.7% peptide mapping to both target and decoy. Fri Dec 21 13:34:40 AEDT 2018 - 24.9% peptide length less than 7 or greater than 30, Fri Dec 21 13:34:40 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:34:40 AEDT 2018 - 65.8% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:34:40 AEDT 2018 - 2.6% unrecognized modifications. Fri Dec 21 13:34:40 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_06.mzid. Fri Dec 21 13:34:42 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_06.mzid. Fri Dec 21 13:34:42 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_06.mgf Fri Dec 21 13:34:52 AEDT 2018 180419_E0022_P01_pool30_I_M06_06.mgf imported. Fri Dec 21 13:34:52 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:34:52 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:34:52 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_06.mzid Fri Dec 21 13:34:52 AEDT 2018 27354 identified spectra (41.9%) did not present a valid peptide. Fri Dec 21 13:34:52 AEDT 2018 33234 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:34:52 AEDT 2018 - 39.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:34:52 AEDT 2018 - 59.5% unrecognized modifications. Fri Dec 21 13:34:52 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_06.t.2018_12_12_11_04_48.t.xml. Fri Dec 21 13:35:44 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_06.t.2018_12_12_11_04_48.t.xml. Fri Dec 21 13:35:44 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:35:44 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:35:44 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_06.t.2018_12_12_11_04_48.t.xml Fri Dec 21 13:35:44 AEDT 2018 5393 identified spectra (10.5%) did not present a valid peptide. Fri Dec 21 13:35:44 AEDT 2018 7506 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:35:44 AEDT 2018 - 5.1% peptide mapping to both target and decoy. Fri Dec 21 13:35:44 AEDT 2018 - 26.2% peptide length less than 7 or greater than 30, Fri Dec 21 13:35:44 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:35:44 AEDT 2018 - 66.2% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:35:44 AEDT 2018 - 2.6% unrecognized modifications. Fri Dec 21 13:35:44 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_07.mzid. Fri Dec 21 13:35:46 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_07.mzid. Fri Dec 21 13:35:46 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_07.mgf Fri Dec 21 13:35:56 AEDT 2018 180419_E0022_P01_pool30_I_M06_07.mgf imported. Fri Dec 21 13:35:56 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:35:56 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:35:57 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_07.mzid Fri Dec 21 13:35:57 AEDT 2018 27151 identified spectra (41.3%) did not present a valid peptide. Fri Dec 21 13:35:57 AEDT 2018 32896 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:35:57 AEDT 2018 - 1.1% peptide mapping to both target and decoy. Fri Dec 21 13:35:57 AEDT 2018 - 38.9% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:35:57 AEDT 2018 - 59.5% unrecognized modifications. Fri Dec 21 13:35:57 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_07.t.2018_12_12_11_07_48.t.xml. Fri Dec 21 13:36:48 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_07.t.2018_12_12_11_07_48.t.xml. Fri Dec 21 13:36:48 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:36:48 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:36:48 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_07.t.2018_12_12_11_07_48.t.xml Fri Dec 21 13:36:48 AEDT 2018 5425 identified spectra (10.4%) did not present a valid peptide. Fri Dec 21 13:36:48 AEDT 2018 7696 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:36:48 AEDT 2018 - 5.7% peptide mapping to both target and decoy. Fri Dec 21 13:36:48 AEDT 2018 - 27.7% peptide length less than 7 or greater than 30, Fri Dec 21 13:36:48 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:36:48 AEDT 2018 - 64.0% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:36:48 AEDT 2018 - 2.6% unrecognized modifications. Fri Dec 21 13:36:48 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_08.mzid. Fri Dec 21 13:36:50 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_08.mzid. Fri Dec 21 13:36:50 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_08.mgf Fri Dec 21 13:37:00 AEDT 2018 180419_E0022_P01_pool30_I_M06_08.mgf imported. Fri Dec 21 13:37:00 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:37:00 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:37:01 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_08.mzid Fri Dec 21 13:37:01 AEDT 2018 