comprna / METEORE

Automatic DNA methylation detection from nanopore tools and their consensus model
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Question: Can this pipeline be used for bacterial methylation detectin. #31

Open martinastoycheva opened 2 years ago

martinastoycheva commented 2 years ago

Hello,

In E.coli Dcm methyltransferases methylate the C5 position of the second cytosine in the sequences CCAGG and CCTGG. I was wondering if I could use your tool for detecting this in bacteria or is it somehow restricted to the CpG sites in eukaryotes?

Thank you! Martina

EduEyras commented 2 years ago

Hi Martina,

We decided to focus on CpG sites, which are the most common methylation sites in humans, but everything should be applicable to non-CG sites. However, we have not compared non-CG site predictions with bisulfite data.

best

Eduardo

On Tue, 21 Jun 2022 at 22:37, Martina Stoycheva @.***> wrote:

Hello,

In E.coli Dcm methyltransferases methylate the C5 position of the second cytosine in the sequences CCAGG and CCTGG. I was wondering if I could use your tool for detecting this in bacteria or is it somehow restricted to the CpG sites in eukaryotes?

Thank you! Martina

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