Closed gongyuTang123 closed 1 year ago
Dear user, Sorry for not getting back to you sooner. The question that you bring is quite interesting and can have different ways of being approached:
--pon
in the Step4.2 of the tumour sample. --min_samples 1
. You can then use this PoN for the tumour sample. I hope it helps, Fran
I am trying to detect somatic mutations from my single-cell RNA-seq data for lung cancer samples. I knew SComatic relies on different cell types' data to remove possible germline mutations and artifacts. But I happened to have the matched normal single-cell data isolated from the same cancer patient. How could these data be used to help generate better results by SComatic?
Should I use the 'scripts/PoN/PoN.py' to generate a novel PON for each patient or for each cell subpopulation and use it as a reference to remove possible germline mutations?