26812 identified spectra (40.9%) did not present a valid peptide. Fri Dec 21 13:37:01 AEDT 2018 32693 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:37:01 AEDT 2018 - 39.7% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:37:01 AEDT 2018 - 58.7% unrecognized modifications. Fri Dec 21 13:37:01 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_08.t.2018_12_12_11_10_43.t.xml. Fri Dec 21 13:37:51 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_08.t.2018_12_12_11_10_43.t.xml. Fri Dec 21 13:37:51 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:37:51 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:37:51 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_08.t.2018_12_12_11_10_43.t.xml Fri Dec 21 13:37:51 AEDT 2018 5256 identified spectra (10.2%) did not present a valid peptide. Fri Dec 21 13:37:51 AEDT 2018 7606 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:37:51 AEDT 2018 - 5.8% peptide mapping to both target and decoy. Fri Dec 21 13:37:51 AEDT 2018 - 28.6% peptide length less than 7 or greater than 30, Fri Dec 21 13:37:51 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:37:51 AEDT 2018 - 63.3% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:37:51 AEDT 2018 - 2.4% unrecognized modifications. Fri Dec 21 13:37:51 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_09.mzid. Fri Dec 21 13:37:54 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_09.mzid. Fri Dec 21 13:37:54 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_09.mgf Fri Dec 21 13:38:04 AEDT 2018 180419_E0022_P01_pool30_I_M06_09.mgf imported. Fri Dec 21 13:38:04 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:38:04 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:38:04 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_09.mzid Fri Dec 21 13:38:04 AEDT 2018 26999 identified spectra (41.0%) did not present a valid peptide. Fri Dec 21 13:38:04 AEDT 2018 32747 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:38:04 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:38:04 AEDT 2018 - 39.0% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:38:04 AEDT 2018 - 59.2% unrecognized modifications. Fri Dec 21 13:38:04 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_09.t.2018_12_12_11_13_42.t.xml. Fri Dec 21 13:38:58 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_09.t.2018_12_12_11_13_42.t.xml. Fri Dec 21 13:38:58 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:38:58 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:38:58 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_09.t.2018_12_12_11_13_42.t.xml Fri Dec 21 13:38:59 AEDT 2018 5288 identified spectra (10.3%) did not present a valid peptide. Fri Dec 21 13:38:59 AEDT 2018 7894 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:38:59 AEDT 2018 - 5.9% peptide mapping to both target and decoy. Fri Dec 21 13:38:59 AEDT 2018 - 30.7% peptide length less than 7 or greater than 30, Fri Dec 21 13:38:59 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:38:59 AEDT 2018 - 61.3% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:38:59 AEDT 2018 - 2.1% unrecognized modifications. Fri Dec 21 13:38:59 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_10.mzid. Fri Dec 21 13:39:01 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_10.mzid. Fri Dec 21 13:39:01 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_10.mgf Fri Dec 21 13:39:11 AEDT 2018 180419_E0022_P01_pool30_I_M06_10.mgf imported. Fri Dec 21 13:39:11 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:39:11 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:39:11 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_10.mzid Fri Dec 21 13:39:12 AEDT 2018 26815 identified spectra (41.3%) did not present a valid peptide. Fri Dec 21 13:39:12 AEDT 2018 32882 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:39:12 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:39:12 AEDT 2018 - 38.6% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:39:12 AEDT 2018 - 59.7% unrecognized modifications. Fri Dec 21 13:39:12 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_10.t.2018_12_12_11_16_28.t.xml. Fri Dec 21 13:39:58 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_10.t.2018_12_12_11_16_28.t.xml. Fri Dec 21 13:39:58 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:39:58 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:39:58 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_10.t.2018_12_12_11_16_28.t.xml Fri Dec 21 13:39:58 AEDT 2018 4967 identified spectra (9.9%) did not present a valid peptide. Fri Dec 21 13:39:58 AEDT 2018 7749 