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cox-labs / CoxLab_Bug_Reporting

MaxQuant and Perseus Bug Reporting
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Adding Isotopic corrections for 18plex TMT is not working properly? #14

Closed ChrisRampitsch closed 1 year ago

ChrisRampitsch commented 1 year ago

Describe the bug Adding Isotopic Label Corrections is not possible for 18plex

To Reproduce In v2.2.0.0 Go to: "Group Specific Parameters" -> "Type" = Reporter Ion MS2. Select any TMT plex (e.g. 18plex) Then press Edit for any label Then for any correction factor (e.g. -2[%]) press [+] and enter values for -2x 13C and -13C-15N (e.g. select 1% and 2% respectively) These values are not saved into the main Table upon closing the Define Isobaric Label window.

It is therefore only possible to specify a single value for each correction, not two as given by the Thermo Product Data Sheet for the TMT kit.

Expected behavior

  1. In the "Define Isobaric Label" pop-up window, Define "Internal Label" and "Terminal Label" as "TMTpro 18plex-Lys134C" and "TMTpro 18plex-Lys135N" respectively.
  2. This populates the Isobaric Labels table (previous window) correctly, as it should, after you press OK.
  3. Entering any values for Correction Factor (in the "Define Isobaric Label" pop-up window) will also work, but NOT if you use the [+] button to enter 2 values per isotope. In this case the Isobaric Labels table (previous window) stays blank.

Screenshots

Maxquant 18plex

Desktop (please complete the following information):

Additional context I have contacted another lab bmuseliu@uoguelph.ca as I know they also use 18plex. They report the same issue. Happy new Year!

chris.rampitsch@agr.gc.ca

Ting-PKU commented 1 year ago

We have encountered the same issue. We tried the newest version 2.3.1 of maxquant.

danielaferretti1992 commented 1 year ago

Hi Chris, please try the latest version. We fixed this issue. Best, Daniela

ChrisRampitsch commented 1 year ago

Thanks! It is working perfectly now!

C.

From: Daniela @.> Sent: Tuesday, July 11, 2023 12:09 PM To: cox-labs/CoxLab_Bug_Reporting @.> Cc: Rampitsch, Chris (AAFC/AAC) @.>; Author @.> Subject: Re: [cox-labs/CoxLab_Bug_Reporting] Adding Isotopic corrections for 18plex TMT is not working properly? (Issue #14)

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Hi Chris, please try the latest version. We fixed this issue. Best, Daniela

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