constantAmateur / SoupX

R package to quantify and remove cell free mRNAs from droplet based scRNA-seq data
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Loading Data using load10X function #15

Closed cswoboda closed 4 years ago

cswoboda commented 4 years ago

Hello!

I've been trying to load my data into R, however because I received the cellranger output through a dropbox, I don't think the function can establish a connection to read the files. Could someone who has successfully loaded data through this function share with me their file names and what their output looked like for cellranger V3? Here's my session info and errors:

dataDirs = c('E:/Casey/data/twentyfour_Month_Raw_Data/twenty_four_month_data/raw_data_matrix_included') scl = load10X(dataDirs, keepDroplets = TRUE) Loading data for 10X channel Channel1 from E:/Casey/data/twentyfour_Month_Raw_Data/twenty_four_month_data/raw_data_matrix_included Error in open.connection(file) : cannot open the connection In addition: Warning message: In open.connection(file) : cannot open file 'E:/Casey/data/twentyfour_Month_Raw_Data/twenty_four_month_data/raw_data_matrix_included/raw_feature_bc_matrix/matrix.mtx.gz': No such file or directory

R version 3.6.0 (2019-04-26) Platform: x86_64-w64-mingw32/x64 (64-bit) Running under: Windows 10 x64 (build 17134)

Thanks!