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:39:58 AEDT 2018 - 4.9% peptide mapping to both target and decoy. Fri Dec 21 13:39:58 AEDT 2018 - 33.5% peptide length less than 7 or greater than 30, Fri Dec 21 13:39:58 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:39:58 AEDT 2018 - 59.5% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:39:58 AEDT 2018 - 2.1% unrecognized modifications. Fri Dec 21 13:39:58 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_11.mzid. Fri Dec 21 13:40:00 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_11.mzid. Fri Dec 21 13:40:00 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_11.mgf Fri Dec 21 13:40:10 AEDT 2018 180419_E0022_P01_pool30_I_M06_11.mgf imported. Fri Dec 21 13:40:10 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:40:10 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:40:10 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_11.mzid Fri Dec 21 13:40:11 AEDT 2018 26648 identified spectra (40.4%) did not present a valid peptide. Fri Dec 21 13:40:11 AEDT 2018 32396 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:40:11 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:40:11 AEDT 2018 - 38.2% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:40:11 AEDT 2018 - 59.8% unrecognized modifications. Fri Dec 21 13:40:11 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_11.t.2018_12_12_11_19_25.t.xml. Fri Dec 21 13:41:01 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_11.t.2018_12_12_11_19_25.t.xml. Fri Dec 21 13:41:01 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:41:01 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:41:01 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_11.t.2018_12_12_11_19_25.t.xml Fri Dec 21 13:41:01 AEDT 2018 5102 identified spectra (9.9%) did not present a valid peptide. Fri Dec 21 13:41:01 AEDT 2018 7817 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:41:01 AEDT 2018 - 5.1% peptide mapping to both target and decoy. Fri Dec 21 13:41:01 AEDT 2018 - 32.0% peptide length less than 7 or greater than 30, Fri Dec 21 13:41:01 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:41:01 AEDT 2018 - 60.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:41:01 AEDT 2018 - 2.5% unrecognized modifications. Fri Dec 21 13:41:01 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_12.mzid. Fri Dec 21 13:41:04 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_12.mzid. Fri Dec 21 13:41:04 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_12.mgf Fri Dec 21 13:41:14 AEDT 2018 180419_E0022_P01_pool30_I_M06_12.mgf imported. Fri Dec 21 13:41:14 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:41:14 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:41:14 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_12.mzid Fri Dec 21 13:41:14 AEDT 2018 26556 identified spectra (40.9%) did not present a valid peptide. Fri Dec 21 13:41:14 AEDT 2018 32170 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:41:14 AEDT 2018 - 1.1% peptide mapping to both target and decoy. Fri Dec 21 13:41:14 AEDT 2018 - 38.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:41:14 AEDT 2018 - 60.0% unrecognized modifications. Fri Dec 21 13:41:14 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_12.t.2018_12_12_10_44_16.t.xml. Fri Dec 21 13:42:02 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_12.t.2018_12_12_10_44_16.t.xml. Fri Dec 21 13:42:02 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:42:02 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:42:03 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_12.t.2018_12_12_10_44_16.t.xml Fri Dec 21 13:42:03 AEDT 2018 5122 identified spectra (10.0%) did not present a valid peptide. Fri Dec 21 13:42:03 AEDT 2018 7369 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:42:03 AEDT 2018 - 5.7% peptide mapping to both target and decoy. Fri Dec 21 13:42:03 AEDT 2018 - 27.8% peptide length less than 7 or greater than 30, Fri Dec 21 13:42:03 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:42:03 AEDT 2018 - 63.6% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:42:03 AEDT 2018 - 2.8% unrecognized modifications. Fri Dec 21 13:42:03 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_13.mzid. Fri Dec 21 13:42:05 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_13.mzid. Fri Dec 21 13:42:05 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_13.mgf Fri Dec 21 13:42:14 AEDT 2018 180419_E0022_P01_pool30_I_M06_13.mgf imported. Fri Dec 21 13:42:14 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:42:15 