cougarlj / COMPSRA

COMPSRA: a COMprehensive Platform for Small RNA-Seq data Analysis
https://regepi.bwh.harvard.edu/circurna/
GNU General Public License v3.0
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Issue during alignment/installation #38

Closed jackbrougher closed 2 years ago

jackbrougher commented 2 years ago

Hello,

Thank you for providing the COMPSRA resource for usage.

I'm in the process of installing and checking the installation process on an EC2 AWS server. Currently I'm running into an issue at the example alignment step, and I'm assuming it has to do with the previous installation of STAR. Error message copied below, any thoughts or suggestions?

/newvolume/COMPSRA/bundle_v1/plug/star/STAR-2.5.3a/bin/Linux_x86_64/STAR: error while loading shared libraries: libgomp.so.1: cannot open shared object file: No such file or directory Fail to call: /newvolume/COMPSRA/bundle_v1/plug/star/STAR-2.5.3a/bin/Linux_x86_64/STAR --runThreadN 4 --runMode alignReads --outSAMtype BAM Unsorted --outSAMattributes Standard --readFilesCommand zcat --outSAMunmapped Within --outReadsUnmapped None --alignEndsType Local --outFilterMismatchNmax 1 --alignIntronMax 1 --alignIntronMin 2 --outFilterMultimapScoreRange 1 --outFilterScoreMinOverLread 0.66 --outFilterMatchNminOverLread 0.66 --outFilterMismatchNoverLmax 0.05 --outFilterMatchNmin 16 --outFilterMultimapNmax 1000000 --genomeDir /newvolume/COMPSRA/bundle_v1/db/star/hg38 --readFilesIn /newvolume/COMPSRA/example_out/sample01/sample01_17to50_FitRead.fastq.gz --outFileNamePrefix /newvolume/COMPSRA/example_out/sample01/sample01_17to50_FitReadSTAR

jackbrougher commented 2 years ago

Figured it out

cougarlj commented 2 years ago

That's great. If you have any problem about using COMPSRA, please let me know.