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:42:15 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_13.mzid Fri Dec 21 13:42:16 AEDT 2018 25790 identified spectra (39.4%) did not present a valid peptide. Fri Dec 21 13:42:16 AEDT 2018 31333 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:42:16 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:42:16 AEDT 2018 - 39.0% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:42:16 AEDT 2018 - 59.0% unrecognized modifications. Fri Dec 21 13:42:16 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_13.t.2018_12_12_11_22_27.t.xml. Fri Dec 21 13:43:07 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_13.t.2018_12_12_11_22_27.t.xml. Fri Dec 21 13:43:07 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:43:07 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:43:07 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_13.t.2018_12_12_11_22_27.t.xml Fri Dec 21 13:43:07 AEDT 2018 4984 identified spectra (9.6%) did not present a valid peptide. Fri Dec 21 13:43:07 AEDT 2018 7182 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:43:07 AEDT 2018 - 6.1% peptide mapping to both target and decoy. Fri Dec 21 13:43:07 AEDT 2018 - 27.8% peptide length less than 7 or greater than 30, Fri Dec 21 13:43:07 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:43:07 AEDT 2018 - 63.6% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:43:07 AEDT 2018 - 2.5% unrecognized modifications. Fri Dec 21 13:43:07 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_14.mzid. Fri Dec 21 13:43:09 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_14.mzid. Fri Dec 21 13:43:09 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_14.mgf Fri Dec 21 13:43:19 AEDT 2018 180419_E0022_P01_pool30_I_M06_14.mgf imported. Fri Dec 21 13:43:19 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:43:19 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:43:19 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_14.mzid Fri Dec 21 13:43:20 AEDT 2018 26313 identified spectra (40.0%) did not present a valid peptide. Fri Dec 21 13:43:20 AEDT 2018 31971 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:43:20 AEDT 2018 - 39.3% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:43:20 AEDT 2018 - 58.9% unrecognized modifications. Fri Dec 21 13:43:20 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_14.t.2018_12_12_10_47_09.t.xml. Fri Dec 21 13:44:09 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_14.t.2018_12_12_10_47_09.t.xml. Fri Dec 21 13:44:09 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:44:09 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:44:09 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_14.t.2018_12_12_10_47_09.t.xml Fri Dec 21 13:44:10 AEDT 2018 5204 identified spectra (10.0%) did not present a valid peptide. Fri Dec 21 13:44:10 AEDT 2018 7032 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:44:10 AEDT 2018 - 5.0% peptide mapping to both target and decoy. Fri Dec 21 13:44:10 AEDT 2018 - 24.0% peptide length less than 7 or greater than 30, Fri Dec 21 13:44:10 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:44:10 AEDT 2018 - 68.8% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:44:10 AEDT 2018 - 2.3% unrecognized modifications. Fri Dec 21 13:44:10 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_15.mzid. Fri Dec 21 13:44:12 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_15.mzid. Fri Dec 21 13:44:12 AEDT 2018 Importing 180419_E0022_P01_pool30_I_M06_15.mgf Fri Dec 21 13:44:23 AEDT 2018 180419_E0022_P01_pool30_I_M06_15.mgf imported. Fri Dec 21 13:44:23 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:44:23 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:44:23 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_15.mzid Fri Dec 21 13:44:24 AEDT 2018 25099 identified spectra (38.4%) did not present a valid peptide. Fri Dec 21 13:44:24 AEDT 2018 30384 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:44:24 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:44:24 AEDT 2018 - 39.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:44:24 AEDT 2018 - 58.9% unrecognized modifications. Fri Dec 21 13:44:24 AEDT 2018 Parsing 180419_E0022_P01_pool30_I_M06_15.t.2018_12_12_11_25_23.t.xml. Fri Dec 21 13:45:14 AEDT 2018 Loading spectra for 180419_E0022_P01_pool30_I_M06_15.t.2018_12_12_11_25_23.t.xml. Fri Dec 21 13:45:14 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:45:14 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:45:14 AEDT 2018 Importing PSMs from 180419_E0022_P01_pool30_I_M06_15.t.2018_12_12_11_25_23.t.xml Fri Dec 21 13:45:14 AEDT 2018 5195 identified spectra (10.0%) did not present a valid peptide. Fri Dec 21 13:45:14 AEDT 2018 6956 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:45:14 AEDT 2018 - 7.8% peptide mapping to both target and decoy. Fri Dec 21 13:45:14 AEDT 2018 - 22.6% peptide length less than 7 or greater than 30, Fri Dec 21 13:45:14 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:45:14 AEDT 2018 - 66.6% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:45:14 AEDT 2018 - 3.0% unrecognized modifications. Fri Dec 21 13:45:14 AEDT 2018 Parsing F038740.dat. Fri Dec 21 13:45:27 AEDT 2018 Loading spectra for F038740.dat. Fri Dec 21 13:45:27 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:45:28 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:45:28 AEDT 2018 Importing PSMs from F038740.dat Fri Dec 21 13:45:29 AEDT 2018 3661 identified spectra (6.0%) did not present a valid peptide. Fri Dec 21 13:45:29 AEDT 2018 45519 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:45:29 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:45:29 AEDT 2018 - 32.0% peptide length less than 7 or greater than 30, Fri Dec 21 13:45:29 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:45:29 AEDT 2018 - 12.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:45:29 AEDT 2018 - 54.3% unrecognized modifications. Fri Dec 21 13:45:29 AEDT 2018 Parsing F038742.dat. Fri Dec 21 13:45:45 AEDT 2018 Loading spectra for F038742.dat. Fri Dec 21 13:45:45 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:45:45 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:45:45 AEDT 2018 Importing PSMs from F038742.dat Fri Dec 21 13:45:47 AEDT 2018 4001 identified spectra (6.7%) did not present a valid peptide. Fri Dec 21 13:45:47 AEDT 2018 33681 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:45:47 AEDT 2018 - 24.7% peptide length less than 7 or greater than 30, Fri Dec 21 13:45:47 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:45:47 AEDT 2018 - 12.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:45:47 AEDT 2018 - 62.0% unrecognized modifications. Fri Dec 21 13:45:47 AEDT 2018 Parsing F038743.dat. Fri Dec 21 13:45:57 AEDT 2018 Loading spectra for F038743.dat. Fri Dec 21 13:45:57 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:45:58 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:45:58 AEDT 2018 Importing PSMs from F038743.dat Fri Dec 21 13:45:59 AEDT 2018 3910 identified spectra (6.4%) did not present a valid peptide. Fri Dec 21 13:45:59 AEDT 2018 43222 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:45:59 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:45:59 AEDT 2018 - 32.7% peptide length less than 7 or greater than 30, Fri Dec 21 13:45:59 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:45:59 AEDT 2018 - 11.8% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:45:59 AEDT 2018 - 54.2% unrecognized modifications. Fri Dec 21 13:45:59 AEDT 2018 Parsing F038744.dat. Fri Dec 21 13:46:08 AEDT 2018 Loading spectra for F038744.dat. Fri Dec 21 13:46:08 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:46:08 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:46:09 AEDT 2018 Importing PSMs from F038744.dat Fri Dec 21 13:46:09 AEDT 2018 3783 identified spectra (6.3%) did not present a valid peptide. Fri Dec 21 13:46:09 AEDT 2018 47609 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:46:09 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:46:09 AEDT 2018 - 36.0% peptide length less than 7 or greater than 30, Fri Dec 21 13:46:09 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:46:09 AEDT 2018 - 11.6% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:46:09 AEDT 2018 - 51.1% unrecognized modifications. Fri Dec 21 13:46:09 AEDT 2018 Parsing F038745.dat. Fri Dec 21 13:46:19 AEDT 2018 Loading spectra for F038745.dat. Fri Dec 21 13:46:19 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:46:19 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:46:20 AEDT 2018 Importing PSMs from F038745.dat Fri Dec 21 13:46:20 AEDT 2018 4153 identified spectra (7.0%) did not present a valid peptide. Fri Dec 21 13:46:20 AEDT 2018 47459 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:46:20 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:46:20 AEDT 2018 - 37.2% peptide length less than 7 or greater than 30, Fri Dec 21 13:46:20 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:46:20 AEDT 2018 - 11.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:46:20 AEDT 2018 - 50.1% unrecognized modifications. Fri Dec 21 13:46:20 AEDT 2018 Parsing F038746.dat. Fri Dec 21 13:46:33 AEDT 2018 Loading spectra for F038746.dat. Fri Dec 21 13:46:33 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:46:34 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:46:34 AEDT 2018 Importing PSMs from F038746.dat Fri Dec 21 13:46:35 AEDT 2018 4355 identified spectra (7.5%) did not present a valid peptide. Fri Dec 21 13:46:35 AEDT 2018 47444 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:46:35 AEDT 2018 - 1.1% peptide mapping to both target and decoy. Fri Dec 21 13:46:35 AEDT 2018 - 40.3% peptide length less than 7 or greater than 30, Fri Dec 21 13:46:35 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:46:35 AEDT 2018 - 11.6% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:46:35 AEDT 2018 - 47.1% unrecognized modifications. Fri Dec 21 13:46:35 AEDT 2018 Parsing F038747.dat. Fri Dec 21 13:46:44 AEDT 2018 Loading spectra for F038747.dat. Fri Dec 21 13:46:45 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:46:45 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:46:45 AEDT 2018 Importing PSMs from F038747.dat Fri Dec 21 13:46:46 AEDT 2018 4456 identified spectra (7.6%) did not present a valid peptide. Fri Dec 21 13:46:46 AEDT 2018 48393 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:46:46 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:46:46 AEDT 2018 - 42.1% peptide length less than 7 or greater than 30, Fri Dec 21 13:46:46 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:46:46 AEDT 2018 - 10.9% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:46:46 AEDT 2018 - 45.8% unrecognized modifications. Fri Dec 21 13:46:46 AEDT 2018 Parsing F038748.dat. Fri Dec 21 13:46:55 AEDT 2018 Loading spectra for F038748.dat. Fri Dec 21 13:46:55 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:46:56 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:46:56 AEDT 2018 Importing PSMs from F038748.dat Fri Dec 21 13:46:57 AEDT 2018 3974 identified spectra (6.8%) did not present a valid peptide. Fri Dec 21 13:46:57 AEDT 2018 48206 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:46:57 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:46:57 AEDT 2018 - 43.9% peptide length less than 7 or greater than 30, Fri Dec 21 13:46:57 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:46:57 AEDT 2018 - 11.2% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:46:57 AEDT 2018 - 43.6% unrecognized modifications. Fri Dec 21 13:46:57 AEDT 2018 Parsing F038749.dat. Fri Dec 21 13:47:07 AEDT 2018 Loading spectra for F038749.dat. Fri Dec 21 13:47:07 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:47:07 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:47:07 AEDT 2018 Importing PSMs from F038749.dat Fri Dec 21 13:47:08 AEDT 2018 3943 identified spectra (6.7%) did not present a valid peptide. Fri Dec 21 13:47:08 AEDT 2018 51324 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:47:08 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:47:08 AEDT 2018 - 44.5% peptide length less than 7 or greater than 30, Fri Dec 21 13:47:08 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:47:08 AEDT 2018 - 10.8% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:47:08 AEDT 2018 - 43.4% unrecognized modifications. Fri Dec 21 13:47:08 AEDT 2018 Parsing F038750.dat. Fri Dec 21 13:47:18 AEDT 2018 Loading spectra for F038750.dat. Fri Dec 21 13:47:18 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:47:18 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:47:18 AEDT 2018 Importing PSMs from F038750.dat Fri Dec 21 13:47:19 AEDT 2018 3952 identified spectra (6.8%) did not present a valid peptide. Fri Dec 21 13:47:19 AEDT 2018 53463 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:47:19 AEDT 2018 - 1.2% peptide mapping to both target and decoy. Fri Dec 21 13:47:19 AEDT 2018 - 46.1% peptide length less than 7 or greater than 30, Fri Dec 21 13:47:19 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:47:19 AEDT 2018 - 10.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:47:19 AEDT 2018 - 42.4% unrecognized modifications. Fri Dec 21 13:47:19 AEDT 2018 Parsing F038751.dat. Fri Dec 21 13:47:29 AEDT 2018 Loading spectra for F038751.dat. Fri Dec 21 13:47:29 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:47:29 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:47:30 AEDT 2018 Importing PSMs from F038751.dat Fri Dec 21 13:47:30 AEDT 2018 3986 identified spectra (6.8%) did not present a valid peptide. Fri Dec 21 13:47:30 AEDT 2018 52677 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:47:30 AEDT 2018 - 1.4% peptide mapping to both target and decoy. Fri Dec 21 13:47:30 AEDT 2018 - 45.9% peptide length less than 7 or greater than 30, Fri Dec 21 13:47:30 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:47:30 AEDT 2018 - 10.3% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:47:30 AEDT 2018 - 42.4% unrecognized modifications. Fri Dec 21 13:47:30 AEDT 2018 Parsing F038752.dat. Fri Dec 21 13:47:40 AEDT 2018 Loading spectra for F038752.dat. Fri Dec 21 13:47:40 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:47:40 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:47:40 AEDT 2018 Importing PSMs from F038752.dat Fri Dec 21 13:47:41 AEDT 2018 3920 identified spectra (6.8%) did not present a valid peptide. Fri Dec 21 13:47:41 AEDT 2018 49053 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:47:41 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:47:41 AEDT 2018 - 42.3% peptide length less than 7 or greater than 30, Fri Dec 21 13:47:41 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:47:41 AEDT 2018 - 11.1% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:47:41 AEDT 2018 - 45.3% unrecognized modifications. Fri Dec 21 13:47:41 AEDT 2018 Parsing F038754.dat. Fri Dec 21 13:47:51 AEDT 2018 Loading spectra for F038754.dat. Fri Dec 21 13:47:51 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:47:51 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:47:51 AEDT 2018 Importing PSMs from F038754.dat Fri Dec 21 13:47:52 AEDT 2018 3988 identified spectra (6.8%) did not present a valid peptide. Fri Dec 21 13:47:52 AEDT 2018 47236 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:47:52 AEDT 2018 - 1.3% peptide mapping to both target and decoy. Fri Dec 21 13:47:52 AEDT 2018 - 39.5% peptide length less than 7 or greater than 30, Fri Dec 21 13:47:52 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:47:52 AEDT 2018 - 11.3% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:47:52 AEDT 2018 - 47.8% unrecognized modifications. Fri Dec 21 13:47:52 AEDT 2018 Parsing F038756.dat. Fri Dec 21 13:48:01 AEDT 2018 Loading spectra for F038756.dat. Fri Dec 21 13:48:02 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:48:02 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:48:02 AEDT 2018 Importing PSMs from F038756.dat Fri Dec 21 13:48:03 AEDT 2018 4054 identified spectra (6.8%) did not present a valid peptide. Fri Dec 21 13:48:03 AEDT 2018 45360 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:48:03 AEDT 2018 - 1.0% peptide mapping to both target and decoy. Fri Dec 21 13:48:03 AEDT 2018 - 37.6% peptide length less than 7 or greater than 30, Fri Dec 21 13:48:03 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:48:03 AEDT 2018 - 11.5% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:48:03 AEDT 2018 - 50.0% unrecognized modifications. Fri Dec 21 13:48:03 AEDT 2018 Parsing F038757.dat. Fri Dec 21 13:48:13 AEDT 2018 Loading spectra for F038757.dat. Fri Dec 21 13:48:13 AEDT 2018 Collecting peptides to map. Fri Dec 21 13:48:13 AEDT 2018 Mapping peptides to proteins. Fri Dec 21 13:48:14 AEDT 2018 Importing PSMs from F038757.dat Fri Dec 21 13:48:14 AEDT 2018 3916 identified spectra (6.6%) did not present a valid peptide. Fri Dec 21 13:48:14 AEDT 2018 42489 of the best scoring peptides were excluded by the import filters: Fri Dec 21 13:48:14 AEDT 2018 - 1.6% peptide mapping to both target and decoy. Fri Dec 21 13:48:14 AEDT 2018 - 36.3% peptide length less than 7 or greater than 30, Fri Dec 21 13:48:14 AEDT 2018 or number of missed cleavage sites outside of the range [0-2]. Fri Dec 21 13:48:14 AEDT 2018 - 11.4% peptide presenting high mass or isotopic deviation. Fri Dec 21 13:48:14 AEDT 2018 - 50.7% unrecognized modifications. Fri Dec 21 13:48:14 AEDT 2018 File import completed. 2638224 first hits imported (7780005 secondary) from 1003257 spectra. Fri Dec 21 13:48:14 AEDT 2018 [2067596 first hits passed the initial filtering] Fri Dec 21 13:48:14 AEDT 2018 Computing assumptions probabilities. Fri Dec 21 13:48:17 AEDT 2018 Saving assumptions probabilities. Fri Dec 21 13:48:40 AEDT 2018 Selecting best peptide per spectrum. Fri Dec 21 13:49:05 AEDT 2018 Computing PSM probabilities. Fri Dec 21 13:49:05 AEDT 2018 Scoring PTMs in PSMs (D-score and PhosphoRS) Fri Dec 21 13:53:15 AEDT 2018 Resolving peptide inference issues. Fri Dec 21 14:06:14 AEDT 2018 Saving probabilities, building peptides and proteins.
Fri Dec 21 14:06:16 AEDT 2018 Importing Data Canceled! Fri Dec 21 14:06:16 AEDT 2018 An error occurred while loading the identification files. Fri Dec 21 14:06:16 AEDT 2018 Please see the error log (Help Menu > Bug Report) for details.